Sequencing and PCR primers for primer information.
S. pombe expression vector with nmt1 promoter
| Catalog number | RDB06583 |
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| Resource name | pHFG1 |
| Clone info. | S. pombe expression vector with nmt1 promoter |
| Vector backbone | pREP1 (Plasmid) |
| Selectable markers | Amp^r |
| Depositor | Yoshida, Minoru | Matsuyama, Akihisa | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation. |
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| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Schedule A (GFP-A) [Link] |
| Remarks | ((Additional form)) GFP-A Please use Schedule A (GFP-A) additionally. |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB06583 | pHFG1 | DNA solution |
Electronic file
| Electronic file | Sequence RDB06583z.seq from Depositor |
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Featured content
| Featured content | Expression Vector Backbone of Schizosaccharomyces pombe (English text) |
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References
| original | Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. |
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User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB14472_A6L7p1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: nmt1Fw | RDB14472_A6L7a.seq |
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>06583_14472_A6L7_nmt1Fw_A11_01_ABI08.ab1 1 AAGAAAAATT AATTAAATCA GGAAAAACGT AACTCTCGGC TACTGGATGG TTCAGTCACC 61 CAACGATTAC TGGGGAGAGA AAACAGGGCA AAAGCAAAGC TTAAAGGAAT CCGATTGTCA 121 TTCGGCAATG TGCAGCGAAA CTAAAAACCG GATAATGGAC CTGTTAATCG AAACATTGAA 181 GATATATAAA GGAAGAGGAA TCCTGGCATA TCATCAATTG AATAAGTTGA ATTAATTATT 241 TCAATCTCAT TCTCACTTTC TGACTTATAG TCGCTTTGTT AAAGCTAGCG TCGACAGGCC 301 TGGATCCTAT GCATCACCAC CATCATCATA TGGATTATAA GGATGATGAC GATAAACCCA 361 TGGGTAAAGG AGAAGAACTT TTCACTGGAG TTGTCCCAAT TCTTGTTGAA TTAGATGGTG 421 ATGTTAATGG GCACAAATTT TCTGTCAGTG GAGAGGGTGA AGGTGATGCA ACATACGGAA 481 AACTTACCCT TAAATTTATT TGCACTACTG GAAAACTACC TGTTCCGTGG CCAACACTTG 541 TCACTACTTT CTGTTATGGT GTTCAATGCT TTTCAAGATA CCCAGATCAC ATGAAACAGC 601 ATGACTTTTT CAAGAGTGCC ATGCCCGAAG GCTATGTACA GGAAAGAACT ATATTTTTCA 661 AAGATGACGG GAACTACAAG ACACGTGCTG AAGTCAAGTT TGAAGGTGAT ACCCTTGTTA 721 ATAGAATCGA GTTAAAAGGT ATTGATTTTA AAGAAGATGG AAACATTCTT GGACACAAAT 781 TGGAATACAA CTATAACTCA CACAATGTAT ACATCATGGC AGACAAACAA AAGAATGGAA 841 TCAAAGTTAA CTTCAAAATT AGACACAACA TTGAAGATGG AAGCGTTCAA CTAGCAGACC 901 ATTATCAACA AAATACTCCA ATTGGCGATG GCCCTGTCCT TTTACCAGAC AACCATTACC 961 TGTCCACACA ATCTGCCCTT TCGAAAGATC CCAACGAAAA GAGAGACCAC ATGGGTCCTT 1021 TCTTGAGTTT GTAACAGCTG CTGGGATTAC ACATGGCCAT GGATGAACTA ATACAAGGAT 1081 ATCATGGAGA TCTCGAGGCC GGGCCGGCGC TACCTACCTT AGGTTAAGGA ATGTCTCTGG 1141 CAGTTACCTG CCTAAGGTTT TCTTTTCGAC ATTATGAGAA GGCCCCATTT CCAG // |
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| Primer: nmt1ter_R | RDB14472_A6L7b.seq |
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>06583_14472_A6L7_nmt1ter_R_B11_02_ABI08.ab1 1 CAACCTTCTA GTTACTCATG TATAAACGTT TTGGGTTCAT ATTTTTGGGC CACGTGGTTT 61 ATAAAAATTC TTAACTACAC CACTCGGTAT CCCGCACCCG TCTACGTTTC TACGATTTCG 121 AGCTAATATT GAGTAAAGTG ATTAGCAAAA AAAATAAAAG TACTCGTTGT CGGAGATCAA 181 GAATTTTTCT ATTATCTCAT CTAAACCACT TTCTAAAAGC GAAAAACAAA ATCGTAATAT 241 GCAGCTTGAA TGGGCTTCCA TAGTTTGAAA GAAAAACCCT AGCAGTACTG GCAAGGGAGA 301 CATTCCTTTT ACCCTTAGTT AGTTAGCGGC CGCCCGGGCT CGAGATCTCA TGATATCTTT 361 GTATAGTTCA TCCATGCCAT GTGTAATCCC AGCAGCTGTT ACAAACTCAA GAAGGACCAT 421 GTGGTCTCTC TTTTCGTTGG GATCTTTCGA AAGGGCAGAT TGTGTGGACA GGTAATGGTT 481 GTCTGGTAAA AGGACAGGGC CATCGCCAAT TGGAGTATTT TGTTGATAAT GGTCTGCTAG 541 TTGAACGCTT CCATCTTCAA TGTTGTGTCT AATTTTGAAG TTAACTTTGA TTCCATTCTT 601 TTGTTTGTCT GCCATGATGT ATACATTGTG TGAGTTATAG TTGTATTCCA ATTTGTGTCC 661 AAGAATGTTT CCATCTTCTT TAAAATCAAT ACCTTTTAAC TCGATTCTAT TAACAAGGGT 721 ATCACCTTCA AACTTGACTT CAGCACGTGT CTTGTAGTTC CCGTCATCTT TGAAAAATAT 781 AGTTCTTTCC TGTACATAGC CTTCGGGCAT GGCACTCTTG AAAAAGTCAT GCTGTTTCAT 841 GTGATCTGGG TATCTTGAAA AGCATTGAAC ACCATAACAG AAAGTAGTGA CAAGTGTTGG 901 CCACGGAACA CGTAGTTTTC CAGTAGTGCA AATAAATTTA AAGGGTAAGT TTTCCGTATG 961 TTGCATCACC TTCACCCTCT CCACTGACAG AAAATTTGTG CCCATTAACA TCACCATCTA 1021 ATTCAACAAG AATTGGGACA ACTCCCAGTG AAAAGTCTTC TCTTACCCAT GGGGTTTATC 1081 GTCATCAATC TATTATTCCA TATGATGGAT TGTGGTGATG CCATAGGATT CAGGCTGGTC 1141 GAACGCTAGC CTTAACAAAG CGGACCTTAT TAAGATTCCA GA // |
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| Primer: f1ori_R3 | RDB14472_A6L7c.seq |
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>06583_14472_A6L7_f1ori_R3_C11_03_ABI08.ab1 1 GCCAGGGGGG GCTTGGAGTA TGCGGTGTGA ATACCGCACA GATGCGTAAG GAGAAAATAC 61 CGCATCAGGC GCCATTCGCC ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCGG 121 GCCTCTTCGC TATTACGCCA GCTGGCGAAA GGGGGATGTG CTGCAAGGCG ATTAAGTTGG 181 GTAACGCCAG GGTTTTCCCA GTCACGACGT TGTAAAACGA CGGCCAGTGA ATTCCAACCT 241 TCCAATTCAT TAAATCGAGT CCTCCATCAG ATGATTTACA AGGTTTTGCA TAGAATCCCT 301 AAGTAAGAGA AGTTGTTAGT GATTTCTTCA AGTTTACTAA AATTTACATA CAAATGGATG 361 ATCTCTTCTC CATTGTTTCC TTTCTTCTTG CAAACGCGTT TTACAAAGAG ATGACATGCT 421 ATTTTCTGAT TATTTTTTTC TATAGTTTTC TATTTTGTAC GTCCTTGGGA GTCCGAAATG 481 TAAAATCGGG GTATGGTTAG TAGCGGCAGC GTTCATTATG GTATATAAAA GATAAAAAGT 541 AGGGGCAAGC GAGACAGAAT GGGATACAAG GGCATCGTCT ATAATTATAG CTAAAAATTG 601 TATTTTAATT TGTATTTTTT GTAATTTTAT TTCTCATTGT TTTACTTAAA ATGAATGCGA 661 ATTAGAGAAA ACTTCAACGA AATGTCAAAA TAAGCTCAGC AAAATATACA ATTTTAGGGA 721 AAGCGATCAG CAACTTATTC TCCGTTGTAG ATTCTTTTTC TTCATAAATT CCAACAATGT 781 AACTGCCAAT TGATTTTTCT CTAGATGCTA ATAAATTGAA ACTAATGAAT ATAATAACAC 841 AGGTTTTGAT AAATTGTAAA CTCTTACTCA AAAAAAGAAT GTTAAAACTA ATTTAACCTT 901 TTTATAAAGG CTCATGGCGT TAAAGAGGAT AGTAAAAATT CTACAATTTT TTTAATTTAA 961 TGTCACAATT GTCTTGCGAA TATAATTCTA TTAATTTTCG CTAACTGCTG TTTTAGTACT 1021 ATTACTTTCA ATATGCCCTT ATATGCAACT TTAATTCATG AAGAAATGCA ATATTTTTGC 1081 AATCTTCTTG TACTGAGCGC TTTTTTATCA TTTAGTACTC ATTATTTAAG TTTTCAGTTA 1141 AGCCATTAAT TAATTTGTAG AGATGCAATT GTAAATCCCT CCCTTCTATA TTCCCGAG // |
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| Primer: LEU2pro_F | RDB14472_A6L7d.seq |
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>06583_14472_A6L7_LEU2pro_F_D11_04_ABI08.ab1 1 CATCATTGAG TTGAATATTA TTTAAGGACC TATTGTTTTT TCCAATAGGT GGTTAGCAAT 61 CGTCTTACTT TCTAACTTTT CTTACCTTTT ACATTTCAGC AATATATATA TATATTTCAA 121 GGATATACCA TTCTAATGTC TGCCCCTAAG AAGATCGTCG TTTTGCCAGG TGACCACGTT 181 GGTCAAGAAA TCACAGCCGA AGCCATTAAG GTTCTTAAAG CTATTTCTGA TGTTCGTTCC 241 AATGTCAAGT TCGATTTCGA AAATCATTTA ATTGGTGGTG CTGCTATCGA TGCTACAGGT 301 GTTCCACTTC CAGATGAGGC GCTGGAAGCC TCCAAGAAGG CTGATGCCGT TTTGTTAGGT 361 GCTGTGGGTG GTCCTAAATG GGGTACCGGT AGTGTTAGAC CTGAACAAGG TTTACTAAAA 421 ATCCGTAAAG AACTTCAATT GTACGCCAAC TTAAGACCAT GTAACTTTGC ATCCGACTCT 481 CTTTTAGACT TATCTCCAAT CAAGCCACAA TTTGCTAAAG GTACTGACTT CGTTGTTGTC 541 AGAGAATTAG TGGGAGGTAT TTACTTTGGT AAGAGAAAGG AAGACGATGG TGATGGTGTC 601 GCTTGGGATA GTGAACAATA CACCGTTCCA GAAGTGCAAA GAATCACAAG AATGGCCGCT 661 TTCATGGCCC TACAACATGA GCCACCATTG CCTATTTGGT CCTTGGATAA AGCTAATGTT 721 TTGGCCTCTT CAAGATTATG GAGAAAAACT GTGGAGGAAA CCATCAAGAA CGAATTCCCT 781 ACATTGAAGG TTCAACATCA ATTGATTGAT TCTGCCGCCA TGATCCTAGT TAAGAACCCA 841 ACCCACCTAA ATGGTATTAT AATCACCAGC AACATGTTTG GTGACATCAT CTCCGATGAA 901 GCCTCCGTTA TCCCAGGTTC CTTGGGTTTG TTGCCATCTG CGTCCTTGGC CTCTTTGCCA 961 GACAAGAACA CCGCATTTGG TTTGTACGAA CCATGCCACG GTTCTGCTCC AGATTTGCCA 1021 AAGAATAAGT CAACCCTATC GCCACTATCT TGTCTGCTGC ATGATGTGAA ATGTCATTGA 1081 ACTGCCCTGA GAGTAGCCAT TGAGATGCAG TTAAAGGTTG GAATGCAAGG TATCAGAACC 1141 TGTTGAATTA AGGGTGGGCT TCCAAACAGT ACCACGCG // |
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Please visit Sequencing and PCR primers for primer information.
References
| original | Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. |
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2018.12.10
GNP_filter3_RDBDEP_html_181012.pl