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pGL4-phNQO1 (#RDB07570)

Promoter collection, Human NQO1 promoter

Alternative name U2796(2174)-3
Clone info. PCR amplified human NQO1 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 69727985 to 69726587(NC_000016.10); relatively -1355 to +44, where +1 corresponds to 1 nt of NM_000903.2.
Comment PCR cloning, forward primer: ATTTTGTCAGATGGGGTCTCAC; reverse primer: ATATCCTGTCCGGCCCGTTT.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human NAD(P)H dehydrogenase, quinone 1 genomic DNA
Depositor|Developer DNA Bank, | Kishikawa, Shotaro |
 
Remarks, protocol and/or map (pdf) RDB07570.pdf

Distribution information

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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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RDB07570 pGL4-phNQO1 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phNQO1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07570).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB07570_A2Gop1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4ins_F (Pr0665)
Region: pause site, insert 5'
Sequence file: RDB07570_A2Goa.seq check
>07570_07570_B0JgF_pGL4ins_F_F01_06_ABI08.ab1
    1 NNNNNNNNNN NNNNCNNGNN NNNNNGGCTG TCCCCAGTGC AAGTGCAGGT GCCAGAACAT
   61 TTCTCTGGCC TAACTGGCCG GTACCTGAGC TCGCTAGCCT CGAGGATATT TTGTCAGATG
  121 GGGTCTCACT TTGTTGCTCA GGCTTTTCTC AAACTCTTGG GCTCAAGTGA TCCTCCTGCC
  181 TCAGCCTCCC AAAGTAATTT GTAAATACGT GTAAATAATA TAGTCCTCTC TTGTTGCATC
  241 AAAATGAATG CAAAAATGTC ACGATGGGGC CGGGCGCGGT GGCTCATGCC TGTAATCCCA
  301 GCACTTCGGG AGGCCGAGGC GGTAGGATCA CCTGAGGTCG GGAGTTGGAA ACCAGTTTTC
  361 TGGTGCCTCC TTAACTTTTG CACTGGAGGG ACAACGGAAG GGCCCTGTGT CATCCTCACC
  421 TCCCCCATCT GTGATTCTTC TGATGGTTAA TTACCACTTT AAAGCTCTAG TTCTTTTTCC
  481 TTCACCTACT GAGAGTATAT CTCCTTCTTT ACAGTAACTT TTACTTTATA GAGTTTTTTT
  541 TGAGACGGAG TCTCACTTCG TCACCCAGGC GGGAGTGCAG TGGCACGATC TCGACCCACT
  601 GCAACCTCCG CCTTCCGGGT TCNAGCGANT CTCCTGCCTC ANCCTCCCGA GTNNCTGGGA
661 CTTANAGGCG CCCNNCNTCN CGCCNNGCTN ANTTTTNNNN NNNNNN
//
Primer: pGL4ins_R (Pr0666)
Region: Luc 5', insert 3'
Sequence file: RDB07570_A2Gob.seq check
>07570_07570_B0JgF_pGL4ins_F_F01_06_ABI08.ab1
    1 NNNNNNNNNN NNNNCNNGNN NNNNNGGCTG TCCCCAGTGC AAGTGCAGGT GCCAGAACAT
   61 TTCTCTGGCC TAACTGGCCG GTACCTGAGC TCGCTAGCCT CGAGGATATT TTGTCAGATG
  121 GGGTCTCACT TTGTTGCTCA GGCTTTTCTC AAACTCTTGG GCTCAAGTGA TCCTCCTGCC
  181 TCAGCCTCCC AAAGTAATTT GTAAATACGT GTAAATAATA TAGTCCTCTC TTGTTGCATC
  241 AAAATGAATG CAAAAATGTC ACGATGGGGC CGGGCGCGGT GGCTCATGCC TGTAATCCCA
  301 GCACTTCGGG AGGCCGAGGC GGTAGGATCA CCTGAGGTCG GGAGTTGGAA ACCAGTTTTC
  361 TGGTGCCTCC TTAACTTTTG CACTGGAGGG ACAACGGAAG GGCCCTGTGT CATCCTCACC
  421 TCCCCCATCT GTGATTCTTC TGATGGTTAA TTACCACTTT AAAGCTCTAG TTCTTTTTCC
  481 TTCACCTACT GAGAGTATAT CTCCTTCTTT ACAGTAACTT TTACTTTATA GAGTTTTTTT
  541 TGAGACGGAG TCTCACTTCG TCACCCAGGC GGGAGTGCAG TGGCACGATC TCGACCCACT
  601 GCAACCTCCG CCTTCCGGGT TCNAGCGANT CTCCTGCCTC ANCCTCCCGA GTNNCTGGGA
661 CTTANAGGCG CCCNNCNTCN CGCCNNGCTN ANTTTTNNNN NNNNNN
//
Primer: SV40pA_R (Pr0882)
Region: SV40pA, Luc 3'
Sequence file: RDB07570_A2Goc.seq check
>07570_07570_B0JgF_SV40pA_R_H01_08_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNATTT TTTTNNCTGC ATTCTAGTTG
   61 TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGC TCGAAGCGGC CGGCCGCCCC
  121 GACTCTAGAA TTATTACACG GCGATCTTGC CGCCCTTCTT GGCCTTAATG AGAATCTCGC
  181 GGATCTTGCG GGCGTCCAAC TTGCCGGTCA GTCCTTTAGG CACCTCGTCC ACGAACACAA
  241 CACCACCGCG CAGCTTCTTG GCGGTTGTAA CCTGGCTGGC CACATAGTCC ACGATCTCCT
  301 TCTCGGTCAT GGTTTTACCG TGTTCCAGCA CGACGACTGC GGCGGGCAGC TCGCCGGCAT
  361 CGTCGTCGGG CAGGCCGGCG ACCCCGGCGT CGAAGATGTT GGGGTGTTGC AGCAGGATGC
  421 TCTCCAGTTC GGCTGGGGCT ACCTGGTAGC CCTTGTATTT GATCAGGCTC TTCAGCCGGT
  481 CCACGATGAA GAAGTGCTCG TCCTCGTCCC AGTAGGCGAT GTCGCCGCTG TGCAGCCAGC
  541 CGTCCTTGTC GATGAGAGCG TTTGTAGCCT CGGGGTTGTT AACGTAGCCG CTCATGATCA
  601 TGGGGCCACG GANNNNNNNC TCGCCGCGCT NGTTCACACC CAGTGTCTTA CCGGTGTCCN
  661 NNTCCACCAC CNNANCCTCN AAGANNNGNA NCNCCTNGCC TACNGCGNCN NGGCNNGNCG
721 TCCCCNNNNN NGGGNNNNNN NNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles