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pHFG1

S. pombe expression vector with nmt1 promoter

Catalog number RDB06583
Resource name pHFG1
Clone info. S. pombe expression vector with nmt1 promoter
Vector backbone pREP1 (Plasmid)
Selectable markers Amp^r
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
Other clones in our bank

External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
            Ordering Please visit Information of Request for Distribution.[link] 
            Order form 
            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Schedule A (GFP-A) [Link]
            Remarks
            Please use Schedule A (GFP-A) additionally.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、 寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            Schedule A(GFP-A, 英語版のみ) [Link]
            備考
            Schedule A(GFP-A, 英語版のみ)が追加で必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB06583 pHFG1 DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB06583z.seq

            Original reference

            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB14472_A6L7p1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: nmt1Fw
            Sequence file: RDB14472_A6L7a.seq check
            >06583_14472_A6L7_nmt1Fw_A11_01_ABI08.ab1
                1 AAGAAAAATT AATTAAATCA GGAAAAACGT AACTCTCGGC TACTGGATGG TTCAGTCACC
               61 CAACGATTAC TGGGGAGAGA AAACAGGGCA AAAGCAAAGC TTAAAGGAAT CCGATTGTCA
              121 TTCGGCAATG TGCAGCGAAA CTAAAAACCG GATAATGGAC CTGTTAATCG AAACATTGAA
              181 GATATATAAA GGAAGAGGAA TCCTGGCATA TCATCAATTG AATAAGTTGA ATTAATTATT
              241 TCAATCTCAT TCTCACTTTC TGACTTATAG TCGCTTTGTT AAAGCTAGCG TCGACAGGCC
              301 TGGATCCTAT GCATCACCAC CATCATCATA TGGATTATAA GGATGATGAC GATAAACCCA
              361 TGGGTAAAGG AGAAGAACTT TTCACTGGAG TTGTCCCAAT TCTTGTTGAA TTAGATGGTG
              421 ATGTTAATGG GCACAAATTT TCTGTCAGTG GAGAGGGTGA AGGTGATGCA ACATACGGAA
              481 AACTTACCCT TAAATTTATT TGCACTACTG GAAAACTACC TGTTCCGTGG CCAACACTTG
              541 TCACTACTTT CTGTTATGGT GTTCAATGCT TTTCAAGATA CCCAGATCAC ATGAAACAGC
              601 ATGACTTTTT CAAGAGTGCC ATGCCCGAAG GCTATGTACA GGAAAGAACT ATATTTTTCA
              661 AAGATGACGG GAACTACAAG ACACGTGCTG AAGTCAAGTT TGAAGGTGAT ACCCTTGTTA
              721 ATAGAATCGA GTTAAAAGGT ATTGATTTTA AAGAAGATGG AAACATTCTT GGACACAAAT
              781 TGGAATACAA CTATAACTCA CACAATGTAT ACATCATGGC AGACAAACAA AAGAATGGAA
              841 TCAAAGTTAA CTTCAAAATT AGACACAACA TTGAAGATGG AAGCGTTCAA CTAGCAGACC
              901 ATTATCAACA AAATACTCCA ATTGGCGATG GCCCTGTCCT TTTACCAGAC AACCATTACC
              961 TGTCCACACA ATCTGCCCTT TCGAAAGATC CCAACGAAAA GAGAGACCAC ATGGGTCCTT
             1021 TCTTGAGTTT GTAACAGCTG CTGGGATTAC ACATGGCCAT GGATGAACTA ATACAAGGAT
             1081 ATCATGGAGA TCTCGAGGCC GGGCCGGCGC TACCTACCTT AGGTTAAGGA ATGTCTCTGG
            1141 CAGTTACCTG CCTAAGGTTT TCTTTTCGAC ATTATGAGAA GGCCCCATTT CCAG
            //
            Primer: nmt1ter_R
            Sequence file: RDB14472_A6L7b.seq check
            >06583_14472_A6L7_nmt1ter_R_B11_02_ABI08.ab1
                1 CAACCTTCTA GTTACTCATG TATAAACGTT TTGGGTTCAT ATTTTTGGGC CACGTGGTTT
               61 ATAAAAATTC TTAACTACAC CACTCGGTAT CCCGCACCCG TCTACGTTTC TACGATTTCG
              121 AGCTAATATT GAGTAAAGTG ATTAGCAAAA AAAATAAAAG TACTCGTTGT CGGAGATCAA
              181 GAATTTTTCT ATTATCTCAT CTAAACCACT TTCTAAAAGC GAAAAACAAA ATCGTAATAT
              241 GCAGCTTGAA TGGGCTTCCA TAGTTTGAAA GAAAAACCCT AGCAGTACTG GCAAGGGAGA
              301 CATTCCTTTT ACCCTTAGTT AGTTAGCGGC CGCCCGGGCT CGAGATCTCA TGATATCTTT
              361 GTATAGTTCA TCCATGCCAT GTGTAATCCC AGCAGCTGTT ACAAACTCAA GAAGGACCAT
              421 GTGGTCTCTC TTTTCGTTGG GATCTTTCGA AAGGGCAGAT TGTGTGGACA GGTAATGGTT
              481 GTCTGGTAAA AGGACAGGGC CATCGCCAAT TGGAGTATTT TGTTGATAAT GGTCTGCTAG
              541 TTGAACGCTT CCATCTTCAA TGTTGTGTCT AATTTTGAAG TTAACTTTGA TTCCATTCTT
              601 TTGTTTGTCT GCCATGATGT ATACATTGTG TGAGTTATAG TTGTATTCCA ATTTGTGTCC
              661 AAGAATGTTT CCATCTTCTT TAAAATCAAT ACCTTTTAAC TCGATTCTAT TAACAAGGGT
              721 ATCACCTTCA AACTTGACTT CAGCACGTGT CTTGTAGTTC CCGTCATCTT TGAAAAATAT
              781 AGTTCTTTCC TGTACATAGC CTTCGGGCAT GGCACTCTTG AAAAAGTCAT GCTGTTTCAT
              841 GTGATCTGGG TATCTTGAAA AGCATTGAAC ACCATAACAG AAAGTAGTGA CAAGTGTTGG
              901 CCACGGAACA CGTAGTTTTC CAGTAGTGCA AATAAATTTA AAGGGTAAGT TTTCCGTATG
              961 TTGCATCACC TTCACCCTCT CCACTGACAG AAAATTTGTG CCCATTAACA TCACCATCTA
             1021 ATTCAACAAG AATTGGGACA ACTCCCAGTG AAAAGTCTTC TCTTACCCAT GGGGTTTATC
             1081 GTCATCAATC TATTATTCCA TATGATGGAT TGTGGTGATG CCATAGGATT CAGGCTGGTC
            1141 GAACGCTAGC CTTAACAAAG CGGACCTTAT TAAGATTCCA GA
            //
            Primer: f1ori_R3
            Sequence file: RDB14472_A6L7c.seq check
            >06583_14472_A6L7_f1ori_R3_C11_03_ABI08.ab1
                1 GCCAGGGGGG GCTTGGAGTA TGCGGTGTGA ATACCGCACA GATGCGTAAG GAGAAAATAC
               61 CGCATCAGGC GCCATTCGCC ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCGG
              121 GCCTCTTCGC TATTACGCCA GCTGGCGAAA GGGGGATGTG CTGCAAGGCG ATTAAGTTGG
              181 GTAACGCCAG GGTTTTCCCA GTCACGACGT TGTAAAACGA CGGCCAGTGA ATTCCAACCT
              241 TCCAATTCAT TAAATCGAGT CCTCCATCAG ATGATTTACA AGGTTTTGCA TAGAATCCCT
              301 AAGTAAGAGA AGTTGTTAGT GATTTCTTCA AGTTTACTAA AATTTACATA CAAATGGATG
              361 ATCTCTTCTC CATTGTTTCC TTTCTTCTTG CAAACGCGTT TTACAAAGAG ATGACATGCT
              421 ATTTTCTGAT TATTTTTTTC TATAGTTTTC TATTTTGTAC GTCCTTGGGA GTCCGAAATG
              481 TAAAATCGGG GTATGGTTAG TAGCGGCAGC GTTCATTATG GTATATAAAA GATAAAAAGT
              541 AGGGGCAAGC GAGACAGAAT GGGATACAAG GGCATCGTCT ATAATTATAG CTAAAAATTG
              601 TATTTTAATT TGTATTTTTT GTAATTTTAT TTCTCATTGT TTTACTTAAA ATGAATGCGA
              661 ATTAGAGAAA ACTTCAACGA AATGTCAAAA TAAGCTCAGC AAAATATACA ATTTTAGGGA
              721 AAGCGATCAG CAACTTATTC TCCGTTGTAG ATTCTTTTTC TTCATAAATT CCAACAATGT
              781 AACTGCCAAT TGATTTTTCT CTAGATGCTA ATAAATTGAA ACTAATGAAT ATAATAACAC
              841 AGGTTTTGAT AAATTGTAAA CTCTTACTCA AAAAAAGAAT GTTAAAACTA ATTTAACCTT
              901 TTTATAAAGG CTCATGGCGT TAAAGAGGAT AGTAAAAATT CTACAATTTT TTTAATTTAA
              961 TGTCACAATT GTCTTGCGAA TATAATTCTA TTAATTTTCG CTAACTGCTG TTTTAGTACT
             1021 ATTACTTTCA ATATGCCCTT ATATGCAACT TTAATTCATG AAGAAATGCA ATATTTTTGC
             1081 AATCTTCTTG TACTGAGCGC TTTTTTATCA TTTAGTACTC ATTATTTAAG TTTTCAGTTA
            1141 AGCCATTAAT TAATTTGTAG AGATGCAATT GTAAATCCCT CCCTTCTATA TTCCCGAG
            //
            Primer: LEU2pro_F
            Sequence file: RDB14472_A6L7d.seq check
            >06583_14472_A6L7_LEU2pro_F_D11_04_ABI08.ab1
                1 CATCATTGAG TTGAATATTA TTTAAGGACC TATTGTTTTT TCCAATAGGT GGTTAGCAAT
               61 CGTCTTACTT TCTAACTTTT CTTACCTTTT ACATTTCAGC AATATATATA TATATTTCAA
              121 GGATATACCA TTCTAATGTC TGCCCCTAAG AAGATCGTCG TTTTGCCAGG TGACCACGTT
              181 GGTCAAGAAA TCACAGCCGA AGCCATTAAG GTTCTTAAAG CTATTTCTGA TGTTCGTTCC
              241 AATGTCAAGT TCGATTTCGA AAATCATTTA ATTGGTGGTG CTGCTATCGA TGCTACAGGT
              301 GTTCCACTTC CAGATGAGGC GCTGGAAGCC TCCAAGAAGG CTGATGCCGT TTTGTTAGGT
              361 GCTGTGGGTG GTCCTAAATG GGGTACCGGT AGTGTTAGAC CTGAACAAGG TTTACTAAAA
              421 ATCCGTAAAG AACTTCAATT GTACGCCAAC TTAAGACCAT GTAACTTTGC ATCCGACTCT
              481 CTTTTAGACT TATCTCCAAT CAAGCCACAA TTTGCTAAAG GTACTGACTT CGTTGTTGTC
              541 AGAGAATTAG TGGGAGGTAT TTACTTTGGT AAGAGAAAGG AAGACGATGG TGATGGTGTC
              601 GCTTGGGATA GTGAACAATA CACCGTTCCA GAAGTGCAAA GAATCACAAG AATGGCCGCT
              661 TTCATGGCCC TACAACATGA GCCACCATTG CCTATTTGGT CCTTGGATAA AGCTAATGTT
              721 TTGGCCTCTT CAAGATTATG GAGAAAAACT GTGGAGGAAA CCATCAAGAA CGAATTCCCT
              781 ACATTGAAGG TTCAACATCA ATTGATTGAT TCTGCCGCCA TGATCCTAGT TAAGAACCCA
              841 ACCCACCTAA ATGGTATTAT AATCACCAGC AACATGTTTG GTGACATCAT CTCCGATGAA
              901 GCCTCCGTTA TCCCAGGTTC CTTGGGTTTG TTGCCATCTG CGTCCTTGGC CTCTTTGCCA
              961 GACAAGAACA CCGCATTTGG TTTGTACGAA CCATGCCACG GTTCTGCTCC AGATTTGCCA
             1021 AAGAATAAGT CAACCCTATC GCCACTATCT TGTCTGCTGC ATGATGTGAA ATGTCATTGA
             1081 ACTGCCCTGA GAGTAGCCAT TGAGATGCAG TTAAAGGTTG GAATGCAAGG TATCAGAACC
            1141 TGTTGAATTA AGGGTGGGCT TCCAAACAGT ACCACGCG
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

            2022.02.09

            GNP_filter3_RDBDEP_html_220112.pl