Resource data sheet
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pYFH1-ccdB2 rfC

S. pombe 3 ter end YFP-FLAG-His6-tagged vector with nmt1 promoter

Catalog number RDB06504
Resource name pYFH1-ccdB2 rfC
Clone info. S. pombe 3 ter end YFP-FLAG-His6-tagged vector with nmt1 promoter.
Gateway compatible.
Vector backbone pREP1 (Plasmid)
Selectable markers E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor Yoshida, Minoru | Matsuyama, Akihisa |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature(s). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
Ordering Please visit Information of Request for Distribution. 
Order form 
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Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.
Please use Schedule A (GFP-A) additionally.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06504 pYFH1-ccdB2 rfC DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB06504z.seq from Depositor

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12333_A3Jm.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw | Sequence: RDB12333_A3Jma.seq
>06504_12333_A3Jm_nmt1Fw_C02_08.ab1
    1 TAATTTTTTT ATTTAAATCA GGAAAACGTA ACTCTCGGCT ACTGGATGGT TCAGTCACCC
   61 AACGATTACT GGGGAGAGAA AACAGGGCAA AAGCAAAGCT TAAAGGAATC CGATTGTCAT
  121 TCGGCAATGT GCAGCGAAAC TAAAAACCGG ATAATGGACC TGTTAATCGA AACATTGAAG
  181 ATATATAAAG GAAGAGGAAT CCTGGCATAT CATCAATTGA ATAAGTTGAA TTAATTATTT
  241 CAATCTCATT CTCACTTTCT GACTTATAGT CGCTTTGTTA AAGCTAGCGT CGACAGGCCT
  301 GGATCCGGAT ATCAAACAAG TTTGTACAAA AAAGCTGAAC GAGAAACGTA AAATGATATA
  361 AATATCAATA TATTAAATTA GATTTTGCAT AAAAAACAGA CTACATAATA CTGTAAAACA
421 CAACATATCC AGTCACTATG GCGGCCGCGG GTGAT
//
Primer: nmt1Rv | Sequence: RDB12333_A3Jmb.seq
>06504_12333_A3Jm_nmt1Rv_D02_11.ab1
    1 ATCCCCCCGG GGCCTCGAGA TCTCAGTGGT GATGGTGGTG ATGTTTGTCA ATCGTCGTCC
   61 TTGTAGTCCA TGGGCTTGTA CAGCTCGTCC ATGCCGAGAG TGATCCCGGC GGCGGTCACG
  121 AACTCCAGCA GGACCATGTG ATCGCGCTTC TCGTTGGGGT CTTTGCTCAG GGCGGACTGG
  181 TAGCTCAGGT AGTGGTTGTC GGGCAGCAGC ACGGGGCCGT CGCCGATGGG GGTGTTCTGC
  241 TGGTAGTGGT CGGCGAGCTG CACGCTGCCG TCCTCGATGT TGTGGCGGAT CTTGAAGTTC
  301 ACCTTGATGC CGTTCTTCTG CTTGTCGGCC ATGATATAGA CGTTGTGGCT GTTGTAGTTG
  361 TACTCCAGCT TGTGCCCCAG GATGTTGCCG TCCTCCTTGA AGTCGATGCC CTTCAGCTCG
  421 ATGCGGTTCA CCAGGGTGTC GCCCTCGAAC TTCACCTCGG CGCGGGTCTT GTAGTTGCCG
  481 TCGTCCTTGA AGAAGATGGT GCGCTCCTGG ACGTAGCCTT CGGGCATGGC GGACTTGAAG
  541 AAGTCGTGCT GCTTCATGTG GTCGGGGTAG CGGGCGAAGC ACTGCAGGCC GTAGCCGAAG
  601 GTGGTCACGA GGGTGGGCCA GGGCACGGGC AGCTTGCCGG TGGTGCAGAT GAACTTCAGG
661 GTCAGCTTGC CGTAG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles


2019.09.27

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