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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pDUAL-GFH31c (#RDB06498)

S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with cam1 promoter

Clone info. S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with cam1 promoter. Multicopy marker, ura4+; Integration marker, leu1+.
Comment Gateway compatible.
Vector backbone pDUAL (RDB06178) (Plasmid)
Selectable markers E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Reference of insert sequence AB364148 (DDBJ accession number)
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
 
Sequence RDB06498z.seq

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., A., Yeast, 25 (5): 371-376 (2008)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast, 25 (5): 371-376 (2008)) の引用と、寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
備考 このクローンの増殖にはSurvival2 (Invitrogen) あるいは同等の宿主を使用してください。

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RDB06498 pDUAL-GFH31c DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pDUAL-GFH31c was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06498).

Reference section:

Matsuyama, A., Shirai, A., Yoshida, M., A series of promoters for constitutive expression of heterologous genes in fission yeast. Yeast, 25 (5):371-376 (2008). PMID 18437702. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13143_A5C2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: ADH_rev
Sequence file: RDB13143_A5C2a.seq check
>06498_13143_A5C2_ADHrev_F11_17.ab1
    1 GGAGACTCTC AGGATAAGAT TTTCGTTTTA AACCTAAGAG TCACTTTAAA ATTTGTATAC
   61 ACTTATTTTT TTTATAACTT ATTTAATAAT AAAAATCATA AATCATAAGA AATTCGCTTA
  121 TTTAGAAGTG GCGCGCCGGA TCCTCTAGAG TCGAGATCTC AGTGGTGATG GTGGTGATGT
  181 TTGTCATCGT CGTCCTTGTA GTCCATGGGT TTGTATAGTT CATCCATGCC ATGTGTAATC
  241 CCAGCAGCTG TTACAAACTC AAGAAGGACC ATGTGGTCTC TCTTTTCGTT GGGATCTTTC
  301 GAAAGGGCAG ATTGTGTGGA CAGGTAATGG TTGTCTGGTA AAAGGACAGG GCCATCGCCA
  361 ATTGGAGTAT TTTGTTGATA ATGGTCTGCT AGTTGAACGC TTCCATCTTC AATGTTGTGT
  421 CTAATTTTGA AGTTAACTTT GATTCCATTC TTTTGTTTGT CTGCCATGAT GTATACATTG
  481 TGTGAGTTAT AGTTGTATTC CAATTTGTGT CCAAGAATGT TTCCATCTTC TTTAAAATCA
  541 ATACCTTTTA ACTCGATTCT ATTAACAAGG GTATCACCTT CAAACTTGAC TTCAGCACGT
  601 GTCTTGTAGT TCCCGTCATC TTTGAAAAAT ATAGTTCTTT CCTGTACATA GCCTTCGGGC
  661 ATGGCACTCT TGAAAAAGTC ATGCTGTTTC ATGTGATCTG GGTATCTTGA AAAGCATTGA
  721 ACACCATAAC AGAAAGTAGT GACAAGTGTT GGCCACGGAA CAGGTAGTTT TCCAGTAGTG
  781 CAAATAAATT TAAGGGTAAG TTTTCCGTAT GTTGCATCAC CTTCACCCTC TCCACTGACA
  841 GAAAATTTGT GCCCATTAAC ATCACCATCT AATTCAACAA GAATTGGGAC AACTCCAGTG
  901 AAAAGTTCTT CTCCTTTACT CATGATATCA ACCACTTTGT ACAAGAAAGC TGAACGAGAA
  961 ACGTAAAATG ATATAAATAT CAATATATTA AATTAGATTT TGCATAAAAA AACAGACTAC
 1021 ATAATACTGT AAAACACACA TATCCAGTCA CTATGGGTCG ACCCTGCAGA CTGACTGTGT
 1081 ATAGGAGCCC TGACATTTAT ATTCCCCAGA ACATCAAGGC TAAATGGACG GATTTTGATT
1141 CATTCGCAGA ATGACCTGGT AGAAA
//
Primer: Cm_N_rev
Sequence file: RDB13143_A5C2b.seq check
>06498_13143_A5C2_Cm_N_rev_B12_06.ab1
    1 GGTAAGCCTG ATGTTCTTTA CGATGCCATT GGGATATATC AACGGTGGTA TATCCAGTGA
   61 TTTTTTTCTC CATTTTAGCT TCCTTAGCTC CTGAAAATCT CGACGGATCC TAACTCAAAA
  121 TCCACACATT ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGCGGCCG
  181 CCATAGTGAC TGGATATGTT GTGTTTTACA GTATTATGTA GTCTGTTTTT TATGCAAAAT
  241 CTAATTTAAT ATATTGATAT TTATATCATT TTACGTTTCT CGTTCAGCTT TTTTGTACAA
  301 ACTTGTTGAT ATCCGGATCC AGGCCTGTCG ACGCTAGCAT CAGTAACTCT AAAGTCCTTC
  361 TATTCCTTTT TTTAACCTTT ATTTTTGGCG GTTAGTGTTT TGCTCTAGTT ACGTTAAAGT
  421 GCCTTGGAGA AATGAGGACT ACCGTTGTCG CTTCCTAATG TTGCAAGTCC TACCATCAAC
  481 AGTGATGTCA ACAAACAATG AGAGTTTATT ACATTATCGT ATATGCTCTA ACAAACCAGT
  541 AACGTACTTG CTATAAACCG CACTAAAACC ACTAAACGGC GGATGAAGGA ACTTTTTCAA
  601 TGTCAGCTAC GCATTTATTG GTATCTATTT AACGACACCT AGAACTGCCG TGTGGTTTAC
  661 TTGTGACCTA TTGTATTATG GTACACAGGT AAATACATTT AGTAACTTAG AATGATATTC
  721 TGATATAGAG GTTATATTGT TACCTATCAC CGAATTAGCA TAAAAGTGAA TAGAGAAACG
  781 AGGTTTTGTT TATTTCAATT CATAAAATTT AAGCATTCTA TTATTTTTTT TGTTATTCAT
  841 TTTATAACTC TTAGTAATCT ACCTAAGTAA CTTATCATGT ACAGCTTGAC AGAGCAAATC
  901 AAACATGTGA TAATTAGTGA TACACAACGT AGTATGCTAT AGCGCATGCA AGCTTGGCGT
  961 AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA
 1021 TACGAGCCGG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA
 1081 TTTACTGCGA TGCGCCTCAC TGCCCGCTTT TCCAGTCAGC AATCTGGTCT GCTAGCCTGC
1141 ATAATGAATC GGCCACGACG CGGGGCAAAA AGGTCG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Matsuyama, A., A series of promoters for constitutive expression of heterologous genes in fission yeast. Yeast, 25 (5):371-376 (2008). PMID 18437702. [link to RRC of NBRP]
reference Snaith, H.A., New and old reagents for fluorescent protein tagging of microtubules in fission yeast; experimental and critical evaluation. Methods Cell Biol., 97: 147-172 (2010). PMID 20719270. [link to RRC of NBRP]