Resource data sheet
Prev.

pLYS1U-Pnmt1

S. pombe expression vector with nmt1 promoter

Catalog number RDB06567
Resource name pLYS1U-Pnmt1
Clone info. S. pombe expression vector with nmt1 promoter
Vector backbone pUC119 (Plasmid)
Selectable markers Amp^r
Depositor Yoshida, Minoru | Matsuyama, Akihisa |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Methods Mol. Biol. 824: 433-450 (2012)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
Ordering Please visit Information of Request for Distribution. 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06567 pLYS1U-Pnmt1 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB06567z.seq from Depositor

Featured content

Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

References

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB06567_A4Kj.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 RDB06567_A4Kja.seq
>06567_06567_A4Kj_M13(-40)_A07_01.ab1
    1 GCGGGCAGGG ATTCGAGCTC GTTTAACGTG ATTTTTATCT TGTTTGTCTA CATGGTATTT
   61 TACATTCATC TACATACATC TTTCATTGGC TTTGTACATA GTTATCATTA CAAGTCTAAA
  121 AAAATTCACT CTTTTCTTAT TCAATGTCAA TCCAAGAGAA AAGATTGTGG TAATGTTGTA
  181 GGAGCATGTT TAATAAATTA CTATAGCAAA TTACTTTTTA TTCCCAAGGT GTTTATCTAT
  241 AATAGTTAAT ATTTTAGTCG CTACATAAAA TTTTACCAAA GAGTACTTGT ATACTAATTC
  301 TAAATGCCTT CTGACATAAA ACGCCTAGGA AAACAAACGC AAACAAGGCA TCGACTTTTT
  361 CAATAACCAA CCAAAAAAAT TTTACATTAG TCTTTTTTTA ATGCTGAGAA AGTCTTTGCT
  421 GATATGCCTT CCAACCAGCT TCTCTATATC TCTTGGCTTC GACAACAGGA TTACGACCAG
  481 CTCCATAGAC TCCACGACCA ACAATGATGA TATCGCTACC GCAGTTTACA ATCACTTCTT
  541 CAGGAGTACG ATATTGCTGT CCCAGCCCGT CTCCTTTAAC ATCCAAGCCG ATACCAGGGG
  601 ACATAGTTAT GTAGTCGCTT TGAAGGTTAG GAAATCGACG ACCAGCTATA AAGCCAAAGC
  661 AAAAATCGGT ATGCTTCTCA AACCATTCTA AGGTTTTCTC TGTGTAGGAA CCAGTAGCCA
  721 AAGAGCCTTT GGAAGACATT TCAGCCAAAA GCAAGAGACC ACGTCCCAAA GGTAAACCAA
  781 CTTCTTTGAG GCCTTGTATA ATACCCTCGC CTGGCACTGT ATGGCAATTT GTGATATGAG
  841 CCCAAGAAGC AATTTTGTAC ACACCAGATG CATATTGTAG CTTGACGGTA TTTCCAATGT
  901 CTGCGAATTT GCGATCCTCA AAGATAAGAA AACGATGCTT TTTACCTAAA GGCCACCAGT
  961 TTTTCTACCA TATCCTGGTC GAAATCCTCG ACAACGTCAA TATGTGTCTT GATAACACAG
 1021 ACATAGGGTC CAATTTTATC TACCAAATTC TAAGATTTCG GATTTCTTCG TCAATCGACC
 1081 GCGACTGACA GTGCTTTGCT TTTTCTTCAT CAAGCCAACA ATCTGCCATT GGAATTTTTC
1141 ATCCCCCTCA GCTTCTAGCC TGGAAATATA GAGCT
//
Primer: nmt1Fw RDB06567_A4Kjb.seq
>06567_06567_A4Kj_nmt1Fw_B07_04.ab1
    1 GGGATTTAAT TAATCAGGGA AAACGTAACT CTCGGCTACT GGATGGTTCA GTCACCCAAC
   61 GATTACTGGG GAGAGAAAAC AGGGCAAAAG CAAAGCTTAA AGGAATCCGA TTGTCATTCG
  121 GCAATGTGCA GCGAAACTAA AAACCGGATA ATGGACCTGT TAATCGAAAC ATTGAAGATA
  181 TATAAAGGAA GAGGAATCCT GGCATATCAT CAATTGAATA AGTTGAATTA ATTATTTCAA
  241 TCTCATTCTC ACTTTCTGAC TTATAGTCGC TTTGTTAAAG CTAGCGTCGA CAGGCCTGGA
  301 TCCGGCGCGC CACTTCTAAA TAAGCGAATT TCTTATGATT TATGATTTTT ATTATTAAAT
  361 AAGTTATAAA AAAAATAAGT GTATACAAAT TTTAAAGTGA CTCTTAGGTT TTAAAACGAA
  421 AATTCTTATT CTTGAGTAAC TCTTTCCTGT AGGTCAGGTT GCTTTCTCAG GTATAGTATG
  481 AGGTCGCTCT TATTGACCAC ACCTCTACCG GCAGATCCGC TAGGGATAAC AGGGTAATAT
  541 ACTAGTACTT TGTAGTACCT GCGCGGTCTC AAGATCAAAC ATTTTTGGAA TCGCAAAAAG
  601 ATGTCATTCC AGCCGGACGT GGCATGAAGA ATGTCCAACT TTTGGTGATC AATCGTTTTG
  661 ATACTAACAA GATATGCGGT ATCGGAGAAG TTGGTGAAAT ATATCTTCGT GCTGGTGGAT
  721 TGGCTGAGGG CTACCTTGGA AATGATGAAT TGACTAGCAA AAAATTCTTA AAGAGTTGGT
  781 TTGCTGATCC CTCCAAGTTT GTAGACCGAA CTCCAGAAAA TGCGCCCTGG AAACCTTATT
  841 GGTTCGGAAT ACGCGATCGT ATGTATCGTT CTGGTGACTT GGGCCGCTAT CTCCCAACTG
  901 GTAATGTAGA AATGTAGCGG CCGCGGGCCC GCGGCCGCCG ACGATCAAAT TAAAATTCGT
  961 GGTTTCCGAA TTGAGTTAGG TGAAATTAAT ACCCATCTTT CTCGTCATCC TAATGTCCGT
 1021 GAAAATATTA CCCTTTGTTC GACGTGATAA GGATGAGAAC CCACGCTAGT TGCATACATT
 1081 GTACCCTCAA GTTAATAAAG ATGACTTTGA TAGCGCTTAC TGAGTCCGAG GATATTGGTT
1141 CGTTCATGGA TTGAGAGTAA CAAGGAAGCT TTTTATT
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.

2018.12.10

GNP_filter3_RDBDEP_html_181012.pl