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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGL4-phSERPINE1(PAI-1)

Promoter collection, Human SERPINE1 promoter

Catalog number RDB07461
Resource name pGL4-phSERPINE1(PAI-1)
Alternative name U2851-5
Clone info. PCR amplified human SERPINE1 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 101125677 to 101127125(NC_000007.14); relatively -1412 to +37, where +1 corresponds to 1 nt of NM_000602.4.
Comment PCR cloning, forward primer: GCCACTCCTCATCACTCGCATT; reverse primer: CCCTGCAGCCAAACACAGC. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 genomic DNA
Depositor|Developer DNA Bank, |
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External Database
Human serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1

          Reference sequence
            

          Distribution information

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          Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          RDB07461 pGL4-phSERPINE1(PAI-1) DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (pdf) RDB07461.pdf

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Dnaconda's recommendation EXR0096e (English text)
          Featured content Dnaconda's recommendation EXR0096j (Japanese text)
          Featured content Promoter Collection (Firefly Luciferase) (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13380_A5Dn.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGL4-4174F
          Sequence file: RDB13380_A5Dna.seq check
          >07461_13380_A5Dn_pGL4-4174F_A06_03.ab1
              1 CTGGTCAGAC TTTCTCTGGC CTACTGGCCG GTACCTGAGC TCGCTAGCCT CGAGGATGCC
             61 ACTCCTCATC ACTCGCATTA CCACCAGAGC TCCACTCCCT GTCAGATCAG CGGCGGCATT
            121 AGATTCTCAT AGGAGCTCGA ACCCTATTCT AAACTGTTCA TGTGAGGGAT CTAGGTTGCA
            181 AGCTCCCTAT GAGAATCTAA TGCCTGATGA TCTGTCACGG TCTCCCATCA CCCCTAGATG
            241 GGACCATCTA GTTGCAGGAA AACAAGCTCA GGGCTCCCAC TGATTCTACA CGATGGTGAA
            301 TTGTGGAATT ATTTCATTAT ATATATTACA ATGTAATAAT AATAGAAATA AAGCACACAA
            361 TAAATGTAAT GTGCTTGAAT CATCCCGAAA CCATCCCACC CTGGTCTGTG AAAAAATTGT
            421 CTTCCATGAA ACCAGTCCCT GGTGCCAAAA ACGTTGAGGA CCACTGCTCC ACAGAATCTA
            481 TCGGTCACTC TTCCTCCCCT CACCCCCTTG CCCTAAAAGC ACACCCTGCA AACCTGCCAT
            541 GAATTGACAC TCTGTTTCTA TCCCTTTTCC CCTTGTGTCT GTGTCTGGAG GAAGAGGATA
            601 AAGGACAAGC TGCCCCAAGT CCTAGCGGGC AGCTCGAGGA AGTGAAACTT ACACGTTGGT
            661 CTCCTGTTTC CTTACCAAGC TTTTACCATG GTAACCCCTG GTCCCGTTCA GCCACCACCA
            721 CCCCACCCAG CACACCTCCA ACCTCAGCCA GACAAGGTTG TTGACACAAG AGAGCCCTCA
            781 GGGGCACAGA GAGAGTCTGG ACACGTGGGG GAGTCAGCCG TGTATCATCG GAGGCGGCCG
            841 GGCACATGGC AGGGATGAGG GAAAGACCAA GAGTCCTCTG TTGGGCCCAA GTCCTAGACA
            901 GACAAAACCT AGACAATCAC GTGGCTGGCT GCATGCCCTG TGGCTGTTGG GCTGGGCCCA
            961 GGAGGAGGGA GGGGCGCTCT TTCCTGGAGG TGGTCCAGAG CACCGGGTGG ACAGGCCCTT
           1021 GGGGAAAACC TTCACGTTTT GAATGGAAGA TAATCCTTGG ATACTCGACA GTGACCTGGC
          1081 TTCGCTAACG AAAAGGCAGG CTAACGGTTG A
          //
          Primer: pGL4-136R
          Sequence file: RDB13380_A5Dnb.seq check
          >07461_13380_A5Dn_pGL4-136R_B06_06.ab1
              1 GCTATGCTAT GGCATCTTCA TGGTGGCTTT ACCAACAGTA CCGGATTGCC AAGCTTGGCC
             61 GCCGAGGCCA GATCTTGATC CCTGCAGCCA AACACAGCTG TGCTCCTCTG TGGGCCACTG
            121 CCTCCTTTTA TACCAGATGT GGGCAGGAAA TAGATGAACT CATGTTCCAG CCCCACCCAC
            181 TCACTGGCTC TGGGAGTCCG TCTGAACAGC CAGCGGGTCC CCCTCGACAC CTCCCTCTCT
            241 GGGACTTGCT GAGGCATGTG TGTGTGTGTG TGTGTGCTGC TCTGTGTGTG TACGTGTGTA
            301 AGAGCATTCA GGAACAATTG AGCAAACCCC AATAGCCTTG GCCTGAGAAC CTCCCTTGAC
            361 CTTTCTGCCC TCTGCCTGTG TCTGTCTCTC CCGGATGTCC TGCCAGGTTG ACTGTCTGCC
            421 ATGCCGGGTG ACCCAAAAAG CCTAGGACCC CTAGTGTTCA GCTTGGAGAA AAAAAAAACA
            481 GCTCACGTTG CCTGCTTTTC CTTTGGCGAA CCAGGTCACT GTGGAGTTAT CAAAGATAAC
            541 CTCCATCAAA ACGTGGAAGT TTTCCCCCAG GGCTGTCCAC CCGGTGCTCT GGACCACCTC
            601 CAGGAAAGAG CGCCCCTCCC TCCTCCTGGG CCCAGCCCAA CAGCCACAGG GCATGCAGCC
            661 AGCCACGTGA TTGTCTAGGT TTTGTCTGTC TAGGACTTGG GCCCAACAGA GGACTCTTGG
            721 TCTTTCCCTC ATCCCTGCCA TGTGCCCGGC CGCCTCCGAT GATACACGGC TGACTCCCCC
            781 ACGTGTCCAG ACTCTCTCTG TGCCCCTGAG GGCTCTCTTG TGTCAACAAC CTTGTCTGGC
            841 TGAGGTTGGA GGTGTGCTGG TGGGGTTTGT GTGGGGGGGG TCTTGAAACG GGAAACCCAA
            901 GGGGGTATAC CCATGGTAAA AAGCCTTTGG GTTAAGGGAA AACAGGGAGA CCCAAACGTG
            961 TAAAGTTTTC ACTTTCCCTC GAGCTGCCCC GCTAGGACTT TGGGGGCAGC TTTGTTCCCT
           1021 TTTATCCCTC TTTCCCTCCA GACACAGACA CAAAGGGGAA AAAGGCATAG AAACAGAGTG
           1081 GTCCAATTCC ATGGCCAAGT TTGCCATGGT GTGCTTTTAC GGCCAGCGTG AAGGGAGAGA
          1141 GGACCGATAG GTAATTCG
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          user_report Resmini, G., HMGA1 regulates the Plasminogen activation system in the secretome of breast cancer cells. Sci. Rep. 7 (1): 11768 (2017). PMID 28924209.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl