Sequencing and PCR primers for primer information.
S. pombe expression vector with nmt1 promoter
| Catalog number | RDB06587 |
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| Resource name | pFFH41 |
| Clone info. | S. pombe expression vector with nmt1 promoter |
| Vector backbone | pREP41 (Plasmid) |
| Selectable markers | Amp^r |
| Depositor | Yoshida, Minoru | Matsuyama, Akihisa | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation. |
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| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB06587 | pFFH41 | DNA solution |
Electronic file
| Electronic file | Sequence RDB06587z.seq from Depositor |
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Featured content
| Featured content | Expression Vector Backbone of Schizosaccharomyces pombe (English text) |
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References
| original | Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. |
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User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB14498_A6L7p1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: nmt1Fw | RDB14498_A6L7a.seq |
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>06587_14498_A6L7_nmt1Fw_A10_01_ABI24.ab1 1 TGGAATTTAA TTAAATCAGG AAAACGTAAC TCTCGGCTAC TGGATGGTTC AGTCACCCAA 61 CGATTACTGG GGAGAGAAAA CAGGGCAAAA GCAAAGCTTA AAGGAATCCG ATTGTCATTC 121 GGCAATGTGC AGCGAAACTA AAAACCGGAT AATGGACCTG TTAATCGAAA CATTGAAGAT 181 AAAGGAAGAG GAATCCTGGC ATATCATCAA TTGAATAAGT TGAATTAATT ATTTCAATCT 241 CATTCTCACT TTCTGACTTA TAGTCGCTTT GTTAAAGCTA GCGTCGACAG GCCTGGATCC 301 GGATATCATG GATTATAAGG ATGATGACGA TAAACCCATG GACTACAAGG ACGACGATGA 361 CAAACATCAC CACCATCACC ACTGAGATCT CGAGCCCGGG CGGCCGCTAA CTAACTAAGG 421 GTAAAAGGAA TGTCTCCCTT GCCAGTACTG CTAGGGTTTT TCTTTCAAAC TATGGAAGCC 481 CATTCAAGCT GCATATTACG ATTTTGTTTT TCGCTTTTAG AAAGTGGTTT AGATGAGATA 541 ATAGAAAAAT TCTTGATCTC CGACAACGAG TACTTTTATT TTTTTTGCTA ATCACTTTAC 601 TCAATATTAG CTCGAAATCG TAGAAACGTA GACGGGTGCG GGATACCGAG TGGTGTAGTT 661 AAGAATTTTT ATAAACCACG TGGCCCAAAA ATATGAACCC AAAACGTTTA TACATGAGTA 721 TACTTTAAGA AGGCTATACC CCTTCGTGTT AGATGTAGTT TTAGCTACCC AACCCGAGTC 781 TATGAGCTTG ACTTCAGATG TAGAAGGCAT TAAATCGTTT TGAATATTAA TTAAAAAACG 841 ATGAAAATTA AATATTTAAA AGCAATCATA CGCTGAAAAT TTAGTGCTGT GGCTAATCCT 901 TCAACATGGA AATGCCATAA AAGTGACTTT GACAAAAAAA AAAAAGTATA TACAGGGTAG 961 TAAACTCATC TACTTCATTT GACTTTGTTT ACAGCATGTG GAAGGAAGAA TATTTATTTG 1021 CTAAATTCGT TAGTTTTACC ATTTCCAATA AGTAATACCT AATGAAATCC GACAGATTGG 1081 CGCATGATGA AAAGAGCCAT TTTGCTTTTG GGGAAGAAAC TG // |
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| Primer: f1ori_R3 | RDB14498_A6L7b.seq |
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>06587_14498_A6L7_f1ori_R3_C10_07_ABI24.ab1 1 GCCAGAGGGG CGTTGAGTAT GCGGGTGTGA ATACCGCACA GATGCGTAAG GAGAAAATAC 61 CGCATCAGGC GCCATTCGCC ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCGG 121 GCCTCTTCGC TATTACGCCA GCTGGCGAAA GGGGGATGTG CTGCAAGGCG ATTAAGTTGG 181 GTAACGCCAG GGTTTTCCCA GTCACGACGT TGTAAAACGA CGGCCAGTGA ATTCCAACCT 241 TCCAATTCAT TAAATCGAGT CCTCCATCAG ATGATTTACA AGGTTTTGCA TAGAATCCCT 301 AAGTAAGAGA AGTTGTTAGT GATTTCTTCA AGTTTACTAA AATTTACATA CAAATGGATG 361 ATCTCTTCTC CATTGTTTCC TTTCTTCTTG CAAACGCGTT TTACAAAGAG ATGACATGCT 421 ATTTTCTGAT TATTTTTTTC TATAGTTTTC TATTTTGTAC GTCCTTGGGA GTCCGAAATG 481 TAAAATCGGG GTATGGTTAG TAGCGGCAGC GTTCATTATG GTATATAAAA GATAAAAAGT 541 AGGGGCAAGC GAGACAGAAT GGGATACAAG GGCATCGTCT ATAATTATAG CTAAAAATTG 601 TATTTTAATT TGTATTTTTT GTAATTTTAT TTCTCATTGT TTTACTTAAA ATGAATGCGA 661 ATTAGAGAAA ACTTCAACGA AATGTCAAAA TAAGCTCAGC AAAATATACA ATTTTAGGGA 721 AAGCGATCAG CAACTTATTC TCCGTTGTAG ATTCTTTTTC TTCATAAATT CCAACAATGT 781 AACTGCCAAT TGATTTTTCT CTAGATGCTA ATAAATTGAA ACTAATGAAT ATAATAACAC 841 AGGTTTTGAT AAATTGTAAA CTCTTACTCA AAAAAAAGAA TGTTAAAAAC TAATTTAACC 901 TTTTTATAAA GGCTCATGGG CGTTTAAAGA GGATAGTAAA AATTCTACAA TTTTTTAATT 961 TAAATGTCAC AATTGTCTTG CGAATATAAT TCTAATTAAT TTTTCGCTTA ACTGCTTGTT 1021 TAGTACTATA CTTTCAATAT GCGTTATATG CAACCTTTAT CCATGGAGAT TGCGATATTT 1081 TTGGCAATCT ATCCTGGTAC CTTGGGAGGC GGCGTTCTAA CTCAAC // |
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| Primer: LEU2pro_F | RDB14498_A6L7c.seq |
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>06587_14498_A6L7_LEU2pro_F_B10_04_ABI24.ab1 1 AATCCGGCAG TTGAATATTA TTTAAGGACC TATTGTTTTT TCCAATAGGT GGTTAGCAAT 61 CGTCTTACTT TCTAACTTTT CTTACCTTTT ACATTTCAGC AATATATATA TATATTTCAA 121 GGATATACCA TTCTAATGTC TGCCCCTAAG AAGATCGTCG TTTTGCCAGG TGACCACGTT 181 GGTCAAGAAA TCACAGCCGA AGCCATTAAG GTTCTTAAAG CTATTTCTGA TGTTCGTTCC 241 AATGTCAAGT TCGATTTCGA AAATCATTTA ATTGGTGGTG CTGCTATCGA TGCTACAGGT 301 GTTCCACTTC CAGATGAGGC GCTGGAAGCC TCCAAGAAGG CTGATGCCGT TTTGTTAGGT 361 GCTGTGGGTG GTCCTAAATG GGGTACCGGT AGTGTTAGAC CTGAACAAGG TTTACTAAAA 421 ATCCGTAAAG AACTTCAATT GTACGCCAAC TTAAGACCAT GTAACTTTGC ATCCGACTCT 481 CTTTTAGACT TATCTCCAAT CAAGCCACAA TTTGCTAAAG GTACTGACTT CGTTGTTGTC 541 AGAGAATTAG TGGGAGGTAT TTACTTTGGT AAGAGAAAGG AAGACGATGG TGATGGTGTC 601 GCTTGGGATA GTGAACAATA CACCGTTCCA GAAGTGCAAA GAATCACAAG AATGGCCGCT 661 TTCATGGCCC TACAACATGA GCCACCATTG CCTATTTGGT CCTTGGATAA AGCTAATGTT 721 TTGGCCTCTT CAAGATTATG GAGAAAAACT GTGGAGGAAA CCATCAAGAA CGAATTCCCT 781 ACATTGAAGG TTCAACATCA ATTGATTGAT TCTGCCGCCA TGATCCTAGT TAAGAACCCA 841 ACCCACCTAA ATGGTATTAT AATCACCAGC AACATGTTTG GTGACATCAT CTCCGATGAA 901 GCCCTCCGTT ATCCCAGGTT TCCTTGGGTT TGTTGCCATC TGCGTCCTTG GCCTCTTTGC 961 CAGACAAGAA CACCGCATTT GGTTTGTACG AACCCATGCA CGTTCTGCTC CAGATTTGCC 1021 AAAGATAAGT CCACCCTATC GCACTATCTG GTCTGCCTGC ATGAATGTGA ATGTCATTGA 1081 ACTTGCTGGA GAGGTAAGGC ATTGG // |
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Please visit Sequencing and PCR primers for primer information.
References
| original | Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. |
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2018.12.10
GNP_filter3_RDBDEP_html_181012.pl