Expression vector of site-specific recombinase, FLP
Clone info. | Expression vector of site-specific recombinase, FLP. The hFLPe recombinase is expressed under the control of a CAG promoter. To increase the FLPe level in mammalian cells, a humanized FLPe (hFLPe) containing a codon suitable for use in mammals was generated. |
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Vector backbone | pBR327 (Plasmid) |
Selectable markers | Amp^r |
Gene/insert name | S. cerevisiae FLP recombinase cDNA |
Reference of insert sequence | AB434280 (DDBJ accession number) |
Depositor|Developer | Saito, Izumu | Kondo, Saki | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (J. Mol. Biol., 390, 221-230 (2009)) and an acknowledgment to the DEPOSITOR are requested. Additional terms and conditions: Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested. |
必要書類 | 提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献 (J. Mol. Biol., 390, 221-230 (2009)) を引用し、謝辞の表明を必要とする。 MTAに書く付加的使用条件: CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB07083 | pxCAhFLPe | DNA solution |
Materials & Methods section:
The pxCAhFLPe was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07083). |
Reference section:
Kondo, S., Takata, Y., Nakano, M., Saito, I., Kanegae, Y., Activities of various FLP recombinases expressed by adenovirus vectors in mammalian cells. J. Mol. Biol., 390, 221-230 (2009). PMID 19414019. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB13038_A4Lg.pdf |
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Nucleotide sequence of a portion of this resource (if available).
Primer: pAxCA_F1 Sequence file: RDB13038_A4Lga.seq |
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>07083_13038_A4Lg_pAxCA_F1_G12_21.ab1 1 GGTGCCCGGA AAACCCACGC CGCTCATGTT ATGCCTTCTT CTTTTTCCTA CAGCTCCTGG 61 GCAACGTGCT GGTTATTGTG CTGTCTCATC ATTTTGGCAA AGAATTGATT TATCGATTTG 121 CAGACCGTGC ATCATGAGCC AGTTTGATAT CCTGTGCAAG ACACCACCTA AGGTGCTGGT 181 GCGCCAGTTC GTGGAGAGGT TTGAGAGGCC CTCTGGAGAG AAGATTGCCT CCTGTGCAGC 241 TGAGCTGACC TACCTGTGCT GGATGATCAC CCACAACGGC ACAGCCATCA AGAGGGCCAC 301 CTTTATGAGC TACAACACCA TCATTAGCAA CTCCCTGAGC TTCGACATTG TGAACAAGTC 361 CCTCCAGTTT AAATACAAGA CCCAGAAGGC CACAATCCTG GAGGCCTCCC TGAAGAAATT 421 GATTCCTGCT TGGGAGTTCA CCATCATCCC CTACAATGGC CAGAAGCACC AGTCTGATAT 481 CACTGATATT GTGAGCAGTC TGCAACTCCA GTTCGAGTCC TCTGAGGAAG CTGACAAGGG 541 CAACAGCCAC AGCAAGAAGA TGCTGAAGGC CCTGCTCAGT GAGGGAGAAA GCATCTGGGA 601 GATCACTGAG AAGATCCTGA ACTCCTTTGA GTACACTTCC AGATTCACCA AGACCAAGAC 661 CTTGTACCAG TTCCTGTTCC TGGCCACCTT CATCAACTGT GGCAGGTTCA GCGACATCAA 721 GAATGTGGAT CCCAAATCCT TTAAACTGGT CCAGAACAAG TACCTGGGAG TGATCATCCA 781 GTGCCTGGTG ACAGAGACCA AGACCTCTGT GAGCAGGCAC ATCTACTTCT TCTCTGCCAG 841 GGGCAGGATT GATCCACTGG TGTACCTGGA TGAGTTCCTG AGGAACTCTG AGCCAGTGCT 901 GAAGCGGGTG AACAGGACCG GCAACTCTTC CAGCAACAAG CAGGAGTACC AGCTGCTCAA 961 AGGACAACCT GGTGAGGTCC TACAACAAAG CTTTGAAGAA AAATGCCCCC CTACCCAATC 1021 TTTGCCATCA AGAATGGGCC CTAAGTCCCA CATTTGGCAG ACACCTGATG ACCTCGTTCT 1081 GTCCATGAAA GGCCCTGAAC GAGCCTGAAC AATGGATGTG GGCCAACTGG AGCCGGAATA 1141 GCCGGGGGCC CTCTCTGGCC CGT // |
Primer: pAxCA_R1 Sequence file: RDB13038_A4Lgb.seq |
>07083_13038_A4Lg_pAxCA_R1_G03_21.ab1 1 CAAGGGACCC AGCCGCCCTT CGCCACACCA GCCCACCACC TTCTGAATAG GCAGCCCTGC 61 ACCTTGAGGA GTGAATTGAT TATCGATTCT AGACTAGTTT ATAATTTGTA CCTCTAGAGT 121 CAGATGCGTC TGTTGATGTA GGAAGACAGG TAGTCCAGCA CCTCCTGGGA AATGATCCCA 181 TTCCAGGCAG GGTAGCGAAT GCTGCCCTCG GCACTACCCT TCAGCTGCTC AATGTGCTGC 241 CACTCCTCAA TTGGGTTGGT CTCATCCTTC AATGCAATCA TCTCCTTGGA GATGGGATCA 301 TATGCATAGT ACCGAGACAC CAGTGCGAAG TAGTGATCAG GGATTGCTGT GATCTGGTGA 361 GTATAGGTTG TTCTGGCCAC GGCAGAGGCC CGCTTATCGC TCCAGTTGCC CACAACATTG 421 GTCAGCTCTG TCAGGCCCTT CATGGACAGG AAGGAGGTCA TCAGGTGTCT GCCAATGTGG 481 GACTTAGGGC CATTCTTGAT GGCAAAGATT GGGTAGGGGG CATTTTTCTT CAAAGCTTTG 541 TTGTAGGACC TCACCAGGTT GTCCTTGAGC AGCTGGTACT CCTGCTTGTT GCTGGAAGAG 601 TTGCCGGTCC TGTTCACCCG CTTCAGCACT GGCTCAGAGT TCCTCAGGAA CTCATCCAGG 661 TACACCCAGT GGATCAATCC TGCCCCTGGC AGAGAAGAAG TAGATGTGCC TGCTCACAGA 721 GGTCTTGGTC TCTGTCACCA GGCACTGGAT GATCACTCCC AGGTACTTGT TCTGGACCAG 781 TTTAAAAGGA TTTGGGATCC ACATTCTTGA TGTCGCTGAA CCCTGCCACA GTTGATGAAA 841 GGTGGCCAGA ACAGGAACTG GTACAAGGTC TTGGGTCCTT GGTGAATCTG GAATGTACTC 901 AAAGAGTTCA AGATCTTCTT CAGTGATCTC CCAGATGCTT TTCTCCCTCA CTGAACC // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Kondo, S., Activities of various FLP recombinases expressed by adenovirus vectors in mammalian cells. J. Mol. Biol., 390, 221-230 (2009). PMID 19414019. [link to RRC of NBRP] |
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