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pGL4-phFOS (#RDB07292)

Promoter collection, Human FOS promoter

Alternative name U2638 (2016)-3
Clone info. PCR amplified human FOS promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 75277609 to 75278869(NC_000014.9); relatively -1169 to +92, where +1 corresponds to 1 nt of NM_005252.4.
Comment PCR cloning, forward primer: tcccagcagtcgaggtattc; reverse primer: ggctcagtcttggcttctca. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human v-fos FBJ murine osteosarcoma viral oncogene homolog genomic DNA
Depositor|Developer DNA Bank, | Pan, Jianzhi |
 
Remarks, protocol and/or map (pdf) RDB07292.pdf

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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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RDB07292 pGL4-phFOS DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phFOS was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07292).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB07292_A3I5p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F
Sequence file: RDB07292_A3I5a.seq check
>07292_07292_A3I5_pGL4-4174F_A04_01.ab1
    1 AACTAGGGGC AGACATTTCT CTGGCCTAAC TGGCCGGTAC CTGAGCTCGC TAGCCTCGAG
   61 GATTCCCAGC AGTCGAGGTA TTCCGCCCAG GCGCAGCTGG ACACTGTCCT TCCAGCCCCC
  121 GTCCTCCACC CTCCAAGTCC GCGCTGGAAA ATCACCCGCT GCGGGCTCCC GTAAGCACAG
  181 CTTCCTGGCG GGACCGAACC AGCCCTCAGC GCAGATTTGA GTTCCCCGCA GGAAGCACAC
  241 CCCGCCTTGT CATCCCGAAC TGACCACCCT GCCCACATAA CCACACCTCG CACTCCCTAC
  301 CCCTGGGGCC CAGCTCAGAA CCGGGCAGAC ACCCCCTTCA AATGTCTTCG CACGTAGGTT
  361 TTGCACAGTG TTTATCTGCT GGTGTCTCAG GGATTTGACA GTTTCCTTAA TATTCCCACA
  421 CATGGCCGAG AAAAATAAAT AAATAAATGC GCTGTCTTCT TTAAAAAAAT AAATAAATAA
  481 AGTACCCAGT ATCGTAAAGT AGGTTATCGT ATTCTCTTAT TTTGGATCCT CCACTTTCTG
  541 CTTCCAAACG CAGGAACAGT GCTAGTATTG CTCGAGCCCG AGGGCTGGAG GTTAGGGGAT
  601 GAAGGTCTGC TTCCACGCTT TGCACTGAAT TAGGGCTAGA ATTGGGGATG GGGGTAGGGG
  661 CGCATTCCTT CGGGAGCCGA GGCTTAAGTC CTCGGGGTCC TGTACTCGAT GCCGTTTCTC
  721 CTATCTCTGA GCCTCAGAAC TGTCTTCAGT TTCCGTACAA GGGTAAAAAG GCGCTCTCTG
  781 CCCCATCCCC CCCGACCTCG GGAACAAGGG TCCGCATTGA ACCAGGTGCG AATGTTCTCT
  841 CTCATTCTGC GCCGTTCCCG CCTCCCCTCC CCCAGCCGCG GCCCCCGCCT CCCCCCGCAC
  901 TGCACCCTCG GTGTTGGCTG CAGCCCGCGA GCAGTTCCCG TCAATCCCTC CCCCCTTACA
  961 CAGGATGTCC ATATTAGGAC ATCTGCGTCA GCAGGTTTCC ACGGGCCTTT CCCTGTAGCC
 1021 CTGGGGGGGA GCCATCCCCG AAACCCCTCA TCTTGGGGGG GCCCACGAGA CCTCTGAGAC
 1081 AGGACTGCGA AATGCTCACG GAGGATTAGG ACACGCGCTA AGCGGGGGCA TTAAGCCTGC
1141 GAGCGCCTGG GGGACCGCCA GTCCGGGCGG ATCGGTCA
//
Primer: pGL4-136R
Sequence file: RDB07292_A3I5b.seq check
>07292_07292_A3I5_pGL4-136R_B04_04.ab1
    1 GCATTTTTGT TTTTGGCATC TTCCATGGTG GCTTTACCAA CAGTACCGGA TTGCCAAGCT
   61 TGGCCGCCGA GGCCAGATCT TGATGGCTCA GTCTTGGCTT CTCAGATGCT CGCTGCAGAT
  121 GCGGTTGGAG TACGAGGCGC CGCAGCCACT GCTTTTATAA CAAGCGTTTT ATGAATGAGT
  181 GTAAACGTCA CGGGCTCAAC CACGGTGGCG CCAGAGGGGT GGCGCGCGGG CCTGGGCGCT
  241 TCTGCGGCCG CCCGGCTGCG TCCCCAGCGC TCGCAGCTCC CTGCCCCCGC CTTGGCGCGT
  301 GTCCTAATCT CGTGAGCATT TCGCAGTTCC TGTCTCAGAG GTCTCGTGGG CCCCCCAAGA
  361 TGAGGGGTTT CGGGGATGGC TCCCCCCAGG GCTACAGGGA AAGGCCGTGG AAACCTGCTG
  421 ACGCAGATGT CCTAATATGG ACATCCTGTG TAAGGGGGGA GGGATTGACG GGAACTGCTC
  481 GCGGGCTGCA GCCAACACCG AGGGTGCAGT GCGGGGGGAG GCGGGGGCCG CGGCTGGGGG
  541 AGGGGAGGCG GGAACGGCGC AGAATGAGAG AGAACATTCG CACCTGGTTC AATGCGGACC
  601 CTTGTTCCCG AGGTCGGGGG GGATGGGGCA GAGAGCGCCT TTTTACCCTT GTACGGAAAC
  661 TGAAGACAGT TCTGAGGCTC AGAGATAGGA GAAACGGCAT CGAGTACAGG ACCCCGAGGA
  721 CTTAAGCCTC GGCTCCCGAA GGAATGCGCC CCTACCCCCA TCCCCAATTC TAGCCCTAAT
  781 TCAGTGCAAA GCGTGGAAGC AGACCTTCAT CCCCTAACCT CCAGCCCTCG GGCTCGAGCA
  841 ATACTAGCAC TGTTCCTGCG TTTGGAAGCA GAAAGTGGAG GATCCAAAAT AAGAGAATAC
  901 GATAACCTAC TTTACGATAC TGGGTACTTT ATTTATTTAT TTTTTTTAAA GAAGACAGCG
  961 CATTTATTTA TTTATTTTTT CTCGGGCCAT GTGTGGGAAA TATTAAAGGA AACTGTCAAA
 1021 TCCTGAGACA CCAGCAGATA AACACTGTGC AAAACCTACG TGCGAAGACA TTTGAAGGGG
 1081 GTGTCTGCCG GATCTGAGCT GGGACCCCAA GGGCTAAGCA GTGCGAAGTG GTGGTTATGT
1141 GGGCAGGATG GTCCAGTCTC GGGGAATTGC A
//
Primer: pAxCALNL_F1
Sequence file: RDB07292_A3I5c.seq check
>07292_07292_A3I5_pAxCALNL_F1_F11_17_ABI24.ab1
    1 ACATGTATCT TATCATGTCT GCTCGAAGCG GCCGGCCGCC CCGACTCTAG AATTATTACA
   61 CGGCGATCTT GCCGCCCTTC TTGGCCTTAA TGAGAATCTC GCGGATCTTG CGGGCGTCCA
  121 ACTTGCCGGT CAGTCCTTTA GGCACCTCGT CCACGAACAC AACACCACCG CGCAGCTTCT
  181 TGGCGGTTGT AACCTGGCTG GCCACATAGT CCACGATCTC CTTCTCGGTC ATGGTTTTAC
  241 CGTGTTCCAG CACGACGACT GCGGCGGGCA GCTCGCCGGC ATCGTCGTCG GGCAGGCCGG
  301 CGACCCCGGC GTCGAAGATG TTGGGGTGTT GCAGCAGGAT GCTCTCCAGT TCGGCTGGGG
  361 CTACCTGGTA GCCCTTGTAT TTGATCAGGC TCTTCAGCCG GTCCACGATG AAGAAGTGCT
  421 CGTCCTCGTC CCAGTAGGCG ATGTCGCCGC TGTGCAGCCA GCCGTCCTTG TCGATGAGAG
  481 CGTTTGTAGC CTCGGGGTTG TTAACGTAGC CGCTCATGAT CATGGGGCCA CGGACGCACA
  541 GCTCGCCGCG CTGGTTCACA CCCAGTGTCT TACCGGTGTC CAAGTCCACC ACCTTAGCCT
  601 CGAAGAAGGG CACCACCTTG CCTACTGCGC CAGGCTTGTC GTCCCCTTCG GGGGTGATCA
  661 GAATGGCGCT GGTTGTTTCT GTCAGGCCGT AGCCCTGGCG GATGCCTGGT AGGTGGAAGC
  721 GTTTGGCCAC GGCCTCACCT ACCTCCTTGC TGAGCGGCGC CCCGCCGCTG GCGATCTCGT
  781 GCAAGTTGCT TAGGTCGTAC TTGTCGATGA GAGTGCTCTT AGCGAAGAAG CTAAATAGTG
  841 TGGGCACCAG CAGGGCAGAT TGAATCTTAT AGTCTTGCAA GCTGCGCAAG AATAGCTCCT
  901 CCTCGAAGCG GTACATGAGC ACGACCCGAA AGCCGCAGAT CAAGTAGCCC AGCGTGGTGA
  961 ACATGCCGAA GCCGTGGTGA AATGGCACCA CGCTGAGGAT AGCGGTGTCG GGGATGATCT
 1021 GGGTTGCCCG AAGATGGGGT CGCGGGCATG ACTGAATCGG ACACAGCGGT GCCGTGCGTA
 1081 GGGCCTACGC CCTTTGGGCA TTCCGGTTAC TGCAACTTAC TGGTCAATGG AATCAAGGCG
1141 ATGGATTTTG TTCCCGGGTT CGCGA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

user_report Suzuki, T., Arsenite-induced histone H3 modification and its effects on EGR1 and FOS expression in HeLa cells. J. Appl. Toxicol. 38 (5): 734-743 (2018) PMID 29350772. [link to RRC of NBRP]