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pxCAhFLPe (#RDB07083)

Expression vector of site-specific recombinase, FLP

Clone info. Expression vector of site-specific recombinase, FLP. The hFLPe recombinase is expressed under the control of a CAG promoter. To increase the FLPe level in mammalian cells, a humanized FLPe (hFLPe) containing a codon suitable for use in mammals was generated.
Vector backbone pBR327 (Plasmid)
Selectable markers Amp^r
Gene/insert name S. cerevisiae FLP recombinase cDNA
Reference of insert sequence AB434280 (DDBJ accession number)
Depositor|Developer Saito, Izumu | Kondo, Saki |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (J. Mol. Biol., 390, 221-230 (2009)) and an acknowledgment to the DEPOSITOR are requested.
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (J. Mol. Biol., 390, 221-230 (2009)) を引用し、謝辞の表明を必要とする。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB07083 pxCAhFLPe DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pxCAhFLPe was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07083).

Reference section:

Kondo, S., Takata, Y., Nakano, M., Saito, I., Kanegae, Y., Activities of various FLP recombinases expressed by adenovirus vectors in mammalian cells. J. Mol. Biol., 390, 221-230 (2009). PMID 19414019. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13038_A4Lg.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1
Sequence file: RDB13038_A4Lga.seq check
>07083_13038_A4Lg_pAxCA_F1_G12_21.ab1
    1 GGTGCCCGGA AAACCCACGC CGCTCATGTT ATGCCTTCTT CTTTTTCCTA CAGCTCCTGG
   61 GCAACGTGCT GGTTATTGTG CTGTCTCATC ATTTTGGCAA AGAATTGATT TATCGATTTG
  121 CAGACCGTGC ATCATGAGCC AGTTTGATAT CCTGTGCAAG ACACCACCTA AGGTGCTGGT
  181 GCGCCAGTTC GTGGAGAGGT TTGAGAGGCC CTCTGGAGAG AAGATTGCCT CCTGTGCAGC
  241 TGAGCTGACC TACCTGTGCT GGATGATCAC CCACAACGGC ACAGCCATCA AGAGGGCCAC
  301 CTTTATGAGC TACAACACCA TCATTAGCAA CTCCCTGAGC TTCGACATTG TGAACAAGTC
  361 CCTCCAGTTT AAATACAAGA CCCAGAAGGC CACAATCCTG GAGGCCTCCC TGAAGAAATT
  421 GATTCCTGCT TGGGAGTTCA CCATCATCCC CTACAATGGC CAGAAGCACC AGTCTGATAT
  481 CACTGATATT GTGAGCAGTC TGCAACTCCA GTTCGAGTCC TCTGAGGAAG CTGACAAGGG
  541 CAACAGCCAC AGCAAGAAGA TGCTGAAGGC CCTGCTCAGT GAGGGAGAAA GCATCTGGGA
  601 GATCACTGAG AAGATCCTGA ACTCCTTTGA GTACACTTCC AGATTCACCA AGACCAAGAC
  661 CTTGTACCAG TTCCTGTTCC TGGCCACCTT CATCAACTGT GGCAGGTTCA GCGACATCAA
  721 GAATGTGGAT CCCAAATCCT TTAAACTGGT CCAGAACAAG TACCTGGGAG TGATCATCCA
  781 GTGCCTGGTG ACAGAGACCA AGACCTCTGT GAGCAGGCAC ATCTACTTCT TCTCTGCCAG
  841 GGGCAGGATT GATCCACTGG TGTACCTGGA TGAGTTCCTG AGGAACTCTG AGCCAGTGCT
  901 GAAGCGGGTG AACAGGACCG GCAACTCTTC CAGCAACAAG CAGGAGTACC AGCTGCTCAA
  961 AGGACAACCT GGTGAGGTCC TACAACAAAG CTTTGAAGAA AAATGCCCCC CTACCCAATC
 1021 TTTGCCATCA AGAATGGGCC CTAAGTCCCA CATTTGGCAG ACACCTGATG ACCTCGTTCT
 1081 GTCCATGAAA GGCCCTGAAC GAGCCTGAAC AATGGATGTG GGCCAACTGG AGCCGGAATA
1141 GCCGGGGGCC CTCTCTGGCC CGT
//
Primer: pAxCA_R1
Sequence file: RDB13038_A4Lgb.seq check
>07083_13038_A4Lg_pAxCA_R1_G03_21.ab1
    1 CAAGGGACCC AGCCGCCCTT CGCCACACCA GCCCACCACC TTCTGAATAG GCAGCCCTGC
   61 ACCTTGAGGA GTGAATTGAT TATCGATTCT AGACTAGTTT ATAATTTGTA CCTCTAGAGT
  121 CAGATGCGTC TGTTGATGTA GGAAGACAGG TAGTCCAGCA CCTCCTGGGA AATGATCCCA
  181 TTCCAGGCAG GGTAGCGAAT GCTGCCCTCG GCACTACCCT TCAGCTGCTC AATGTGCTGC
  241 CACTCCTCAA TTGGGTTGGT CTCATCCTTC AATGCAATCA TCTCCTTGGA GATGGGATCA
  301 TATGCATAGT ACCGAGACAC CAGTGCGAAG TAGTGATCAG GGATTGCTGT GATCTGGTGA
  361 GTATAGGTTG TTCTGGCCAC GGCAGAGGCC CGCTTATCGC TCCAGTTGCC CACAACATTG
  421 GTCAGCTCTG TCAGGCCCTT CATGGACAGG AAGGAGGTCA TCAGGTGTCT GCCAATGTGG
  481 GACTTAGGGC CATTCTTGAT GGCAAAGATT GGGTAGGGGG CATTTTTCTT CAAAGCTTTG
  541 TTGTAGGACC TCACCAGGTT GTCCTTGAGC AGCTGGTACT CCTGCTTGTT GCTGGAAGAG
  601 TTGCCGGTCC TGTTCACCCG CTTCAGCACT GGCTCAGAGT TCCTCAGGAA CTCATCCAGG
  661 TACACCCAGT GGATCAATCC TGCCCCTGGC AGAGAAGAAG TAGATGTGCC TGCTCACAGA
  721 GGTCTTGGTC TCTGTCACCA GGCACTGGAT GATCACTCCC AGGTACTTGT TCTGGACCAG
  781 TTTAAAAGGA TTTGGGATCC ACATTCTTGA TGTCGCTGAA CCCTGCCACA GTTGATGAAA
  841 GGTGGCCAGA ACAGGAACTG GTACAAGGTC TTGGGTCCTT GGTGAATCTG GAATGTACTC
901 AAAGAGTTCA AGATCTTCTT CAGTGATCTC CCAGATGCTT TTCTCCCTCA CTGAACC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kondo, S., Activities of various FLP recombinases expressed by adenovirus vectors in mammalian cells. J. Mol. Biol., 390, 221-230 (2009). PMID 19414019. [link to RRC of NBRP]