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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pFFH41

S. pombe expression vector with nmt1 promoter

Catalog number RDB06587
Resource name pFFH41
Clone info. S. pombe expression vector with nmt1 promoter
Vector backbone pREP41 (Plasmid)
Selectable markers Amp^r
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
            Ordering Please visit Information of Request for Distribution.[link] 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、 寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB06587 pFFH41 DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB06587z.seq

            Original reference

            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB14498_A6L7p1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: nmt1Fw
            Sequence file: RDB14498_A6L7a.seq check
            >06587_14498_A6L7_nmt1Fw_A10_01_ABI24.ab1
                1 TGGAATTTAA TTAAATCAGG AAAACGTAAC TCTCGGCTAC TGGATGGTTC AGTCACCCAA
               61 CGATTACTGG GGAGAGAAAA CAGGGCAAAA GCAAAGCTTA AAGGAATCCG ATTGTCATTC
              121 GGCAATGTGC AGCGAAACTA AAAACCGGAT AATGGACCTG TTAATCGAAA CATTGAAGAT
              181 AAAGGAAGAG GAATCCTGGC ATATCATCAA TTGAATAAGT TGAATTAATT ATTTCAATCT
              241 CATTCTCACT TTCTGACTTA TAGTCGCTTT GTTAAAGCTA GCGTCGACAG GCCTGGATCC
              301 GGATATCATG GATTATAAGG ATGATGACGA TAAACCCATG GACTACAAGG ACGACGATGA
              361 CAAACATCAC CACCATCACC ACTGAGATCT CGAGCCCGGG CGGCCGCTAA CTAACTAAGG
              421 GTAAAAGGAA TGTCTCCCTT GCCAGTACTG CTAGGGTTTT TCTTTCAAAC TATGGAAGCC
              481 CATTCAAGCT GCATATTACG ATTTTGTTTT TCGCTTTTAG AAAGTGGTTT AGATGAGATA
              541 ATAGAAAAAT TCTTGATCTC CGACAACGAG TACTTTTATT TTTTTTGCTA ATCACTTTAC
              601 TCAATATTAG CTCGAAATCG TAGAAACGTA GACGGGTGCG GGATACCGAG TGGTGTAGTT
              661 AAGAATTTTT ATAAACCACG TGGCCCAAAA ATATGAACCC AAAACGTTTA TACATGAGTA
              721 TACTTTAAGA AGGCTATACC CCTTCGTGTT AGATGTAGTT TTAGCTACCC AACCCGAGTC
              781 TATGAGCTTG ACTTCAGATG TAGAAGGCAT TAAATCGTTT TGAATATTAA TTAAAAAACG
              841 ATGAAAATTA AATATTTAAA AGCAATCATA CGCTGAAAAT TTAGTGCTGT GGCTAATCCT
              901 TCAACATGGA AATGCCATAA AAGTGACTTT GACAAAAAAA AAAAAGTATA TACAGGGTAG
              961 TAAACTCATC TACTTCATTT GACTTTGTTT ACAGCATGTG GAAGGAAGAA TATTTATTTG
             1021 CTAAATTCGT TAGTTTTACC ATTTCCAATA AGTAATACCT AATGAAATCC GACAGATTGG
            1081 CGCATGATGA AAAGAGCCAT TTTGCTTTTG GGGAAGAAAC TG
            //
            Primer: f1ori_R3
            Sequence file: RDB14498_A6L7b.seq check
            >06587_14498_A6L7_f1ori_R3_C10_07_ABI24.ab1
                1 GCCAGAGGGG CGTTGAGTAT GCGGGTGTGA ATACCGCACA GATGCGTAAG GAGAAAATAC
               61 CGCATCAGGC GCCATTCGCC ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCGG
              121 GCCTCTTCGC TATTACGCCA GCTGGCGAAA GGGGGATGTG CTGCAAGGCG ATTAAGTTGG
              181 GTAACGCCAG GGTTTTCCCA GTCACGACGT TGTAAAACGA CGGCCAGTGA ATTCCAACCT
              241 TCCAATTCAT TAAATCGAGT CCTCCATCAG ATGATTTACA AGGTTTTGCA TAGAATCCCT
              301 AAGTAAGAGA AGTTGTTAGT GATTTCTTCA AGTTTACTAA AATTTACATA CAAATGGATG
              361 ATCTCTTCTC CATTGTTTCC TTTCTTCTTG CAAACGCGTT TTACAAAGAG ATGACATGCT
              421 ATTTTCTGAT TATTTTTTTC TATAGTTTTC TATTTTGTAC GTCCTTGGGA GTCCGAAATG
              481 TAAAATCGGG GTATGGTTAG TAGCGGCAGC GTTCATTATG GTATATAAAA GATAAAAAGT
              541 AGGGGCAAGC GAGACAGAAT GGGATACAAG GGCATCGTCT ATAATTATAG CTAAAAATTG
              601 TATTTTAATT TGTATTTTTT GTAATTTTAT TTCTCATTGT TTTACTTAAA ATGAATGCGA
              661 ATTAGAGAAA ACTTCAACGA AATGTCAAAA TAAGCTCAGC AAAATATACA ATTTTAGGGA
              721 AAGCGATCAG CAACTTATTC TCCGTTGTAG ATTCTTTTTC TTCATAAATT CCAACAATGT
              781 AACTGCCAAT TGATTTTTCT CTAGATGCTA ATAAATTGAA ACTAATGAAT ATAATAACAC
              841 AGGTTTTGAT AAATTGTAAA CTCTTACTCA AAAAAAAGAA TGTTAAAAAC TAATTTAACC
              901 TTTTTATAAA GGCTCATGGG CGTTTAAAGA GGATAGTAAA AATTCTACAA TTTTTTAATT
              961 TAAATGTCAC AATTGTCTTG CGAATATAAT TCTAATTAAT TTTTCGCTTA ACTGCTTGTT
             1021 TAGTACTATA CTTTCAATAT GCGTTATATG CAACCTTTAT CCATGGAGAT TGCGATATTT
            1081 TTGGCAATCT ATCCTGGTAC CTTGGGAGGC GGCGTTCTAA CTCAAC
            //
            Primer: LEU2pro_F
            Sequence file: RDB14498_A6L7c.seq check
            >06587_14498_A6L7_LEU2pro_F_B10_04_ABI24.ab1
                1 AATCCGGCAG TTGAATATTA TTTAAGGACC TATTGTTTTT TCCAATAGGT GGTTAGCAAT
               61 CGTCTTACTT TCTAACTTTT CTTACCTTTT ACATTTCAGC AATATATATA TATATTTCAA
              121 GGATATACCA TTCTAATGTC TGCCCCTAAG AAGATCGTCG TTTTGCCAGG TGACCACGTT
              181 GGTCAAGAAA TCACAGCCGA AGCCATTAAG GTTCTTAAAG CTATTTCTGA TGTTCGTTCC
              241 AATGTCAAGT TCGATTTCGA AAATCATTTA ATTGGTGGTG CTGCTATCGA TGCTACAGGT
              301 GTTCCACTTC CAGATGAGGC GCTGGAAGCC TCCAAGAAGG CTGATGCCGT TTTGTTAGGT
              361 GCTGTGGGTG GTCCTAAATG GGGTACCGGT AGTGTTAGAC CTGAACAAGG TTTACTAAAA
              421 ATCCGTAAAG AACTTCAATT GTACGCCAAC TTAAGACCAT GTAACTTTGC ATCCGACTCT
              481 CTTTTAGACT TATCTCCAAT CAAGCCACAA TTTGCTAAAG GTACTGACTT CGTTGTTGTC
              541 AGAGAATTAG TGGGAGGTAT TTACTTTGGT AAGAGAAAGG AAGACGATGG TGATGGTGTC
              601 GCTTGGGATA GTGAACAATA CACCGTTCCA GAAGTGCAAA GAATCACAAG AATGGCCGCT
              661 TTCATGGCCC TACAACATGA GCCACCATTG CCTATTTGGT CCTTGGATAA AGCTAATGTT
              721 TTGGCCTCTT CAAGATTATG GAGAAAAACT GTGGAGGAAA CCATCAAGAA CGAATTCCCT
              781 ACATTGAAGG TTCAACATCA ATTGATTGAT TCTGCCGCCA TGATCCTAGT TAAGAACCCA
              841 ACCCACCTAA ATGGTATTAT AATCACCAGC AACATGTTTG GTGACATCAT CTCCGATGAA
              901 GCCCTCCGTT ATCCCAGGTT TCCTTGGGTT TGTTGCCATC TGCGTCCTTG GCCTCTTTGC
              961 CAGACAAGAA CACCGCATTT GGTTTGTACG AACCCATGCA CGTTCTGCTC CAGATTTGCC
             1021 AAAGATAAGT CCACCCTATC GCACTATCTG GTCTGCCTGC ATGAATGTGA ATGTCATTGA
            1081 ACTTGCTGGA GAGGTAAGGC ATTGG
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

            2022.02.09

            GNP_filter3_RDBDEP_html_220112.pl