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pFFH1-ccdB2

S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 promoter

Catalog number RDB06506
Resource name pFFH1-ccdB2
Clone info. S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 promoter.
Gateway compatible.
Vector backbone pREP1 (Plasmid)
Selectable markers E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor Yoshida, Minoru | Matsuyama, Akihisa |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature(s). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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Material Transfer Agreement (MTA) [Word]
Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06506 pFFH1-ccdB2 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB06506z.seq from Depositor

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12291_A3Jm.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw | Sequence: RDB12291_A3Jma.seq
>06506_12291_A3Jm_nmt1Fw_E01_13.ab1
    1 ATGGAAAATT TAATTAAATC AAGGAAAAAA CGTAAACTCT CGGCCTACTG GATGGTTCAG
   61 TCACCCAACG ATTACTGGGG AGAGAAAACA GGGCAAAAGC AAAGCTTAAA GGAATCCGAT
  121 TGTCATTCGG CAATGTGCAG CGAAACTAAA AACCGGATAA TGGACCTGTT AATCGAAACA
  181 TTGAAGATAT ATAAAGGAAG AGGAATCCTG GCATATCATC AATTGAATAA GTTGAATTAA
  241 TTATTTCAAT CTCATTCTCA CTTTCTGACT TATAGTCGCT TTGTTAAAGC TAGCGTCGAC
  301 AGGCCTGGAT CCGGATATCA ACAAGTTTGT ACAAAAAAGC TGAACGAGAA ACGTAAAATG
  361 ATATAAATAT CAATATATTA AATTAGATTT TGCATAAAAA ACAGACTACA TAATACTGTA
  421 AAACACAACA TATCCAGTCA CTATGGCGGC CGCATTAGGC ACCCCAGGCT TTACACTTTA
  481 TGCTTCCGGC TCGTATAATG TGTGGATTTT GAGTTAGGAT CCGTCGAGAT TTTCAGGAGC
  541 TAAGGAAGCT AAAATGGAGA AAAAAATCAC TGGATATACC ACCGTTGATA TATCCCAATG
  601 GCATCGTAAA GAACATTTTG AGGCATTTCA GTCAGTTGCT CAATGTACCT ATAACCAGAC
  661 CGTTCAGCTG GATATTACGG CCTTTTTAAA GACCGTAAAA GAAAAATAAG CACAAGTTTT
  721 ATCCGGCCTT TATTCACATT CTTGCCCGCC TGATGAATGC TCATCCGGAA TTCCCGTATG
  781 GCAAATGAAA AGACGGTGAG CTGGTGATAT GGGATAGTGT TCACCCCTTG TTACACCGTT
  841 TTCCATGAGC AAAACTGAAA ACGTTTTCAT CGCTCTGGAG TTGAATACCA CGACGATTTC
  901 CCGGCAGTTT CTACACATAA TATTCGCAAA GATGGTGGCG TGTTACGGTG AAAAACCCTG
  961 GGCCCTAATT TCCCTAAAGG GGTTTAATTT GAGAATATGT TTTTTCGGTC TCAGCCAAAT
 1021 CCCCTGGGGT GAGTTTCACC CAGTTTTTGA TTTAAAACGT GGCCCAATAA TGGGAACAAA
 1081 TTTTCTTCCG CCCCCGTTTT CACCATGGGG CCAAAATAAA TTATTAACGC AAAGGCCGAA
 1141 CAAAGGGTGG CTGGAATGGC CCGCGTGGCG CGAATCCAGG TCAATTCATG GCCGGTTCTG
1201 TGTGAATGGG CTTCCCAAGT GTTCGGGGGC CGAGGAAAAT TGCCTCTTAT ATGATG
//
Primer: nmt1Rv | Sequence: RDB12291_A3Jmb.seq
>06506_12291_A3Jm_nmt1Rv_F01_16.ab1
    1 AGCGCCCCGG GTCTGAGATC TCAGTGGTGA TGGTGGTGAT GTTTGTCATC GTCGTCCTTG
   61 TAGTCCATGG GTTTATCGTC ATCATCCTTA TAATCCATGA TATCAACCAC TTTGTACAAG
  121 AAAGCTGAAC GAGAAACGTA AAATGATATA AATATCAATA TATTAAATTA GATTTTGCAT
  181 AAAAAACAGA CTACATAATA CTGTAAAACA CAACATATCC AGTCACTATG GTCGACCTGC
  241 AGACTGGCTG TGTATAAGGG AGCCTGACAT TTATATTCCC CAGAACATCA GGTTAATGGC
  301 GTTTTTGATG TCATTTTCGC GGTGGCTGAG ATCAGCCACT TCTTCCCCGA TAACGGAGAC
  361 CGGCACACTG GCCATATCGG TGGTCATCAT GCGCCAGCTT TCATCCCCGA TATGCACCAC
  421 CGGGTAAAGT TCACGGGAGA CTTTATCTGA CAGCAGACGT GCACTGGCCA GGGGGATCAC
  481 CATCCGTCGC CCGGGCGTGT CAATAATATC ACTCTGTACA TCCACAAACA GACGATAACG
  541 GCTCTCTCTT TTATAGGTGT AAACCTTAAA CTGCATTTCA CCAGCCCCTG TTCTCGTCAG
  601 CAAAAGAGCC GTTCATTTCA ATAAACCGGG CGACCTCAGC CATCCCTTCC TGATTTTCCG
  661 CTTTCCAGCG TTCGGCACGC AGACGACGGG CTTCATTCTG CATGGTTGTG CTTACCAGAC
  721 CGGAGATATT GACATCATAT ATGCCTTGAG CAACTGATAG CTGTCGCTGT CAACTGTCAC
  781 TGTAATACGC TGCTTCATAG CATACCTCTT TTTGACATAC TTCGGGTATA CATATCAGTA
  841 TATATTCTTA TACCGCAAAA ATCAGCGCGC AAAATACGCA TACTGTTATC TGGCTTTTAG
  901 TAAGCCGGAT CCAGATCTTT ACGCCCCGCC CTGCCACTCA TCGCAGTACT GTTGTAATTC
  961 ATTAAGCATT CTGCCGACAT GGAAGCCATC ACAAACGGCA TGATGAAACC TGAATCGCCA
 1021 GCGGCATCAG CACCTTGTCG CCCTTGCGTA TAATATTTTG CCCATGGTGA AACGGGGGCG
 1081 GAGAGTGTCC ATATTGCCAC GTTTAATTCA ACTTGTGACT TCACCAAGGA ATTGCTTGAA
1141 GAACGAAAAA CATATATTTC CTTCCGA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles


2019.09.27

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