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hPrP129M in pTA2

Plasmid clone of human prion cDNA

Catalog number RDB06716
Resource name hPrP129M in pTA2
Clone info. Plasmid clone of human prion cDNA. There is polymorphism at codon 129. This clone has Met at codon 129. Nucleotide sequence of 75 to 700 of AB300823.1 was cloned. Signal peptide and propeptide are not included.
Vector backbone pTA2 (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name Human hPrP 129M cDNA
Reference of nucleotide sequence AB300823 (DDBJ accession number)
Depositor|Developer Nakajima, Osamu | Kikuchi, Yutaka |
 
Other clones in our bank human PRNP (NCBI Gene 5621) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (FEBS J., 275, 2965-2976 (2008))
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RDB06716 hPrP129M in pTA2 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Kikuchi, Y., Hypoxia induces expression of a GPI-anchorless splice variant of the prion protein. FEBS J., 275, 2965-2976 (2008). PMID 18445040.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB06716_A3Jh.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer:
Sequence file: RDB06716_A3Jha.seq check
>06716_06716_A3Jh_3_T7_20bp_D02_11.ab1
    1 TACGTGGGAC CCCTCGAGGT CGACGGTATC GATAAGCTTG ATATCGAATT CCCAATACTC
   61 GGAATTCCAT ATGAGCAAGA AGCGCCCGAA GCCTGGAGGA TGGAACACTG GGGGCAGCCG
  121 ATACCCGGGG CAGGGCAGCC CTGGAGGCAA CCGCTACCCA CCTCAGGGCG GTGGTGGCTG
  181 GGGGCAGCCT CATGGTGGTG GCTGGGGGCA GCCTCATGGT GGTGGCTGGG GGCAGCCCCA
  241 TGGTGGTGGC TGGGGACAGC CTCATGGTGG TGGCTGGGGT CAAGGAGGTG GCACCCACAG
  301 TCAGTGGAAC AAGCCGAGTA AGCCAAAAAC CAACATGAAG CACATGGCTG GTGCTGCAGC
  361 AGCTGGGGCA GTGGTGGGGG GCCTTGGCGG CTACATGCTG GGAAGTGCCA TGAGCAGGCC
  421 CATCATACAT TTCGGCAGTG ACTATGAGGA CCGTTACTAT CGTGAAAACA TGCACCGTTA
  481 CCCCAACCAA GTGTACTACA GGCCCATGGA TGAGTACAGC AACCAGAACA ACTTTGTGCA
  541 CGACTGCGTC AATATCACAA TCAAGCAGCA CACGGTCACC ACAACCACCA AGGGGGAGAA
  601 CTTCACCGAG ACCGACGTTA AGATGATGGA GCGCGTGGTT GAGCAGATGT GTATCACCCA
661 GTACGAGAGG GAATC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kikuchi, Y., Hypoxia induces expression of a GPI-anchorless splice variant of the prion protein. FEBS J., 275, 2965-2976 (2008). PMID 18445040.

2021.03.25

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