RDB06497 - pDUAL-GFH21c

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pDUAL-GFH21c (#RDB06497)

S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with tub1 promoter

Clone info.	S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with tub1 promoter. Multicopy marker, ura4+; Integration marker, leu1+.
Comment	Gateway compatible.
Vector backbone	pDUAL (RDB06178) (Plasmid)
Selectable markers	E. coli: Amp^r, Cm^r, ccdB.
Growth remarks	Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Reference of insert sequence	AB364147.1 (DDBJ accession number)
Depositor\|Developer	Yoshida, Minoru \| Matsuyama, Akihisa \|

Sequence	RDB06497z.seq

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature(s). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.

Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature(s). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
Remarks	Use Survival2 (Invitrogen) or equivalent to amplify this clone.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献の引用と、寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
備考	このクローンの増殖にはSurvival2 (Invitrogen) あるいは同等の宿主を使用してください。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB06497	pDUAL-GFH21c	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pDUAL-GFH21c was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06497).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB12325_A3Kl.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Sequence file: RDB12325_A3Kla.seq
>RDB12325_Cm_N_rev_B12_06.ab1 1 CGTGGTCCTG ATGTTCTTTA CGATGCCATT GGGATATATC AACGGTGGTA TATCCAGTGA 61 TTTTTTTCTC CATTTTAGCT TCCTTAGCTC CTGAAAATCT CGACGGATCC TAACTCAAAA 121 TCCACACATT ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGCGGCCG 181 CCATAGTGAC TGGATATGTT GTGTTTTACA GTATTATGTA GTCTGTTTTT TATGCAAAAT 241 CTAATTTAAT ATATTGATAT TTATATCATT TTACGTTTCT CGTTCAGCTT TTTTGTACAA 301 ACTTGTTGAT ATCCGGATCC AGGCCTGTCG ACGCTAGCGA ACGTCTTTCC AGGGTTTCTA 361 CGAGGTTTTT GGTGTTTGGT TTGTAGTGAA GTGCGGTGTA CACTTAATCA AAACAATTCC 421 TTAATAAAGA AGTCTAACGA TATTTATTGA TGATACGGAA GGAAGTATGC TAAACATGTA 481 AATAGACATT AAAATAATGC ATTGCTTGCA AAAAGAAGCT TACTCAGTCA GGTGTTTTAA 541 GCTTTCTAAT CATAAAAAGA TTAGGATCTT CATCAATTTC ACCTTTTATT TTCTCAATTT 601 GTAAGTGAAA GATTGGATTG GTAAATTTCC ATTAAAACTC AACATGTG //
Primer: Sequence file: RDB12325_A3Klb.seq
>RDB12325_ADH_rev_D12_12.ab1 1 TGGGAGTCTC AAGAATAAGA ATTTTCGTTT TAAACCTAAG AGTCACTTTA AAATTTGTAT 61 ACACTTATTT TTTTTATAAC TTATTTAATA ATAAAAATCA TAAATCATAA GAAATTCGCT 121 TATTTAGAAG TGGCGCGCCG GATCCTCTAG AGTCGAGATC TCAGTGGTGA TGGTGGTGAT 181 GTTTGTCATC GTCGTCCTTG TAGTCCATGG GTTTGTATAG TTCATCCATG CCATGTGTAA 241 TCCCAGCAGC TGTTACAAAC TCAAGAAGGA CCATGTGGTC TCTCTTTTCG TTGGGATCTT 301 TCGAAAGGGC AGATTGTGTG GACAGGTAAT GGTTGTCTGG TAAAAGGACA GGGCCATCGC 361 CAATTGGAGT ATTTTGTTGA TAATGGTCTG CTAGTTGAAC GCTTCCATCT TCAATGTTGT 421 GTCTAATTTT GAAGTTAACT TTGATTCCAT TCTTTTGTTT GTCTGCCATG ATGTATACAT 481 TGTGTGAGTT ATAGTTGTAT TCCAATTTGT GTCCAAGAAT GTTTCCATCT TCTTTAAAAT 541 CAATACCTTT TAACTCGATT CTATTAACAA GGGTATCACC TTCAAACTTG ACTTCAGCAC 601 GTGTCTTGTA GTTCCCGTCA TCTTTGAAAA ATATAGTTCT TTCCTGTACA TAGCCTTCGG 661 GCATGGCACT CTTG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

Resource search

Link

Other resources in our bank

External Database

Reference sequence