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pDUAL-FFH31c

S. pombe 3 ter end FLAG2-His6-tagged vector with cam1 promoter

Catalog number RDB06490
Resource name pDUAL-FFH31c
Clone info. S. pombe 3 ter end FLAG2-His6-tagged vector with cam1 promoter. Multicopy marker, ura4+; Integration marker, leu1+.
Gateway compatible.
Vector backbone pDUAL (Plasmid)
Selectable markers E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Reference of nucleotide sequence AB364144 (DDBJ accession number)
Depositor Yoshida, Minoru | Matsuyama, Akihisa |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., A., Yeast, 25 (5): 371-376 (2008)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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Material Transfer Agreement (MTA) [Word]
Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.
Distribution information (domestic users) [open/close]


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RDB06490 pDUAL-FFH31c DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB06490z.seq from Depositor

Original reference

original Matsuyama, A., A series of promoters for constitutive expression of heterologous genes in fission yeast. Yeast, 25 (5):371-376 (2008). PMID 18437702.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12778_A4Dp.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: ADHrev | Sequence: RDB12778_A4Dpa.seq
>06490_12778_A4Dp_ADHrev_F08_17.ab1
    1 GCGGGCCAAG TAAAAATTTC TCAAGAAAAA AGAATTTTCG TTTTAAAACC TAAGACCCCA
   61 CTTTAAAATT TGTATACACT TATTTTTTTT ATAACTTATT TAATAATAAA AATCATAAAT
  121 CATAAGAAAT TCGCTTATTT AGAAGTGGCG CGCCGGATCC TCTAGAGTCG AGATCTCAGT
  181 GGTGATGGTG GTGATGTTTG TCATCGTCGT CCTTGTAGTC CATGGGTTTA TCGTCATCAT
  241 CCTTATAATC CATGATATCA ACCACTTTGT ACAAGAAAGC TGAACGAGAA ACGTAAAATG
  301 ATATAAATAT CAATATATTA AATTAGATTT TGCATAAAAA ACAGACTACA TAATACTGTA
  361 AAACACAACA TATCCAGTCA CTATGGTCGA CCTGCAGACT GGCTGTGTAT AAGGGAGCCT
  421 GACATTTATA TTCCCCAGAA CATCAGGTTA ATGGCGTTTT TGATGTCATT TTCGCGGTGG
  481 CTGAGATCAG CCACTTCTTC CCCGATAACG GAGACCGGCA CACTGGCCAT ATCGGTGGTC
  541 ATCATGCGCC AGCTTTCATC CCCGATATGC ACCACCGGGT AAAGTTCACG GGAGACTTTA
  601 TCTGACAGCA GACGTGCACT GGCCAGGGGG ATCACCATCC GTCGCCCGGG CGTGTCAATA
  661 ATATCACTCT GTACATCCAC AAACAGACGA TAACGGCTCT CTCTTTTATA GGTGTAAACC
  721 TTAAACTGCA TTTCACCCAG CCCCTGTTCT CGTCAGCAAA AGAGCCGTTC ATTTCAAATA
  781 AAACCGGGCG ACCTCAGCCA TCCCTTCCTG ATTTTCCGCT TTCCCAGCGT TCCGGCACGC
  841 AGACCACGGG CTTCATTCTG CATGGTTGTG CTTACCCAGA CCGGAGATAA TTGACATCAT
  901 ATATGCCTTG AGCAAACTGA TAGCTGTCGC TGTCAACTGT CCCTGTAATA CGCCTGCTTC
  961 ATAACATACC TCTTTTTGAC ATACTTCGGG GTATACATAT CAGTATAATA TTCTTATACC
 1021 GCAAAAAATC AGCGCGCAAA TACCGCATAC TGCTATCTGG CCTTTTAGTA AGCCCGATTC
 1081 CAGAATTCTT ACGCCCCCGC CCTGGCCACA CTCAATCCGC CGATACTGGT TTGTAAATTC
 1141 AATTAAGGCA ATTCAGCTCG ACACATGGAG GCCATCCACA CCCGCCAGGT GAATGAAACC
 1201 CTGAAAATCT GCCTCAAGGC GGCGACTATC TCCCAGAGCA CACCACTCTG TGCTGTCTGC
//
Primer: Cm_N_rev | Sequence: RDB12778_A4Dpb.seq
>06490_12778_A4Dp_Cm_N_rev_G08_20.ab1
    1 CGTAAACCCC TAAATGTTTC TTTACGATGC CATTGGGATA TATCAACGCG TGGTATATCC
   61 AGTGATTTTT TTCTCCATTT TATCTTCCTT AGCTCCTGAA AATCTCGACG GATCCTAACT
  121 CAAAATCCAC ACATTATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGC
  181 GGCCGCCATA GTGACTGGAT ATGTTGTGTT TTACAGTATT ATGTAGTCTG TTTTTTATGC
  241 AAAATCTAAT TTAATATATT GATATTTATA TCATTTTACG TTTCTCGTTC AGCTTTTTTG
  301 TACAAACTTG TTGATATCCG GATCCAGGCC TGTCGACGCT AGCATCAGTA ACTCTAAAGT
  361 CCTTCTATTC CTTTTTTTAA CCTTTATTTT TGGCGGTTAG TGTTTTGCTC TAGTTACGTT
  421 AAAGTGCCTT GGAGAAATGA GGACTACCGT TGTCGCTTCC TAATGTTGCA AGTCCTACCA
  481 TCAACAGTGA TGTCAACAAA CAATGAGAGT TTATTACATT ATCGTATATG CTCTAACAAA
  541 CCAGTAACGT ACTTGCTATA AACCGCACTA AAACCACTAA ACGGCGGATG AAGGAACTTT
  601 TTCAATGTCA GCTACGCATT TATTGGTATC TATTTAACGA CACCTAGAAC TGCCGTGTGG
  661 TTTACTTGTG ACCTATTGTA TTATGGTACA CAGGTAAATA CATTTAGTAA CTTAGAATGA
  721 TATTCTGATA TAGAGGTTAT ATTGTTACCT ATCACCGAAT TAGCATAAAA GTGAATAGAG
  781 AAACGAGGTT TTGTTTATTT CAATTCATAA AATTTAAGCA TTCTATTATT TTTTTTGTTA
  841 TTCATTTTAT AACTCTTAGT AATCTACCTA AGTAACTTAT CATGTACAGC TTGACAGAGC
  901 AAATCAAACA TGTGATAATT AGTGATACAC AACGTAGTAT GCTATAGCGC ATGCAAGCTT
  961 GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA
 1021 CAACATACGA GCCGGAAGCA TAAAGTTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC
 1081 TCACATTTAT TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CCGGGGAAAC CTGTCGTGCC
 1141 AGCTGCATTA ATGAATTCGG CCACGCGCGG GGAGAAGCCG TTGCGTATTG GGCGCTCTCG
1201 GCTCTCGCCT ACATGAACTG CAGTCGCCTT CGGATG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Matsuyama, A., A series of promoters for constitutive expression of heterologous genes in fission yeast. Yeast, 25 (5):371-376 (2008). PMID 18437702.

2019.09.27

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