Resource data sheet
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pFFH41

S. pombe expression vector with nmt1 promoter

Catalog number RDB06587
Resource name pFFH41
Clone info. S. pombe expression vector with nmt1 promoter
Vector backbone pREP41 (Plasmid)
Selectable markers Amp^r
Depositor Yoshida, Minoru | Matsuyama, Akihisa |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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RDB06587 pFFH41 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB06587z.seq from Depositor

Featured content

Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

References

original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14498_A6L7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw RDB14498_A6L7a.seq
>06587_14498_A6L7_nmt1Fw_A10_01_ABI24.ab1
    1 TGGAATTTAA TTAAATCAGG AAAACGTAAC TCTCGGCTAC TGGATGGTTC AGTCACCCAA
   61 CGATTACTGG GGAGAGAAAA CAGGGCAAAA GCAAAGCTTA AAGGAATCCG ATTGTCATTC
  121 GGCAATGTGC AGCGAAACTA AAAACCGGAT AATGGACCTG TTAATCGAAA CATTGAAGAT
  181 AAAGGAAGAG GAATCCTGGC ATATCATCAA TTGAATAAGT TGAATTAATT ATTTCAATCT
  241 CATTCTCACT TTCTGACTTA TAGTCGCTTT GTTAAAGCTA GCGTCGACAG GCCTGGATCC
  301 GGATATCATG GATTATAAGG ATGATGACGA TAAACCCATG GACTACAAGG ACGACGATGA
  361 CAAACATCAC CACCATCACC ACTGAGATCT CGAGCCCGGG CGGCCGCTAA CTAACTAAGG
  421 GTAAAAGGAA TGTCTCCCTT GCCAGTACTG CTAGGGTTTT TCTTTCAAAC TATGGAAGCC
  481 CATTCAAGCT GCATATTACG ATTTTGTTTT TCGCTTTTAG AAAGTGGTTT AGATGAGATA
  541 ATAGAAAAAT TCTTGATCTC CGACAACGAG TACTTTTATT TTTTTTGCTA ATCACTTTAC
  601 TCAATATTAG CTCGAAATCG TAGAAACGTA GACGGGTGCG GGATACCGAG TGGTGTAGTT
  661 AAGAATTTTT ATAAACCACG TGGCCCAAAA ATATGAACCC AAAACGTTTA TACATGAGTA
  721 TACTTTAAGA AGGCTATACC CCTTCGTGTT AGATGTAGTT TTAGCTACCC AACCCGAGTC
  781 TATGAGCTTG ACTTCAGATG TAGAAGGCAT TAAATCGTTT TGAATATTAA TTAAAAAACG
  841 ATGAAAATTA AATATTTAAA AGCAATCATA CGCTGAAAAT TTAGTGCTGT GGCTAATCCT
  901 TCAACATGGA AATGCCATAA AAGTGACTTT GACAAAAAAA AAAAAGTATA TACAGGGTAG
  961 TAAACTCATC TACTTCATTT GACTTTGTTT ACAGCATGTG GAAGGAAGAA TATTTATTTG
 1021 CTAAATTCGT TAGTTTTACC ATTTCCAATA AGTAATACCT AATGAAATCC GACAGATTGG
1081 CGCATGATGA AAAGAGCCAT TTTGCTTTTG GGGAAGAAAC TG
//
Primer: f1ori_R3 RDB14498_A6L7b.seq
>06587_14498_A6L7_f1ori_R3_C10_07_ABI24.ab1
    1 GCCAGAGGGG CGTTGAGTAT GCGGGTGTGA ATACCGCACA GATGCGTAAG GAGAAAATAC
   61 CGCATCAGGC GCCATTCGCC ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCGG
  121 GCCTCTTCGC TATTACGCCA GCTGGCGAAA GGGGGATGTG CTGCAAGGCG ATTAAGTTGG
  181 GTAACGCCAG GGTTTTCCCA GTCACGACGT TGTAAAACGA CGGCCAGTGA ATTCCAACCT
  241 TCCAATTCAT TAAATCGAGT CCTCCATCAG ATGATTTACA AGGTTTTGCA TAGAATCCCT
  301 AAGTAAGAGA AGTTGTTAGT GATTTCTTCA AGTTTACTAA AATTTACATA CAAATGGATG
  361 ATCTCTTCTC CATTGTTTCC TTTCTTCTTG CAAACGCGTT TTACAAAGAG ATGACATGCT
  421 ATTTTCTGAT TATTTTTTTC TATAGTTTTC TATTTTGTAC GTCCTTGGGA GTCCGAAATG
  481 TAAAATCGGG GTATGGTTAG TAGCGGCAGC GTTCATTATG GTATATAAAA GATAAAAAGT
  541 AGGGGCAAGC GAGACAGAAT GGGATACAAG GGCATCGTCT ATAATTATAG CTAAAAATTG
  601 TATTTTAATT TGTATTTTTT GTAATTTTAT TTCTCATTGT TTTACTTAAA ATGAATGCGA
  661 ATTAGAGAAA ACTTCAACGA AATGTCAAAA TAAGCTCAGC AAAATATACA ATTTTAGGGA
  721 AAGCGATCAG CAACTTATTC TCCGTTGTAG ATTCTTTTTC TTCATAAATT CCAACAATGT
  781 AACTGCCAAT TGATTTTTCT CTAGATGCTA ATAAATTGAA ACTAATGAAT ATAATAACAC
  841 AGGTTTTGAT AAATTGTAAA CTCTTACTCA AAAAAAAGAA TGTTAAAAAC TAATTTAACC
  901 TTTTTATAAA GGCTCATGGG CGTTTAAAGA GGATAGTAAA AATTCTACAA TTTTTTAATT
  961 TAAATGTCAC AATTGTCTTG CGAATATAAT TCTAATTAAT TTTTCGCTTA ACTGCTTGTT
 1021 TAGTACTATA CTTTCAATAT GCGTTATATG CAACCTTTAT CCATGGAGAT TGCGATATTT
1081 TTGGCAATCT ATCCTGGTAC CTTGGGAGGC GGCGTTCTAA CTCAAC
//
Primer: LEU2pro_F RDB14498_A6L7c.seq
>06587_14498_A6L7_LEU2pro_F_B10_04_ABI24.ab1
    1 AATCCGGCAG TTGAATATTA TTTAAGGACC TATTGTTTTT TCCAATAGGT GGTTAGCAAT
   61 CGTCTTACTT TCTAACTTTT CTTACCTTTT ACATTTCAGC AATATATATA TATATTTCAA
  121 GGATATACCA TTCTAATGTC TGCCCCTAAG AAGATCGTCG TTTTGCCAGG TGACCACGTT
  181 GGTCAAGAAA TCACAGCCGA AGCCATTAAG GTTCTTAAAG CTATTTCTGA TGTTCGTTCC
  241 AATGTCAAGT TCGATTTCGA AAATCATTTA ATTGGTGGTG CTGCTATCGA TGCTACAGGT
  301 GTTCCACTTC CAGATGAGGC GCTGGAAGCC TCCAAGAAGG CTGATGCCGT TTTGTTAGGT
  361 GCTGTGGGTG GTCCTAAATG GGGTACCGGT AGTGTTAGAC CTGAACAAGG TTTACTAAAA
  421 ATCCGTAAAG AACTTCAATT GTACGCCAAC TTAAGACCAT GTAACTTTGC ATCCGACTCT
  481 CTTTTAGACT TATCTCCAAT CAAGCCACAA TTTGCTAAAG GTACTGACTT CGTTGTTGTC
  541 AGAGAATTAG TGGGAGGTAT TTACTTTGGT AAGAGAAAGG AAGACGATGG TGATGGTGTC
  601 GCTTGGGATA GTGAACAATA CACCGTTCCA GAAGTGCAAA GAATCACAAG AATGGCCGCT
  661 TTCATGGCCC TACAACATGA GCCACCATTG CCTATTTGGT CCTTGGATAA AGCTAATGTT
  721 TTGGCCTCTT CAAGATTATG GAGAAAAACT GTGGAGGAAA CCATCAAGAA CGAATTCCCT
  781 ACATTGAAGG TTCAACATCA ATTGATTGAT TCTGCCGCCA TGATCCTAGT TAAGAACCCA
  841 ACCCACCTAA ATGGTATTAT AATCACCAGC AACATGTTTG GTGACATCAT CTCCGATGAA
  901 GCCCTCCGTT ATCCCAGGTT TCCTTGGGTT TGTTGCCATC TGCGTCCTTG GCCTCTTTGC
  961 CAGACAAGAA CACCGCATTT GGTTTGTACG AACCCATGCA CGTTCTGCTC CAGATTTGCC
 1021 AAAGATAAGT CCACCCTATC GCACTATCTG GTCTGCCTGC ATGAATGTGA ATGTCATTGA
1081 ACTTGCTGGA GAGGTAAGGC ATTGG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

2018.12.10

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