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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pDUAL-GFH21c

S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with tub1 promoter

Catalog number RDB06497
Resource name pDUAL-GFH21c
Clone info. S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with tub1 promoter. Multicopy marker, ura4+; Integration marker, leu1+.
Comment Gateway compatible.
Vector backbone pDUAL (Plasmid)
Selectable markers E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Reference of insert sequence AB364147.1 (DDBJ accession number)
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature(s). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
            Order form 
            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
            Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献の引用と、寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。
            備考 このクローンの増殖にはSurvival2 (Invitrogen) あるいは同等の宿主を使用してください。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB06497 pDUAL-GFH21c DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB06497z.seq

            Original reference

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB12325_A3Kl.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer:
            Sequence file: RDB12325_A3Kla.seq check
            >RDB12325_Cm_N_rev_B12_06.ab1
                1 CGTGGTCCTG ATGTTCTTTA CGATGCCATT GGGATATATC AACGGTGGTA TATCCAGTGA
               61 TTTTTTTCTC CATTTTAGCT TCCTTAGCTC CTGAAAATCT CGACGGATCC TAACTCAAAA
              121 TCCACACATT ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGCGGCCG
              181 CCATAGTGAC TGGATATGTT GTGTTTTACA GTATTATGTA GTCTGTTTTT TATGCAAAAT
              241 CTAATTTAAT ATATTGATAT TTATATCATT TTACGTTTCT CGTTCAGCTT TTTTGTACAA
              301 ACTTGTTGAT ATCCGGATCC AGGCCTGTCG ACGCTAGCGA ACGTCTTTCC AGGGTTTCTA
              361 CGAGGTTTTT GGTGTTTGGT TTGTAGTGAA GTGCGGTGTA CACTTAATCA AAACAATTCC
              421 TTAATAAAGA AGTCTAACGA TATTTATTGA TGATACGGAA GGAAGTATGC TAAACATGTA
              481 AATAGACATT AAAATAATGC ATTGCTTGCA AAAAGAAGCT TACTCAGTCA GGTGTTTTAA
              541 GCTTTCTAAT CATAAAAAGA TTAGGATCTT CATCAATTTC ACCTTTTATT TTCTCAATTT
            601 GTAAGTGAAA GATTGGATTG GTAAATTTCC ATTAAAACTC AACATGTG
            //
            Primer:
            Sequence file: RDB12325_A3Klb.seq check
            >RDB12325_ADH_rev_D12_12.ab1
                1 TGGGAGTCTC AAGAATAAGA ATTTTCGTTT TAAACCTAAG AGTCACTTTA AAATTTGTAT
               61 ACACTTATTT TTTTTATAAC TTATTTAATA ATAAAAATCA TAAATCATAA GAAATTCGCT
              121 TATTTAGAAG TGGCGCGCCG GATCCTCTAG AGTCGAGATC TCAGTGGTGA TGGTGGTGAT
              181 GTTTGTCATC GTCGTCCTTG TAGTCCATGG GTTTGTATAG TTCATCCATG CCATGTGTAA
              241 TCCCAGCAGC TGTTACAAAC TCAAGAAGGA CCATGTGGTC TCTCTTTTCG TTGGGATCTT
              301 TCGAAAGGGC AGATTGTGTG GACAGGTAAT GGTTGTCTGG TAAAAGGACA GGGCCATCGC
              361 CAATTGGAGT ATTTTGTTGA TAATGGTCTG CTAGTTGAAC GCTTCCATCT TCAATGTTGT
              421 GTCTAATTTT GAAGTTAACT TTGATTCCAT TCTTTTGTTT GTCTGCCATG ATGTATACAT
              481 TGTGTGAGTT ATAGTTGTAT TCCAATTTGT GTCCAAGAAT GTTTCCATCT TCTTTAAAAT
              541 CAATACCTTT TAACTCGATT CTATTAACAA GGGTATCACC TTCAAACTTG ACTTCAGCAC
              601 GTGTCTTGTA GTTCCCGTCA TCTTTGAAAA ATATAGTTCT TTCCTGTACA TAGCCTTCGG
            661 GCATGGCACT CTTG
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles


            2022.07.13

            GNP_filter3_RDBDEP_html_220713.pl