Resource data sheet
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pDUAL-GFH21c

S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with tub1 promoter

Catalog number RDB06497
Resource name pDUAL-GFH21c
Clone info. S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with tub1 promoter. Multicopy marker, ura4+; Integration marker, leu1+.
Gateway compatible.
Vector backbone pDUAL (Plasmid)
Selectable markers E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Reference of nucleotide sequence AB364147.1 (DDBJ accession number)
Depositor Yoshida, Minoru | Matsuyama, Akihisa |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature(s). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
Ordering Please visit Information of Request for Distribution. 
Order form 
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Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.
Please use Schedule A (GFP-A) additionally.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06497 pDUAL-GFH21c DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB06497z.seq from Depositor

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12325_A3Kl.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: | Sequence: RDB12325_A3Kla.seq
>RDB12325_Cm_N_rev_B12_06.ab1
    1 CGTGGTCCTG ATGTTCTTTA CGATGCCATT GGGATATATC AACGGTGGTA TATCCAGTGA
   61 TTTTTTTCTC CATTTTAGCT TCCTTAGCTC CTGAAAATCT CGACGGATCC TAACTCAAAA
  121 TCCACACATT ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGCGGCCG
  181 CCATAGTGAC TGGATATGTT GTGTTTTACA GTATTATGTA GTCTGTTTTT TATGCAAAAT
  241 CTAATTTAAT ATATTGATAT TTATATCATT TTACGTTTCT CGTTCAGCTT TTTTGTACAA
  301 ACTTGTTGAT ATCCGGATCC AGGCCTGTCG ACGCTAGCGA ACGTCTTTCC AGGGTTTCTA
  361 CGAGGTTTTT GGTGTTTGGT TTGTAGTGAA GTGCGGTGTA CACTTAATCA AAACAATTCC
  421 TTAATAAAGA AGTCTAACGA TATTTATTGA TGATACGGAA GGAAGTATGC TAAACATGTA
  481 AATAGACATT AAAATAATGC ATTGCTTGCA AAAAGAAGCT TACTCAGTCA GGTGTTTTAA
  541 GCTTTCTAAT CATAAAAAGA TTAGGATCTT CATCAATTTC ACCTTTTATT TTCTCAATTT
601 GTAAGTGAAA GATTGGATTG GTAAATTTCC ATTAAAACTC AACATGTG
//
Primer: | Sequence: RDB12325_A3Klb.seq
>RDB12325_ADH_rev_D12_12.ab1
    1 TGGGAGTCTC AAGAATAAGA ATTTTCGTTT TAAACCTAAG AGTCACTTTA AAATTTGTAT
   61 ACACTTATTT TTTTTATAAC TTATTTAATA ATAAAAATCA TAAATCATAA GAAATTCGCT
  121 TATTTAGAAG TGGCGCGCCG GATCCTCTAG AGTCGAGATC TCAGTGGTGA TGGTGGTGAT
  181 GTTTGTCATC GTCGTCCTTG TAGTCCATGG GTTTGTATAG TTCATCCATG CCATGTGTAA
  241 TCCCAGCAGC TGTTACAAAC TCAAGAAGGA CCATGTGGTC TCTCTTTTCG TTGGGATCTT
  301 TCGAAAGGGC AGATTGTGTG GACAGGTAAT GGTTGTCTGG TAAAAGGACA GGGCCATCGC
  361 CAATTGGAGT ATTTTGTTGA TAATGGTCTG CTAGTTGAAC GCTTCCATCT TCAATGTTGT
  421 GTCTAATTTT GAAGTTAACT TTGATTCCAT TCTTTTGTTT GTCTGCCATG ATGTATACAT
  481 TGTGTGAGTT ATAGTTGTAT TCCAATTTGT GTCCAAGAAT GTTTCCATCT TCTTTAAAAT
  541 CAATACCTTT TAACTCGATT CTATTAACAA GGGTATCACC TTCAAACTTG ACTTCAGCAC
  601 GTGTCTTGTA GTTCCCGTCA TCTTTGAAAA ATATAGTTCT TTCCTGTACA TAGCCTTCGG
661 GCATGGCACT CTTG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles


2019.09.27

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