Resource data sheet
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pSRR2 tk-luc

Oct-Sox enhancer carrying reporter derived from Sox2.

Catalog number RDB07095
Resource name pSRR2 tk-luc
Alternative name pSox2-luc, stock no 1419
Clone info. Reporter construct with luciferase expression under the Sox2 3' enhancer.
Use ptk-luc (RDB07031; stock no. 649) as a control.
Vector backbone ptk-luc (Plasmid)
Selectable markers Amp^r
Gene/insert name Mouse Sox2 cDNA
Depositor Niwa, Hitoshi |
 
Other clones in our bank mouse Sox2 (NCBI Gene 20674) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Tomioka, M., Nuc. Acids Res., 30 (14), 3202-3213, 2002)
Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB07095 pSRR2 tk-luc DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB07095z.seq from Depositor
Electronic file Map RDB07095a.gif from Depositor

Featured content

References

original Tomioka, M., Identification of Sox-2 regulatory region which is under the control of Oct-3/4-Sox-2 complex. Nuc. Acids Res., 30 (14), 3202-3213, 2002. PMID 12136102.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB07095_A4E7.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F RDB07095_A4E7a.seq
>07095_07095_A4E7_1_pGL4-4174F_A02_02.ab1
    1 AGTAGGGTCA GACATTTCTC TATCGATAGG TACCGAGCTC TTACGCGTGC TAGCCCGGGC
   61 TCGAGATCCG GCCCCGCCCA GCGTCTTGTC ATTGGCGAAT TCGAACACGC AGATGCAGTC
  121 GGGGCGGCGC GGTCCGAGGT CCACTTCGCA TATTAAGGTG ACGCGTGTGG CCTCGAACAC
  181 CGAGCGACCC TGCAGCGACC CGCTTAACAG CGTCAACAGC GTGCCGCAGA TCTCGAAGCT
  241 TGGCATTCCG GTACTGTTGG TAAAGCCACC ATGGAAGACG CCAAAAACAT AAAGAAAGGC
  301 CCGGCGCCAT TCTATCCGCT GGAAGATGGA ACCGCTGGAG AGCAACTGCA TAAGGCTATG
  361 AAGAGATACG CCCTGGTTCC TGGAACAATT GCTTTTACAG ATGCACATAT CGAGGTGGAC
  421 ATCACTTACG CTGAGTACTT CGAAATGTCC GTTCGGTTGG CAGAAGCTAT GAAACGATAT
  481 GGGCTGAATA CAAATCACAG AATCGTCGTA TGCAGTGAAA ACTCTCTTCA ATTCTTTATG
  541 CCGGTGTTGG GCGCGTTATT TATCGGAGTT GCAGTTGCGC CCGCGAACGA CATTTATAAT
  601 GAACGTGAAT TGCTCAACAG TATGGGCATT TCGCAGCCTA CCGTGGTGTT CGTTTCCAAA
  661 AAGGGGTTGC AAAAAATTTT GAACGTGCAA AAAAAGCTCC CAATCATCCA AAAAATTATT
  721 ATCATGGATT CTAAAACGGA TTACCAGGGA TTTCAGTCGA TGTACACGTT CGTCACATCT
  781 CATCTACCTC CCGGTTTTAA TGAATACGAT TTTGTGCCAG AGTCCTTCGA TAGGGACAAG
  841 ACAATTGCAC TGATCATGAA CTCCTCTGGA TCTACTGGTC TGCCTAAAGG TGTCGCTCTG
  901 CCTCATAGAA CTGCCTGCGT GAGATTCTCG CATGCCAGAG ATCCTATTTT TGGCAATCAA
  961 ATCATTCCGG ATACTGCGAT TTTAAGTGTT GTTCCATTCC ATCACGGTTT TGGAATGTTT
 1021 ACTACACTCG GATATTTGAT ATGTGGATTT CGAGTCGTCT TTAATGTATA GATTTGGAAG
 1081 AGAGCTGTTT TCTGAGGAGC CTTTCATGAA TACAGGAATC AAGTGCGCTG CTGGATGCCA
1141 CCCCTAATTT CCTCCTTTTC TTTCCGGC
//
Primer: SV40pA3'Element Reverse RDB07095_A4E7b.seq
>07095_07095_A4E7_1_SV40pA3ElementReverse_D02_11.ab1
    1 GCGGTTCGGC GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC
   61 TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG
  121 ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC
  181 GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC
  241 ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG
  301 GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC TGTGCTCATT ACCACGTGAA
  361 TAATCCTATA TGCATCACAA TGGCTGCCCG AGCCCGGGAA ATTCTTTTAG AGTCTCTGCA
  421 TTAATTAGGG ATCCTTATCG ATTTTACCAC ATTTGTAGAG GTTTTACTTG CTTTAAAAAA
  481 CCTCCCACAC CTCCCCCTGA ACCTGAAACA TAAAATGAAT GCAATTGTTG TTGTTAACTT
  541 GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA
  601 AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA
  661 TGTCTGCTCG AAGCGGCCGG CCGCCCCGAC TCTAGAATTA CACGGCGATC TTTCCGCCCT
  721 TCTTGGCCTT TATGAGGATC TCTCTGATTT TTCTTGCGTC GAGTTTTCCG GTAAGACCTT
  781 TCGGTACTTC GTCCACAAAC ACAACTCCTC CGCGCAACTT TTTCGCGGTT GTTACTTGAC
  841 TGGCGACGTA ATCCACGATC TCTTTTTCCG TCATCGTCTT TCCGTGCTCC AAACAACAAC
  901 GGCGGCGGGA AGTTCACCGG CGTCATCGTC GGGAAGACCT GCGACACCTG CGTCGAAGAT
  961 GTTGGGGGTG TTGGAGCAAG ATGGATTCCA ATTTCAGCGG GAGCCACCTG ATAGCCCTTT
 1021 GTACTTTAAT CAGAGGACTT CAGGCGGGTC CACGATGAGA AGTGTTTCGT CTTTCCGTCC
 1081 CAGTAAGCTA TGTCCTCCAG CATGTAGTCA TTCATCTTGT CAATCAAGCC GTTGGTCGCC
1141 TTCCGGGGAA TGTG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Tomioka, M., Identification of Sox-2 regulatory region which is under the control of Oct-3/4-Sox-2 complex. Nuc. Acids Res., 30 (14), 3202-3213, 2002. PMID 12136102.
reference Masui, S., Pluripotency governed by Sox2 via regulation of Oct3/4 expression in mouse embryonic stem cells. Nat. Cell Biol., 9, 625-635 (2007). PMID 17515932 .

2018.12.10

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