Resource data sheet
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pLYS1U-HFY21c

S. pombe 5 ter end His6-FLAG-YFP-tagged vector with tub1 promoter

Catalog number RDB06475
Resource name pLYS1U-HFY21c
Clone info. S. pombe 5 ter end His6-FLAG-YFP-tagged vector with tub1 promoter.
Gateway compatible.
Vector backbone pUC119 (Plasmid)
Selectable markers E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor Yoshida, Minoru | Matsuyama, Akihisa |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature(s). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.
Please use Schedule A (GFP-A) additionally.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06475 pLYS1U-HFY21c DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB06475z.seq from Depositor

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12323_A3Kl.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: | Sequence: RDB12323_A3Kla.seq
>RDB12323_EGFP_C_D11_11.ab1
    1 CGGGGGAATC TCGGCATGCA CGAGCTGTAC AAGGATATCA CAAGTTTGTA CAAAAAAGCT
   61 GAACGAGAAA CGTAAAATGA TATAAATATC AATATATTAA ATTAGATTTT GCATAAAAAA
  121 CAGACTACAT AATACTGTAA AACACAACAT ATCCAGTCAC TATGGCGGCC GCATTAGGCA
  181 CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATAATGT GTGGATTTTG AGTTAGGATC
  241 CGTCGAGATT TTCAGGAGCT AAGGAAGCTA AAATGGAGAA AAAAATCACT GGATATACCA
  301 CCGTTGATAT ATCCCAATGG CATCGTAAAG AACATTTTGA GGCATTTCAG TCAGTTGCTC
  361 AATGTACCTA TAACCAGACC GTTCAGCTGG ATATTACGGC CTTTTTAAAG ACCGTAAAGA
  421 AAAATAAGCA CAAGTTTTAT CCGGCCTTTA TTCACATTCT TGCCCGCCTG ATGAATGCTC
  481 ATCCGGAATT CCGTATGGCA ATGAAAGACG GTGAGCTGGT GATATGGGAT AGTGTTCACC
  541 CTTGTTACAC CGTTTTCCAT GAGCAAACTG AAACGTTTTC ATCGCTCTGG AGTGAATACC
  601 ACGACGATTT CCGGCAGTTT CTACACATAT ATTCGCAAGA TGTGGCGTGT TACGGTGAAA
661 ACCTGGCCTA TTTCCC
//
Primer: | Sequence: RDB12323_A3Klb.seq
>RDB12323_ADH_rev_E11_14.ab1
    1 TGAGGAAACT CAAGGAATAA GAATTTTCGT TTTAAAACCT AAGAGTCACT TTAAAATTTG
   61 TATACACTTA TTTTTTTTAT AACTTATTTA ATAATAAAAA TCATAAATCA TAAGAAATTC
  121 GCTTATTTAG AAGTGGCGCG CCGGATCCTC TAGAGTCGAG ATCTCATGAT ATCACCACTT
  181 TGTACAAGAA AGCTGAACGA GAAACGTAAA ATGATATAAA TATCAATATA TTAAATTAGA
  241 TTTTGCATAA AAAACAGACT ACATAATACT GTAAAACACA ACATATCCAG TCACTATGGT
  301 CGACCTGCAG ACTGGCTGTG TATAAGGGAG CCTGACATTT ATATTCCCCA GAACATCAGG
  361 TTAATGGCGT TTTTGATGTC ATTTTCGCGG TGGCTGAGAT CAGCCACTTC TTCCCCGATA
  421 ACGGAGACCG GCACACTGGC CATATCGGTG GTCATCATGC GCCAGCTTTC ATCCCCGATA
  481 TGCACCACCG GGTAAAGTTC ACGGGAGACT TTATCTGACA GCAGACGTGC ACTGGCCAGG
  541 GGGATCACCA TCCGTCGCCC GGGCGTGTCA ATAATATCAC TCTGTACATC CACAAACAGA
  601 CGATAACGGC TCTCTCTTTT ATAGGTGTAA ACCTTAAACT GCATTTCACC AGCCCCTGTT
661 CTCGTCAGCA AAAGAGC
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

user report Ito, D., Pericentrin-mediated SAS-6 recruitment promotes centriole assembly. Elife 8. pii: e41418 (2019). PMID 31182187.

2019.10.05

GNP_filter3_RDBDEP_html_190927.pl