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pGL4-phRHOA (#RDB07404)

Promoter collection, Human RHOA promoter

Alternative name U2765(2143)-10
Clone info. PCR amplified human RHOA promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 49413224 to 49411909(NC_000003.12); relatively -1127 to +189, where +1 corresponds to 1 nt of NM_001664.4.
Comment PCR cloning, forward primer: CAAAGGCCATTAAGCTGGTG; reverse primer: ACCAGACCGTGGACTAACGA. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human ras homolog gene family, member A genomic DNA
Depositor|Developer DNA Bank, |
 
Remarks, protocol and/or map (pdf) RDB07404.pdf

Distribution information

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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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RDB07404 pGL4-phRHOA DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phRHOA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07404).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB07404_A4D2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer:
Sequence file: RDB07404_A4D2a.seq check
>07404_07404_A4D2_1_pGL4-4174F_A12_03.ab1
    1 TAAGGGGGTC CGGACATTTT CTCTGGCCTA ACTGGCCGGT ACCTGAGCCC GCTAGCCTCG
   61 AGGATCAAAG GCCATTACGC TGGTGGGCTT AAAGCCTGAG AACTCCCCTC TCTGTCACCA
  121 GCACAGGAGT GTCCTTGGAA TGCCAAGCAT GGGGTTGCGG TATGGACAGA TTTACTCACC
  181 ACGAAGGGAA ATGCGTGGAG CCATCAGGCG TGGATCCATT CTGACCACCC AGACCTGGAG
  241 GCAGAAACAC ATCCAGAGCT GCAGAAGGCT GGGATGGGAA TCTGAGCAGG CATCAGCCCA
  301 GGTCAGGTTA GAGACCACTG GTATTGTTTG GTGAAGTCTG GTGAGGGTCC TAAGGGACTA
  361 ATGGTATTGC CAGGTGAGAG TTCTGAGGAA CAGATACAAT GTGATGGGTG GACTGGTGGG
  421 GATTCTGGCG TAAAATGGTA TGGTGGCATG AGTCAGGGCA CAACGTTAAG TTCTCCCATC
  481 CAAGTACCTG GCTTAGCTTC CGAGGTCAGA TGCGTTCAGG GTAGTAAGGC CGTGAACCAA
  541 TGTTAAGTTC TGATGAGCCT CTTGCAGGAT AGTGGGGAAC CCAAATGGTA CAGTCGGGAG
  601 AGGGTGGGGG CGGGGGCGGG CCAGCCCACG GAGGTTCGTA GAAGCCTCAC CTGGACGGTG
  661 ATACAACCCG GTCACTGTAT TCCCGTAGAA CCCCCACGCC AGCTGGATGC CCAGGAGACT
  721 TAACACCACC ACAGCAGCAG CAGCTTGAGA GGTCACGCGC ATGTCCTGCG CCTCCCAACT
  781 CCCGCAAGAA CTCGCTGCCC AGCGCGCCCA GCACCGTGGC GCAAGAGCTG CAGCTTGCCC
  841 AGACAGACTC CGCCATGAAC TACCCTCGCC CACCGGGTAC TGTCACCGTA TCCACCACGC
  901 ACGCGTGTGT GCTTCGGGAA CAGGTCGTAT GGCCCACGAT ACCAGCCAGG TGGGCCTAAC
  961 TTCCGTGCGT GAAAGATTGG CATTCCGGGG AGAGATATAA ACGCAAACGC CATACCACAC
1021 CTGCTGCTTA GGGATAGTAT TCGGGATAAA TAGCAG
//
Primer:
Sequence file: RDB07404_A4D2b.seq check
>07404_07404_A4D2_1_pGL4-136R_D12_12.ab1
    1 GGGCCCTTGT TTTTTTGGGC ATCTTCCATG GTGGCTTTAC CAACAGTACC GGATTGCCAA
   61 GCTTGGCCGC CGAGGCCAGA TCTTGATACC AGACCGTGGA CTAACGAGAG AACCGACGGA
  121 GGACCGCGGG CGGCGGGAGG GTAGCGCGAG AGAGCGAGGG CGGGCGGCGG CGGGAGGGCG
  181 GGGAACGACG TGCGCGCCTC CGAGTGCCCA GCGGGCTCGA GCCGGCACAG GTCGCGGCCC
  241 CGCGCACGCG CGCGCACTCA CAGCTCATCC ACTATTGCTC AGGAGCCCGT CCACGCCCTA
  301 AAAGCAAAAC CCTTAACCAA TCATTGCAGC CGCGTGGGGC ATCACGGGAA CTCCGGGGCT
  361 ATAAAAGACC TGGTTGCTAT TTTATACTCC CGGAGTCACT CATCCCTTAA GCAAGCAGGG
  421 TGGGGTTAGG TGCGCGTGCG CGGTTTTAAT ACTCCTCCCC GAACTGCCAA CTCTTCACGC
  481 ACGCGAAGTA GGCCCCACCC TGGCTGGGTT TACGCGTGCG CACTAACGGG CCTGGTCCCG
  541 GAAGACCACA CGCGTGCGTG GTGGGGACTA CGGTGACAGT ACCCCGGGTG GGGCGAGGGC
  601 CAGTCATGGC GGAGTCCTGG TCTGGGCAGG CCTTGCAGGC TCTGCCGGCC ACGGTGCTGG
  661 GCGCGCTGGG CAGCGAGTTC TTGCGGGAGT GGGAGGCGCA GGACATGCGC GTGACCCTCT
  721 TCAAGCTGCT GCTGCTGTGG TTGGTGTTAA GTCTCCTGGG CATCCAGCTG GCGTGGGGGT
  781 TCTACGGGAA TACAGTGACC GGGTTGTATC ACCGTCCAGG TGAGGCTTCC TACGAACCTC
  841 CGTGGGCTGG CCGCCCCCGC CCCCACCCTC TCCCGACTGT ACCATTGGGT TCCCCACTAT
  901 CCTGCAAGAG GCTCATCAGA ACTTAACATT GGGTTCACGG CCTTACTACC CTGAACGCAT
  961 CTGACCTCGG AAGCTAAGCC AGGTACTTGG ATGGGAGAAC TTAACGTTGT GCCCTGACTC
 1021 ATGCCCACCA TACCATTTTT ACGCCCAGAA TCCCCACCAA GTCCCACCCC ATCACATTTG
 1081 TATCTGTTCC TCAAGAACTC TCAACTGCAT TACAATTTAA GTTCCTTAAG GGAACCCCTC
1141 CACCCAGAAA CTTTCCCACC CAAGGA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles