Please review the QC test results indicated by icon below as well as clone information before placing your order.

pLYS1U-HFY21c

S. pombe 5 ter end His6-FLAG-YFP-tagged vector with tub1 promoter

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature(s). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
Remarks	Use Survival2 (Invitrogen) or equivalent to amplify this clone.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献の引用と、寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします （Schedule AとSchedule Bを入手[link])。
備考	このクローンの増殖にはSurvival2 (Invitrogen) あるいは同等の宿主を使用してください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB12323_A3Kl.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Sequence file: RDB12323_A3Kla.seq
>RDB12323_EGFP_C_D11_11.ab1     1 CGGGGGAATC TCGGCATGCA CGAGCTGTAC AAGGATATCA CAAGTTTGTA CAAAAAAGCT    61 GAACGAGAAA CGTAAAATGA TATAAATATC AATATATTAA ATTAGATTTT GCATAAAAAA   121 CAGACTACAT AATACTGTAA AACACAACAT ATCCAGTCAC TATGGCGGCC GCATTAGGCA   181 CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATAATGT GTGGATTTTG AGTTAGGATC   241 CGTCGAGATT TTCAGGAGCT AAGGAAGCTA AAATGGAGAA AAAAATCACT GGATATACCA   301 CCGTTGATAT ATCCCAATGG CATCGTAAAG AACATTTTGA GGCATTTCAG TCAGTTGCTC   361 AATGTACCTA TAACCAGACC GTTCAGCTGG ATATTACGGC CTTTTTAAAG ACCGTAAAGA   421 AAAATAAGCA CAAGTTTTAT CCGGCCTTTA TTCACATTCT TGCCCGCCTG ATGAATGCTC   481 ATCCGGAATT CCGTATGGCA ATGAAAGACG GTGAGCTGGT GATATGGGAT AGTGTTCACC   541 CTTGTTACAC CGTTTTCCAT GAGCAAACTG AAACGTTTTC ATCGCTCTGG AGTGAATACC   601 ACGACGATTT CCGGCAGTTT CTACACATAT ATTCGCAAGA TGTGGCGTGT TACGGTGAAA 661 ACCTGGCCTA TTTCCC //
Primer: Sequence file: RDB12323_A3Klb.seq
>RDB12323_ADH_rev_E11_14.ab1     1 TGAGGAAACT CAAGGAATAA GAATTTTCGT TTTAAAACCT AAGAGTCACT TTAAAATTTG    61 TATACACTTA TTTTTTTTAT AACTTATTTA ATAATAAAAA TCATAAATCA TAAGAAATTC   121 GCTTATTTAG AAGTGGCGCG CCGGATCCTC TAGAGTCGAG ATCTCATGAT ATCACCACTT   181 TGTACAAGAA AGCTGAACGA GAAACGTAAA ATGATATAAA TATCAATATA TTAAATTAGA   241 TTTTGCATAA AAAACAGACT ACATAATACT GTAAAACACA ACATATCCAG TCACTATGGT   301 CGACCTGCAG ACTGGCTGTG TATAAGGGAG CCTGACATTT ATATTCCCCA GAACATCAGG   361 TTAATGGCGT TTTTGATGTC ATTTTCGCGG TGGCTGAGAT CAGCCACTTC TTCCCCGATA   421 ACGGAGACCG GCACACTGGC CATATCGGTG GTCATCATGC GCCAGCTTTC ATCCCCGATA   481 TGCACCACCG GGTAAAGTTC ACGGGAGACT TTATCTGACA GCAGACGTGC ACTGGCCAGG   541 GGGATCACCA TCCGTCGCCC GGGCGTGTCA ATAATATCAC TCTGTACATC CACAAACAGA   601 CGATAACGGC TCTCTCTTTT ATAGGTGTAA ACCTTAAACT GCATTTCACC AGCCCCTGTT 661 CTCGTCAGCA AAAGAGC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.07.13

GNP_filter3_RDBDEP_html_220713.pl