S. pombe 5 ter end His6-FLAG-YFP-tagged vector with tub1 promoter
Catalog number | RDB06475 |
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Resource name | pLYS1U-HFY21c |
Clone info. | S. pombe 5 ter end His6-FLAG-YFP-tagged vector with tub1 promoter. Gateway compatible. |
Vector backbone | pUC119 (Plasmid) |
Selectable markers | E. coli: Amp^r, Cm^r, ccdB. |
Growth remarks | Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine. |
Depositor | Yoshida, Minoru | Matsuyama, Akihisa | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature(s). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation. |
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Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Schedule A (GFP-A) [Link] |
Remarks | Use Survival2 (Invitrogen) or equivalent to amplify this clone. Please use Schedule A (GFP-A) additionally. |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB06475 | pLYS1U-HFY21c | DNA solution |
Electronic file
Electronic file | Sequence RDB06475z.seq from Depositor |
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Original reference
Further references such as user reports and related articles (go to bottom)
Featured content
Featured content | Expression Vector Backbone of Schizosaccharomyces pombe (English text) |
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RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
Test sheet | RDB12323_A3Kl.pdf |
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Nucleotide sequence of a portion of this resource (if available).
Primer: | Sequence: RDB12323_A3Kla.seq |
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>RDB12323_EGFP_C_D11_11.ab1 1 CGGGGGAATC TCGGCATGCA CGAGCTGTAC AAGGATATCA CAAGTTTGTA CAAAAAAGCT 61 GAACGAGAAA CGTAAAATGA TATAAATATC AATATATTAA ATTAGATTTT GCATAAAAAA 121 CAGACTACAT AATACTGTAA AACACAACAT ATCCAGTCAC TATGGCGGCC GCATTAGGCA 181 CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATAATGT GTGGATTTTG AGTTAGGATC 241 CGTCGAGATT TTCAGGAGCT AAGGAAGCTA AAATGGAGAA AAAAATCACT GGATATACCA 301 CCGTTGATAT ATCCCAATGG CATCGTAAAG AACATTTTGA GGCATTTCAG TCAGTTGCTC 361 AATGTACCTA TAACCAGACC GTTCAGCTGG ATATTACGGC CTTTTTAAAG ACCGTAAAGA 421 AAAATAAGCA CAAGTTTTAT CCGGCCTTTA TTCACATTCT TGCCCGCCTG ATGAATGCTC 481 ATCCGGAATT CCGTATGGCA ATGAAAGACG GTGAGCTGGT GATATGGGAT AGTGTTCACC 541 CTTGTTACAC CGTTTTCCAT GAGCAAACTG AAACGTTTTC ATCGCTCTGG AGTGAATACC 601 ACGACGATTT CCGGCAGTTT CTACACATAT ATTCGCAAGA TGTGGCGTGT TACGGTGAAA 661 ACCTGGCCTA TTTCCC // |
Primer: | Sequence: RDB12323_A3Klb.seq |
>RDB12323_ADH_rev_E11_14.ab1 1 TGAGGAAACT CAAGGAATAA GAATTTTCGT TTTAAAACCT AAGAGTCACT TTAAAATTTG 61 TATACACTTA TTTTTTTTAT AACTTATTTA ATAATAAAAA TCATAAATCA TAAGAAATTC 121 GCTTATTTAG AAGTGGCGCG CCGGATCCTC TAGAGTCGAG ATCTCATGAT ATCACCACTT 181 TGTACAAGAA AGCTGAACGA GAAACGTAAA ATGATATAAA TATCAATATA TTAAATTAGA 241 TTTTGCATAA AAAACAGACT ACATAATACT GTAAAACACA ACATATCCAG TCACTATGGT 301 CGACCTGCAG ACTGGCTGTG TATAAGGGAG CCTGACATTT ATATTCCCCA GAACATCAGG 361 TTAATGGCGT TTTTGATGTC ATTTTCGCGG TGGCTGAGAT CAGCCACTTC TTCCCCGATA 421 ACGGAGACCG GCACACTGGC CATATCGGTG GTCATCATGC GCCAGCTTTC ATCCCCGATA 481 TGCACCACCG GGTAAAGTTC ACGGGAGACT TTATCTGACA GCAGACGTGC ACTGGCCAGG 541 GGGATCACCA TCCGTCGCCC GGGCGTGTCA ATAATATCAC TCTGTACATC CACAAACAGA 601 CGATAACGGC TCTCTCTTTT ATAGGTGTAA ACCTTAAACT GCATTTCACC AGCCCCTGTT 661 CTCGTCAGCA AAAGAGC // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
user report | Ito, D., Pericentrin-mediated SAS-6 recruitment promotes centriole assembly. Elife 8. pii: e41418 (2019). PMID 31182187. |
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2019.10.05
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