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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pDUAL-FFH31c (#RDB06490)

S. pombe 3 ter end FLAG2-His6-tagged vector with cam1 promoter

Clone info. S. pombe 3 ter end FLAG2-His6-tagged vector with cam1 promoter. Multicopy marker, ura4+; Integration marker, leu1+.
Comment Gateway compatible.
Vector backbone pDUAL (RDB06178) (Plasmid)
Selectable markers E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Reference of insert sequence AB364144 (DDBJ accession number)
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
 
Sequence RDB06490z.seq

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., A., Yeast, 25 (5): 371-376 (2008)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast, 25 (5): 371-376 (2008)) の引用と、寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
備考 このクローンの増殖にはSurvival2 (Invitrogen) あるいは同等の宿主を使用してください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06490 pDUAL-FFH31c DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pDUAL-FFH31c was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06490).

Reference section:

Matsuyama, A., Shirai, A., Yoshida, M., A series of promoters for constitutive expression of heterologous genes in fission yeast. Yeast, 25 (5):371-376 (2008). PMID 18437702. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12778_A4Dp.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: ADHrev
Sequence file: RDB12778_A4Dpa.seq check
>06490_12778_A4Dp_ADHrev_F08_17.ab1
    1 GCGGGCCAAG TAAAAATTTC TCAAGAAAAA AGAATTTTCG TTTTAAAACC TAAGACCCCA
   61 CTTTAAAATT TGTATACACT TATTTTTTTT ATAACTTATT TAATAATAAA AATCATAAAT
  121 CATAAGAAAT TCGCTTATTT AGAAGTGGCG CGCCGGATCC TCTAGAGTCG AGATCTCAGT
  181 GGTGATGGTG GTGATGTTTG TCATCGTCGT CCTTGTAGTC CATGGGTTTA TCGTCATCAT
  241 CCTTATAATC CATGATATCA ACCACTTTGT ACAAGAAAGC TGAACGAGAA ACGTAAAATG
  301 ATATAAATAT CAATATATTA AATTAGATTT TGCATAAAAA ACAGACTACA TAATACTGTA
  361 AAACACAACA TATCCAGTCA CTATGGTCGA CCTGCAGACT GGCTGTGTAT AAGGGAGCCT
  421 GACATTTATA TTCCCCAGAA CATCAGGTTA ATGGCGTTTT TGATGTCATT TTCGCGGTGG
  481 CTGAGATCAG CCACTTCTTC CCCGATAACG GAGACCGGCA CACTGGCCAT ATCGGTGGTC
  541 ATCATGCGCC AGCTTTCATC CCCGATATGC ACCACCGGGT AAAGTTCACG GGAGACTTTA
  601 TCTGACAGCA GACGTGCACT GGCCAGGGGG ATCACCATCC GTCGCCCGGG CGTGTCAATA
  661 ATATCACTCT GTACATCCAC AAACAGACGA TAACGGCTCT CTCTTTTATA GGTGTAAACC
  721 TTAAACTGCA TTTCACCCAG CCCCTGTTCT CGTCAGCAAA AGAGCCGTTC ATTTCAAATA
  781 AAACCGGGCG ACCTCAGCCA TCCCTTCCTG ATTTTCCGCT TTCCCAGCGT TCCGGCACGC
  841 AGACCACGGG CTTCATTCTG CATGGTTGTG CTTACCCAGA CCGGAGATAA TTGACATCAT
  901 ATATGCCTTG AGCAAACTGA TAGCTGTCGC TGTCAACTGT CCCTGTAATA CGCCTGCTTC
  961 ATAACATACC TCTTTTTGAC ATACTTCGGG GTATACATAT CAGTATAATA TTCTTATACC
 1021 GCAAAAAATC AGCGCGCAAA TACCGCATAC TGCTATCTGG CCTTTTAGTA AGCCCGATTC
 1081 CAGAATTCTT ACGCCCCCGC CCTGGCCACA CTCAATCCGC CGATACTGGT TTGTAAATTC
 1141 AATTAAGGCA ATTCAGCTCG ACACATGGAG GCCATCCACA CCCGCCAGGT GAATGAAACC
 1201 CTGAAAATCT GCCTCAAGGC GGCGACTATC TCCCAGAGCA CACCACTCTG TGCTGTCTGC
//
Primer: Cm_N_rev
Sequence file: RDB12778_A4Dpb.seq check
>06490_12778_A4Dp_Cm_N_rev_G08_20.ab1
    1 CGTAAACCCC TAAATGTTTC TTTACGATGC CATTGGGATA TATCAACGCG TGGTATATCC
   61 AGTGATTTTT TTCTCCATTT TATCTTCCTT AGCTCCTGAA AATCTCGACG GATCCTAACT
  121 CAAAATCCAC ACATTATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGC
  181 GGCCGCCATA GTGACTGGAT ATGTTGTGTT TTACAGTATT ATGTAGTCTG TTTTTTATGC
  241 AAAATCTAAT TTAATATATT GATATTTATA TCATTTTACG TTTCTCGTTC AGCTTTTTTG
  301 TACAAACTTG TTGATATCCG GATCCAGGCC TGTCGACGCT AGCATCAGTA ACTCTAAAGT
  361 CCTTCTATTC CTTTTTTTAA CCTTTATTTT TGGCGGTTAG TGTTTTGCTC TAGTTACGTT
  421 AAAGTGCCTT GGAGAAATGA GGACTACCGT TGTCGCTTCC TAATGTTGCA AGTCCTACCA
  481 TCAACAGTGA TGTCAACAAA CAATGAGAGT TTATTACATT ATCGTATATG CTCTAACAAA
  541 CCAGTAACGT ACTTGCTATA AACCGCACTA AAACCACTAA ACGGCGGATG AAGGAACTTT
  601 TTCAATGTCA GCTACGCATT TATTGGTATC TATTTAACGA CACCTAGAAC TGCCGTGTGG
  661 TTTACTTGTG ACCTATTGTA TTATGGTACA CAGGTAAATA CATTTAGTAA CTTAGAATGA
  721 TATTCTGATA TAGAGGTTAT ATTGTTACCT ATCACCGAAT TAGCATAAAA GTGAATAGAG
  781 AAACGAGGTT TTGTTTATTT CAATTCATAA AATTTAAGCA TTCTATTATT TTTTTTGTTA
  841 TTCATTTTAT AACTCTTAGT AATCTACCTA AGTAACTTAT CATGTACAGC TTGACAGAGC
  901 AAATCAAACA TGTGATAATT AGTGATACAC AACGTAGTAT GCTATAGCGC ATGCAAGCTT
  961 GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA
 1021 CAACATACGA GCCGGAAGCA TAAAGTTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC
 1081 TCACATTTAT TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CCGGGGAAAC CTGTCGTGCC
 1141 AGCTGCATTA ATGAATTCGG CCACGCGCGG GGAGAAGCCG TTGCGTATTG GGCGCTCTCG
1201 GCTCTCGCCT ACATGAACTG CAGTCGCCTT CGGATG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Matsuyama, A., A series of promoters for constitutive expression of heterologous genes in fission yeast. Yeast, 25 (5):371-376 (2008). PMID 18437702. [link to RRC of NBRP]