Please review the QC test results indicated by icon below as well as clone information before placing your order.

pHFG1

S. pombe expression vector with nmt1 promoter

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Schedule A (GFP-A) [Link]
Remarks	Please use Schedule A (GFP-A) additionally.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、　寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] Schedule A（GFP-A, 英語版のみ） [Link]
備考	Schedule A（GFP-A, 英語版のみ）が追加で必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14472_A6L7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw Sequence file: RDB14472_A6L7a.seq
>06583_14472_A6L7_nmt1Fw_A11_01_ABI08.ab1     1 AAGAAAAATT AATTAAATCA GGAAAAACGT AACTCTCGGC TACTGGATGG TTCAGTCACC    61 CAACGATTAC TGGGGAGAGA AAACAGGGCA AAAGCAAAGC TTAAAGGAAT CCGATTGTCA   121 TTCGGCAATG TGCAGCGAAA CTAAAAACCG GATAATGGAC CTGTTAATCG AAACATTGAA   181 GATATATAAA GGAAGAGGAA TCCTGGCATA TCATCAATTG AATAAGTTGA ATTAATTATT   241 TCAATCTCAT TCTCACTTTC TGACTTATAG TCGCTTTGTT AAAGCTAGCG TCGACAGGCC   301 TGGATCCTAT GCATCACCAC CATCATCATA TGGATTATAA GGATGATGAC GATAAACCCA   361 TGGGTAAAGG AGAAGAACTT TTCACTGGAG TTGTCCCAAT TCTTGTTGAA TTAGATGGTG   421 ATGTTAATGG GCACAAATTT TCTGTCAGTG GAGAGGGTGA AGGTGATGCA ACATACGGAA   481 AACTTACCCT TAAATTTATT TGCACTACTG GAAAACTACC TGTTCCGTGG CCAACACTTG   541 TCACTACTTT CTGTTATGGT GTTCAATGCT TTTCAAGATA CCCAGATCAC ATGAAACAGC   601 ATGACTTTTT CAAGAGTGCC ATGCCCGAAG GCTATGTACA GGAAAGAACT ATATTTTTCA   661 AAGATGACGG GAACTACAAG ACACGTGCTG AAGTCAAGTT TGAAGGTGAT ACCCTTGTTA   721 ATAGAATCGA GTTAAAAGGT ATTGATTTTA AAGAAGATGG AAACATTCTT GGACACAAAT   781 TGGAATACAA CTATAACTCA CACAATGTAT ACATCATGGC AGACAAACAA AAGAATGGAA   841 TCAAAGTTAA CTTCAAAATT AGACACAACA TTGAAGATGG AAGCGTTCAA CTAGCAGACC   901 ATTATCAACA AAATACTCCA ATTGGCGATG GCCCTGTCCT TTTACCAGAC AACCATTACC   961 TGTCCACACA ATCTGCCCTT TCGAAAGATC CCAACGAAAA GAGAGACCAC ATGGGTCCTT  1021 TCTTGAGTTT GTAACAGCTG CTGGGATTAC ACATGGCCAT GGATGAACTA ATACAAGGAT  1081 ATCATGGAGA TCTCGAGGCC GGGCCGGCGC TACCTACCTT AGGTTAAGGA ATGTCTCTGG 1141 CAGTTACCTG CCTAAGGTTT TCTTTTCGAC ATTATGAGAA GGCCCCATTT CCAG //
Primer: nmt1ter_R Sequence file: RDB14472_A6L7b.seq
>06583_14472_A6L7_nmt1ter_R_B11_02_ABI08.ab1     1 CAACCTTCTA GTTACTCATG TATAAACGTT TTGGGTTCAT ATTTTTGGGC CACGTGGTTT    61 ATAAAAATTC TTAACTACAC CACTCGGTAT CCCGCACCCG TCTACGTTTC TACGATTTCG   121 AGCTAATATT GAGTAAAGTG ATTAGCAAAA AAAATAAAAG TACTCGTTGT CGGAGATCAA   181 GAATTTTTCT ATTATCTCAT CTAAACCACT TTCTAAAAGC GAAAAACAAA ATCGTAATAT   241 GCAGCTTGAA TGGGCTTCCA TAGTTTGAAA GAAAAACCCT AGCAGTACTG GCAAGGGAGA   301 CATTCCTTTT ACCCTTAGTT AGTTAGCGGC CGCCCGGGCT CGAGATCTCA TGATATCTTT   361 GTATAGTTCA TCCATGCCAT GTGTAATCCC AGCAGCTGTT ACAAACTCAA GAAGGACCAT   421 GTGGTCTCTC TTTTCGTTGG GATCTTTCGA AAGGGCAGAT TGTGTGGACA GGTAATGGTT   481 GTCTGGTAAA AGGACAGGGC CATCGCCAAT TGGAGTATTT TGTTGATAAT GGTCTGCTAG   541 TTGAACGCTT CCATCTTCAA TGTTGTGTCT AATTTTGAAG TTAACTTTGA TTCCATTCTT   601 TTGTTTGTCT GCCATGATGT ATACATTGTG TGAGTTATAG TTGTATTCCA ATTTGTGTCC   661 AAGAATGTTT CCATCTTCTT TAAAATCAAT ACCTTTTAAC TCGATTCTAT TAACAAGGGT   721 ATCACCTTCA AACTTGACTT CAGCACGTGT CTTGTAGTTC CCGTCATCTT TGAAAAATAT   781 AGTTCTTTCC TGTACATAGC CTTCGGGCAT GGCACTCTTG AAAAAGTCAT GCTGTTTCAT   841 GTGATCTGGG TATCTTGAAA AGCATTGAAC ACCATAACAG AAAGTAGTGA CAAGTGTTGG   901 CCACGGAACA CGTAGTTTTC CAGTAGTGCA AATAAATTTA AAGGGTAAGT TTTCCGTATG   961 TTGCATCACC TTCACCCTCT CCACTGACAG AAAATTTGTG CCCATTAACA TCACCATCTA  1021 ATTCAACAAG AATTGGGACA ACTCCCAGTG AAAAGTCTTC TCTTACCCAT GGGGTTTATC  1081 GTCATCAATC TATTATTCCA TATGATGGAT TGTGGTGATG CCATAGGATT CAGGCTGGTC 1141 GAACGCTAGC CTTAACAAAG CGGACCTTAT TAAGATTCCA GA //
Primer: f1ori_R3 Sequence file: RDB14472_A6L7c.seq
>06583_14472_A6L7_f1ori_R3_C11_03_ABI08.ab1     1 GCCAGGGGGG GCTTGGAGTA TGCGGTGTGA ATACCGCACA GATGCGTAAG GAGAAAATAC    61 CGCATCAGGC GCCATTCGCC ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCGG   121 GCCTCTTCGC TATTACGCCA GCTGGCGAAA GGGGGATGTG CTGCAAGGCG ATTAAGTTGG   181 GTAACGCCAG GGTTTTCCCA GTCACGACGT TGTAAAACGA CGGCCAGTGA ATTCCAACCT   241 TCCAATTCAT TAAATCGAGT CCTCCATCAG ATGATTTACA AGGTTTTGCA TAGAATCCCT   301 AAGTAAGAGA AGTTGTTAGT GATTTCTTCA AGTTTACTAA AATTTACATA CAAATGGATG   361 ATCTCTTCTC CATTGTTTCC TTTCTTCTTG CAAACGCGTT TTACAAAGAG ATGACATGCT   421 ATTTTCTGAT TATTTTTTTC TATAGTTTTC TATTTTGTAC GTCCTTGGGA GTCCGAAATG   481 TAAAATCGGG GTATGGTTAG TAGCGGCAGC GTTCATTATG GTATATAAAA GATAAAAAGT   541 AGGGGCAAGC GAGACAGAAT GGGATACAAG GGCATCGTCT ATAATTATAG CTAAAAATTG   601 TATTTTAATT TGTATTTTTT GTAATTTTAT TTCTCATTGT TTTACTTAAA ATGAATGCGA   661 ATTAGAGAAA ACTTCAACGA AATGTCAAAA TAAGCTCAGC AAAATATACA ATTTTAGGGA   721 AAGCGATCAG CAACTTATTC TCCGTTGTAG ATTCTTTTTC TTCATAAATT CCAACAATGT   781 AACTGCCAAT TGATTTTTCT CTAGATGCTA ATAAATTGAA ACTAATGAAT ATAATAACAC   841 AGGTTTTGAT AAATTGTAAA CTCTTACTCA AAAAAAGAAT GTTAAAACTA ATTTAACCTT   901 TTTATAAAGG CTCATGGCGT TAAAGAGGAT AGTAAAAATT CTACAATTTT TTTAATTTAA   961 TGTCACAATT GTCTTGCGAA TATAATTCTA TTAATTTTCG CTAACTGCTG TTTTAGTACT  1021 ATTACTTTCA ATATGCCCTT ATATGCAACT TTAATTCATG AAGAAATGCA ATATTTTTGC  1081 AATCTTCTTG TACTGAGCGC TTTTTTATCA TTTAGTACTC ATTATTTAAG TTTTCAGTTA 1141 AGCCATTAAT TAATTTGTAG AGATGCAATT GTAAATCCCT CCCTTCTATA TTCCCGAG //
Primer: LEU2pro_F Sequence file: RDB14472_A6L7d.seq
>06583_14472_A6L7_LEU2pro_F_D11_04_ABI08.ab1     1 CATCATTGAG TTGAATATTA TTTAAGGACC TATTGTTTTT TCCAATAGGT GGTTAGCAAT    61 CGTCTTACTT TCTAACTTTT CTTACCTTTT ACATTTCAGC AATATATATA TATATTTCAA   121 GGATATACCA TTCTAATGTC TGCCCCTAAG AAGATCGTCG TTTTGCCAGG TGACCACGTT   181 GGTCAAGAAA TCACAGCCGA AGCCATTAAG GTTCTTAAAG CTATTTCTGA TGTTCGTTCC   241 AATGTCAAGT TCGATTTCGA AAATCATTTA ATTGGTGGTG CTGCTATCGA TGCTACAGGT   301 GTTCCACTTC CAGATGAGGC GCTGGAAGCC TCCAAGAAGG CTGATGCCGT TTTGTTAGGT   361 GCTGTGGGTG GTCCTAAATG GGGTACCGGT AGTGTTAGAC CTGAACAAGG TTTACTAAAA   421 ATCCGTAAAG AACTTCAATT GTACGCCAAC TTAAGACCAT GTAACTTTGC ATCCGACTCT   481 CTTTTAGACT TATCTCCAAT CAAGCCACAA TTTGCTAAAG GTACTGACTT CGTTGTTGTC   541 AGAGAATTAG TGGGAGGTAT TTACTTTGGT AAGAGAAAGG AAGACGATGG TGATGGTGTC   601 GCTTGGGATA GTGAACAATA CACCGTTCCA GAAGTGCAAA GAATCACAAG AATGGCCGCT   661 TTCATGGCCC TACAACATGA GCCACCATTG CCTATTTGGT CCTTGGATAA AGCTAATGTT   721 TTGGCCTCTT CAAGATTATG GAGAAAAACT GTGGAGGAAA CCATCAAGAA CGAATTCCCT   781 ACATTGAAGG TTCAACATCA ATTGATTGAT TCTGCCGCCA TGATCCTAGT TAAGAACCCA   841 ACCCACCTAA ATGGTATTAT AATCACCAGC AACATGTTTG GTGACATCAT CTCCGATGAA   901 GCCTCCGTTA TCCCAGGTTC CTTGGGTTTG TTGCCATCTG CGTCCTTGGC CTCTTTGCCA   961 GACAAGAACA CCGCATTTGG TTTGTACGAA CCATGCCACG GTTCTGCTCC AGATTTGCCA  1021 AAGAATAAGT CAACCCTATC GCCACTATCT TGTCTGCTGC ATGATGTGAA ATGTCATTGA  1081 ACTGCCCTGA GAGTAGCCAT TGAGATGCAG TTAAAGGTTG GAATGCAAGG TATCAGAACC 1141 TGTTGAATTA AGGGTGGGCT TCCAAACAGT ACCACGCG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.02.09

GNP_filter3_RDBDEP_html_220112.pl