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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pDUAL-GFH31c

S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with cam1 promoter

Catalog number RDB06498
Resource name pDUAL-GFH31c
Clone info. S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with cam1 promoter. Multicopy marker, ura4+; Integration marker, leu1+.
Comment Gateway compatible.
Vector backbone pDUAL (Plasmid)
Selectable markers E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Reference of insert sequence AB364148 (DDBJ accession number)
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., A., Yeast, 25 (5): 371-376 (2008)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
            Order form 
            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
            Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast, 25 (5): 371-376 (2008)) の引用と、寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。
            備考 このクローンの増殖にはSurvival2 (Invitrogen) あるいは同等の宿主を使用してください。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB06498 pDUAL-GFH31c DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB06498z.seq

            Original reference

            original Matsuyama, A., A series of promoters for constitutive expression of heterologous genes in fission yeast. Yeast, 25 (5):371-376 (2008). PMID 18437702.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB13143_A5C2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: ADH_rev
            Sequence file: RDB13143_A5C2a.seq check
            >06498_13143_A5C2_ADHrev_F11_17.ab1
                1 GGAGACTCTC AGGATAAGAT TTTCGTTTTA AACCTAAGAG TCACTTTAAA ATTTGTATAC
               61 ACTTATTTTT TTTATAACTT ATTTAATAAT AAAAATCATA AATCATAAGA AATTCGCTTA
              121 TTTAGAAGTG GCGCGCCGGA TCCTCTAGAG TCGAGATCTC AGTGGTGATG GTGGTGATGT
              181 TTGTCATCGT CGTCCTTGTA GTCCATGGGT TTGTATAGTT CATCCATGCC ATGTGTAATC
              241 CCAGCAGCTG TTACAAACTC AAGAAGGACC ATGTGGTCTC TCTTTTCGTT GGGATCTTTC
              301 GAAAGGGCAG ATTGTGTGGA CAGGTAATGG TTGTCTGGTA AAAGGACAGG GCCATCGCCA
              361 ATTGGAGTAT TTTGTTGATA ATGGTCTGCT AGTTGAACGC TTCCATCTTC AATGTTGTGT
              421 CTAATTTTGA AGTTAACTTT GATTCCATTC TTTTGTTTGT CTGCCATGAT GTATACATTG
              481 TGTGAGTTAT AGTTGTATTC CAATTTGTGT CCAAGAATGT TTCCATCTTC TTTAAAATCA
              541 ATACCTTTTA ACTCGATTCT ATTAACAAGG GTATCACCTT CAAACTTGAC TTCAGCACGT
              601 GTCTTGTAGT TCCCGTCATC TTTGAAAAAT ATAGTTCTTT CCTGTACATA GCCTTCGGGC
              661 ATGGCACTCT TGAAAAAGTC ATGCTGTTTC ATGTGATCTG GGTATCTTGA AAAGCATTGA
              721 ACACCATAAC AGAAAGTAGT GACAAGTGTT GGCCACGGAA CAGGTAGTTT TCCAGTAGTG
              781 CAAATAAATT TAAGGGTAAG TTTTCCGTAT GTTGCATCAC CTTCACCCTC TCCACTGACA
              841 GAAAATTTGT GCCCATTAAC ATCACCATCT AATTCAACAA GAATTGGGAC AACTCCAGTG
              901 AAAAGTTCTT CTCCTTTACT CATGATATCA ACCACTTTGT ACAAGAAAGC TGAACGAGAA
              961 ACGTAAAATG ATATAAATAT CAATATATTA AATTAGATTT TGCATAAAAA AACAGACTAC
             1021 ATAATACTGT AAAACACACA TATCCAGTCA CTATGGGTCG ACCCTGCAGA CTGACTGTGT
             1081 ATAGGAGCCC TGACATTTAT ATTCCCCAGA ACATCAAGGC TAAATGGACG GATTTTGATT
            1141 CATTCGCAGA ATGACCTGGT AGAAA
            //
            Primer: Cm_N_rev
            Sequence file: RDB13143_A5C2b.seq check
            >06498_13143_A5C2_Cm_N_rev_B12_06.ab1
                1 GGTAAGCCTG ATGTTCTTTA CGATGCCATT GGGATATATC AACGGTGGTA TATCCAGTGA
               61 TTTTTTTCTC CATTTTAGCT TCCTTAGCTC CTGAAAATCT CGACGGATCC TAACTCAAAA
              121 TCCACACATT ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGCGGCCG
              181 CCATAGTGAC TGGATATGTT GTGTTTTACA GTATTATGTA GTCTGTTTTT TATGCAAAAT
              241 CTAATTTAAT ATATTGATAT TTATATCATT TTACGTTTCT CGTTCAGCTT TTTTGTACAA
              301 ACTTGTTGAT ATCCGGATCC AGGCCTGTCG ACGCTAGCAT CAGTAACTCT AAAGTCCTTC
              361 TATTCCTTTT TTTAACCTTT ATTTTTGGCG GTTAGTGTTT TGCTCTAGTT ACGTTAAAGT
              421 GCCTTGGAGA AATGAGGACT ACCGTTGTCG CTTCCTAATG TTGCAAGTCC TACCATCAAC
              481 AGTGATGTCA ACAAACAATG AGAGTTTATT ACATTATCGT ATATGCTCTA ACAAACCAGT
              541 AACGTACTTG CTATAAACCG CACTAAAACC ACTAAACGGC GGATGAAGGA ACTTTTTCAA
              601 TGTCAGCTAC GCATTTATTG GTATCTATTT AACGACACCT AGAACTGCCG TGTGGTTTAC
              661 TTGTGACCTA TTGTATTATG GTACACAGGT AAATACATTT AGTAACTTAG AATGATATTC
              721 TGATATAGAG GTTATATTGT TACCTATCAC CGAATTAGCA TAAAAGTGAA TAGAGAAACG
              781 AGGTTTTGTT TATTTCAATT CATAAAATTT AAGCATTCTA TTATTTTTTT TGTTATTCAT
              841 TTTATAACTC TTAGTAATCT ACCTAAGTAA CTTATCATGT ACAGCTTGAC AGAGCAAATC
              901 AAACATGTGA TAATTAGTGA TACACAACGT AGTATGCTAT AGCGCATGCA AGCTTGGCGT
              961 AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA
             1021 TACGAGCCGG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA
             1081 TTTACTGCGA TGCGCCTCAC TGCCCGCTTT TCCAGTCAGC AATCTGGTCT GCTAGCCTGC
            1141 ATAATGAATC GGCCACGACG CGGGGCAAAA AGGTCG
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Matsuyama, A., A series of promoters for constitutive expression of heterologous genes in fission yeast. Yeast, 25 (5):371-376 (2008). PMID 18437702.
            reference Snaith, H.A., New and old reagents for fluorescent protein tagging of microtubules in fission yeast; experimental and critical evaluation. Methods Cell Biol., 97: 147-172 (2010). PMID 20719270.

            2022.07.13

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