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pCMFlag_hsCREB1, transcript variant B

Expression vector of human CREB1.

Catalog number RDB07505
Resource name pCMFlag_hsCREB1, transcript variant B
Alternative name SET-0328_1-1
Clone info. Expression vector of human CREB1. Expression has not yet been confirmed.
Comment PCR cloning, forward primer: ATGACCATGGAATCTGGAGCCGA; reverse primer: AATTAATCTGATTTGTGGCAGTAAA.
Vector backbone pCMV_S-FLAG (Plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human CREB1,transcript variant B cDNA
Depositor|Developer DNA Bank, |
Other clones in our bank
External Database
Human CREB1,transcript variant B
          Reference sequence
            
           

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          RDB07505 pCMFlag_hsCREB1, transcript variant B DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pCMFlag_hsCREB1, transcript variant B was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07505).

          Reference section:

          Further references such as user reports and related articles (go to bottom)

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          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB07505_A4Eep1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward (Pr0016)
          Region: CMV pro,T7 pro,FLAG, insert 5'
          Sequence file: RDB07505_A4Eec.seq check
          >07505_07505_A4Ee_CMV-Foward_C02_08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNGCNNA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT
             61 ATATAAGCAG AGCTCTCTGG CTAACTAGAG AACCCACTGC TTACTGGCTT ATCGAAATTA
            121 ATACGACTCA CTATAGGGAG ACCCAAGCTG AATTCACCAT GGACTACAAG GACGATGACG
            181 ATAAGGCGGC CATGACCATG GAATCTGGAG CCGAGAACCA GCAGAGTGGA GATGCAGCTG
            241 TAACAGAAGC TGAAAACCAA CAAATGACAG TTCAAGCCCA GCCACAGATT GCCACATTAG
            301 CCCAGGTATC TATGCCAGCA GCTCATGCAA CATCATCTGC TCCCACCGTA ACTCTAGTAC
            361 AGCTGCCCAA TGGGCAGACA GTTCAAGTCC ATGGAGTCAT TCAGGCGGCC CAGCCATCAG
            421 TTATTCAGTC TCCACAAGTC CAAACAGTTC AGTCTTCCTG TAAGGACTTA AAAAGACTTT
            481 TCTCCGGAAC ACAGATTTCA ACTATTGCAG AAAGTGAAGA TTCACAGGAG TCAGTGGATA
            541 GTGTAACTGA TTCCCAAAAG CGAAGGGAAA TTCTTTCAAG GAGGCCTTCC TACAGGAAAA
            601 TTTTGAATGA CTTATCTTCT GATGCACCAG GAGTGCCAAG GATTGAAGAA GAGAAGTCTG
            661 AAGAGGAGAC TTCAGCACCT GCCATCACCA CTGTAACGGT GCCNACTCCA ATTTACCAAA
            721 CTAGCAGTGG ACAGTATATT GCCATTACCC AGGGANGAGC AATACAGCTG GCTAACAATG
            781 GTACCGATGG GGTACAGGGC CTGCAAACAT TAACCATGAC CAATGCAGCA GCCACTCAGC
            841 CGGGTACTAC CATTCTACAG TATGCACAGA CCACTGATGG ACAGCAGATC TNANTGCCNA
            901 NCNACCAAGT TGTTGTTCNA GCTGCCTCTG GAGACGTACA AACATACNNG ANTCGCACAG
            961 CACCNACTAN CACTATTGCC NNNNGNNTNG NNNTNGNNTN CNCCCNNNCN NNNNCNNANN
          1021 NNNNCNNNNN NNANNNNNNN NNN
          //
          Primer: bGH_rev3 (Pr0885)
          Region: bGH pA,SP6 pro,insert 3'
          Sequence file: RDB07505_A4Eed.seq check
          >07505_07505_B3KhS_bGH_rev3_F01_06_ABI08.ab1
              1 NNNNNNNNNN NNNNGGNNGN NGGGCNNACA ACAGATGGCT GGCAACTAGA AGGCACAGTC
             61 GAGGCTGATC AGCGAGCTCT AGCATTTAGG TGACACTATA GAATAGGGCC CTCTAGATGC
            121 ATGCTCGAGC GGCCAATTAA TCTGATTTGT GGCAGTAAAG GTCCTTAAGT GCTTTTAGCT
            181 CCTCAATCAA TGTCTTGTTT TGATTTTCAA GCACTGCCAC TCTGTTTTCT AAACATTTCA
            241 CATATTCTTT CTTCTTTCTA CGACACTCTC GAGCTGCTTC CCTGTTCTTC ATTAGACGGA
            301 CCTCTCTCTT TCGTGCTGCT TCTTCAGCAG GCTGTGTAGG AAGTGCTGGG GAGGATGCCA
            361 TAACAACTCC AGGGGCAATA GTGCTAGTGG GTGCTGTGCG AATCTGGTAT GTTTGTACGT
            421 CTCCAGAGGC AGCTTGAACA ACAACTTGGT TGCTGGGCAC TAAGATCTGC TGTCCATCAG
            481 TGGTCTGTGC ATACTGTAGA ATGGTAGTAC CCGGCTGAGT GGCTGCTGCA TTGGTCATGG
            541 TTAATGTTTG CAGGCCCTGT ACCCCATCGG TACCATTGTT AGCCAGCTGT ATTGCTCCTC
            601 CCTGGGTAAT GGCAATATAC TGTCCACTGC TAGTTTGGTA AATTGGAGTT GGCACCGTTA
            661 CNGTGNTGAN GNCANGTGCT GANNTCTCCT CTTCNNANTT CTCTTCNTCN NNNCNNNNGN
          721 ANTCCNNNNN NNNNN
          //
          Primer: SV40pro_ori_F (Pr0731)
          Region: SV40 pro_ori,NeoR
          Sequence file: RDB07505_A4Eee.seq check
          >07505_07505_B3KhS_SV40pro_ori_F_G01_07_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN GCNGCCTCNG CCTCTNNNCT ATTCNNGAAG TAGTGAGGAG
             61 GCTTTTTTGG AGGCCTANGC TTTTGCAAAA AGCTCCCGGG AGCTTGGATA TCCATTTTCG
            121 GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG
            181 CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA
            241 TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG
            301 TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT
            361 GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA
            421 GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC
            481 CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG
            541 CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG
            601 AAGCCGGTCT TGTCGATCAN GANGATCTGG ACGAANAGCA TCNNGGGCTC GCGCCNGCCG
            661 AACTGTTCGN CAGGCTNNAN NNGCGCATGC CCNACGGCGA NGNNCTCNTC NNGACCCANG
          721 NNNNNGCNNG CTNNNNNNAN NNNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          2024.03.26

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