Resource data sheet
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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pARG1K-FFH1c (#RDB06437)

S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 promoter

Clone info. S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 promoter.
Comment Gateway compatible.
Vector backbone pUC119 (Plasmid)
Selectable markers E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Gene/insert name ccdB
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
 
Sequence RDB06437z.seq

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Nat. Biotechnol., 24, 841-847 (2006)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Nat. Biotechnol., 24, 841-847 (2006)) の引用と、寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
備考 このクローンの増殖にはSurvival2 (Invitrogen) あるいは同等の宿主を使用してください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06437 pARG1K-FFH1c DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pARG1K-FFH1c was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06437).

Reference section:

Matsuyama, A., Arai, R., Yashiroda, Y., Shirai, A., Kamata, A., Sekido, S., Kobayashi, Y., Hashimoto, A., Hamamoto, M., Hiraoka, Y., Horinouchi, S., Yoshida, M., ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe. Nat. Biotechnol., 24, 841-847 (2006). PMID 16823372. [link to RRC of NBRP]
Matsuyama, A., Arai, R., Yashiroda, Y., Shirai, A., Kamata, A., Sekido, S., Kobayashi, Y., Hashimoto, A., Hamamoto, M., Hiraoka, Y., Horinouchi, S., Yoshida, M., ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe. Nat. Biotechnol., 24, 841-847 (2006). PMID 16823372. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12268_A3J7.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer:
Sequence file: RDB12268_A3J7a.seq check
>06437_12268_A3J7_nmt1Fw_G07_19.ab1
    1 AGGAAATTTA TTAATCAGGA AACGTAACTC TCGGCTACTG GATGGTTCAG TCACCCAACG
   61 ATTACTGGGG AGAGAAAACA GGGCAAAAGC AAAGCTTAAA GGAATCCGAT TGTCATTCGG
  121 CAATGTGCAG CGAAACTAAA AACCGGATAA TGGACCTGTT AATCGAAACA TTGAAGATAT
  181 ATAAAGGAAG AGGAATCCTG GCATATCATC AATTGAATAA GTTGAATTAA TTATTTCAAT
  241 CTCATTCTCA CTTTCTGACT TATAGTCGCT TTGTTAAAGC TAGCGTCGAC AGGCCTGGAT
  301 CCGGATATCA ACAAGTTTGT ACAAAAAAGC TGAACGAGAA ACGTAAAATG ATATAAATAT
  361 CAATATATTA AATTAGATTT TGCATAAAAA ACAGACTACA TAATACTGTA AAACACAACA
  421 TATCCAGTCA CTATGGCGGC CGCATTAGGC ACCCCAGGCT TTACACTTTA TGCTTCCGGC
  481 TCGTATAATG TGTGGATTTT GAGTTAGGAT CCGTCGAGAT TTTCAGGAGC TAAGGAAGCT
  541 AAAATGGAGA AAAAAATCAC TGGATATACC ACCGTTGATA TATCCCAATG GCATCGTAAA
  601 GAACATTTTG AGGCATTTCA GTCAGTTGCT CAATGTACCT ATAACCAGAC CGTTCAGCTG
661 GATATTACGG CC
//
Primer:
Sequence file: RDB12268_A3J7b.seq check
>06437_12268_A3J7_ADHrev_H07_22.ab1
    1 GGGAGAGGTA ATCAAGGAAT AAGATTTTCG TTTTAAAACC TAAGAGTCAC TTTAAAATTT
   61 GTATACACTT ATTTTTTTTA TAACTTATTT AATAATAAAA ATCATAAATC ATAAGAAATT
  121 CGCTTATTTA GAAGTGGCGC GCCGGATCCT CTAGAGTCGA GATCTCAGTG GTGATGGTGG
  181 TGATGTTTGT CATCGTCGTC CTTGTAGTCC ATGGGTTTAT CGTCATCATC CTTATAATCC
  241 ATGATATCAA CCACTTTGTA CAAGAAAGCT GAACGAGAAA CGTAAAATGA TATAAATATC
  301 AATATATTAA ATTAGATTTT GCATAAAAAA CAGACTACAT AATACTGTAA AACACAACAT
  361 ATCCAGTCAC TATGGTCGAC CTGCAGACTG GCTGTGTATA AGGGAGCCTG ACATTTATAT
  421 TCCCCAGAAC ATCAGGTTAA TGGCGTTTTT GATGTCATTT TCGCGGTGGC TGAGATCAGC
  481 CACTTCTTCC CCGATAACGG AGACCGGCAC ACTGGCCATA TCGGTGGTCA TCATGCGCCA
  541 GCTTTCATCC CCGATATGCA CCACCGGGTA AAGTTCACGG GAGACTTTAT CTGACAGCAG
  601 ACGTGCACTG GCCAGGGGGA TCACCATCCG TCGCCCGGGC GTGTCAATAA TATCACTCTG
661 TACATCCACA AACAGA
//
Primer:
Sequence file: RDB12268_A3J7c.seq check
>06437_12268_A3J7_nmt1Fw2_A08_02.ab1
    1 GCCTTGGAGA CGATGACAAC ATCACCACCA TCACCACTGA GATCTCGACT CTAGAGGATC
   61 CGGCGCGCCA CTTCTAAATA AGCGAATTTC TTATGATTTA TGATTTTTAT TATTAAATAA
  121 GTTATAAAAA AAATAAGTGT ATACAAATTT TAAAGTGACT CTTAGGTTTT AAAACGAAAA
  181 TTCTTATTCT TGAGTAACTC TTTCCTGTAG GTCAGGTTGC TTTCTCAGGT ATAGTATGAG
  241 GTCGCTCTTA TTGACCACAC CTCTACCGGC AGATCCGCTA GGGATAACAG GGTAATATAC
  301 TAGTCGTAGC TGCTAAAGGT GAGGGCTCCT ATTTGTTTGA CAAGGAAGGT CGTAAATACA
  361 TTGACTTCAC CTCTGGCGTA GCTGTTACAT CTTTGGGTCA TGCTCATCCT GAAGTTGCTA
  421 GACTTGCCGC TGATCAATGC TCCAAATTAG TCCATAGTAG CAACCTTTTC TACAACGAGC
  481 CTGCCATTGA GCTATCTAAT GTCATTAATA ATTCTTTGGC AAAAAATAGC GGTATTGCTG
  541 GCCCCACAAA AATTTTCTTT GCCAATTGTG GTACTGAGGC CAATGAGACA GCCTTGAAGT
  601 TCGCTCGTAA GGCGGCCGCG GGCCCGCGGC CGCGTTTGAG AAGTATGGAG AAGGGAAGAG
661 TCAAATTGTT TACTT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Matsuyama, A., ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe. Nat. Biotechnol., 24, 841-847 (2006). PMID 16823372. [link to RRC of NBRP]