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pxCAhFLPe

Expression vector of site-specific recombinase, FLP

Catalog number RDB07083
Resource name pxCAhFLPe
Clone info. Expression vector of site-specific recombinase, FLP. The hFLPe recombinase is expressed under the control of a CAG promoter. To increase the FLPe level in mammalian cells, a humanized FLPe (hFLPe) containing a codon suitable for use in mammals was generated.
Vector backbone pBR327 (Plasmid)
Selectable markers Amp^r
Gene/insert name S. cerevisiae FLP recombinase cDNA
Reference of nucleotide sequence AB434280 (DDBJ accession number)
Depositor Saito, Izumu | Kondo, Saki |
 
Other clones in our bank S. cerevisiae FLP |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (J. Mol. Biol., 390, 221-230 (2009)) and an acknowledgment to the DEPOSITOR are requested.
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RDB07083 pxCAhFLPe DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content Cre-loxP and FLP-FRT System (English text)
Featured content Control Vectors (English text)

References

original Kondo, S., Activities of various FLP recombinases expressed by adenovirus vectors in mammalian cells. J. Mol. Biol., 390, 221-230 (2009). PMID 19414019.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13038_A4Lg.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 RDB13038_A4Lga.seq
>07083_13038_A4Lg_pAxCA_F1_G12_21.ab1
    1 GGTGCCCGGA AAACCCACGC CGCTCATGTT ATGCCTTCTT CTTTTTCCTA CAGCTCCTGG
   61 GCAACGTGCT GGTTATTGTG CTGTCTCATC ATTTTGGCAA AGAATTGATT TATCGATTTG
  121 CAGACCGTGC ATCATGAGCC AGTTTGATAT CCTGTGCAAG ACACCACCTA AGGTGCTGGT
  181 GCGCCAGTTC GTGGAGAGGT TTGAGAGGCC CTCTGGAGAG AAGATTGCCT CCTGTGCAGC
  241 TGAGCTGACC TACCTGTGCT GGATGATCAC CCACAACGGC ACAGCCATCA AGAGGGCCAC
  301 CTTTATGAGC TACAACACCA TCATTAGCAA CTCCCTGAGC TTCGACATTG TGAACAAGTC
  361 CCTCCAGTTT AAATACAAGA CCCAGAAGGC CACAATCCTG GAGGCCTCCC TGAAGAAATT
  421 GATTCCTGCT TGGGAGTTCA CCATCATCCC CTACAATGGC CAGAAGCACC AGTCTGATAT
  481 CACTGATATT GTGAGCAGTC TGCAACTCCA GTTCGAGTCC TCTGAGGAAG CTGACAAGGG
  541 CAACAGCCAC AGCAAGAAGA TGCTGAAGGC CCTGCTCAGT GAGGGAGAAA GCATCTGGGA
  601 GATCACTGAG AAGATCCTGA ACTCCTTTGA GTACACTTCC AGATTCACCA AGACCAAGAC
  661 CTTGTACCAG TTCCTGTTCC TGGCCACCTT CATCAACTGT GGCAGGTTCA GCGACATCAA
  721 GAATGTGGAT CCCAAATCCT TTAAACTGGT CCAGAACAAG TACCTGGGAG TGATCATCCA
  781 GTGCCTGGTG ACAGAGACCA AGACCTCTGT GAGCAGGCAC ATCTACTTCT TCTCTGCCAG
  841 GGGCAGGATT GATCCACTGG TGTACCTGGA TGAGTTCCTG AGGAACTCTG AGCCAGTGCT
  901 GAAGCGGGTG AACAGGACCG GCAACTCTTC CAGCAACAAG CAGGAGTACC AGCTGCTCAA
  961 AGGACAACCT GGTGAGGTCC TACAACAAAG CTTTGAAGAA AAATGCCCCC CTACCCAATC
 1021 TTTGCCATCA AGAATGGGCC CTAAGTCCCA CATTTGGCAG ACACCTGATG ACCTCGTTCT
 1081 GTCCATGAAA GGCCCTGAAC GAGCCTGAAC AATGGATGTG GGCCAACTGG AGCCGGAATA
1141 GCCGGGGGCC CTCTCTGGCC CGT
//
Primer: pAxCA_R1 RDB13038_A4Lgb.seq
>07083_13038_A4Lg_pAxCA_R1_G03_21.ab1
    1 CAAGGGACCC AGCCGCCCTT CGCCACACCA GCCCACCACC TTCTGAATAG GCAGCCCTGC
   61 ACCTTGAGGA GTGAATTGAT TATCGATTCT AGACTAGTTT ATAATTTGTA CCTCTAGAGT
  121 CAGATGCGTC TGTTGATGTA GGAAGACAGG TAGTCCAGCA CCTCCTGGGA AATGATCCCA
  181 TTCCAGGCAG GGTAGCGAAT GCTGCCCTCG GCACTACCCT TCAGCTGCTC AATGTGCTGC
  241 CACTCCTCAA TTGGGTTGGT CTCATCCTTC AATGCAATCA TCTCCTTGGA GATGGGATCA
  301 TATGCATAGT ACCGAGACAC CAGTGCGAAG TAGTGATCAG GGATTGCTGT GATCTGGTGA
  361 GTATAGGTTG TTCTGGCCAC GGCAGAGGCC CGCTTATCGC TCCAGTTGCC CACAACATTG
  421 GTCAGCTCTG TCAGGCCCTT CATGGACAGG AAGGAGGTCA TCAGGTGTCT GCCAATGTGG
  481 GACTTAGGGC CATTCTTGAT GGCAAAGATT GGGTAGGGGG CATTTTTCTT CAAAGCTTTG
  541 TTGTAGGACC TCACCAGGTT GTCCTTGAGC AGCTGGTACT CCTGCTTGTT GCTGGAAGAG
  601 TTGCCGGTCC TGTTCACCCG CTTCAGCACT GGCTCAGAGT TCCTCAGGAA CTCATCCAGG
  661 TACACCCAGT GGATCAATCC TGCCCCTGGC AGAGAAGAAG TAGATGTGCC TGCTCACAGA
  721 GGTCTTGGTC TCTGTCACCA GGCACTGGAT GATCACTCCC AGGTACTTGT TCTGGACCAG
  781 TTTAAAAGGA TTTGGGATCC ACATTCTTGA TGTCGCTGAA CCCTGCCACA GTTGATGAAA
  841 GGTGGCCAGA ACAGGAACTG GTACAAGGTC TTGGGTCCTT GGTGAATCTG GAATGTACTC
901 AAAGAGTTCA AGATCTTCTT CAGTGATCTC CCAGATGCTT TTCTCCCTCA CTGAACC
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Kondo, S., Activities of various FLP recombinases expressed by adenovirus vectors in mammalian cells. J. Mol. Biol., 390, 221-230 (2009). PMID 19414019.

2018.12.10

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