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pDUAL-GFH31c

S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with cam1 promoter

Catalog number RDB06498
Resource name pDUAL-GFH31c
Clone info. S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with cam1 promoter. Multicopy marker, ura4+; Integration marker, leu1+.
Gateway compatible.
Vector backbone pDUAL (Plasmid)
Selectable markers E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Reference of nucleotide sequence AB364148 (DDBJ accession number)
Depositor Yoshida, Minoru | Matsuyama, Akihisa |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., A., Yeast, 25 (5): 371-376 (2008)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
Ordering Please visit Information of Request for Distribution. 
Order form 
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Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.
Please use Schedule A (GFP-A) additionally.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06498 pDUAL-GFH31c DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB06498z.seq from Depositor

Original reference

original Matsuyama, A., A series of promoters for constitutive expression of heterologous genes in fission yeast. Yeast, 25 (5):371-376 (2008). PMID 18437702.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13143_A5C2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: ADH_rev | Sequence: RDB13143_A5C2a.seq
>06498_13143_A5C2_ADHrev_F11_17.ab1
    1 GGAGACTCTC AGGATAAGAT TTTCGTTTTA AACCTAAGAG TCACTTTAAA ATTTGTATAC
   61 ACTTATTTTT TTTATAACTT ATTTAATAAT AAAAATCATA AATCATAAGA AATTCGCTTA
  121 TTTAGAAGTG GCGCGCCGGA TCCTCTAGAG TCGAGATCTC AGTGGTGATG GTGGTGATGT
  181 TTGTCATCGT CGTCCTTGTA GTCCATGGGT TTGTATAGTT CATCCATGCC ATGTGTAATC
  241 CCAGCAGCTG TTACAAACTC AAGAAGGACC ATGTGGTCTC TCTTTTCGTT GGGATCTTTC
  301 GAAAGGGCAG ATTGTGTGGA CAGGTAATGG TTGTCTGGTA AAAGGACAGG GCCATCGCCA
  361 ATTGGAGTAT TTTGTTGATA ATGGTCTGCT AGTTGAACGC TTCCATCTTC AATGTTGTGT
  421 CTAATTTTGA AGTTAACTTT GATTCCATTC TTTTGTTTGT CTGCCATGAT GTATACATTG
  481 TGTGAGTTAT AGTTGTATTC CAATTTGTGT CCAAGAATGT TTCCATCTTC TTTAAAATCA
  541 ATACCTTTTA ACTCGATTCT ATTAACAAGG GTATCACCTT CAAACTTGAC TTCAGCACGT
  601 GTCTTGTAGT TCCCGTCATC TTTGAAAAAT ATAGTTCTTT CCTGTACATA GCCTTCGGGC
  661 ATGGCACTCT TGAAAAAGTC ATGCTGTTTC ATGTGATCTG GGTATCTTGA AAAGCATTGA
  721 ACACCATAAC AGAAAGTAGT GACAAGTGTT GGCCACGGAA CAGGTAGTTT TCCAGTAGTG
  781 CAAATAAATT TAAGGGTAAG TTTTCCGTAT GTTGCATCAC CTTCACCCTC TCCACTGACA
  841 GAAAATTTGT GCCCATTAAC ATCACCATCT AATTCAACAA GAATTGGGAC AACTCCAGTG
  901 AAAAGTTCTT CTCCTTTACT CATGATATCA ACCACTTTGT ACAAGAAAGC TGAACGAGAA
  961 ACGTAAAATG ATATAAATAT CAATATATTA AATTAGATTT TGCATAAAAA AACAGACTAC
 1021 ATAATACTGT AAAACACACA TATCCAGTCA CTATGGGTCG ACCCTGCAGA CTGACTGTGT
 1081 ATAGGAGCCC TGACATTTAT ATTCCCCAGA ACATCAAGGC TAAATGGACG GATTTTGATT
1141 CATTCGCAGA ATGACCTGGT AGAAA
//
Primer: Cm_N_rev | Sequence: RDB13143_A5C2b.seq
>06498_13143_A5C2_Cm_N_rev_B12_06.ab1
    1 GGTAAGCCTG ATGTTCTTTA CGATGCCATT GGGATATATC AACGGTGGTA TATCCAGTGA
   61 TTTTTTTCTC CATTTTAGCT TCCTTAGCTC CTGAAAATCT CGACGGATCC TAACTCAAAA
  121 TCCACACATT ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGCGGCCG
  181 CCATAGTGAC TGGATATGTT GTGTTTTACA GTATTATGTA GTCTGTTTTT TATGCAAAAT
  241 CTAATTTAAT ATATTGATAT TTATATCATT TTACGTTTCT CGTTCAGCTT TTTTGTACAA
  301 ACTTGTTGAT ATCCGGATCC AGGCCTGTCG ACGCTAGCAT CAGTAACTCT AAAGTCCTTC
  361 TATTCCTTTT TTTAACCTTT ATTTTTGGCG GTTAGTGTTT TGCTCTAGTT ACGTTAAAGT
  421 GCCTTGGAGA AATGAGGACT ACCGTTGTCG CTTCCTAATG TTGCAAGTCC TACCATCAAC
  481 AGTGATGTCA ACAAACAATG AGAGTTTATT ACATTATCGT ATATGCTCTA ACAAACCAGT
  541 AACGTACTTG CTATAAACCG CACTAAAACC ACTAAACGGC GGATGAAGGA ACTTTTTCAA
  601 TGTCAGCTAC GCATTTATTG GTATCTATTT AACGACACCT AGAACTGCCG TGTGGTTTAC
  661 TTGTGACCTA TTGTATTATG GTACACAGGT AAATACATTT AGTAACTTAG AATGATATTC
  721 TGATATAGAG GTTATATTGT TACCTATCAC CGAATTAGCA TAAAAGTGAA TAGAGAAACG
  781 AGGTTTTGTT TATTTCAATT CATAAAATTT AAGCATTCTA TTATTTTTTT TGTTATTCAT
  841 TTTATAACTC TTAGTAATCT ACCTAAGTAA CTTATCATGT ACAGCTTGAC AGAGCAAATC
  901 AAACATGTGA TAATTAGTGA TACACAACGT AGTATGCTAT AGCGCATGCA AGCTTGGCGT
  961 AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA
 1021 TACGAGCCGG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA
 1081 TTTACTGCGA TGCGCCTCAC TGCCCGCTTT TCCAGTCAGC AATCTGGTCT GCTAGCCTGC
1141 ATAATGAATC GGCCACGACG CGGGGCAAAA AGGTCG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Matsuyama, A., A series of promoters for constitutive expression of heterologous genes in fission yeast. Yeast, 25 (5):371-376 (2008). PMID 18437702.
reference Snaith, H.A., New and old reagents for fluorescent protein tagging of microtubules in fission yeast; experimental and critical evaluation. Methods Cell Biol., 97: 147-172 (2010). PMID 20719270.

2019.09.27

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