Resource data sheet
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pREP41-ccdB2

S. pombe vector with nmt1 (medium strength) promoter

Catalog number RDB06520
Resource name pREP41-ccdB2
Clone info. S. pombe vector with nmt1 (medium strength) promoter.
Gateway compatible.
Vector backbone pREP41 (Plasmid)
Size of vector backbone 10.5 kb
Selectable markers E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor Yoshida, Minoru | Matsuyama, Akihisa |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature(s). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.
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RDB06520 pREP41-ccdB2 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB06520z.seq from Depositor

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12284_A5L2p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw | Sequence: RDB12284_A5L2a.seq
>06520_12284_A5L2_nmt1Fw_E12_15.ab1
    1 GAAAATTTTA TTAAATCAGG GGAAAACGTA ACTCTCGGCT ACTGGATGGT TCAGTCACCC
   61 AACGATTACT GGGGAGAGAA AACAGGGCAA AAGCAAAGCT TAAAGGAATC CGATTGTCAT
  121 TCGGCAATGT GCAGCGAAAC TAAAAACCGG ATAATGGACC TGTTAATCGA AACATTGAAG
  181 ATAAAGGAAG AGGAATCCTG GCATATCATC AATTGAATAA GTTGAATTAA TTATTTCAAT
  241 CTCATTCTCA CTTTCTGACT TATAGTCGCT TTGTTAAAGC TAGCGTCGAC AGGCCTGGAT
  301 CCGGATATCA ACAAGTTTGT ACAAAAAAGC TGAACGAGAA ACGTAAAATG ATATAAATAT
  361 CAATATATTA AATTAGATTT TGCATAAAAA ACAGACTACA TAATACTGTA AAACACAACA
  421 TATCCAGTCA CTATGGCGGC CGCATTAGGC ACCCCAGGCT TTACACTTTA TGCTTCCGGC
  481 TCGTATAATG TGTGGATTTT GAGTTAGGAT CCGTCGAGAT TTTCAGGAGC TAAGGAAGCT
  541 AAAATGGAGA AAAAAATCAC TGGATATACC ACCGTTGATA TATCCCAATG GCATCGTAAA
  601 GAACATTTTG AGGCATTTCA GTCAGTTGCT CAATGTACCT ATAACCAGAC CGTTCAGCTG
  661 GATATTACGG CCTTTTTAAA GACCGTAAAG AAAAATAAGC ACAAGTTTTA TCCGGCCTTT
  721 ATTCACATTC TTGCCCGCCT GATGAATGCT CATCCGGAAT TCCGTATGGC AATGAAAGAC
  781 GGTGAGCTGG TGATATGGGA TAGTGTTCAC CCTTGTTACA CCGTTTTCCA TGAGCAAACT
  841 GAAACGTTTT CATCGCTCTG GAGTGAATAC CACGACGATT TCCGGCAGTT TCTACACATA
  901 TATTCGCAAG ATGTGGCGTG TTACGGTGAA AACCTGGCCC TATTTCCCTA AAGGGTTTAT
  961 TGAGAATATG TTTTTCGTCT CAGCCAATCC CTGGGTGAGT TTCACAGTTT TGATTTAACG
 1021 TATCATATGG GACACTTTCT TCGCCCCCGT TTTCAACAAT GGGCAATATT ATACGCGGCC
 1081 GACCAGTTGC TGATGGCGGC TGCGATTCAG TTCATCATGC CGATGTGAAT GCCATGCAAT
1141 GTTCGGCAGA ATGGCCTA
//
Primer: nmt1Rv | Sequence: RDB12284_A5L2b.seq
>06520_12284_A5L2_nmt1Rv_F12_18.ab1
    1 AGCGGCGGGG GCTCGAGATC TCATGATATC ACCACTTTGT ACAAGAAAGC TGAACGAGAA
   61 ACGTAAAATG ATATAAATAT CAATATATTA AATTAGATTT TGCATAAAAA ACAGACTACA
  121 TAATACTGTA AAACACAACA TATCCAGTCA CTATGGTCGA CCTGCAGACT GGCTGTGTAT
  181 AAGGGAGCCT GACATTTATA TTCCCCAGAA CATCAGGTTA ATGGCGTTTT TGATGTCATT
  241 TTCGCGGTGG CTGAGATCAG CCACTTCTTC CCCGATAACG GAGACCGGCA CACTGGCCAT
  301 ATCGGTGGTC ATCATGCGCC AGCTTTCATC CCCGATATGC ACCACCGGGT AAAGTTCACG
  361 GGAGACTTTA TCTGACAGCA GACGTGCACT GGCCAGGGGG ATCACCATCC GTCGCCCGGG
  421 CGTGTCAATA ATATCACTCT GTACATCCAC AAACAGACGA TAACGGCTCT CTCTTTTATA
  481 GGTGTAAACC TTAAACTGCA TTTCACCAGC CCCTGTTCTC GTCAGCAAAA GAGCCGTTCA
  541 TTTCAATAAA CCGGGCGACC TCAGCCATCC CTTCCTGATT TTCCGCTTTC CAGCGTTCGG
  601 CACGCAGACG ACGGGCTTCA TTCTGCATGG TTGTGCTTAC CAGACCGGAG ATATTGACAT
  661 CATATATGCC TTGAGCAACT GATAGCTGTC GCTGTCAACT GTCACTGTAA TACGCTGCTT
  721 CATAGCATAC CTCTTTTTGA CATACTTCGG GTATACATAT CAGTATATAT TCTTATACCG
  781 CAAAAATCAG CGCGCAAATA CGCATACTGT TATCTGGCTT TTAGTAAGCC GGATCCACGC
  841 GTTTACGCCC CGCCCTGCCA CTCATCGCAG TACTGTTGTA ATTCATTAAG CATTCTGCCG
  901 ACATGGAAGC CATCACAAAC GGCATGATGA ACCTGAATCG CCAGCGGCAT CAGCACCTTG
  961 TCGCCTTGCG TATAATATTT GCCCATGGGT GAAAACGGGG GGCGAAGAAG TTGTCCATAT
 1021 TGGCCACGTT TAAATCAAAA CTGGTGAAAC TCACCCAGGG ATTGGCTGAG ACGAAAAACA
 1081 TATCTCAATA AAACCTTTAG GAATCGTAGC TTTTCACGTA CACGGCACAT CCTGCATCTA
1141 TTGTGTAGCA AGCCTTCCCA GA
//
Primer: LEU2pro_F | Sequence: RDB12284_A5L2c.seq
>06520_12284_A5L2_LEU2pro_F_G12_21.ab1
    1 ACTGCAGTGA ATATTATTTA AGGACCTATT GTTTTTTCCA ATAGGTGGTT AGCAATCGTC
   61 TTACTTTCTA ACTTTTCTTA CCTTTTACAT TTCAGCAATA TATATATATA TTTCAAGGAT
  121 ATACCATTCT AATGTCTGCC CCTAAGAAGA TCGTCGTTTT GCCAGGTGAC CACGTTGGTC
  181 AAGAAATCAC AGCCGAAGCC ATTAAGGTTC TTAAAGCTAT TTCTGATGTT CGTTCCAATG
  241 TCAAGTTCGA TTTCGAAAAT CATTTAATTG GTGGTGCTGC TATCGATGCT ACAGGTGTTC
  301 CACTTCCAGA TGAGGCGCTG GAAGCCTCCA AGAAGGCTGA TGCCGTTTTG TTAGGTGCTG
  361 TGGGTGGTCC TAAATGGGGT ACCGGTAGTG TTAGACCTGA ACAAGGTTTA CTAAAAATCC
  421 GTAAAGAACT TCAATTGTAC GCCAACTTAA GACCATGTAA CTTTGCATCC GACTCTCTTT
  481 TAGACTTATC TCCAATCAAG CCACAATTTG CTAAAGGTAC TGACTTCGTT GTTGTCAGAG
  541 AATTAGTGGG AGGTATTTAC TTTGGTAAGA GAAAGGAAGA CGATGGTGAT GGTGTCGCTT
  601 GGGATAGTGA ACAATACACC GTTCCAGAAG TGCAAAGAAT CACAAGAATG GCCGCTTTCA
  661 TGGCCCTACA ACATGAGCCA CCATTGCCTA TTTGGTCCTT GGATAAAGCT AATGTTTTGG
  721 CCTCTTCAAG ATTATGGAGA AAAACTGTGG AGGAAACCAT CAAGAACGAA TTCCCTACAT
  781 TGAAGGTTCA ACATCAATTG ATTGATTCTG CCGCCATGAT CCTAGTTAAG AACCCAACCC
  841 ACCTAAATGG TATTATAATC ACCAGCAACA TGTTTGGTGA CATCATCTCC GATGAAGCCT
  901 CCGTTATCCC AGGTTTCCTT GGGTTTGTTG CCATCTGCGT CTTGGCCTCT TTGCCAGACA
  961 GACACCGCAT TGGTTTGTAC GACATGCCAC GGTCTGCTCA GATTGCTAAG AATAGTCACC
 1021 TATCGCCACT ATCTTGTCTG CTGCATGATG GTGAATGTCA ATGGAACCTG GCTGGACACG
1081 TAGCCATGAA TGCAGTTAAA CGGTTGGCAA TGCAGGAAAT CGA
//
Primer: f1ori_R3 | Sequence: RDB12284_A5L2d.seq
>06520_12284_A5L2_f1ori_R3_H12_24.ab1
    1 GGTGGGATCT TGAGTATGCG GTGTGAATAC CGCACAGATG CGTAAGGAGA AAATACCGCA
   61 TCAGGCGCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT
  121 CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA
  181 CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGAATTC CAACCTTCCA
  241 ATTCATTAAA TCGAGTCCTC CATCAGATGA TTTACAAGGT TTTGCATAGA ATCCCTAAGT
  301 AAGAGAAGTT GTTAGTGATT TCTTCAAGTT TACTAAAATT TACATACAAA TGGATGATCT
  361 CTTCTCCATT GTTTCCTTTC TTCTTGCAAA CGCGTTTTAC AAAGAGATGA CATGCTATTT
  421 TCTGATTATT TTTTTCTATA GTTTTCTATT TTGTACGTCC TTGGGAGTCC GAAATGTAAA
  481 ATCGGGGTAT GGTTAGTAGC GGCAGCGTTC ATTATGGTAT ATAAAAGATA AAAAGTAGGG
  541 GCAAGCGAGA CAGAATGGGA TACAAGGGCA TCGTCTATAA TTATAGCTAA AAATTGTATT
  601 TTAATTTGTA TTTTTTGTAA TTTTATTTCT CATTGTTTTA CTTAAAATGA ATGCGAATTA
  661 GAGAAAACTT CAACGAAATG TCAAAATAAG CTCAGCAAAA TATACAATTT TAGGGAAAGC
  721 GATCAGCAAC TTATTCTCCG TTGTAGATTC TTTTTCTTCA TAAATTCCAA CAATGTAACT
  781 GCCAATTGAT TTTTCTCTAG ATGCTAATAA ATTGAAACTA ATGAATATAA TAACACAGGT
  841 TTTGATAAAT TGTAAACTCT TACTCAAAAA AAGAATGTTA AAACTAATTT AACCTTTTTT
  901 ATAAAGGCTC ATGGCGTTAA AGAGGATAGT AAAAATTCTA CAATTTTTTT AATTTAATGT
  961 CACAATTGTC TTGCGAATAT AATTCTATTA ATTTTCGCTA ACTGCTGTTT TAGTACTATT
 1021 TACTTTCATA TGCCCTTATA TGCAACTTTA ATTTCATGAG AATGCAATAT TTTTTGCAAT
 1081 CTTTCTTGTA CTGAGCGCTT TTTTATCATT TAGTTACTCA TTATTTCATT TTTCAAGGTA
1141 AAGGGCCAAT TAAATT
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles


2019.09.27

GNP_filter3_RDBDEP_html_190927.pl