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pGL4-phEGR1

Promoter collection, Human EGR1 promoter

Catalog number RDB07369
Resource name pGL4-phEGR1
Alternative name U2714(2092)-40
Clone info. PCR amplified human EGR1 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 138464340 to 138465649(NC_000005.10); relatively -1152 to +158, where +1 corresponds to 1 nt of NM_001964.3.
Comment PCR cloning, forward primer: CATAGGGAAGCCCCTCTTTC; reverse primer: GAACACTGAGAAGCGTGCAG. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human early growth response 1 genomic DNA
Depositor|Developer DNA Bank, |
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External Database
Human early growth response 1

          Reference sequence
            

          Distribution information

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          Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          RDB07369 pGL4-phEGR1 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (pdf) RDB07369.pdf

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Promoter Collection (Firefly Luciferase) (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB07369_A3I5.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGL4-4174F
          Sequence file: RDB07369_A3I5a.seq check
          >07369_07369_A3I5_pGL4-4174F_G04_19.ab1
              1 AACCAGGGCC AGAACATTTC TCTGGCCTAA CTGGCCGGTA CCTGAGCTCG CTAGCCTCGA
             61 GGATCATAGG GAAGCCCCTC TTTCGGATTC CCGCAGTGTG GGCCGGCCCT CCACCTGGAC
            121 TGGATAAAGG GGGGAAAGTG ACCCCTCACC ACAAGGACCA TTATCTCCTG GTGAGAACAA
            181 GAATCAGGCC TCTCTTGGGG CAATCAGCTT CCCCACTTCG GTCCCCCAAA GGTGGGCTCT
            241 TTGCCGGCGG GGACTAGGGA ACAGCCTTTC GGTTCCGGGG GAGCACAGGG GACCCCAGGC
            301 ACCAGCAGCC CCATCCCACC GACAGGTGGC AGAGGCAAGG CAGCTCACTG CTATACAGTG
            361 TCCCAAGAAC CAAGTGGCCG TGACTTCCTA TCCTCAATTT CCCAGCGACA CCCGGAAAGA
            421 CACCGTGCCA TAGATCGAGG CCCGGGGTCA AGGCCCCGCC TCTCCTGGGC GGCCCCTGCC
            481 CAGGCGGGCC CAGCCGCTCC TCCCCCGCAC TCCCGGTTCG CTCTCACGGT CCCTGAGGTG
            541 GGCGGGCGGG CCCTGGATGA CAGCGATAGA ACCCCGGCCC GACTCGCCCT CGCCCCCGCT
            601 CTGGGTCTGG GCTTCCCCAG CCTAGTTCAC GCCTAGGAGC CGCCTGAGCA GCCGCGCGCC
            661 CAGCGCCACA CGCCACGAGC CCTCCCCGCC TGGGCGTCCC CGGATCCCGC GAGCGCTCGG
            721 GCTCCCGGCT TGGAACCAGG GAGGAGGGAG GGAGCGAGGG AGCAACCAGC TGCGACCCGG
            781 AAATGCCATA TAAGGAGCAG GAAGGATCCC CCGCCGGAAC AACCCTTATT TGGGCAGCAC
            841 CTTATTTGGA GTGGCCCGAT ATGGCCCGGC CGCTTCCGGC TCTGGGAGGA GGGAAGAAGG
            901 CGGAGGGAGG GGCAACGCGG GAACTCCGGA GCTGCGCGGG TCCCGGGAGG CCCCGGCGGC
            961 GGCTAGAGCT CTAGGCTTCC CCGAAGCCCT GGCGCCTGGA TGCGGGCGCG GCGCGGCCCT
           1021 AAGGTGCAGA TGGAGTGCCC GGCGCTGTCG ATGGGGGGCT TCACGTCACT CCGGTCTCCC
           1081 GACCGTCTGC CCATATAGCT CTGCTCCATA TATGACATGT TACGTCACGA CGGAGCCGGA
          1141 CCCGT
          //
          Primer: pGL4-136R
          Sequence file: RDB07369_A3I5b.seq check
          >07369_07369_A3I5_pGL4-136R_H04_22.ab1
              1 TCCCGTCTGG GAATTGTTTT TGGCATCTTC CATGGTGGCT TTACCAACAG TACCGGATTG
             61 CCAAGCTTGG CCGCCGAGGC CAGATCTTGA TGAACACTGA GAAGCGTGCA GGCGGCGACC
            121 CCGACTCGCC CTGGGCGCGG GCGGGCGTGG ACGCGCCGCG GCTGCGGAGG CTGGGGCAGG
            181 GGCCGGTCCT GCGGCGGCGG AAGCTGGCTG CGGCGGCGGA TGGCGGCGGC GGCTCCCCAA
            241 GTTCTGCGCG CTGGGATCTC TCGCGACTCC CCGGATCCGC CTCTATTTGA AGGGTCTGGA
            301 ACGGCACGGG TCCGCCTCCG TCGTGACGTA CATGGCCATA TATGGGAAGC AGGAAGCCCT
            361 AATATGGCAG GACCGGCCGG GAGGACCCGG AGTGACGTGA AGCCCCCCAT CCGACAGCGC
            421 CCGGCACCTC CATCCTGCAC CCTAGGGCCC GCGCCCGCGC CCGCATCCCA GGCGCCCAGG
            481 CTTCGGGGAA GCCTAGAGCT CTAGCCGCCG CCGGGGCCTC CGGGACCCGC GCAGCTCCGG
            541 AGTTCCCGCG TTGCCCCTCC CTCCGCCTTC TTCCCTCCTC CCAGAGCCGG AAGCGGCCGG
            601 GCCATATCGG GCCACTCCAA ATAAGGTGCT GCCCAAATAA GGGTTGTTCC GGCGGGGGAT
            661 CCTTCCTGCT CCTTATATGG CATTTCCGGG TCGCAGCTGG TTGCTCCCTC GCTCCCTCCC
            721 TCCTCCCTGG TTCCAAGCCG GGAGCCCGAG CGCTCGCGGG ATCCGGGGAC GCCCAGGCGG
            781 GGAGGGCTCG TGGCGTGTGG CGCTGGGCGC GCGGCTGCTC AGGCGGCTCC TAGGCGTGAA
            841 CTAGGCTGGG GAAGCCCAGA CCCAGAGCGG GGGCGAGGGC GAGTCGGGCC GGGGTTTCTA
            901 TCGCTGTTCA TCCAGGGCCC GCCCGCCCAC CTCAGGGACC GTGAGAGCGA ACCCGGGAGT
            961 GCGGGGGGAG GAGCGGCTGG GCCCGCCTGG GCAGGGGCCC GCCCAAGGAG AGGCGGGGCC
           1021 TTGACCCCGG GTCTTCGATC TATGGGCACG GTGTCTTTCC GGTGTCCGCT GGAAAATTGA
           1081 GGATAGGAGT TCACCGGCCA CTTGTTTCCT GGGAACACTT GTAATAAGCA TGAAGCCTGG
          1141 CTTGGCTTTC TGCACTGCTC TGTTGGAATT GAGGACACGT
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          user_report Suzuki, T., Arsenite-induced histone H3 modification and its effects on EGR1 and FOS expression in HeLa cells. J. Appl. Toxicol. 38 (5): 734-743 (2018) PMID 29350772.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl