Resource data sheet
Prev.

mouse SDF-1 alpha cDNA

Plasmid clone of mouse SDF-1 alpha cDNA

Catalog number RDB06778
Resource name mouse SDF-1 alpha cDNA
Alternative name #8852
Clone info. Plasmid clone of mouse SDF-1 alpha cDNA.
Vector backbone pBluescript SK(-) (Plasmid)
Selectable markers Amp^r
Gene/insert name Mouse stromal cell-derived factor 1; SDF1 cDNA
Depositor Honjo, Tasuku |
 
Other clones in our bank mouse Cxcl12 (NCBI Gene 20315) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Science, 261, 600-603 (1993).). The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Kyoto University Medical Science and Business Liaison Organization Liaison Office (contracts@kumbl.med.kyoto-u.ac.jp). When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the name of resource, RECIPIENT's name and his/her affiliation, purpose of the use, and number of resources.
Ordering Please visit Information of Request for Distribution. 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06778 mouse SDF-1 alpha cDNA DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Tashiro, K., Signal sequence trap: a cloning strategy for secreted proteins and type I membrane proteins. Science, 261, 600-603 (1993). PMID 8342023.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12810_A4Fi.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 RDB12810_A4Fia.seq
>06778_12810_A4Fi_M13_-40_G02_20.ab1
    1 CGGCAGGTAA TTGTATACGA CTCACTATAG GGCGAATTGG GTACCGGGCC CCCCCTCGAG
   61 GTCGACCCAC GCGTCCGGAC CACTTTCCCT CTCGGTCCAC CTCGGTGTCC TCTTGCTGTC
  121 CAGCTCTGCA GCCTCCGGCG CGCCCTCCCG CCCACGCCAT GGACGCCAAG GTCGTCGCCG
  181 TGCTGGCCCT GGTGCTGGCC GCGCTCTGCA TCAGTGACGG TAAACCAGTC AGCCTGAGCT
  241 ACCGATGCCC CTGCCGGTTC TTCGAGAGCC ACATCGCCAG AGCCAACGTC AAGCATCTGA
  301 AAATCCTCAA CACTCCAAAC TGTGCCCTTC AGATTGTTGC ACGGCTGAAG AACAACAACA
  361 GACAAGTGTG CATTGACCCG AAATTAAAGT GGATCCAAGA GTACCTGGAG AAAGCTTTAA
  421 ACAAGTAAGC ACAACAGCCC AAAGGACTTT CCAGTAGACC CCCGAGGAAG GCTGACATCC
  481 GTGGGAGATG CAAGGGCAGT GGTGGGGAGG AGGGCCTGAA CCCTGGCCAG GATGGCCGGC
  541 GGGACAGCAC TGACTGGGGT CATGCTAAGG TTTGCCAGCA TAAAGACACT CCGCCATAGC
  601 ATATGGTACG ATATTGCAGC TTATATTCAT CCCTGCCCTC GCCCGTGCAC AATGGAGCTT
  661 TTATAACTGG GGTTTTTCTA AGGAATTGTA TTACCCTAAC CAGTTAGCTT CATCCCCATT
  721 CTCCTCATCC TCATCTTCAT TTTAAAAAGC AGTGATTACT TCAAGGGCTG TATTCAGTTT
  781 GCTTTGGAGC TTCTCTTTGC CCTGGGGCCT CTGGGCACAG TTATAGACGG TGGCTTTGCA
  841 GGGAGCCCTA GAGAGAAACC TTCCACCAGA GCAGAGTCCG AGGAACGCTG CAGGGCTTGT
  901 CCTGCAGGGG GCGCTCCTCG ACAGATGCCT TGTCCTGAGT CAACACAAGA TCCGGCAGAG
  961 GGAGGCTCCT TTATCCAGTT CAGTGCCAGG GTCGGGAAAG CTTCCCTTTA GAAAGTGATC
 1021 CCTGAAGCTG TGCTCAGAGA CCCTTTCCTA GCCCGTTTCC TGCTCTCTGC TGCTCGAACG
 1081 CATGCTTCAT CTGAACTCCG GCTCTCACTC CTGTAGCCTG ACGGACCAAT GCCTGGCAAT
1141 G
//
Primer: Reverse2 RDB12810_A4Fib.seq
>06778_12810_A4Fi_Reverse2_H02_23.ab1
    1 CGTCCTGATT ACGCCAGCTC GAATTAACCC TCACTAAAGG GAACAAAAGC TGGAGCTCCA
   61 CCGCGGTGGC GGCCGCCCTT TTTTTTTTTT TTTTACAGCA TGAAACAATT AGCATTTTAT
  121 TGCTAGTGCA TATAATGTCA CAGTTGATAC AATTTCATTC CAAGTGGAAA AATACACCGT
  181 GGCTGACACC GGAAAGCTAC AATACATTTA TATGTCATAT ATATTTCTTT ACAAGTTGCC
  241 AGTAGCTCAA GATCATAGAG ATATCTACTG GCATTACTAT GGCTCCACTT CAAATATATG
  301 AATTGACTAC AAATATATTC TGAAATACAT TTGTCTTCCA AAGAAACATA AAAAGTGCAC
  361 AAAAATATAT GTAAAAAAAT GCCTTGTAAA TAGTTACCAA AAACCACCAG GGCCGTCTGT
  421 GATCATAAGA ATGTATGCAA GTTTTATCTT GTCTCCATTT CTGATTGGAA CCCAGAATCC
  481 CCACTGTGGC TTCATGGCAA GATTCTGGCT TATTCGTTAT TATTATTATT ATTATTATTT
  541 TCATCACTGA TATTCGATCA GAGCCCATAG AGCCACTGTT GAAATATATA GGACTCAGGG
  601 ACAATTACCA AGGTCCAAAT CCTCATTTAA ACTTCCGTTT ATGATTAATT CTCAGACTTG
  661 TGTTTTGTGG GTAAATCTAG GCATGTTCTC ACATTATCCA GGATGGTCTG GCTCCATTCT
  721 ACAGGAGGCC AAAGAAAAAT CTCACAAAGG GCCCAAGTGA GAGGAAAGCA AAGGGAAGAG
  781 AGGGAGGGGG CACCCCACAT CACTCTCCTC CCTTCCATTG CAGCATTGGT CCGTCAGGCT
  841 ACAGAGGTGA GAAGCGGAAG TCAGATGAAG CATGCGTTTG GAGGCAAGCA GAGAGCAGGA
  901 ACGGCTAGGA AAGGGTCTCT GAGCACAGCT TCAGGGATCA CTTCTAAAGG AAGCTTCCCG
  961 ACCCTGGCAC TGAACTGGAT AAAGGGAGCC CTCCCTCTGC CGGATCTTTG TGTTGACTCA
 1021 GGACAAGGGC ATCTGTCGAG GGAGCGCCCC CCTGCAGGAC AAGCCCCTGC AGCGTTTCCT
 1081 TCGGACTCTG CTCTGGTGGA TGGATACTCT CTAGGCTTCC CTTGCCAAGT CACCGGATCT
1141 ATAAACTGGT GCCCAGAGAG AGGG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Tashiro, K., Signal sequence trap: a cloning strategy for secreted proteins and type I membrane proteins. Science, 261, 600-603 (1993). PMID 8342023.

2019.02.05

GNP_filter3_RDBDEP_html_190131.pl