Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

mouse SDF-1 alpha cDNA (#RDB06778)

Plasmid clone of mouse SDF-1 alpha cDNA

Alternative name #8852
Clone info. Plasmid clone of mouse SDF-1 alpha cDNA.
Vector backbone pBluescript SK(-) (Plasmid)
Selectable markers Amp^r
Gene/insert name Mouse stromal cell-derived factor 1; SDF1 cDNA
Depositor|Developer Honjo, Tasuku |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Science, 261, 600-603 (1993).). The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Kyoto University Medical Science and Business Liaison Organization Liaison Office (contracts@kumbl.med.kyoto-u.ac.jp). When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the name of resource, RECIPIENT's name and his/her affiliation, purpose of the use, and number of resources.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Science, 261, 600-603 (1993).)。 公表を前提とした学術研究に限る。営利機関の利用者は、事前に京都大学・「医学領域」産学連携推進機構・知財室 (contracts@kumbl.med.kyoto-u.ac.jp) に連絡し、承諾を得ること。理研BRCは、本件リソースのリソース名、提供先、利用目的、件数を京都大学・本庶佑教授に報告する。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06778 mouse SDF-1 alpha cDNA DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The mouse SDF-1 alpha cDNA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06778).

Reference section:

Tashiro, K., Tada, H., Heilker, R., Shirozu, M., Nakano, T., Honjo, T., Signal sequence trap: a cloning strategy for secreted proteins and type I membrane proteins. Science, 261, 600-603 (1993). PMID 8342023. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12810_A4Fi.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40
Sequence file: RDB12810_A4Fia.seq check
>06778_12810_A4Fi_M13_-40_G02_20.ab1
    1 CGGCAGGTAA TTGTATACGA CTCACTATAG GGCGAATTGG GTACCGGGCC CCCCCTCGAG
   61 GTCGACCCAC GCGTCCGGAC CACTTTCCCT CTCGGTCCAC CTCGGTGTCC TCTTGCTGTC
  121 CAGCTCTGCA GCCTCCGGCG CGCCCTCCCG CCCACGCCAT GGACGCCAAG GTCGTCGCCG
  181 TGCTGGCCCT GGTGCTGGCC GCGCTCTGCA TCAGTGACGG TAAACCAGTC AGCCTGAGCT
  241 ACCGATGCCC CTGCCGGTTC TTCGAGAGCC ACATCGCCAG AGCCAACGTC AAGCATCTGA
  301 AAATCCTCAA CACTCCAAAC TGTGCCCTTC AGATTGTTGC ACGGCTGAAG AACAACAACA
  361 GACAAGTGTG CATTGACCCG AAATTAAAGT GGATCCAAGA GTACCTGGAG AAAGCTTTAA
  421 ACAAGTAAGC ACAACAGCCC AAAGGACTTT CCAGTAGACC CCCGAGGAAG GCTGACATCC
  481 GTGGGAGATG CAAGGGCAGT GGTGGGGAGG AGGGCCTGAA CCCTGGCCAG GATGGCCGGC
  541 GGGACAGCAC TGACTGGGGT CATGCTAAGG TTTGCCAGCA TAAAGACACT CCGCCATAGC
  601 ATATGGTACG ATATTGCAGC TTATATTCAT CCCTGCCCTC GCCCGTGCAC AATGGAGCTT
  661 TTATAACTGG GGTTTTTCTA AGGAATTGTA TTACCCTAAC CAGTTAGCTT CATCCCCATT
  721 CTCCTCATCC TCATCTTCAT TTTAAAAAGC AGTGATTACT TCAAGGGCTG TATTCAGTTT
  781 GCTTTGGAGC TTCTCTTTGC CCTGGGGCCT CTGGGCACAG TTATAGACGG TGGCTTTGCA
  841 GGGAGCCCTA GAGAGAAACC TTCCACCAGA GCAGAGTCCG AGGAACGCTG CAGGGCTTGT
  901 CCTGCAGGGG GCGCTCCTCG ACAGATGCCT TGTCCTGAGT CAACACAAGA TCCGGCAGAG
  961 GGAGGCTCCT TTATCCAGTT CAGTGCCAGG GTCGGGAAAG CTTCCCTTTA GAAAGTGATC
 1021 CCTGAAGCTG TGCTCAGAGA CCCTTTCCTA GCCCGTTTCC TGCTCTCTGC TGCTCGAACG
 1081 CATGCTTCAT CTGAACTCCG GCTCTCACTC CTGTAGCCTG ACGGACCAAT GCCTGGCAAT
1141 G
//
Primer: Reverse2
Sequence file: RDB12810_A4Fib.seq check
>06778_12810_A4Fi_Reverse2_H02_23.ab1
    1 CGTCCTGATT ACGCCAGCTC GAATTAACCC TCACTAAAGG GAACAAAAGC TGGAGCTCCA
   61 CCGCGGTGGC GGCCGCCCTT TTTTTTTTTT TTTTACAGCA TGAAACAATT AGCATTTTAT
  121 TGCTAGTGCA TATAATGTCA CAGTTGATAC AATTTCATTC CAAGTGGAAA AATACACCGT
  181 GGCTGACACC GGAAAGCTAC AATACATTTA TATGTCATAT ATATTTCTTT ACAAGTTGCC
  241 AGTAGCTCAA GATCATAGAG ATATCTACTG GCATTACTAT GGCTCCACTT CAAATATATG
  301 AATTGACTAC AAATATATTC TGAAATACAT TTGTCTTCCA AAGAAACATA AAAAGTGCAC
  361 AAAAATATAT GTAAAAAAAT GCCTTGTAAA TAGTTACCAA AAACCACCAG GGCCGTCTGT
  421 GATCATAAGA ATGTATGCAA GTTTTATCTT GTCTCCATTT CTGATTGGAA CCCAGAATCC
  481 CCACTGTGGC TTCATGGCAA GATTCTGGCT TATTCGTTAT TATTATTATT ATTATTATTT
  541 TCATCACTGA TATTCGATCA GAGCCCATAG AGCCACTGTT GAAATATATA GGACTCAGGG
  601 ACAATTACCA AGGTCCAAAT CCTCATTTAA ACTTCCGTTT ATGATTAATT CTCAGACTTG
  661 TGTTTTGTGG GTAAATCTAG GCATGTTCTC ACATTATCCA GGATGGTCTG GCTCCATTCT
  721 ACAGGAGGCC AAAGAAAAAT CTCACAAAGG GCCCAAGTGA GAGGAAAGCA AAGGGAAGAG
  781 AGGGAGGGGG CACCCCACAT CACTCTCCTC CCTTCCATTG CAGCATTGGT CCGTCAGGCT
  841 ACAGAGGTGA GAAGCGGAAG TCAGATGAAG CATGCGTTTG GAGGCAAGCA GAGAGCAGGA
  901 ACGGCTAGGA AAGGGTCTCT GAGCACAGCT TCAGGGATCA CTTCTAAAGG AAGCTTCCCG
  961 ACCCTGGCAC TGAACTGGAT AAAGGGAGCC CTCCCTCTGC CGGATCTTTG TGTTGACTCA
 1021 GGACAAGGGC ATCTGTCGAG GGAGCGCCCC CCTGCAGGAC AAGCCCCTGC AGCGTTTCCT
 1081 TCGGACTCTG CTCTGGTGGA TGGATACTCT CTAGGCTTCC CTTGCCAAGT CACCGGATCT
1141 ATAAACTGGT GCCCAGAGAG AGGG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Tashiro, K., Signal sequence trap: a cloning strategy for secreted proteins and type I membrane proteins. Science, 261, 600-603 (1993). PMID 8342023. [link to RRC of NBRP]