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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pME-kpm (#RDB07697)

Expression vector of human LATS2 kinase

Clone info. Expression vector of human LATS2 kinase.
Vector backbone pME18S (Plasmid)
Selectable markers Amp^r
Gene/insert name Human KPM (LATS2)
Depositor|Developer Hori, Toshiyuki |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Hori, T., Oncogene, 19, 3101-3109 (2000))
提供案内 (日本国内) [open/close]

MTAに書く使用条件 利用者は、研究成果の公表にあたって謝辞の表明を必要とする。　利用者は、研究成果の公表にあたって譲渡者の指定する文献を引用する（Hori, T., Oncogene, 19, 3101-3109 (2000))。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB07697 pME-kpm DNA solution Please contact us.

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pME-kpm was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07697).

Reference section:

Hori, T., Takaori-Kondo, A., Kamikubo, Y., Uchiyama, T., Molecular cloning of a novel human protein kinase, kpm, that is homologous to warts/lats, a Drosophila tumor suppressor. Oncogene, 19, 3101-3109 (2000). PMID 10871863. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14126_A6Dcp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: SRapro_F
Sequence file: RDB14126_A6Dca.seq check
>07697_14126_A6Dc_SRapro_F_A12_03.ab1
    1 ATGGTAGTCT TGCTCACTCT ACGTCTTTGT TTCGTTTTCT GTTCTGCGCC GTTACAGATC
   61 CAAGCTCTGA AAAACCAGAA AGTTAACTGG TAAGTTTAGT CTTTTTGTCT TTTATTTCAG
  121 GTCCCGGATC CGGTGGTGGT GCAAATCAAA GAACTGCTCC TCAGTGGATG TTGCCTTTAC
  181 TTCTAGGCCT GTACGGAAGT GTTACTTCTG CTCTAAAAGC TGCGGAATTC CGGAATTTCC
  241 TCGAGATCCA TTGTGCTGGA AAGGCCCCGG GAAGATGGAG CAGTCGCCGT CCACGCCACC
  301 GCCGCCGCCC GGGGCTCCCC CGTCCCTGCG GGGCCAGCAG CAGCTCCAGC CACCAGTGCC
  361 CGGTCTCCCG GCGCGAGAGG CCCGGGAGCC GCCGGCCAGG ACGCCCCCGA GGGTGTAGAC
  421 CGCGCCCCTG GAGAGAGTGA TAATCTTCAA AATGAAGACT TTGGAAAATT TTAGGTTCTC
  481 TATAGGAACT ACAAAAATGG AAGGAAAGAA CATTTTCAAA AGGAAATTAT TTTGAAAGTA
  541 TGTTTACAAC AAACTGATAC TATTGACAGT TTTTTTTTTT AAATAATAAA ACACTTTAAG
  601 AAGATTGTAT TTATGGTAAA AGGAAACTGG ACTAACAATG AGGCCAAAGA CTTTTCCTGC
  661 CACGACTTAT TCTGGAAATA GCCGGCAGCG ACTGCAAGAG ATTCGTGAGG GGTTAAAACA
  721 GCCATCCAAG TCTTCGGTTC AGGGGCTACC CGCAGGACCA AACAGTGACA CTTCCCTGGA
  781 TGCCAAAGTC CTGGGGAGCA AAGATGCCAC CAGGCAGCAG CAGCAGATGA GAGCCACCCC
  841 AAAGTTCGGA CCTTATCAGA AAGCCTTGAG GGAAATCAGA TATTCCTTGT TGCCTTTTGC
  901 TAATGAATCG GGCACCTCTG CAGCTGCAGA AGTGAACCGG CAAATGCTGC AGAACTGGTG
  961 AACGCAAGGA TGCGACCAGG AGATGGCTGG CCGAGCTCTC AAGCAGACTG GCAGCAGGAG
 1021 CATCGAGCCC GCCCTGTAGT ACATCAGCCA AGATGGGCTA CCTGCACCCC GAAGGAATGG
1081 AGCAGATGAT GCGGGTTCAT TAAGGCAAGA ACCTCTCCCC AAGGGA
//
Primer: ME-1290Rv
Sequence file: RDB14126_A6Dcb.seq check
>07697_14126_A6Dc_ME-1290Rv_B12_06.ab1
    1 AGCATTCATT TTATGTTTCA GGTTCAGGGG GAGGTGTGGG AGGTTTTTTC TCTAGACTAG
   61 TCTAGCGGCC GCGACCTGCA GGCGCAGAAC TGGTAGGTAT GGAAGATCCC TCGAGATCCA
  121 TTGTGCTGGA AAGTGCACAG AGATTTCTCA TCAATGTTCT TCAGTTTTTA TGTCTTTTCC
  181 TAAATGTGAA TAAGTGCTAT GGATAAAATA CAAATGTAGA AAATAACAGC AGCATGATTT
  241 GTCAAAGTTA ATCCCTATAA TTTAGTAAGA AAAAATGGAT ATAAACAAAA TAAGTGCTCT
  301 TTCTAAACTG TACTAAATTT TTAAAAATAT TGTTTTAATG CAGTGAAGGT CCTGAAAAGC
  361 CTATTGAAAG CGATGCTGAG TCCTGTTTTC AAAAGTGTCC TGTTTGGGTT TTCTTGGTGA
  421 AGAGCAGAAT TTCAAGTGAA GTAATCGACG GACTAATTTA AAACAAAACA GCCCTCGGCT
  481 TCCCTATTGG CCTGTGAGGG CACCGGCTCC GGGACCCTGA CCTGGGAGGC AGCGAGTGGT
  541 GGGGGTGCCT GGCCCCCATC TACACGTACA CAGGCTGGCA GCCTTCAGTC TGATCCACCA
  601 GATCAGAGCT TTCTAAATCT GAGCTCTCAG CCTGTGAAGC TTCTGCTCCT GAAGGCTTTG
  661 GGCATCGAAA GGGGTAGCCA TTGTCATCAA AGAACCTTCG GAAGGTGAAT TCGTAAAATG
  721 CGTGCTCAGG ATGCTTGTTA TTGGGCGAGG TGAGTGTGTC CCAGGCCTTG GTGCTACCTT
  781 CGCTGGCATC GTTCCAAGGG CTTTCTTCAT CTACGGGGTC GAAATTCGAG GTGTCCATGG
  841 GGTGGCTGAT GGTGGGAACG TAGGGGGCTG GCTGCTTCCG GATGTCACTG GAGAAGTCAA
  901 TGGCGCTGAA GAAGGGGTGG GCCTTCAGGT CATCGGCCCC ATTCCGCCCC AGGCGGTGGT
  961 CTGCGGAGCA GCACAGCTTG GTGATGAGGT CCCTGGCCCT CAGGCTCAGC TTTCACTGGC
 1021 TGGAATGTGA GCGTGTTCTC CAGTGATCAC CTTCAGCTGG GTTCTGTGGA GTAAGTTGCC
1081 AAAAAGGCGG CTGCTCACCA GCATCTCGGA GAGATCACTC GACACTCCAC CAG
//
Primer: Lats2#11
Sequence file: RDB14126_A6Dcc.seq check
>07697_14126_A6Dc_Lats2#11_C12_09.ab1
    1 CAAGTACGGA GCTGCAGCTG GAGCAGAATG GCCAAAGCTG GACTCTGTGA AGCTGAGCAG
   61 GAGCAGATGC GGAAGATCCT CTACCAGAAA GAGTCTAATT ACAACAGGTT AAAGAGGGCC
  121 AAGATGGACA AGTCTATGTT TGTCAAGATC AAAACCCTGG GGATCGGTGC CTTTGGAGAA
  181 GTGTGCCTTG CTTGTAAGGT GGACACTCAC GCCCTGTACG CCATGAAGAC CCTAAGGAAA
  241 AAGGATGTCC TGAACCGGAA TCAGGTGGCC CACGTCAAGG CCGAGAGGGA CATCCTGGCC
  301 GAGGCAGACA ATGAGTGGGT GGTCAAACTC TACTACTCCT TCCAAGACAA AGACAGCCTG
  361 TACTTTGTGA TGGACTACAT CCCTGGTGGG GACATGATGA GCCTGCTGAT CCGGATGGAG
  421 GTCTTCCCTG AGCACCTGGC CCGGTTCTAC ATCGCAGAGC TGACTTTGGC CATTGAGAGT
  481 GTCCACAAGA TGGGCTTCAT CCACCGAGAC ATCAAGCCTG ATAACATTTT GATAGATCTG
  541 GATGGTCACA TTAAACTCAC AGATTTCGGC CTCTGCACTG GGTTCAGGTG GACTCACAAT
  601 TCCAAATATT ACCAGAAAGG GAGCCATGTC AGACAGGACA GCATGGAGCC CAGCGACCTC
  661 TGGGATGATG TGTCTAACTG TCGGTGTGGG GACAGGCTGA AGACCCTAGA GCAGAGGGCG
  721 CGGAAGCAGC ACCAGAGGTG CCTGGCACAT TCACTGGTGG GGACTCCAAA CTACATCGCA
  781 CCCGAGGTGC TCCTCCGCAA AGGGTACACT CAACTCTGTG ACTGGTGGAG TGTTGGAGTG
  841 ATTCTCTTCG AGATGCTGGT GGGGCAGCCG CCCTTTTTGG CACCTACTCC CACAGAAACC
  901 CAGCTGAAAG TGATCAACTG GGAGAACACG CTCCACATTC CAGCCCAGGT GAAGCTGAGC
  961 CCTGAGGCCA GGGACCTCAT CACCAAGCTG TGCTGCTCCG CAGACCCACC GCCTGGGGCG
 1021 GGAAATGGGG CCGATGACTG AAGCCCATCC CTTCTTCAGC GCCATGACTT CTCAGTGACA
1081 TCCGGAAGCA GCAGCCCCTA CGATCCCACA TCAGCCCAAC CCCCAAG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Hori, T., Molecular cloning of a novel human protein kinase, kpm, that is homologous to warts/lats, a Drosophila tumor suppressor. Oncogene, 19, 3101-3109 (2000). PMID 10871863. [link to RRC of NBRP]