Promoter collection, Human FOS promoter
Alternative name | U2638 (2016)-3 |
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Clone info. | PCR amplified human FOS promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 75277609 to 75278869(NC_000014.9); relatively -1169 to +92, where +1 corresponds to 1 nt of NM_005252.4. |
Comment | PCR cloning, forward primer: tcccagcagtcgaggtattc; reverse primer: ggctcagtcttggcttctca. Entire sequence of promoter region has not been confirmed. |
Vector backbone | pGL4.10 [Luc2] (Plasmid) |
Selectable markers | Amp^r |
Gene/insert name | Human v-fos FBJ murine osteosarcoma viral oncogene homolog genomic DNA |
Depositor|Developer | DNA Bank, | Pan, Jianzhi | |
Remarks, protocol and/or map (pdf) | RDB07292.pdf |
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Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation. |
必要書類 | 提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 謝辞の表明を必要とする。 MTAに書く付加的使用条件: 本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB07292 | pGL4-phFOS | DNA solution |
Materials & Methods section:
The pGL4-phFOS was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07292). |
Reference section:
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB07292_A3I5p1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
Primer: pGL4-4174F Sequence file: RDB07292_A3I5a.seq |
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>07292_07292_A3I5_pGL4-4174F_A04_01.ab1 1 AACTAGGGGC AGACATTTCT CTGGCCTAAC TGGCCGGTAC CTGAGCTCGC TAGCCTCGAG 61 GATTCCCAGC AGTCGAGGTA TTCCGCCCAG GCGCAGCTGG ACACTGTCCT TCCAGCCCCC 121 GTCCTCCACC CTCCAAGTCC GCGCTGGAAA ATCACCCGCT GCGGGCTCCC GTAAGCACAG 181 CTTCCTGGCG GGACCGAACC AGCCCTCAGC GCAGATTTGA GTTCCCCGCA GGAAGCACAC 241 CCCGCCTTGT CATCCCGAAC TGACCACCCT GCCCACATAA CCACACCTCG CACTCCCTAC 301 CCCTGGGGCC CAGCTCAGAA CCGGGCAGAC ACCCCCTTCA AATGTCTTCG CACGTAGGTT 361 TTGCACAGTG TTTATCTGCT GGTGTCTCAG GGATTTGACA GTTTCCTTAA TATTCCCACA 421 CATGGCCGAG AAAAATAAAT AAATAAATGC GCTGTCTTCT TTAAAAAAAT AAATAAATAA 481 AGTACCCAGT ATCGTAAAGT AGGTTATCGT ATTCTCTTAT TTTGGATCCT CCACTTTCTG 541 CTTCCAAACG CAGGAACAGT GCTAGTATTG CTCGAGCCCG AGGGCTGGAG GTTAGGGGAT 601 GAAGGTCTGC TTCCACGCTT TGCACTGAAT TAGGGCTAGA ATTGGGGATG GGGGTAGGGG 661 CGCATTCCTT CGGGAGCCGA GGCTTAAGTC CTCGGGGTCC TGTACTCGAT GCCGTTTCTC 721 CTATCTCTGA GCCTCAGAAC TGTCTTCAGT TTCCGTACAA GGGTAAAAAG GCGCTCTCTG 781 CCCCATCCCC CCCGACCTCG GGAACAAGGG TCCGCATTGA ACCAGGTGCG AATGTTCTCT 841 CTCATTCTGC GCCGTTCCCG CCTCCCCTCC CCCAGCCGCG GCCCCCGCCT CCCCCCGCAC 901 TGCACCCTCG GTGTTGGCTG CAGCCCGCGA GCAGTTCCCG TCAATCCCTC CCCCCTTACA 961 CAGGATGTCC ATATTAGGAC ATCTGCGTCA GCAGGTTTCC ACGGGCCTTT CCCTGTAGCC 1021 CTGGGGGGGA GCCATCCCCG AAACCCCTCA TCTTGGGGGG GCCCACGAGA CCTCTGAGAC 1081 AGGACTGCGA AATGCTCACG GAGGATTAGG ACACGCGCTA AGCGGGGGCA TTAAGCCTGC 1141 GAGCGCCTGG GGGACCGCCA GTCCGGGCGG ATCGGTCA // |
Primer: pGL4-136R Sequence file: RDB07292_A3I5b.seq |
>07292_07292_A3I5_pGL4-136R_B04_04.ab1 1 GCATTTTTGT TTTTGGCATC TTCCATGGTG GCTTTACCAA CAGTACCGGA TTGCCAAGCT 61 TGGCCGCCGA GGCCAGATCT TGATGGCTCA GTCTTGGCTT CTCAGATGCT CGCTGCAGAT 121 GCGGTTGGAG TACGAGGCGC CGCAGCCACT GCTTTTATAA CAAGCGTTTT ATGAATGAGT 181 GTAAACGTCA CGGGCTCAAC CACGGTGGCG CCAGAGGGGT GGCGCGCGGG CCTGGGCGCT 241 TCTGCGGCCG CCCGGCTGCG TCCCCAGCGC TCGCAGCTCC CTGCCCCCGC CTTGGCGCGT 301 GTCCTAATCT CGTGAGCATT TCGCAGTTCC TGTCTCAGAG GTCTCGTGGG CCCCCCAAGA 361 TGAGGGGTTT CGGGGATGGC TCCCCCCAGG GCTACAGGGA AAGGCCGTGG AAACCTGCTG 421 ACGCAGATGT CCTAATATGG ACATCCTGTG TAAGGGGGGA GGGATTGACG GGAACTGCTC 481 GCGGGCTGCA GCCAACACCG AGGGTGCAGT GCGGGGGGAG GCGGGGGCCG CGGCTGGGGG 541 AGGGGAGGCG GGAACGGCGC AGAATGAGAG AGAACATTCG CACCTGGTTC AATGCGGACC 601 CTTGTTCCCG AGGTCGGGGG GGATGGGGCA GAGAGCGCCT TTTTACCCTT GTACGGAAAC 661 TGAAGACAGT TCTGAGGCTC AGAGATAGGA GAAACGGCAT CGAGTACAGG ACCCCGAGGA 721 CTTAAGCCTC GGCTCCCGAA GGAATGCGCC CCTACCCCCA TCCCCAATTC TAGCCCTAAT 781 TCAGTGCAAA GCGTGGAAGC AGACCTTCAT CCCCTAACCT CCAGCCCTCG GGCTCGAGCA 841 ATACTAGCAC TGTTCCTGCG TTTGGAAGCA GAAAGTGGAG GATCCAAAAT AAGAGAATAC 901 GATAACCTAC TTTACGATAC TGGGTACTTT ATTTATTTAT TTTTTTTAAA GAAGACAGCG 961 CATTTATTTA TTTATTTTTT CTCGGGCCAT GTGTGGGAAA TATTAAAGGA AACTGTCAAA 1021 TCCTGAGACA CCAGCAGATA AACACTGTGC AAAACCTACG TGCGAAGACA TTTGAAGGGG 1081 GTGTCTGCCG GATCTGAGCT GGGACCCCAA GGGCTAAGCA GTGCGAAGTG GTGGTTATGT 1141 GGGCAGGATG GTCCAGTCTC GGGGAATTGC A // |
Primer: pAxCALNL_F1 Sequence file: RDB07292_A3I5c.seq |
>07292_07292_A3I5_pAxCALNL_F1_F11_17_ABI24.ab1 1 ACATGTATCT TATCATGTCT GCTCGAAGCG GCCGGCCGCC CCGACTCTAG AATTATTACA 61 CGGCGATCTT GCCGCCCTTC TTGGCCTTAA TGAGAATCTC GCGGATCTTG CGGGCGTCCA 121 ACTTGCCGGT CAGTCCTTTA GGCACCTCGT CCACGAACAC AACACCACCG CGCAGCTTCT 181 TGGCGGTTGT AACCTGGCTG GCCACATAGT CCACGATCTC CTTCTCGGTC ATGGTTTTAC 241 CGTGTTCCAG CACGACGACT GCGGCGGGCA GCTCGCCGGC ATCGTCGTCG GGCAGGCCGG 301 CGACCCCGGC GTCGAAGATG TTGGGGTGTT GCAGCAGGAT GCTCTCCAGT TCGGCTGGGG 361 CTACCTGGTA GCCCTTGTAT TTGATCAGGC TCTTCAGCCG GTCCACGATG AAGAAGTGCT 421 CGTCCTCGTC CCAGTAGGCG ATGTCGCCGC TGTGCAGCCA GCCGTCCTTG TCGATGAGAG 481 CGTTTGTAGC CTCGGGGTTG TTAACGTAGC CGCTCATGAT CATGGGGCCA CGGACGCACA 541 GCTCGCCGCG CTGGTTCACA CCCAGTGTCT TACCGGTGTC CAAGTCCACC ACCTTAGCCT 601 CGAAGAAGGG CACCACCTTG CCTACTGCGC CAGGCTTGTC GTCCCCTTCG GGGGTGATCA 661 GAATGGCGCT GGTTGTTTCT GTCAGGCCGT AGCCCTGGCG GATGCCTGGT AGGTGGAAGC 721 GTTTGGCCAC GGCCTCACCT ACCTCCTTGC TGAGCGGCGC CCCGCCGCTG GCGATCTCGT 781 GCAAGTTGCT TAGGTCGTAC TTGTCGATGA GAGTGCTCTT AGCGAAGAAG CTAAATAGTG 841 TGGGCACCAG CAGGGCAGAT TGAATCTTAT AGTCTTGCAA GCTGCGCAAG AATAGCTCCT 901 CCTCGAAGCG GTACATGAGC ACGACCCGAA AGCCGCAGAT CAAGTAGCCC AGCGTGGTGA 961 ACATGCCGAA GCCGTGGTGA AATGGCACCA CGCTGAGGAT AGCGGTGTCG GGGATGATCT 1021 GGGTTGCCCG AAGATGGGGT CGCGGGCATG ACTGAATCGG ACACAGCGGT GCCGTGCGTA 1081 GGGCCTACGC CCTTTGGGCA TTCCGGTTAC TGCAACTTAC TGGTCAATGG AATCAAGGCG 1141 ATGGATTTTG TTCCCGGGTT CGCGA // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
user_report | Suzuki, T., Arsenite-induced histone H3 modification and its effects on EGR1 and FOS expression in HeLa cells. J. Appl. Toxicol. 38 (5): 734-743 (2018) PMID 29350772. [link to RRC of NBRP] |
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