S. pombe 3 ter end FLAG2-His6-tagged vector with cam1 promoter
Clone info. | S. pombe 3 ter end FLAG2-His6-tagged vector with cam1 promoter. Multicopy marker, ura4+; Integration marker, leu1+. |
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Comment | Gateway compatible. |
Vector backbone | pDUAL (RDB06178) (Plasmid) |
Selectable markers | E. coli: Amp^r, Cm^r, ccdB. |
Growth remarks | Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine. |
Reference of insert sequence | AB364144 (DDBJ accession number) |
Depositor|Developer | Yoshida, Minoru | Matsuyama, Akihisa | |
Sequence | RDB06490z.seq |
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Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., A., Yeast, 25 (5): 371-376 (2008)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation. |
Remarks | Use Survival2 (Invitrogen) or equivalent to amplify this clone. |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast, 25 (5): 371-376 (2008)) の引用と、寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。 |
備考 | このクローンの増殖にはSurvival2 (Invitrogen) あるいは同等の宿主を使用してください。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB06490 | pDUAL-FFH31c | DNA solution |
Materials & Methods section:
The pDUAL-FFH31c was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06490). |
Reference section:
Matsuyama, A., Shirai, A., Yoshida, M., A series of promoters for constitutive expression of heterologous genes in fission yeast. Yeast, 25 (5):371-376 (2008). PMID 18437702. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB12778_A4Dp.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: ADHrev Sequence file: RDB12778_A4Dpa.seq ![]() |
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>06490_12778_A4Dp_ADHrev_F08_17.ab1 1 GCGGGCCAAG TAAAAATTTC TCAAGAAAAA AGAATTTTCG TTTTAAAACC TAAGACCCCA 61 CTTTAAAATT TGTATACACT TATTTTTTTT ATAACTTATT TAATAATAAA AATCATAAAT 121 CATAAGAAAT TCGCTTATTT AGAAGTGGCG CGCCGGATCC TCTAGAGTCG AGATCTCAGT 181 GGTGATGGTG GTGATGTTTG TCATCGTCGT CCTTGTAGTC CATGGGTTTA TCGTCATCAT 241 CCTTATAATC CATGATATCA ACCACTTTGT ACAAGAAAGC TGAACGAGAA ACGTAAAATG 301 ATATAAATAT CAATATATTA AATTAGATTT TGCATAAAAA ACAGACTACA TAATACTGTA 361 AAACACAACA TATCCAGTCA CTATGGTCGA CCTGCAGACT GGCTGTGTAT AAGGGAGCCT 421 GACATTTATA TTCCCCAGAA CATCAGGTTA ATGGCGTTTT TGATGTCATT TTCGCGGTGG 481 CTGAGATCAG CCACTTCTTC CCCGATAACG GAGACCGGCA CACTGGCCAT ATCGGTGGTC 541 ATCATGCGCC AGCTTTCATC CCCGATATGC ACCACCGGGT AAAGTTCACG GGAGACTTTA 601 TCTGACAGCA GACGTGCACT GGCCAGGGGG ATCACCATCC GTCGCCCGGG CGTGTCAATA 661 ATATCACTCT GTACATCCAC AAACAGACGA TAACGGCTCT CTCTTTTATA GGTGTAAACC 721 TTAAACTGCA TTTCACCCAG CCCCTGTTCT CGTCAGCAAA AGAGCCGTTC ATTTCAAATA 781 AAACCGGGCG ACCTCAGCCA TCCCTTCCTG ATTTTCCGCT TTCCCAGCGT TCCGGCACGC 841 AGACCACGGG CTTCATTCTG CATGGTTGTG CTTACCCAGA CCGGAGATAA TTGACATCAT 901 ATATGCCTTG AGCAAACTGA TAGCTGTCGC TGTCAACTGT CCCTGTAATA CGCCTGCTTC 961 ATAACATACC TCTTTTTGAC ATACTTCGGG GTATACATAT CAGTATAATA TTCTTATACC 1021 GCAAAAAATC AGCGCGCAAA TACCGCATAC TGCTATCTGG CCTTTTAGTA AGCCCGATTC 1081 CAGAATTCTT ACGCCCCCGC CCTGGCCACA CTCAATCCGC CGATACTGGT TTGTAAATTC 1141 AATTAAGGCA ATTCAGCTCG ACACATGGAG GCCATCCACA CCCGCCAGGT GAATGAAACC 1201 CTGAAAATCT GCCTCAAGGC GGCGACTATC TCCCAGAGCA CACCACTCTG TGCTGTCTGC // |
Primer: Cm_N_rev Sequence file: RDB12778_A4Dpb.seq ![]() |
>06490_12778_A4Dp_Cm_N_rev_G08_20.ab1 1 CGTAAACCCC TAAATGTTTC TTTACGATGC CATTGGGATA TATCAACGCG TGGTATATCC 61 AGTGATTTTT TTCTCCATTT TATCTTCCTT AGCTCCTGAA AATCTCGACG GATCCTAACT 121 CAAAATCCAC ACATTATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGC 181 GGCCGCCATA GTGACTGGAT ATGTTGTGTT TTACAGTATT ATGTAGTCTG TTTTTTATGC 241 AAAATCTAAT TTAATATATT GATATTTATA TCATTTTACG TTTCTCGTTC AGCTTTTTTG 301 TACAAACTTG TTGATATCCG GATCCAGGCC TGTCGACGCT AGCATCAGTA ACTCTAAAGT 361 CCTTCTATTC CTTTTTTTAA CCTTTATTTT TGGCGGTTAG TGTTTTGCTC TAGTTACGTT 421 AAAGTGCCTT GGAGAAATGA GGACTACCGT TGTCGCTTCC TAATGTTGCA AGTCCTACCA 481 TCAACAGTGA TGTCAACAAA CAATGAGAGT TTATTACATT ATCGTATATG CTCTAACAAA 541 CCAGTAACGT ACTTGCTATA AACCGCACTA AAACCACTAA ACGGCGGATG AAGGAACTTT 601 TTCAATGTCA GCTACGCATT TATTGGTATC TATTTAACGA CACCTAGAAC TGCCGTGTGG 661 TTTACTTGTG ACCTATTGTA TTATGGTACA CAGGTAAATA CATTTAGTAA CTTAGAATGA 721 TATTCTGATA TAGAGGTTAT ATTGTTACCT ATCACCGAAT TAGCATAAAA GTGAATAGAG 781 AAACGAGGTT TTGTTTATTT CAATTCATAA AATTTAAGCA TTCTATTATT TTTTTTGTTA 841 TTCATTTTAT AACTCTTAGT AATCTACCTA AGTAACTTAT CATGTACAGC TTGACAGAGC 901 AAATCAAACA TGTGATAATT AGTGATACAC AACGTAGTAT GCTATAGCGC ATGCAAGCTT 961 GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA 1021 CAACATACGA GCCGGAAGCA TAAAGTTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC 1081 TCACATTTAT TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CCGGGGAAAC CTGTCGTGCC 1141 AGCTGCATTA ATGAATTCGG CCACGCGCGG GGAGAAGCCG TTGCGTATTG GGCGCTCTCG 1201 GCTCTCGCCT ACATGAACTG CAGTCGCCTT CGGATG // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Matsuyama, A., A series of promoters for constitutive expression of heterologous genes in fission yeast. Yeast, 25 (5):371-376 (2008). PMID 18437702. [link to RRC of NBRP] |
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