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pGL4-phEGR1 (#RDB07369)

Promoter collection, Human EGR1 promoter

Alternative name U2714(2092)-40
Clone info. PCR amplified human EGR1 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 138464340 to 138465649(NC_000005.10); relatively -1152 to +158, where +1 corresponds to 1 nt of NM_001964.3.
Comment PCR cloning, forward primer: CATAGGGAAGCCCCTCTTTC; reverse primer: GAACACTGAGAAGCGTGCAG. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human early growth response 1 genomic DNA
Depositor|Developer DNA Bank, |
 
Remarks, protocol and/or map (pdf) RDB07369.pdf

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RDB07369 pGL4-phEGR1 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phEGR1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07369).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB07369_A3I5.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F
Sequence file: RDB07369_A3I5a.seq check
>07369_07369_A3I5_pGL4-4174F_G04_19.ab1
    1 AACCAGGGCC AGAACATTTC TCTGGCCTAA CTGGCCGGTA CCTGAGCTCG CTAGCCTCGA
   61 GGATCATAGG GAAGCCCCTC TTTCGGATTC CCGCAGTGTG GGCCGGCCCT CCACCTGGAC
  121 TGGATAAAGG GGGGAAAGTG ACCCCTCACC ACAAGGACCA TTATCTCCTG GTGAGAACAA
  181 GAATCAGGCC TCTCTTGGGG CAATCAGCTT CCCCACTTCG GTCCCCCAAA GGTGGGCTCT
  241 TTGCCGGCGG GGACTAGGGA ACAGCCTTTC GGTTCCGGGG GAGCACAGGG GACCCCAGGC
  301 ACCAGCAGCC CCATCCCACC GACAGGTGGC AGAGGCAAGG CAGCTCACTG CTATACAGTG
  361 TCCCAAGAAC CAAGTGGCCG TGACTTCCTA TCCTCAATTT CCCAGCGACA CCCGGAAAGA
  421 CACCGTGCCA TAGATCGAGG CCCGGGGTCA AGGCCCCGCC TCTCCTGGGC GGCCCCTGCC
  481 CAGGCGGGCC CAGCCGCTCC TCCCCCGCAC TCCCGGTTCG CTCTCACGGT CCCTGAGGTG
  541 GGCGGGCGGG CCCTGGATGA CAGCGATAGA ACCCCGGCCC GACTCGCCCT CGCCCCCGCT
  601 CTGGGTCTGG GCTTCCCCAG CCTAGTTCAC GCCTAGGAGC CGCCTGAGCA GCCGCGCGCC
  661 CAGCGCCACA CGCCACGAGC CCTCCCCGCC TGGGCGTCCC CGGATCCCGC GAGCGCTCGG
  721 GCTCCCGGCT TGGAACCAGG GAGGAGGGAG GGAGCGAGGG AGCAACCAGC TGCGACCCGG
  781 AAATGCCATA TAAGGAGCAG GAAGGATCCC CCGCCGGAAC AACCCTTATT TGGGCAGCAC
  841 CTTATTTGGA GTGGCCCGAT ATGGCCCGGC CGCTTCCGGC TCTGGGAGGA GGGAAGAAGG
  901 CGGAGGGAGG GGCAACGCGG GAACTCCGGA GCTGCGCGGG TCCCGGGAGG CCCCGGCGGC
  961 GGCTAGAGCT CTAGGCTTCC CCGAAGCCCT GGCGCCTGGA TGCGGGCGCG GCGCGGCCCT
 1021 AAGGTGCAGA TGGAGTGCCC GGCGCTGTCG ATGGGGGGCT TCACGTCACT CCGGTCTCCC
 1081 GACCGTCTGC CCATATAGCT CTGCTCCATA TATGACATGT TACGTCACGA CGGAGCCGGA
1141 CCCGT
//
Primer: pGL4-136R
Sequence file: RDB07369_A3I5b.seq check
>07369_07369_A3I5_pGL4-136R_H04_22.ab1
    1 TCCCGTCTGG GAATTGTTTT TGGCATCTTC CATGGTGGCT TTACCAACAG TACCGGATTG
   61 CCAAGCTTGG CCGCCGAGGC CAGATCTTGA TGAACACTGA GAAGCGTGCA GGCGGCGACC
  121 CCGACTCGCC CTGGGCGCGG GCGGGCGTGG ACGCGCCGCG GCTGCGGAGG CTGGGGCAGG
  181 GGCCGGTCCT GCGGCGGCGG AAGCTGGCTG CGGCGGCGGA TGGCGGCGGC GGCTCCCCAA
  241 GTTCTGCGCG CTGGGATCTC TCGCGACTCC CCGGATCCGC CTCTATTTGA AGGGTCTGGA
  301 ACGGCACGGG TCCGCCTCCG TCGTGACGTA CATGGCCATA TATGGGAAGC AGGAAGCCCT
  361 AATATGGCAG GACCGGCCGG GAGGACCCGG AGTGACGTGA AGCCCCCCAT CCGACAGCGC
  421 CCGGCACCTC CATCCTGCAC CCTAGGGCCC GCGCCCGCGC CCGCATCCCA GGCGCCCAGG
  481 CTTCGGGGAA GCCTAGAGCT CTAGCCGCCG CCGGGGCCTC CGGGACCCGC GCAGCTCCGG
  541 AGTTCCCGCG TTGCCCCTCC CTCCGCCTTC TTCCCTCCTC CCAGAGCCGG AAGCGGCCGG
  601 GCCATATCGG GCCACTCCAA ATAAGGTGCT GCCCAAATAA GGGTTGTTCC GGCGGGGGAT
  661 CCTTCCTGCT CCTTATATGG CATTTCCGGG TCGCAGCTGG TTGCTCCCTC GCTCCCTCCC
  721 TCCTCCCTGG TTCCAAGCCG GGAGCCCGAG CGCTCGCGGG ATCCGGGGAC GCCCAGGCGG
  781 GGAGGGCTCG TGGCGTGTGG CGCTGGGCGC GCGGCTGCTC AGGCGGCTCC TAGGCGTGAA
  841 CTAGGCTGGG GAAGCCCAGA CCCAGAGCGG GGGCGAGGGC GAGTCGGGCC GGGGTTTCTA
  901 TCGCTGTTCA TCCAGGGCCC GCCCGCCCAC CTCAGGGACC GTGAGAGCGA ACCCGGGAGT
  961 GCGGGGGGAG GAGCGGCTGG GCCCGCCTGG GCAGGGGCCC GCCCAAGGAG AGGCGGGGCC
 1021 TTGACCCCGG GTCTTCGATC TATGGGCACG GTGTCTTTCC GGTGTCCGCT GGAAAATTGA
 1081 GGATAGGAGT TCACCGGCCA CTTGTTTCCT GGGAACACTT GTAATAAGCA TGAAGCCTGG
1141 CTTGGCTTTC TGCACTGCTC TGTTGGAATT GAGGACACGT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

user_report Suzuki, T., Arsenite-induced histone H3 modification and its effects on EGR1 and FOS expression in HeLa cells. J. Appl. Toxicol. 38 (5): 734-743 (2018) PMID 29350772. [link to RRC of NBRP]