Please review the QC test results indicated by icon below as well as clone information before placing your order.

pFFH41

S. pombe expression vector with nmt1 promoter

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.

Ordering

Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、　寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14498_A6L7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw Sequence file: RDB14498_A6L7a.seq
>06587_14498_A6L7_nmt1Fw_A10_01_ABI24.ab1     1 TGGAATTTAA TTAAATCAGG AAAACGTAAC TCTCGGCTAC TGGATGGTTC AGTCACCCAA    61 CGATTACTGG GGAGAGAAAA CAGGGCAAAA GCAAAGCTTA AAGGAATCCG ATTGTCATTC   121 GGCAATGTGC AGCGAAACTA AAAACCGGAT AATGGACCTG TTAATCGAAA CATTGAAGAT   181 AAAGGAAGAG GAATCCTGGC ATATCATCAA TTGAATAAGT TGAATTAATT ATTTCAATCT   241 CATTCTCACT TTCTGACTTA TAGTCGCTTT GTTAAAGCTA GCGTCGACAG GCCTGGATCC   301 GGATATCATG GATTATAAGG ATGATGACGA TAAACCCATG GACTACAAGG ACGACGATGA   361 CAAACATCAC CACCATCACC ACTGAGATCT CGAGCCCGGG CGGCCGCTAA CTAACTAAGG   421 GTAAAAGGAA TGTCTCCCTT GCCAGTACTG CTAGGGTTTT TCTTTCAAAC TATGGAAGCC   481 CATTCAAGCT GCATATTACG ATTTTGTTTT TCGCTTTTAG AAAGTGGTTT AGATGAGATA   541 ATAGAAAAAT TCTTGATCTC CGACAACGAG TACTTTTATT TTTTTTGCTA ATCACTTTAC   601 TCAATATTAG CTCGAAATCG TAGAAACGTA GACGGGTGCG GGATACCGAG TGGTGTAGTT   661 AAGAATTTTT ATAAACCACG TGGCCCAAAA ATATGAACCC AAAACGTTTA TACATGAGTA   721 TACTTTAAGA AGGCTATACC CCTTCGTGTT AGATGTAGTT TTAGCTACCC AACCCGAGTC   781 TATGAGCTTG ACTTCAGATG TAGAAGGCAT TAAATCGTTT TGAATATTAA TTAAAAAACG   841 ATGAAAATTA AATATTTAAA AGCAATCATA CGCTGAAAAT TTAGTGCTGT GGCTAATCCT   901 TCAACATGGA AATGCCATAA AAGTGACTTT GACAAAAAAA AAAAAGTATA TACAGGGTAG   961 TAAACTCATC TACTTCATTT GACTTTGTTT ACAGCATGTG GAAGGAAGAA TATTTATTTG  1021 CTAAATTCGT TAGTTTTACC ATTTCCAATA AGTAATACCT AATGAAATCC GACAGATTGG 1081 CGCATGATGA AAAGAGCCAT TTTGCTTTTG GGGAAGAAAC TG //
Primer: f1ori_R3 Sequence file: RDB14498_A6L7b.seq
>06587_14498_A6L7_f1ori_R3_C10_07_ABI24.ab1     1 GCCAGAGGGG CGTTGAGTAT GCGGGTGTGA ATACCGCACA GATGCGTAAG GAGAAAATAC    61 CGCATCAGGC GCCATTCGCC ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCGG   121 GCCTCTTCGC TATTACGCCA GCTGGCGAAA GGGGGATGTG CTGCAAGGCG ATTAAGTTGG   181 GTAACGCCAG GGTTTTCCCA GTCACGACGT TGTAAAACGA CGGCCAGTGA ATTCCAACCT   241 TCCAATTCAT TAAATCGAGT CCTCCATCAG ATGATTTACA AGGTTTTGCA TAGAATCCCT   301 AAGTAAGAGA AGTTGTTAGT GATTTCTTCA AGTTTACTAA AATTTACATA CAAATGGATG   361 ATCTCTTCTC CATTGTTTCC TTTCTTCTTG CAAACGCGTT TTACAAAGAG ATGACATGCT   421 ATTTTCTGAT TATTTTTTTC TATAGTTTTC TATTTTGTAC GTCCTTGGGA GTCCGAAATG   481 TAAAATCGGG GTATGGTTAG TAGCGGCAGC GTTCATTATG GTATATAAAA GATAAAAAGT   541 AGGGGCAAGC GAGACAGAAT GGGATACAAG GGCATCGTCT ATAATTATAG CTAAAAATTG   601 TATTTTAATT TGTATTTTTT GTAATTTTAT TTCTCATTGT TTTACTTAAA ATGAATGCGA   661 ATTAGAGAAA ACTTCAACGA AATGTCAAAA TAAGCTCAGC AAAATATACA ATTTTAGGGA   721 AAGCGATCAG CAACTTATTC TCCGTTGTAG ATTCTTTTTC TTCATAAATT CCAACAATGT   781 AACTGCCAAT TGATTTTTCT CTAGATGCTA ATAAATTGAA ACTAATGAAT ATAATAACAC   841 AGGTTTTGAT AAATTGTAAA CTCTTACTCA AAAAAAAGAA TGTTAAAAAC TAATTTAACC   901 TTTTTATAAA GGCTCATGGG CGTTTAAAGA GGATAGTAAA AATTCTACAA TTTTTTAATT   961 TAAATGTCAC AATTGTCTTG CGAATATAAT TCTAATTAAT TTTTCGCTTA ACTGCTTGTT  1021 TAGTACTATA CTTTCAATAT GCGTTATATG CAACCTTTAT CCATGGAGAT TGCGATATTT 1081 TTGGCAATCT ATCCTGGTAC CTTGGGAGGC GGCGTTCTAA CTCAAC //
Primer: LEU2pro_F Sequence file: RDB14498_A6L7c.seq
>06587_14498_A6L7_LEU2pro_F_B10_04_ABI24.ab1     1 AATCCGGCAG TTGAATATTA TTTAAGGACC TATTGTTTTT TCCAATAGGT GGTTAGCAAT    61 CGTCTTACTT TCTAACTTTT CTTACCTTTT ACATTTCAGC AATATATATA TATATTTCAA   121 GGATATACCA TTCTAATGTC TGCCCCTAAG AAGATCGTCG TTTTGCCAGG TGACCACGTT   181 GGTCAAGAAA TCACAGCCGA AGCCATTAAG GTTCTTAAAG CTATTTCTGA TGTTCGTTCC   241 AATGTCAAGT TCGATTTCGA AAATCATTTA ATTGGTGGTG CTGCTATCGA TGCTACAGGT   301 GTTCCACTTC CAGATGAGGC GCTGGAAGCC TCCAAGAAGG CTGATGCCGT TTTGTTAGGT   361 GCTGTGGGTG GTCCTAAATG GGGTACCGGT AGTGTTAGAC CTGAACAAGG TTTACTAAAA   421 ATCCGTAAAG AACTTCAATT GTACGCCAAC TTAAGACCAT GTAACTTTGC ATCCGACTCT   481 CTTTTAGACT TATCTCCAAT CAAGCCACAA TTTGCTAAAG GTACTGACTT CGTTGTTGTC   541 AGAGAATTAG TGGGAGGTAT TTACTTTGGT AAGAGAAAGG AAGACGATGG TGATGGTGTC   601 GCTTGGGATA GTGAACAATA CACCGTTCCA GAAGTGCAAA GAATCACAAG AATGGCCGCT   661 TTCATGGCCC TACAACATGA GCCACCATTG CCTATTTGGT CCTTGGATAA AGCTAATGTT   721 TTGGCCTCTT CAAGATTATG GAGAAAAACT GTGGAGGAAA CCATCAAGAA CGAATTCCCT   781 ACATTGAAGG TTCAACATCA ATTGATTGAT TCTGCCGCCA TGATCCTAGT TAAGAACCCA   841 ACCCACCTAA ATGGTATTAT AATCACCAGC AACATGTTTG GTGACATCAT CTCCGATGAA   901 GCCCTCCGTT ATCCCAGGTT TCCTTGGGTT TGTTGCCATC TGCGTCCTTG GCCTCTTTGC   961 CAGACAAGAA CACCGCATTT GGTTTGTACG AACCCATGCA CGTTCTGCTC CAGATTTGCC  1021 AAAGATAAGT CCACCCTATC GCACTATCTG GTCTGCCTGC ATGAATGTGA ATGTCATTGA 1081 ACTTGCTGGA GAGGTAAGGC ATTGG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.02.09

GNP_filter3_RDBDEP_html_220112.pl