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pGL4-phVEGFA

Promoter collection, Human VEGFA promoter

Catalog number RDB07681
Resource name pGL4-phVEGFA
Alternative name U2749(2127)-8
Clone info. PCR amplified human VEGFA promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 43769068 to 43770372(NC_000006.12); relatively -1141 to +164, where +1 corresponds to 1 nt of NM_001025366.2.
Comment PCR cloning, forward primer: 5’- GGCGGGTAGGTTTGAATCAT -3’; reverse primer : 5’- AGGGATAAAACCCGGATCAA -3’. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human vascular endothelial growth factor A genomic DNA
Depositor|Developer DNA Bank, | Kishikawa, Shotaro |
Other clones in our bank
External Database
Human vascular endothelial growth factor A
          Reference sequence
            
           
          Remarks, protocol and/or map (pdf) RDB07681.pdf

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 　2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
          Additional terms and conditions:
          The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          提供条件 1. 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。　2. 謝辞の表明を必要とする。
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          RDB07681 pGL4-phVEGFA DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pGL4-phVEGFA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07681).

          Reference section:

          Further references such as user reports and related articles (go to bottom)

          References and tips

          Featured content

          Featured content Promoter Collection (Firefly Luciferase) (English text)
          Featured content HIF-1 signaling pathway (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB07681_A4Am.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer:
          Sequence file: RDB07681_A4Ama.seq check
          >07681_07681_A4Am_pGL4-4174F_C10_07.ab1
              1 AAGGGGCCAG GAAAATTTTC TCTGGCCTAA CTGGCCGGTA CCTGAGCTCG CTAGCCTCGA
             61 GGATGGCGGG TAGGTTTGAA TCATCACGCA GGCCCTGGCC TCCACCCACC CCCACCAGCC
            121 CCCTGGCCTC AGTTCCCTGG CAACATCTGG GGTTGGGGGG GCAGCAGGAA CAAGGGCCTC
            181 TGTCTGCCCA GCTGCCTCCC CCTTTGGGTT TTGCCAGACT CCACAGTGCA TACGTGGGCT
            241 CCAACAGGTC CTCTTCCCTC CCAGTCACTG ACTAACCCCG GAACCACACA GCTTCCCGTT
            301 CTCAGCTCCA CAAACTTGGT GCCAAATTCT TCTCCCCTGG GAAGCATCCC TGGACACTTC
            361 CCAAAGGACC CCAGTCACTC CAGCCTGTTG GCTGCCGCTC ACTTTGATGT CTGCAGGCCA
            421 AATGAGGGCT CCAGATGGCA CATTGTCAGA GGGACACACT GTGGCCCCTG TGCCCAGCCC
            481 TGGGCTCTCT GTACATGAAG CAACTCCAGT CCCAAATATG TAGCTGTTTG GGAGGTCAGA
            541 AATAGGGGGT CCAGGAGCAA ACTCCCCCCA CCCCCTTTCC AAAGCCCATT CCCTCTTTAG
            601 CCAGAGCCGG GGTGTGCAGA CGGCAGTCAC TAGGGGGCGC TCGGCCACCA CAGGGAAGCT
            661 GGGTGAATGG AGCGAGCAGC GTCTTCGAGA GTGAGGACGT GTGTGTCTGT GTGGGTGAGT
          721 GAGTGTGTGC GTGTGGGGTT GAGGGTGTTG GAGCGGGCGT ATGCCCTGTG GTGA
          //
          Primer:
          Sequence file: RDB07681_A4Amb.seq check
          >07681_07681_A4Am_pGL4-136R_D10_10.ab1
              1 GGCCTAAAGT TTTTTTGGCA TCTTCCATGG TGGCTTTACC AACAGTACCG GATTGCCAAG
             61 CTTGGCCGCC GAGGCCAGAT CTTGATAGGG ATAAAACCCG GATCAATGAA TATCAAATTC
            121 CAGCACCGAG CGCCCTGGCC GGTGAGTCCG CTGACCGGTC CACCTAACCG CTGCGCCTCC
            181 CGACAGAGCG CTGGTGCTAG CCCCCAGCGC CACGACCTCC GAGCTACCCG GCTGCCCCAA
            241 GCCTCCGCGA TCCTCCCCGC TACCAGCCGA CTTTTAAAAA AAAAAGGGGG GGGCGCATGG
            301 CTCCGCCCCG CCGGGACCCC GCCCCCGGCC CGCCCCGGGG GGCGGGGACA GGCGAGCCTC
            361 AGCCCCTCCA CACGCGGCTC GGGCCCGGGC TCTGCGGACG CCCAGTGAAG CCTGGCCCGC
            421 AGCCCGCCGC AATGAAGGGG AAGCTCGACC CCCACCAAGG TTCACAGCCT GAAAATTACC
            481 CATCCGCCCC CGGAAACTCT GTCCAGAGAC ACGCGCCCGC GGGGCATTGG CGAGGAGGGA
            541 GCAGGAAAGT GAGGTTACGT GCGGACAGGG CCTGAGAGCC GTTCCCTCTT TGCTAGGAAT
            601 ATTGAAGGGG GCAGGGGAAG GCGGAGAGCC GGACAGGGAC GGGTGGGGAG AGGGACACAC
            661 AGATCTATTG GAATCCTGGA GTGACCCCTG GCCTTCTCCC CGCTCCAACA CCCTCAACCC
            721 CACACGCACA CACTCACTCA CCCACACAGA CACACACGTC CTCACTCTCG AAGACGCTGC
            781 TCGCTCCATT CACCCAGCTT CCCTGTGGTG GCCGAGCGCC CCCTAGTGAC TGCCGTCTGC
            841 ACACCCCGGC TCTGGCTAAA GAGGGAATGG GCTTTGGAAA GGGGGTGGGG GGAGTTTGCT
            901 CCTGGACCCC CTATTTCTGA CCTCCCAAAC AGCTACATAT TTGGGACTGG AGTTGCTTCA
            961 TGTACAGAGA GCCCAGGGCT GGGCACAGGG GCCACAGTGT GTCCCTCTGA CAATGTGCCA
           1021 TCTGGAGCCC TCATTTGGCC CTGCAGACAT CAAAGTGAGC GGCAGGCCAA CAAGCTGGAG
           1081 TGACTGGGTC CTTTGGGAAG TGTCCAGGAT GCTTCCCAAG GAGAGAATTG CACCAGGTTG
          1141 TTGAGCTGGA GAAACCGGGA AGGT
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          user_report Hasan, A.U., Eicosapentaenoic acid upregulates VEGF-A through both GPR120 and PPARγ mediated pathways in 3T3-L1 adipocytes. Mol. Cell. Endocrinol. 406:10-18 (2015). PMID 25697344.

          2023.05.02

          GNP_filter3_RDBDEP_html_230501.pl