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pGL4-phFOS

Promoter collection, Human FOS promoter

Catalog number RDB07292
Resource name pGL4-phFOS
Alternative name U2638 (2016)-3
Clone info. PCR amplified human FOS promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 75277609 to 75278869(NC_000014.9); relatively -1169 to +92, where +1 corresponds to 1 nt of NM_005252.4.
Comment PCR cloning, forward primer: tcccagcagtcgaggtattc; reverse primer: ggctcagtcttggcttctca. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human v-fos FBJ murine osteosarcoma viral oncogene homolog genomic DNA
Depositor|Developer DNA Bank, | Pan, Jianzhi |
Other clones in our bank

External Database
Human v-fos FBJ murine osteosarcoma viral oncogene homolog

          Reference sequence
            

          Distribution information

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          Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          RDB07292 pGL4-phFOS DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (pdf) RDB07292.pdf

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Promoter Collection (Firefly Luciferase) (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB07292_A3I5p1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGL4-4174F
          Sequence file: RDB07292_A3I5a.seq check
          >07292_07292_A3I5_pGL4-4174F_A04_01.ab1
              1 AACTAGGGGC AGACATTTCT CTGGCCTAAC TGGCCGGTAC CTGAGCTCGC TAGCCTCGAG
             61 GATTCCCAGC AGTCGAGGTA TTCCGCCCAG GCGCAGCTGG ACACTGTCCT TCCAGCCCCC
            121 GTCCTCCACC CTCCAAGTCC GCGCTGGAAA ATCACCCGCT GCGGGCTCCC GTAAGCACAG
            181 CTTCCTGGCG GGACCGAACC AGCCCTCAGC GCAGATTTGA GTTCCCCGCA GGAAGCACAC
            241 CCCGCCTTGT CATCCCGAAC TGACCACCCT GCCCACATAA CCACACCTCG CACTCCCTAC
            301 CCCTGGGGCC CAGCTCAGAA CCGGGCAGAC ACCCCCTTCA AATGTCTTCG CACGTAGGTT
            361 TTGCACAGTG TTTATCTGCT GGTGTCTCAG GGATTTGACA GTTTCCTTAA TATTCCCACA
            421 CATGGCCGAG AAAAATAAAT AAATAAATGC GCTGTCTTCT TTAAAAAAAT AAATAAATAA
            481 AGTACCCAGT ATCGTAAAGT AGGTTATCGT ATTCTCTTAT TTTGGATCCT CCACTTTCTG
            541 CTTCCAAACG CAGGAACAGT GCTAGTATTG CTCGAGCCCG AGGGCTGGAG GTTAGGGGAT
            601 GAAGGTCTGC TTCCACGCTT TGCACTGAAT TAGGGCTAGA ATTGGGGATG GGGGTAGGGG
            661 CGCATTCCTT CGGGAGCCGA GGCTTAAGTC CTCGGGGTCC TGTACTCGAT GCCGTTTCTC
            721 CTATCTCTGA GCCTCAGAAC TGTCTTCAGT TTCCGTACAA GGGTAAAAAG GCGCTCTCTG
            781 CCCCATCCCC CCCGACCTCG GGAACAAGGG TCCGCATTGA ACCAGGTGCG AATGTTCTCT
            841 CTCATTCTGC GCCGTTCCCG CCTCCCCTCC CCCAGCCGCG GCCCCCGCCT CCCCCCGCAC
            901 TGCACCCTCG GTGTTGGCTG CAGCCCGCGA GCAGTTCCCG TCAATCCCTC CCCCCTTACA
            961 CAGGATGTCC ATATTAGGAC ATCTGCGTCA GCAGGTTTCC ACGGGCCTTT CCCTGTAGCC
           1021 CTGGGGGGGA GCCATCCCCG AAACCCCTCA TCTTGGGGGG GCCCACGAGA CCTCTGAGAC
           1081 AGGACTGCGA AATGCTCACG GAGGATTAGG ACACGCGCTA AGCGGGGGCA TTAAGCCTGC
          1141 GAGCGCCTGG GGGACCGCCA GTCCGGGCGG ATCGGTCA
          //
          Primer: pGL4-136R
          Sequence file: RDB07292_A3I5b.seq check
          >07292_07292_A3I5_pGL4-136R_B04_04.ab1
              1 GCATTTTTGT TTTTGGCATC TTCCATGGTG GCTTTACCAA CAGTACCGGA TTGCCAAGCT
             61 TGGCCGCCGA GGCCAGATCT TGATGGCTCA GTCTTGGCTT CTCAGATGCT CGCTGCAGAT
            121 GCGGTTGGAG TACGAGGCGC CGCAGCCACT GCTTTTATAA CAAGCGTTTT ATGAATGAGT
            181 GTAAACGTCA CGGGCTCAAC CACGGTGGCG CCAGAGGGGT GGCGCGCGGG CCTGGGCGCT
            241 TCTGCGGCCG CCCGGCTGCG TCCCCAGCGC TCGCAGCTCC CTGCCCCCGC CTTGGCGCGT
            301 GTCCTAATCT CGTGAGCATT TCGCAGTTCC TGTCTCAGAG GTCTCGTGGG CCCCCCAAGA
            361 TGAGGGGTTT CGGGGATGGC TCCCCCCAGG GCTACAGGGA AAGGCCGTGG AAACCTGCTG
            421 ACGCAGATGT CCTAATATGG ACATCCTGTG TAAGGGGGGA GGGATTGACG GGAACTGCTC
            481 GCGGGCTGCA GCCAACACCG AGGGTGCAGT GCGGGGGGAG GCGGGGGCCG CGGCTGGGGG
            541 AGGGGAGGCG GGAACGGCGC AGAATGAGAG AGAACATTCG CACCTGGTTC AATGCGGACC
            601 CTTGTTCCCG AGGTCGGGGG GGATGGGGCA GAGAGCGCCT TTTTACCCTT GTACGGAAAC
            661 TGAAGACAGT TCTGAGGCTC AGAGATAGGA GAAACGGCAT CGAGTACAGG ACCCCGAGGA
            721 CTTAAGCCTC GGCTCCCGAA GGAATGCGCC CCTACCCCCA TCCCCAATTC TAGCCCTAAT
            781 TCAGTGCAAA GCGTGGAAGC AGACCTTCAT CCCCTAACCT CCAGCCCTCG GGCTCGAGCA
            841 ATACTAGCAC TGTTCCTGCG TTTGGAAGCA GAAAGTGGAG GATCCAAAAT AAGAGAATAC
            901 GATAACCTAC TTTACGATAC TGGGTACTTT ATTTATTTAT TTTTTTTAAA GAAGACAGCG
            961 CATTTATTTA TTTATTTTTT CTCGGGCCAT GTGTGGGAAA TATTAAAGGA AACTGTCAAA
           1021 TCCTGAGACA CCAGCAGATA AACACTGTGC AAAACCTACG TGCGAAGACA TTTGAAGGGG
           1081 GTGTCTGCCG GATCTGAGCT GGGACCCCAA GGGCTAAGCA GTGCGAAGTG GTGGTTATGT
          1141 GGGCAGGATG GTCCAGTCTC GGGGAATTGC A
          //
          Primer: pAxCALNL_F1
          Sequence file: RDB07292_A3I5c.seq check
          >07292_07292_A3I5_pAxCALNL_F1_F11_17_ABI24.ab1
              1 ACATGTATCT TATCATGTCT GCTCGAAGCG GCCGGCCGCC CCGACTCTAG AATTATTACA
             61 CGGCGATCTT GCCGCCCTTC TTGGCCTTAA TGAGAATCTC GCGGATCTTG CGGGCGTCCA
            121 ACTTGCCGGT CAGTCCTTTA GGCACCTCGT CCACGAACAC AACACCACCG CGCAGCTTCT
            181 TGGCGGTTGT AACCTGGCTG GCCACATAGT CCACGATCTC CTTCTCGGTC ATGGTTTTAC
            241 CGTGTTCCAG CACGACGACT GCGGCGGGCA GCTCGCCGGC ATCGTCGTCG GGCAGGCCGG
            301 CGACCCCGGC GTCGAAGATG TTGGGGTGTT GCAGCAGGAT GCTCTCCAGT TCGGCTGGGG
            361 CTACCTGGTA GCCCTTGTAT TTGATCAGGC TCTTCAGCCG GTCCACGATG AAGAAGTGCT
            421 CGTCCTCGTC CCAGTAGGCG ATGTCGCCGC TGTGCAGCCA GCCGTCCTTG TCGATGAGAG
            481 CGTTTGTAGC CTCGGGGTTG TTAACGTAGC CGCTCATGAT CATGGGGCCA CGGACGCACA
            541 GCTCGCCGCG CTGGTTCACA CCCAGTGTCT TACCGGTGTC CAAGTCCACC ACCTTAGCCT
            601 CGAAGAAGGG CACCACCTTG CCTACTGCGC CAGGCTTGTC GTCCCCTTCG GGGGTGATCA
            661 GAATGGCGCT GGTTGTTTCT GTCAGGCCGT AGCCCTGGCG GATGCCTGGT AGGTGGAAGC
            721 GTTTGGCCAC GGCCTCACCT ACCTCCTTGC TGAGCGGCGC CCCGCCGCTG GCGATCTCGT
            781 GCAAGTTGCT TAGGTCGTAC TTGTCGATGA GAGTGCTCTT AGCGAAGAAG CTAAATAGTG
            841 TGGGCACCAG CAGGGCAGAT TGAATCTTAT AGTCTTGCAA GCTGCGCAAG AATAGCTCCT
            901 CCTCGAAGCG GTACATGAGC ACGACCCGAA AGCCGCAGAT CAAGTAGCCC AGCGTGGTGA
            961 ACATGCCGAA GCCGTGGTGA AATGGCACCA CGCTGAGGAT AGCGGTGTCG GGGATGATCT
           1021 GGGTTGCCCG AAGATGGGGT CGCGGGCATG ACTGAATCGG ACACAGCGGT GCCGTGCGTA
           1081 GGGCCTACGC CCTTTGGGCA TTCCGGTTAC TGCAACTTAC TGGTCAATGG AATCAAGGCG
          1141 ATGGATTTTG TTCCCGGGTT CGCGA
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          user_report Suzuki, T., Arsenite-induced histone H3 modification and its effects on EGR1 and FOS expression in HeLa cells. J. Appl. Toxicol. 38 (5): 734-743 (2018) PMID 29350772.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl