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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pLYS1U-Pnmt1

S. pombe expression vector with nmt1 promoter

Catalog number RDB06567
Resource name pLYS1U-Pnmt1
Clone info. S. pombe expression vector with nmt1 promoter
Vector backbone pUC119 (Plasmid)
Selectable markers Amp^r
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Methods Mol. Biol. 824: 433-450 (2012)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
            Ordering Please visit Information of Request for Distribution.[link] 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Methods Mol. Biol. 824: 433-450 (2012)) の引用と、寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB06567 pLYS1U-Pnmt1 DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB06567z.seq

            Original reference

            original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB06567_A4Kj.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: M13_-40
            Sequence file: RDB06567_A4Kja.seq check
            >06567_06567_A4Kj_M13(-40)_A07_01.ab1
                1 GCGGGCAGGG ATTCGAGCTC GTTTAACGTG ATTTTTATCT TGTTTGTCTA CATGGTATTT
               61 TACATTCATC TACATACATC TTTCATTGGC TTTGTACATA GTTATCATTA CAAGTCTAAA
              121 AAAATTCACT CTTTTCTTAT TCAATGTCAA TCCAAGAGAA AAGATTGTGG TAATGTTGTA
              181 GGAGCATGTT TAATAAATTA CTATAGCAAA TTACTTTTTA TTCCCAAGGT GTTTATCTAT
              241 AATAGTTAAT ATTTTAGTCG CTACATAAAA TTTTACCAAA GAGTACTTGT ATACTAATTC
              301 TAAATGCCTT CTGACATAAA ACGCCTAGGA AAACAAACGC AAACAAGGCA TCGACTTTTT
              361 CAATAACCAA CCAAAAAAAT TTTACATTAG TCTTTTTTTA ATGCTGAGAA AGTCTTTGCT
              421 GATATGCCTT CCAACCAGCT TCTCTATATC TCTTGGCTTC GACAACAGGA TTACGACCAG
              481 CTCCATAGAC TCCACGACCA ACAATGATGA TATCGCTACC GCAGTTTACA ATCACTTCTT
              541 CAGGAGTACG ATATTGCTGT CCCAGCCCGT CTCCTTTAAC ATCCAAGCCG ATACCAGGGG
              601 ACATAGTTAT GTAGTCGCTT TGAAGGTTAG GAAATCGACG ACCAGCTATA AAGCCAAAGC
              661 AAAAATCGGT ATGCTTCTCA AACCATTCTA AGGTTTTCTC TGTGTAGGAA CCAGTAGCCA
              721 AAGAGCCTTT GGAAGACATT TCAGCCAAAA GCAAGAGACC ACGTCCCAAA GGTAAACCAA
              781 CTTCTTTGAG GCCTTGTATA ATACCCTCGC CTGGCACTGT ATGGCAATTT GTGATATGAG
              841 CCCAAGAAGC AATTTTGTAC ACACCAGATG CATATTGTAG CTTGACGGTA TTTCCAATGT
              901 CTGCGAATTT GCGATCCTCA AAGATAAGAA AACGATGCTT TTTACCTAAA GGCCACCAGT
              961 TTTTCTACCA TATCCTGGTC GAAATCCTCG ACAACGTCAA TATGTGTCTT GATAACACAG
             1021 ACATAGGGTC CAATTTTATC TACCAAATTC TAAGATTTCG GATTTCTTCG TCAATCGACC
             1081 GCGACTGACA GTGCTTTGCT TTTTCTTCAT CAAGCCAACA ATCTGCCATT GGAATTTTTC
            1141 ATCCCCCTCA GCTTCTAGCC TGGAAATATA GAGCT
            //
            Primer: nmt1Fw
            Sequence file: RDB06567_A4Kjb.seq check
            >06567_06567_A4Kj_nmt1Fw_B07_04.ab1
                1 GGGATTTAAT TAATCAGGGA AAACGTAACT CTCGGCTACT GGATGGTTCA GTCACCCAAC
               61 GATTACTGGG GAGAGAAAAC AGGGCAAAAG CAAAGCTTAA AGGAATCCGA TTGTCATTCG
              121 GCAATGTGCA GCGAAACTAA AAACCGGATA ATGGACCTGT TAATCGAAAC ATTGAAGATA
              181 TATAAAGGAA GAGGAATCCT GGCATATCAT CAATTGAATA AGTTGAATTA ATTATTTCAA
              241 TCTCATTCTC ACTTTCTGAC TTATAGTCGC TTTGTTAAAG CTAGCGTCGA CAGGCCTGGA
              301 TCCGGCGCGC CACTTCTAAA TAAGCGAATT TCTTATGATT TATGATTTTT ATTATTAAAT
              361 AAGTTATAAA AAAAATAAGT GTATACAAAT TTTAAAGTGA CTCTTAGGTT TTAAAACGAA
              421 AATTCTTATT CTTGAGTAAC TCTTTCCTGT AGGTCAGGTT GCTTTCTCAG GTATAGTATG
              481 AGGTCGCTCT TATTGACCAC ACCTCTACCG GCAGATCCGC TAGGGATAAC AGGGTAATAT
              541 ACTAGTACTT TGTAGTACCT GCGCGGTCTC AAGATCAAAC ATTTTTGGAA TCGCAAAAAG
              601 ATGTCATTCC AGCCGGACGT GGCATGAAGA ATGTCCAACT TTTGGTGATC AATCGTTTTG
              661 ATACTAACAA GATATGCGGT ATCGGAGAAG TTGGTGAAAT ATATCTTCGT GCTGGTGGAT
              721 TGGCTGAGGG CTACCTTGGA AATGATGAAT TGACTAGCAA AAAATTCTTA AAGAGTTGGT
              781 TTGCTGATCC CTCCAAGTTT GTAGACCGAA CTCCAGAAAA TGCGCCCTGG AAACCTTATT
              841 GGTTCGGAAT ACGCGATCGT ATGTATCGTT CTGGTGACTT GGGCCGCTAT CTCCCAACTG
              901 GTAATGTAGA AATGTAGCGG CCGCGGGCCC GCGGCCGCCG ACGATCAAAT TAAAATTCGT
              961 GGTTTCCGAA TTGAGTTAGG TGAAATTAAT ACCCATCTTT CTCGTCATCC TAATGTCCGT
             1021 GAAAATATTA CCCTTTGTTC GACGTGATAA GGATGAGAAC CCACGCTAGT TGCATACATT
             1081 GTACCCTCAA GTTAATAAAG ATGACTTTGA TAGCGCTTAC TGAGTCCGAG GATATTGGTT
            1141 CGTTCATGGA TTGAGAGTAA CAAGGAAGCT TTTTATT
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.

            2022.02.09

            GNP_filter3_RDBDEP_html_220112.pl