Sequencing and PCR primers for primer information.
Expression vector of site-specific recombinase, FLP
| Catalog number | RDB07083 |
|---|---|
| Resource name | pxCAhFLPe |
| Clone info. | Expression vector of site-specific recombinase, FLP. The hFLPe recombinase is expressed under the control of a CAG promoter. To increase the FLPe level in mammalian cells, a humanized FLPe (hFLPe) containing a codon suitable for use in mammals was generated. |
| Vector backbone | pBR327 (Plasmid) |
| Selectable markers | Amp^r |
| Gene/insert name | S. cerevisiae FLP recombinase cDNA |
| Reference of nucleotide sequence | AB434280 (DDBJ accession number) |
| Depositor | Saito, Izumu | Kondo, Saki | |
| Other clones in our bank | S. cerevisiae FLP | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (J. Mol. Biol., 390, 221-230 (2009)) and an acknowledgment to the DEPOSITOR are requested. |
|---|---|
| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB07083 | pxCAhFLPe | DNA solution |
Electronic file
Featured content
| Featured content | Cre-loxP and FLP-FRT System (English text) |
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| Featured content | Control Vectors (English text) |
References
| original | Kondo, S., Activities of various FLP recombinases expressed by adenovirus vectors in mammalian cells. J. Mol. Biol., 390, 221-230 (2009). PMID 19414019. |
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User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB13038_A4Lg.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: pAxCA_F1 | RDB13038_A4Lga.seq |
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>07083_13038_A4Lg_pAxCA_F1_G12_21.ab1 1 GGTGCCCGGA AAACCCACGC CGCTCATGTT ATGCCTTCTT CTTTTTCCTA CAGCTCCTGG 61 GCAACGTGCT GGTTATTGTG CTGTCTCATC ATTTTGGCAA AGAATTGATT TATCGATTTG 121 CAGACCGTGC ATCATGAGCC AGTTTGATAT CCTGTGCAAG ACACCACCTA AGGTGCTGGT 181 GCGCCAGTTC GTGGAGAGGT TTGAGAGGCC CTCTGGAGAG AAGATTGCCT CCTGTGCAGC 241 TGAGCTGACC TACCTGTGCT GGATGATCAC CCACAACGGC ACAGCCATCA AGAGGGCCAC 301 CTTTATGAGC TACAACACCA TCATTAGCAA CTCCCTGAGC TTCGACATTG TGAACAAGTC 361 CCTCCAGTTT AAATACAAGA CCCAGAAGGC CACAATCCTG GAGGCCTCCC TGAAGAAATT 421 GATTCCTGCT TGGGAGTTCA CCATCATCCC CTACAATGGC CAGAAGCACC AGTCTGATAT 481 CACTGATATT GTGAGCAGTC TGCAACTCCA GTTCGAGTCC TCTGAGGAAG CTGACAAGGG 541 CAACAGCCAC AGCAAGAAGA TGCTGAAGGC CCTGCTCAGT GAGGGAGAAA GCATCTGGGA 601 GATCACTGAG AAGATCCTGA ACTCCTTTGA GTACACTTCC AGATTCACCA AGACCAAGAC 661 CTTGTACCAG TTCCTGTTCC TGGCCACCTT CATCAACTGT GGCAGGTTCA GCGACATCAA 721 GAATGTGGAT CCCAAATCCT TTAAACTGGT CCAGAACAAG TACCTGGGAG TGATCATCCA 781 GTGCCTGGTG ACAGAGACCA AGACCTCTGT GAGCAGGCAC ATCTACTTCT TCTCTGCCAG 841 GGGCAGGATT GATCCACTGG TGTACCTGGA TGAGTTCCTG AGGAACTCTG AGCCAGTGCT 901 GAAGCGGGTG AACAGGACCG GCAACTCTTC CAGCAACAAG CAGGAGTACC AGCTGCTCAA 961 AGGACAACCT GGTGAGGTCC TACAACAAAG CTTTGAAGAA AAATGCCCCC CTACCCAATC 1021 TTTGCCATCA AGAATGGGCC CTAAGTCCCA CATTTGGCAG ACACCTGATG ACCTCGTTCT 1081 GTCCATGAAA GGCCCTGAAC GAGCCTGAAC AATGGATGTG GGCCAACTGG AGCCGGAATA 1141 GCCGGGGGCC CTCTCTGGCC CGT // |
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| Primer: pAxCA_R1 | RDB13038_A4Lgb.seq |
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>07083_13038_A4Lg_pAxCA_R1_G03_21.ab1 1 CAAGGGACCC AGCCGCCCTT CGCCACACCA GCCCACCACC TTCTGAATAG GCAGCCCTGC 61 ACCTTGAGGA GTGAATTGAT TATCGATTCT AGACTAGTTT ATAATTTGTA CCTCTAGAGT 121 CAGATGCGTC TGTTGATGTA GGAAGACAGG TAGTCCAGCA CCTCCTGGGA AATGATCCCA 181 TTCCAGGCAG GGTAGCGAAT GCTGCCCTCG GCACTACCCT TCAGCTGCTC AATGTGCTGC 241 CACTCCTCAA TTGGGTTGGT CTCATCCTTC AATGCAATCA TCTCCTTGGA GATGGGATCA 301 TATGCATAGT ACCGAGACAC CAGTGCGAAG TAGTGATCAG GGATTGCTGT GATCTGGTGA 361 GTATAGGTTG TTCTGGCCAC GGCAGAGGCC CGCTTATCGC TCCAGTTGCC CACAACATTG 421 GTCAGCTCTG TCAGGCCCTT CATGGACAGG AAGGAGGTCA TCAGGTGTCT GCCAATGTGG 481 GACTTAGGGC CATTCTTGAT GGCAAAGATT GGGTAGGGGG CATTTTTCTT CAAAGCTTTG 541 TTGTAGGACC TCACCAGGTT GTCCTTGAGC AGCTGGTACT CCTGCTTGTT GCTGGAAGAG 601 TTGCCGGTCC TGTTCACCCG CTTCAGCACT GGCTCAGAGT TCCTCAGGAA CTCATCCAGG 661 TACACCCAGT GGATCAATCC TGCCCCTGGC AGAGAAGAAG TAGATGTGCC TGCTCACAGA 721 GGTCTTGGTC TCTGTCACCA GGCACTGGAT GATCACTCCC AGGTACTTGT TCTGGACCAG 781 TTTAAAAGGA TTTGGGATCC ACATTCTTGA TGTCGCTGAA CCCTGCCACA GTTGATGAAA 841 GGTGGCCAGA ACAGGAACTG GTACAAGGTC TTGGGTCCTT GGTGAATCTG GAATGTACTC 901 AAAGAGTTCA AGATCTTCTT CAGTGATCTC CCAGATGCTT TTCTCCCTCA CTGAACC // |
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Please visit Sequencing and PCR primers for primer information.
References
| original | Kondo, S., Activities of various FLP recombinases expressed by adenovirus vectors in mammalian cells. J. Mol. Biol., 390, 221-230 (2009). PMID 19414019. |
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2018.12.10
GNP_filter3_RDBDEP_html_181012.pl