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pHFG1 (#RDB06583)

S. pombe expression vector with nmt1 promoter

Clone info. S. pombe expression vector with nmt1 promoter
Vector backbone pREP1 (Plasmid)
Selectable markers Amp^r
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
 
Sequence RDB06583z.seq

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、　寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06583 pHFG1 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pHFG1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06583).

Reference section:

Matsuyama, A., Shirai, A., Yashiroda, Y., Kamata, A., Horinouchi, S., Yoshida, M., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14472_A6L7p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw
Sequence file: RDB14472_A6L7a.seq check
>06583_14472_A6L7_nmt1Fw_A11_01_ABI08.ab1
    1 AAGAAAAATT AATTAAATCA GGAAAAACGT AACTCTCGGC TACTGGATGG TTCAGTCACC
   61 CAACGATTAC TGGGGAGAGA AAACAGGGCA AAAGCAAAGC TTAAAGGAAT CCGATTGTCA
  121 TTCGGCAATG TGCAGCGAAA CTAAAAACCG GATAATGGAC CTGTTAATCG AAACATTGAA
  181 GATATATAAA GGAAGAGGAA TCCTGGCATA TCATCAATTG AATAAGTTGA ATTAATTATT
  241 TCAATCTCAT TCTCACTTTC TGACTTATAG TCGCTTTGTT AAAGCTAGCG TCGACAGGCC
  301 TGGATCCTAT GCATCACCAC CATCATCATA TGGATTATAA GGATGATGAC GATAAACCCA
  361 TGGGTAAAGG AGAAGAACTT TTCACTGGAG TTGTCCCAAT TCTTGTTGAA TTAGATGGTG
  421 ATGTTAATGG GCACAAATTT TCTGTCAGTG GAGAGGGTGA AGGTGATGCA ACATACGGAA
  481 AACTTACCCT TAAATTTATT TGCACTACTG GAAAACTACC TGTTCCGTGG CCAACACTTG
  541 TCACTACTTT CTGTTATGGT GTTCAATGCT TTTCAAGATA CCCAGATCAC ATGAAACAGC
  601 ATGACTTTTT CAAGAGTGCC ATGCCCGAAG GCTATGTACA GGAAAGAACT ATATTTTTCA
  661 AAGATGACGG GAACTACAAG ACACGTGCTG AAGTCAAGTT TGAAGGTGAT ACCCTTGTTA
  721 ATAGAATCGA GTTAAAAGGT ATTGATTTTA AAGAAGATGG AAACATTCTT GGACACAAAT
  781 TGGAATACAA CTATAACTCA CACAATGTAT ACATCATGGC AGACAAACAA AAGAATGGAA
  841 TCAAAGTTAA CTTCAAAATT AGACACAACA TTGAAGATGG AAGCGTTCAA CTAGCAGACC
  901 ATTATCAACA AAATACTCCA ATTGGCGATG GCCCTGTCCT TTTACCAGAC AACCATTACC
  961 TGTCCACACA ATCTGCCCTT TCGAAAGATC CCAACGAAAA GAGAGACCAC ATGGGTCCTT
 1021 TCTTGAGTTT GTAACAGCTG CTGGGATTAC ACATGGCCAT GGATGAACTA ATACAAGGAT
 1081 ATCATGGAGA TCTCGAGGCC GGGCCGGCGC TACCTACCTT AGGTTAAGGA ATGTCTCTGG
1141 CAGTTACCTG CCTAAGGTTT TCTTTTCGAC ATTATGAGAA GGCCCCATTT CCAG
//
Primer: nmt1ter_R
Sequence file: RDB14472_A6L7b.seq check
>06583_14472_A6L7_nmt1ter_R_B11_02_ABI08.ab1
    1 CAACCTTCTA GTTACTCATG TATAAACGTT TTGGGTTCAT ATTTTTGGGC CACGTGGTTT
   61 ATAAAAATTC TTAACTACAC CACTCGGTAT CCCGCACCCG TCTACGTTTC TACGATTTCG
  121 AGCTAATATT GAGTAAAGTG ATTAGCAAAA AAAATAAAAG TACTCGTTGT CGGAGATCAA
  181 GAATTTTTCT ATTATCTCAT CTAAACCACT TTCTAAAAGC GAAAAACAAA ATCGTAATAT
  241 GCAGCTTGAA TGGGCTTCCA TAGTTTGAAA GAAAAACCCT AGCAGTACTG GCAAGGGAGA
  301 CATTCCTTTT ACCCTTAGTT AGTTAGCGGC CGCCCGGGCT CGAGATCTCA TGATATCTTT
  361 GTATAGTTCA TCCATGCCAT GTGTAATCCC AGCAGCTGTT ACAAACTCAA GAAGGACCAT
  421 GTGGTCTCTC TTTTCGTTGG GATCTTTCGA AAGGGCAGAT TGTGTGGACA GGTAATGGTT
  481 GTCTGGTAAA AGGACAGGGC CATCGCCAAT TGGAGTATTT TGTTGATAAT GGTCTGCTAG
  541 TTGAACGCTT CCATCTTCAA TGTTGTGTCT AATTTTGAAG TTAACTTTGA TTCCATTCTT
  601 TTGTTTGTCT GCCATGATGT ATACATTGTG TGAGTTATAG TTGTATTCCA ATTTGTGTCC
  661 AAGAATGTTT CCATCTTCTT TAAAATCAAT ACCTTTTAAC TCGATTCTAT TAACAAGGGT
  721 ATCACCTTCA AACTTGACTT CAGCACGTGT CTTGTAGTTC CCGTCATCTT TGAAAAATAT
  781 AGTTCTTTCC TGTACATAGC CTTCGGGCAT GGCACTCTTG AAAAAGTCAT GCTGTTTCAT
  841 GTGATCTGGG TATCTTGAAA AGCATTGAAC ACCATAACAG AAAGTAGTGA CAAGTGTTGG
  901 CCACGGAACA CGTAGTTTTC CAGTAGTGCA AATAAATTTA AAGGGTAAGT TTTCCGTATG
  961 TTGCATCACC TTCACCCTCT CCACTGACAG AAAATTTGTG CCCATTAACA TCACCATCTA
 1021 ATTCAACAAG AATTGGGACA ACTCCCAGTG AAAAGTCTTC TCTTACCCAT GGGGTTTATC
 1081 GTCATCAATC TATTATTCCA TATGATGGAT TGTGGTGATG CCATAGGATT CAGGCTGGTC
1141 GAACGCTAGC CTTAACAAAG CGGACCTTAT TAAGATTCCA GA
//
Primer: f1ori_R3
Sequence file: RDB14472_A6L7c.seq check
>06583_14472_A6L7_f1ori_R3_C11_03_ABI08.ab1
    1 GCCAGGGGGG GCTTGGAGTA TGCGGTGTGA ATACCGCACA GATGCGTAAG GAGAAAATAC
   61 CGCATCAGGC GCCATTCGCC ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCGG
  121 GCCTCTTCGC TATTACGCCA GCTGGCGAAA GGGGGATGTG CTGCAAGGCG ATTAAGTTGG
  181 GTAACGCCAG GGTTTTCCCA GTCACGACGT TGTAAAACGA CGGCCAGTGA ATTCCAACCT
  241 TCCAATTCAT TAAATCGAGT CCTCCATCAG ATGATTTACA AGGTTTTGCA TAGAATCCCT
  301 AAGTAAGAGA AGTTGTTAGT GATTTCTTCA AGTTTACTAA AATTTACATA CAAATGGATG
  361 ATCTCTTCTC CATTGTTTCC TTTCTTCTTG CAAACGCGTT TTACAAAGAG ATGACATGCT
  421 ATTTTCTGAT TATTTTTTTC TATAGTTTTC TATTTTGTAC GTCCTTGGGA GTCCGAAATG
  481 TAAAATCGGG GTATGGTTAG TAGCGGCAGC GTTCATTATG GTATATAAAA GATAAAAAGT
  541 AGGGGCAAGC GAGACAGAAT GGGATACAAG GGCATCGTCT ATAATTATAG CTAAAAATTG
  601 TATTTTAATT TGTATTTTTT GTAATTTTAT TTCTCATTGT TTTACTTAAA ATGAATGCGA
  661 ATTAGAGAAA ACTTCAACGA AATGTCAAAA TAAGCTCAGC AAAATATACA ATTTTAGGGA
  721 AAGCGATCAG CAACTTATTC TCCGTTGTAG ATTCTTTTTC TTCATAAATT CCAACAATGT
  781 AACTGCCAAT TGATTTTTCT CTAGATGCTA ATAAATTGAA ACTAATGAAT ATAATAACAC
  841 AGGTTTTGAT AAATTGTAAA CTCTTACTCA AAAAAAGAAT GTTAAAACTA ATTTAACCTT
  901 TTTATAAAGG CTCATGGCGT TAAAGAGGAT AGTAAAAATT CTACAATTTT TTTAATTTAA
  961 TGTCACAATT GTCTTGCGAA TATAATTCTA TTAATTTTCG CTAACTGCTG TTTTAGTACT
 1021 ATTACTTTCA ATATGCCCTT ATATGCAACT TTAATTCATG AAGAAATGCA ATATTTTTGC
 1081 AATCTTCTTG TACTGAGCGC TTTTTTATCA TTTAGTACTC ATTATTTAAG TTTTCAGTTA
1141 AGCCATTAAT TAATTTGTAG AGATGCAATT GTAAATCCCT CCCTTCTATA TTCCCGAG
//
Primer: LEU2pro_F
Sequence file: RDB14472_A6L7d.seq check
>06583_14472_A6L7_LEU2pro_F_D11_04_ABI08.ab1
    1 CATCATTGAG TTGAATATTA TTTAAGGACC TATTGTTTTT TCCAATAGGT GGTTAGCAAT
   61 CGTCTTACTT TCTAACTTTT CTTACCTTTT ACATTTCAGC AATATATATA TATATTTCAA
  121 GGATATACCA TTCTAATGTC TGCCCCTAAG AAGATCGTCG TTTTGCCAGG TGACCACGTT
  181 GGTCAAGAAA TCACAGCCGA AGCCATTAAG GTTCTTAAAG CTATTTCTGA TGTTCGTTCC
  241 AATGTCAAGT TCGATTTCGA AAATCATTTA ATTGGTGGTG CTGCTATCGA TGCTACAGGT
  301 GTTCCACTTC CAGATGAGGC GCTGGAAGCC TCCAAGAAGG CTGATGCCGT TTTGTTAGGT
  361 GCTGTGGGTG GTCCTAAATG GGGTACCGGT AGTGTTAGAC CTGAACAAGG TTTACTAAAA
  421 ATCCGTAAAG AACTTCAATT GTACGCCAAC TTAAGACCAT GTAACTTTGC ATCCGACTCT
  481 CTTTTAGACT TATCTCCAAT CAAGCCACAA TTTGCTAAAG GTACTGACTT CGTTGTTGTC
  541 AGAGAATTAG TGGGAGGTAT TTACTTTGGT AAGAGAAAGG AAGACGATGG TGATGGTGTC
  601 GCTTGGGATA GTGAACAATA CACCGTTCCA GAAGTGCAAA GAATCACAAG AATGGCCGCT
  661 TTCATGGCCC TACAACATGA GCCACCATTG CCTATTTGGT CCTTGGATAA AGCTAATGTT
  721 TTGGCCTCTT CAAGATTATG GAGAAAAACT GTGGAGGAAA CCATCAAGAA CGAATTCCCT
  781 ACATTGAAGG TTCAACATCA ATTGATTGAT TCTGCCGCCA TGATCCTAGT TAAGAACCCA
  841 ACCCACCTAA ATGGTATTAT AATCACCAGC AACATGTTTG GTGACATCAT CTCCGATGAA
  901 GCCTCCGTTA TCCCAGGTTC CTTGGGTTTG TTGCCATCTG CGTCCTTGGC CTCTTTGCCA
  961 GACAAGAACA CCGCATTTGG TTTGTACGAA CCATGCCACG GTTCTGCTCC AGATTTGCCA
 1021 AAGAATAAGT CAACCCTATC GCCACTATCT TGTCTGCTGC ATGATGTGAA ATGTCATTGA
 1081 ACTGCCCTGA GAGTAGCCAT TGAGATGCAG TTAAAGGTTG GAATGCAAGG TATCAGAACC
1141 TGTTGAATTA AGGGTGGGCT TCCAAACAGT ACCACGCG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. [link to RRC of NBRP]