RDB06520 - pREP41-ccdB2

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pREP41-ccdB2 (#RDB06520)

S. pombe vector with nmt1 (medium strength) promoter

Clone info.	S. pombe vector with nmt1 (medium strength) promoter.
Comment	Gateway compatible.
Vector backbone	pREP41 (Plasmid)
Size of vector backbone	10.5 kb
Selectable markers	E. coli: Amp^r, Cm^r, ccdB.
Growth remarks	Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor\|Developer	Yoshida, Minoru \| Matsuyama, Akihisa \|

Sequence	RDB06520z.seq

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature(s). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.

Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature(s). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
Remarks	Use Survival2 (Invitrogen) or equivalent to amplify this clone.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献の引用と、寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
備考	このクローンの増殖にはSurvival2 (Invitrogen) あるいは同等の宿主を使用してください。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB06520	pREP41-ccdB2	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pREP41-ccdB2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06520).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB12284_A5L2p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw Sequence file: RDB12284_A5L2a.seq
>06520_12284_A5L2_nmt1Fw_E12_15.ab1 1 GAAAATTTTA TTAAATCAGG GGAAAACGTA ACTCTCGGCT ACTGGATGGT TCAGTCACCC 61 AACGATTACT GGGGAGAGAA AACAGGGCAA AAGCAAAGCT TAAAGGAATC CGATTGTCAT 121 TCGGCAATGT GCAGCGAAAC TAAAAACCGG ATAATGGACC TGTTAATCGA AACATTGAAG 181 ATAAAGGAAG AGGAATCCTG GCATATCATC AATTGAATAA GTTGAATTAA TTATTTCAAT 241 CTCATTCTCA CTTTCTGACT TATAGTCGCT TTGTTAAAGC TAGCGTCGAC AGGCCTGGAT 301 CCGGATATCA ACAAGTTTGT ACAAAAAAGC TGAACGAGAA ACGTAAAATG ATATAAATAT 361 CAATATATTA AATTAGATTT TGCATAAAAA ACAGACTACA TAATACTGTA AAACACAACA 421 TATCCAGTCA CTATGGCGGC CGCATTAGGC ACCCCAGGCT TTACACTTTA TGCTTCCGGC 481 TCGTATAATG TGTGGATTTT GAGTTAGGAT CCGTCGAGAT TTTCAGGAGC TAAGGAAGCT 541 AAAATGGAGA AAAAAATCAC TGGATATACC ACCGTTGATA TATCCCAATG GCATCGTAAA 601 GAACATTTTG AGGCATTTCA GTCAGTTGCT CAATGTACCT ATAACCAGAC CGTTCAGCTG 661 GATATTACGG CCTTTTTAAA GACCGTAAAG AAAAATAAGC ACAAGTTTTA TCCGGCCTTT 721 ATTCACATTC TTGCCCGCCT GATGAATGCT CATCCGGAAT TCCGTATGGC AATGAAAGAC 781 GGTGAGCTGG TGATATGGGA TAGTGTTCAC CCTTGTTACA CCGTTTTCCA TGAGCAAACT 841 GAAACGTTTT CATCGCTCTG GAGTGAATAC CACGACGATT TCCGGCAGTT TCTACACATA 901 TATTCGCAAG ATGTGGCGTG TTACGGTGAA AACCTGGCCC TATTTCCCTA AAGGGTTTAT 961 TGAGAATATG TTTTTCGTCT CAGCCAATCC CTGGGTGAGT TTCACAGTTT TGATTTAACG 1021 TATCATATGG GACACTTTCT TCGCCCCCGT TTTCAACAAT GGGCAATATT ATACGCGGCC 1081 GACCAGTTGC TGATGGCGGC TGCGATTCAG TTCATCATGC CGATGTGAAT GCCATGCAAT 1141 GTTCGGCAGA ATGGCCTA //
Primer: nmt1Rv Sequence file: RDB12284_A5L2b.seq
>06520_12284_A5L2_nmt1Rv_F12_18.ab1 1 AGCGGCGGGG GCTCGAGATC TCATGATATC ACCACTTTGT ACAAGAAAGC TGAACGAGAA 61 ACGTAAAATG ATATAAATAT CAATATATTA AATTAGATTT TGCATAAAAA ACAGACTACA 121 TAATACTGTA AAACACAACA TATCCAGTCA CTATGGTCGA CCTGCAGACT GGCTGTGTAT 181 AAGGGAGCCT GACATTTATA TTCCCCAGAA CATCAGGTTA ATGGCGTTTT TGATGTCATT 241 TTCGCGGTGG CTGAGATCAG CCACTTCTTC CCCGATAACG GAGACCGGCA CACTGGCCAT 301 ATCGGTGGTC ATCATGCGCC AGCTTTCATC CCCGATATGC ACCACCGGGT AAAGTTCACG 361 GGAGACTTTA TCTGACAGCA GACGTGCACT GGCCAGGGGG ATCACCATCC GTCGCCCGGG 421 CGTGTCAATA ATATCACTCT GTACATCCAC AAACAGACGA TAACGGCTCT CTCTTTTATA 481 GGTGTAAACC TTAAACTGCA TTTCACCAGC CCCTGTTCTC GTCAGCAAAA GAGCCGTTCA 541 TTTCAATAAA CCGGGCGACC TCAGCCATCC CTTCCTGATT TTCCGCTTTC CAGCGTTCGG 601 CACGCAGACG ACGGGCTTCA TTCTGCATGG TTGTGCTTAC CAGACCGGAG ATATTGACAT 661 CATATATGCC TTGAGCAACT GATAGCTGTC GCTGTCAACT GTCACTGTAA TACGCTGCTT 721 CATAGCATAC CTCTTTTTGA CATACTTCGG GTATACATAT CAGTATATAT TCTTATACCG 781 CAAAAATCAG CGCGCAAATA CGCATACTGT TATCTGGCTT TTAGTAAGCC GGATCCACGC 841 GTTTACGCCC CGCCCTGCCA CTCATCGCAG TACTGTTGTA ATTCATTAAG CATTCTGCCG 901 ACATGGAAGC CATCACAAAC GGCATGATGA ACCTGAATCG CCAGCGGCAT CAGCACCTTG 961 TCGCCTTGCG TATAATATTT GCCCATGGGT GAAAACGGGG GGCGAAGAAG TTGTCCATAT 1021 TGGCCACGTT TAAATCAAAA CTGGTGAAAC TCACCCAGGG ATTGGCTGAG ACGAAAAACA 1081 TATCTCAATA AAACCTTTAG GAATCGTAGC TTTTCACGTA CACGGCACAT CCTGCATCTA 1141 TTGTGTAGCA AGCCTTCCCA GA //
Primer: LEU2pro_F Sequence file: RDB12284_A5L2c.seq
>06520_12284_A5L2_LEU2pro_F_G12_21.ab1 1 ACTGCAGTGA ATATTATTTA AGGACCTATT GTTTTTTCCA ATAGGTGGTT AGCAATCGTC 61 TTACTTTCTA ACTTTTCTTA CCTTTTACAT TTCAGCAATA TATATATATA TTTCAAGGAT 121 ATACCATTCT AATGTCTGCC CCTAAGAAGA TCGTCGTTTT GCCAGGTGAC CACGTTGGTC 181 AAGAAATCAC AGCCGAAGCC ATTAAGGTTC TTAAAGCTAT TTCTGATGTT CGTTCCAATG 241 TCAAGTTCGA TTTCGAAAAT CATTTAATTG GTGGTGCTGC TATCGATGCT ACAGGTGTTC 301 CACTTCCAGA TGAGGCGCTG GAAGCCTCCA AGAAGGCTGA TGCCGTTTTG TTAGGTGCTG 361 TGGGTGGTCC TAAATGGGGT ACCGGTAGTG TTAGACCTGA ACAAGGTTTA CTAAAAATCC 421 GTAAAGAACT TCAATTGTAC GCCAACTTAA GACCATGTAA CTTTGCATCC GACTCTCTTT 481 TAGACTTATC TCCAATCAAG CCACAATTTG CTAAAGGTAC TGACTTCGTT GTTGTCAGAG 541 AATTAGTGGG AGGTATTTAC TTTGGTAAGA GAAAGGAAGA CGATGGTGAT GGTGTCGCTT 601 GGGATAGTGA ACAATACACC GTTCCAGAAG TGCAAAGAAT CACAAGAATG GCCGCTTTCA 661 TGGCCCTACA ACATGAGCCA CCATTGCCTA TTTGGTCCTT GGATAAAGCT AATGTTTTGG 721 CCTCTTCAAG ATTATGGAGA AAAACTGTGG AGGAAACCAT CAAGAACGAA TTCCCTACAT 781 TGAAGGTTCA ACATCAATTG ATTGATTCTG CCGCCATGAT CCTAGTTAAG AACCCAACCC 841 ACCTAAATGG TATTATAATC ACCAGCAACA TGTTTGGTGA CATCATCTCC GATGAAGCCT 901 CCGTTATCCC AGGTTTCCTT GGGTTTGTTG CCATCTGCGT CTTGGCCTCT TTGCCAGACA 961 GACACCGCAT TGGTTTGTAC GACATGCCAC GGTCTGCTCA GATTGCTAAG AATAGTCACC 1021 TATCGCCACT ATCTTGTCTG CTGCATGATG GTGAATGTCA ATGGAACCTG GCTGGACACG 1081 TAGCCATGAA TGCAGTTAAA CGGTTGGCAA TGCAGGAAAT CGA //
Primer: f1ori_R3 Sequence file: RDB12284_A5L2d.seq
>06520_12284_A5L2_f1ori_R3_H12_24.ab1 1 GGTGGGATCT TGAGTATGCG GTGTGAATAC CGCACAGATG CGTAAGGAGA AAATACCGCA 61 TCAGGCGCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT 121 CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA 181 CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGAATTC CAACCTTCCA 241 ATTCATTAAA TCGAGTCCTC CATCAGATGA TTTACAAGGT TTTGCATAGA ATCCCTAAGT 301 AAGAGAAGTT GTTAGTGATT TCTTCAAGTT TACTAAAATT TACATACAAA TGGATGATCT 361 CTTCTCCATT GTTTCCTTTC TTCTTGCAAA CGCGTTTTAC AAAGAGATGA CATGCTATTT 421 TCTGATTATT TTTTTCTATA GTTTTCTATT TTGTACGTCC TTGGGAGTCC GAAATGTAAA 481 ATCGGGGTAT GGTTAGTAGC GGCAGCGTTC ATTATGGTAT ATAAAAGATA AAAAGTAGGG 541 GCAAGCGAGA CAGAATGGGA TACAAGGGCA TCGTCTATAA TTATAGCTAA AAATTGTATT 601 TTAATTTGTA TTTTTTGTAA TTTTATTTCT CATTGTTTTA CTTAAAATGA ATGCGAATTA 661 GAGAAAACTT CAACGAAATG TCAAAATAAG CTCAGCAAAA TATACAATTT TAGGGAAAGC 721 GATCAGCAAC TTATTCTCCG TTGTAGATTC TTTTTCTTCA TAAATTCCAA CAATGTAACT 781 GCCAATTGAT TTTTCTCTAG ATGCTAATAA ATTGAAACTA ATGAATATAA TAACACAGGT 841 TTTGATAAAT TGTAAACTCT TACTCAAAAA AAGAATGTTA AAACTAATTT AACCTTTTTT 901 ATAAAGGCTC ATGGCGTTAA AGAGGATAGT AAAAATTCTA CAATTTTTTT AATTTAATGT 961 CACAATTGTC TTGCGAATAT AATTCTATTA ATTTTCGCTA ACTGCTGTTT TAGTACTATT 1021 TACTTTCATA TGCCCTTATA TGCAACTTTA ATTTCATGAG AATGCAATAT TTTTTGCAAT 1081 CTTTCTTGTA CTGAGCGCTT TTTTATCATT TAGTTACTCA TTATTTCATT TTTCAAGGTA 1141 AAGGGCCAAT TAAATT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

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Reference sequence