Promoter collection, Human EGR1 promoter
Alternative name | U2714(2092)-40 |
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Clone info. | PCR amplified human EGR1 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 138464340 to 138465649(NC_000005.10); relatively -1152 to +158, where +1 corresponds to 1 nt of NM_001964.3. |
Comment | PCR cloning, forward primer: CATAGGGAAGCCCCTCTTTC; reverse primer: GAACACTGAGAAGCGTGCAG. Entire sequence of promoter region has not been confirmed. |
Vector backbone | pGL4.10 [Luc2] (Plasmid) |
Selectable markers | Amp^r |
Gene/insert name | Human early growth response 1 genomic DNA |
Depositor|Developer | DNA Bank, | |
Remarks, protocol and/or map (pdf) | RDB07369.pdf |
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Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation. |
必要書類 | 提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 謝辞の表明を必要とする。 MTAに書く付加的使用条件: 本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB07369 | pGL4-phEGR1 | DNA solution |
Materials & Methods section:
The pGL4-phEGR1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07369). |
Reference section:
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB07369_A3I5.pdf |
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Nucleotide sequence of a portion of this resource (if available).
Primer: pGL4-4174F Sequence file: RDB07369_A3I5a.seq |
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>07369_07369_A3I5_pGL4-4174F_G04_19.ab1 1 AACCAGGGCC AGAACATTTC TCTGGCCTAA CTGGCCGGTA CCTGAGCTCG CTAGCCTCGA 61 GGATCATAGG GAAGCCCCTC TTTCGGATTC CCGCAGTGTG GGCCGGCCCT CCACCTGGAC 121 TGGATAAAGG GGGGAAAGTG ACCCCTCACC ACAAGGACCA TTATCTCCTG GTGAGAACAA 181 GAATCAGGCC TCTCTTGGGG CAATCAGCTT CCCCACTTCG GTCCCCCAAA GGTGGGCTCT 241 TTGCCGGCGG GGACTAGGGA ACAGCCTTTC GGTTCCGGGG GAGCACAGGG GACCCCAGGC 301 ACCAGCAGCC CCATCCCACC GACAGGTGGC AGAGGCAAGG CAGCTCACTG CTATACAGTG 361 TCCCAAGAAC CAAGTGGCCG TGACTTCCTA TCCTCAATTT CCCAGCGACA CCCGGAAAGA 421 CACCGTGCCA TAGATCGAGG CCCGGGGTCA AGGCCCCGCC TCTCCTGGGC GGCCCCTGCC 481 CAGGCGGGCC CAGCCGCTCC TCCCCCGCAC TCCCGGTTCG CTCTCACGGT CCCTGAGGTG 541 GGCGGGCGGG CCCTGGATGA CAGCGATAGA ACCCCGGCCC GACTCGCCCT CGCCCCCGCT 601 CTGGGTCTGG GCTTCCCCAG CCTAGTTCAC GCCTAGGAGC CGCCTGAGCA GCCGCGCGCC 661 CAGCGCCACA CGCCACGAGC CCTCCCCGCC TGGGCGTCCC CGGATCCCGC GAGCGCTCGG 721 GCTCCCGGCT TGGAACCAGG GAGGAGGGAG GGAGCGAGGG AGCAACCAGC TGCGACCCGG 781 AAATGCCATA TAAGGAGCAG GAAGGATCCC CCGCCGGAAC AACCCTTATT TGGGCAGCAC 841 CTTATTTGGA GTGGCCCGAT ATGGCCCGGC CGCTTCCGGC TCTGGGAGGA GGGAAGAAGG 901 CGGAGGGAGG GGCAACGCGG GAACTCCGGA GCTGCGCGGG TCCCGGGAGG CCCCGGCGGC 961 GGCTAGAGCT CTAGGCTTCC CCGAAGCCCT GGCGCCTGGA TGCGGGCGCG GCGCGGCCCT 1021 AAGGTGCAGA TGGAGTGCCC GGCGCTGTCG ATGGGGGGCT TCACGTCACT CCGGTCTCCC 1081 GACCGTCTGC CCATATAGCT CTGCTCCATA TATGACATGT TACGTCACGA CGGAGCCGGA 1141 CCCGT // |
Primer: pGL4-136R Sequence file: RDB07369_A3I5b.seq |
>07369_07369_A3I5_pGL4-136R_H04_22.ab1 1 TCCCGTCTGG GAATTGTTTT TGGCATCTTC CATGGTGGCT TTACCAACAG TACCGGATTG 61 CCAAGCTTGG CCGCCGAGGC CAGATCTTGA TGAACACTGA GAAGCGTGCA GGCGGCGACC 121 CCGACTCGCC CTGGGCGCGG GCGGGCGTGG ACGCGCCGCG GCTGCGGAGG CTGGGGCAGG 181 GGCCGGTCCT GCGGCGGCGG AAGCTGGCTG CGGCGGCGGA TGGCGGCGGC GGCTCCCCAA 241 GTTCTGCGCG CTGGGATCTC TCGCGACTCC CCGGATCCGC CTCTATTTGA AGGGTCTGGA 301 ACGGCACGGG TCCGCCTCCG TCGTGACGTA CATGGCCATA TATGGGAAGC AGGAAGCCCT 361 AATATGGCAG GACCGGCCGG GAGGACCCGG AGTGACGTGA AGCCCCCCAT CCGACAGCGC 421 CCGGCACCTC CATCCTGCAC CCTAGGGCCC GCGCCCGCGC CCGCATCCCA GGCGCCCAGG 481 CTTCGGGGAA GCCTAGAGCT CTAGCCGCCG CCGGGGCCTC CGGGACCCGC GCAGCTCCGG 541 AGTTCCCGCG TTGCCCCTCC CTCCGCCTTC TTCCCTCCTC CCAGAGCCGG AAGCGGCCGG 601 GCCATATCGG GCCACTCCAA ATAAGGTGCT GCCCAAATAA GGGTTGTTCC GGCGGGGGAT 661 CCTTCCTGCT CCTTATATGG CATTTCCGGG TCGCAGCTGG TTGCTCCCTC GCTCCCTCCC 721 TCCTCCCTGG TTCCAAGCCG GGAGCCCGAG CGCTCGCGGG ATCCGGGGAC GCCCAGGCGG 781 GGAGGGCTCG TGGCGTGTGG CGCTGGGCGC GCGGCTGCTC AGGCGGCTCC TAGGCGTGAA 841 CTAGGCTGGG GAAGCCCAGA CCCAGAGCGG GGGCGAGGGC GAGTCGGGCC GGGGTTTCTA 901 TCGCTGTTCA TCCAGGGCCC GCCCGCCCAC CTCAGGGACC GTGAGAGCGA ACCCGGGAGT 961 GCGGGGGGAG GAGCGGCTGG GCCCGCCTGG GCAGGGGCCC GCCCAAGGAG AGGCGGGGCC 1021 TTGACCCCGG GTCTTCGATC TATGGGCACG GTGTCTTTCC GGTGTCCGCT GGAAAATTGA 1081 GGATAGGAGT TCACCGGCCA CTTGTTTCCT GGGAACACTT GTAATAAGCA TGAAGCCTGG 1141 CTTGGCTTTC TGCACTGCTC TGTTGGAATT GAGGACACGT // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
user_report | Suzuki, T., Arsenite-induced histone H3 modification and its effects on EGR1 and FOS expression in HeLa cells. J. Appl. Toxicol. 38 (5): 734-743 (2018) PMID 29350772. [link to RRC of NBRP] |
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