RDB06475 - pLYS1U-HFY21c

Resource data sheet
Prev.

pLYS1U-HFY21c

S. pombe 5 ter end His6-FLAG-YFP-tagged vector with tub1 promoter

Catalog number	RDB06475
Resource name	pLYS1U-HFY21c
Clone info.	S. pombe 5 ter end His6-FLAG-YFP-tagged vector with tub1 promoter. Gateway compatible.
Vector backbone	pUC119 (Plasmid)
Selectable markers	E. coli: Amp^r, Cm^r, ccdB.
Growth remarks	Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor	Yoshida, Minoru \| Matsuyama, Akihisa \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature(s). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.

Ordering Please visit Information of Request for Distribution.
Order form [Credit Card Payment] [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]

Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.
Please use Schedule A (GFP-A) additionally.

Distribution information (domestic users) [open/close]

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature(s). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
Ordering	Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Schedule A (GFP-A) [Link]
Remarks	Use Survival2 (Invitrogen) or equivalent to amplify this clone. Please use Schedule A (GFP-A) additionally.

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献の引用と、寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
提供依頼	手続きの詳細は、「提供申込みについて」をご覧ください。提供依頼書 [Word] 提供同意書 [Word] Schedule A（GFP-A, 英語版のみ） [Link]
備考	このクローンの増殖にはSurvival2 (Invitrogen) あるいは同等の宿主を使用してください。 Schedule A（GFP-A, 英語版のみ）が追加で必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB06475	pLYS1U-HFY21c	DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file	Sequence RDB06475z.seq from Depositor

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content	Expression Vector Backbone of Schizosaccharomyces pombe (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet	RDB12323_A3Kl.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: \| Sequence: RDB12323_A3Kla.seq
>RDB12323_EGFP_C_D11_11.ab1 1 CGGGGGAATC TCGGCATGCA CGAGCTGTAC AAGGATATCA CAAGTTTGTA CAAAAAAGCT 61 GAACGAGAAA CGTAAAATGA TATAAATATC AATATATTAA ATTAGATTTT GCATAAAAAA 121 CAGACTACAT AATACTGTAA AACACAACAT ATCCAGTCAC TATGGCGGCC GCATTAGGCA 181 CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATAATGT GTGGATTTTG AGTTAGGATC 241 CGTCGAGATT TTCAGGAGCT AAGGAAGCTA AAATGGAGAA AAAAATCACT GGATATACCA 301 CCGTTGATAT ATCCCAATGG CATCGTAAAG AACATTTTGA GGCATTTCAG TCAGTTGCTC 361 AATGTACCTA TAACCAGACC GTTCAGCTGG ATATTACGGC CTTTTTAAAG ACCGTAAAGA 421 AAAATAAGCA CAAGTTTTAT CCGGCCTTTA TTCACATTCT TGCCCGCCTG ATGAATGCTC 481 ATCCGGAATT CCGTATGGCA ATGAAAGACG GTGAGCTGGT GATATGGGAT AGTGTTCACC 541 CTTGTTACAC CGTTTTCCAT GAGCAAACTG AAACGTTTTC ATCGCTCTGG AGTGAATACC 601 ACGACGATTT CCGGCAGTTT CTACACATAT ATTCGCAAGA TGTGGCGTGT TACGGTGAAA 661 ACCTGGCCTA TTTCCC //
Primer: \| Sequence: RDB12323_A3Klb.seq
>RDB12323_ADH_rev_E11_14.ab1 1 TGAGGAAACT CAAGGAATAA GAATTTTCGT TTTAAAACCT AAGAGTCACT TTAAAATTTG 61 TATACACTTA TTTTTTTTAT AACTTATTTA ATAATAAAAA TCATAAATCA TAAGAAATTC 121 GCTTATTTAG AAGTGGCGCG CCGGATCCTC TAGAGTCGAG ATCTCATGAT ATCACCACTT 181 TGTACAAGAA AGCTGAACGA GAAACGTAAA ATGATATAAA TATCAATATA TTAAATTAGA 241 TTTTGCATAA AAAACAGACT ACATAATACT GTAAAACACA ACATATCCAG TCACTATGGT 301 CGACCTGCAG ACTGGCTGTG TATAAGGGAG CCTGACATTT ATATTCCCCA GAACATCAGG 361 TTAATGGCGT TTTTGATGTC ATTTTCGCGG TGGCTGAGAT CAGCCACTTC TTCCCCGATA 421 ACGGAGACCG GCACACTGGC CATATCGGTG GTCATCATGC GCCAGCTTTC ATCCCCGATA 481 TGCACCACCG GGTAAAGTTC ACGGGAGACT TTATCTGACA GCAGACGTGC ACTGGCCAGG 541 GGGATCACCA TCCGTCGCCC GGGCGTGTCA ATAATATCAC TCTGTACATC CACAAACAGA 601 CGATAACGGC TCTCTCTTTT ATAGGTGTAA ACCTTAAACT GCATTTCACC AGCCCCTGTT 661 CTCGTCAGCA AAAGAGC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

user report	Ito, D., Pericentrin-mediated SAS-6 recruitment promotes centriole assembly. Elife 8. pii: e41418 (2019). PMID 31182187.

2019.10.05

GNP_filter3_RDBDEP_html_190927.pl