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pARG1K-FFH1c

S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 promoter

Catalog number RDB06437
Resource name pARG1K-FFH1c
Clone info. S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 promoter.
Gateway compatible.
Vector backbone pUC119 (Plasmid)
Selectable markers E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Gene/insert name ccdB
Depositor Yoshida, Minoru | Matsuyama, Akihisa |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Nat. Biotechnol., 24, 841-847 (2006)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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Material Transfer Agreement (MTA) [Word]
Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.
Distribution information (domestic users) [open/close]


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RDB06437 pARG1K-FFH1c DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB06437z.seq from Depositor

Original reference

original Matsuyama, A., ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe. Nat. Biotechnol., 24, 841-847 (2006). PMID 16823372.
original Matsuyama, A., ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe. Nat. Biotechnol., 24, 841-847 (2006). PMID 16823372.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12268_A3J7.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: | Sequence: RDB12268_A3J7a.seq
>06437_12268_A3J7_nmt1Fw_G07_19.ab1
    1 AGGAAATTTA TTAATCAGGA AACGTAACTC TCGGCTACTG GATGGTTCAG TCACCCAACG
   61 ATTACTGGGG AGAGAAAACA GGGCAAAAGC AAAGCTTAAA GGAATCCGAT TGTCATTCGG
  121 CAATGTGCAG CGAAACTAAA AACCGGATAA TGGACCTGTT AATCGAAACA TTGAAGATAT
  181 ATAAAGGAAG AGGAATCCTG GCATATCATC AATTGAATAA GTTGAATTAA TTATTTCAAT
  241 CTCATTCTCA CTTTCTGACT TATAGTCGCT TTGTTAAAGC TAGCGTCGAC AGGCCTGGAT
  301 CCGGATATCA ACAAGTTTGT ACAAAAAAGC TGAACGAGAA ACGTAAAATG ATATAAATAT
  361 CAATATATTA AATTAGATTT TGCATAAAAA ACAGACTACA TAATACTGTA AAACACAACA
  421 TATCCAGTCA CTATGGCGGC CGCATTAGGC ACCCCAGGCT TTACACTTTA TGCTTCCGGC
  481 TCGTATAATG TGTGGATTTT GAGTTAGGAT CCGTCGAGAT TTTCAGGAGC TAAGGAAGCT
  541 AAAATGGAGA AAAAAATCAC TGGATATACC ACCGTTGATA TATCCCAATG GCATCGTAAA
  601 GAACATTTTG AGGCATTTCA GTCAGTTGCT CAATGTACCT ATAACCAGAC CGTTCAGCTG
661 GATATTACGG CC
//
Primer: | Sequence: RDB12268_A3J7b.seq
>06437_12268_A3J7_ADHrev_H07_22.ab1
    1 GGGAGAGGTA ATCAAGGAAT AAGATTTTCG TTTTAAAACC TAAGAGTCAC TTTAAAATTT
   61 GTATACACTT ATTTTTTTTA TAACTTATTT AATAATAAAA ATCATAAATC ATAAGAAATT
  121 CGCTTATTTA GAAGTGGCGC GCCGGATCCT CTAGAGTCGA GATCTCAGTG GTGATGGTGG
  181 TGATGTTTGT CATCGTCGTC CTTGTAGTCC ATGGGTTTAT CGTCATCATC CTTATAATCC
  241 ATGATATCAA CCACTTTGTA CAAGAAAGCT GAACGAGAAA CGTAAAATGA TATAAATATC
  301 AATATATTAA ATTAGATTTT GCATAAAAAA CAGACTACAT AATACTGTAA AACACAACAT
  361 ATCCAGTCAC TATGGTCGAC CTGCAGACTG GCTGTGTATA AGGGAGCCTG ACATTTATAT
  421 TCCCCAGAAC ATCAGGTTAA TGGCGTTTTT GATGTCATTT TCGCGGTGGC TGAGATCAGC
  481 CACTTCTTCC CCGATAACGG AGACCGGCAC ACTGGCCATA TCGGTGGTCA TCATGCGCCA
  541 GCTTTCATCC CCGATATGCA CCACCGGGTA AAGTTCACGG GAGACTTTAT CTGACAGCAG
  601 ACGTGCACTG GCCAGGGGGA TCACCATCCG TCGCCCGGGC GTGTCAATAA TATCACTCTG
661 TACATCCACA AACAGA
//
Primer: | Sequence: RDB12268_A3J7c.seq
>06437_12268_A3J7_nmt1Fw2_A08_02.ab1
    1 GCCTTGGAGA CGATGACAAC ATCACCACCA TCACCACTGA GATCTCGACT CTAGAGGATC
   61 CGGCGCGCCA CTTCTAAATA AGCGAATTTC TTATGATTTA TGATTTTTAT TATTAAATAA
  121 GTTATAAAAA AAATAAGTGT ATACAAATTT TAAAGTGACT CTTAGGTTTT AAAACGAAAA
  181 TTCTTATTCT TGAGTAACTC TTTCCTGTAG GTCAGGTTGC TTTCTCAGGT ATAGTATGAG
  241 GTCGCTCTTA TTGACCACAC CTCTACCGGC AGATCCGCTA GGGATAACAG GGTAATATAC
  301 TAGTCGTAGC TGCTAAAGGT GAGGGCTCCT ATTTGTTTGA CAAGGAAGGT CGTAAATACA
  361 TTGACTTCAC CTCTGGCGTA GCTGTTACAT CTTTGGGTCA TGCTCATCCT GAAGTTGCTA
  421 GACTTGCCGC TGATCAATGC TCCAAATTAG TCCATAGTAG CAACCTTTTC TACAACGAGC
  481 CTGCCATTGA GCTATCTAAT GTCATTAATA ATTCTTTGGC AAAAAATAGC GGTATTGCTG
  541 GCCCCACAAA AATTTTCTTT GCCAATTGTG GTACTGAGGC CAATGAGACA GCCTTGAAGT
  601 TCGCTCGTAA GGCGGCCGCG GGCCCGCGGC CGCGTTTGAG AAGTATGGAG AAGGGAAGAG
661 TCAAATTGTT TACTT
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Matsuyama, A., ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe. Nat. Biotechnol., 24, 841-847 (2006). PMID 16823372.

2019.09.27

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