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pCMFlag_hsXBP1 (#RDB06272)

Expression vector of human XBP1.

Alternative name SET-0107_16
Clone info. Expression vector of human XBP1. Expression was confirmed by Western blotting with anti-FLAG antibody.
Comment Derived from Sugano BC000938. PCR cloning, forward primer: gtggtggtggcagccgcgccga; reverse primer: ttagttcattaatggcttccagcttg.
Vector backbone pCMV_S-FLAG (RDB05956) (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human XBP1 cDNA
Depositor|Developer DNA Bank, |
 
Remarks, protocol and/or map (pdf) RDB06272.pdf

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL and cite Gene 200, 149-156, 1997 in any publication.
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 発表に際し、理研BRCから入手したことを明記し、指定する文献(Gene, 200, 149-156 (1997))を引用すること。

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RDB06272 pCMFlag_hsXBP1 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCMFlag_hsXBP1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06272).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB06272_A3Ir.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer:
Sequence file: RDB06272_A3Ira.seq check
>06272_06272_A3Ir_CMV-Foward_C11_08.ab1
    1 TCTTCCCACT CCGCCCCATT GACGCAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
   61 TAAGCAGAGC TCTCTGGCTA ACTAGAGAAC CCACTGCTTA CTGGCTTATC GAAATTAATA
  121 CGACTCACTA TAGGGAGACC CAAGCTGAAT TCACCATGGA CTACAAGGAC GATGACGATA
  181 AGGCGGCCGT GGTGGTGGCA GCCGCGCCGA ACCCGGCCGA CGGGACCCCT AAAGTTCTGC
  241 TTCTGTCGGG GCAGCCCGCC TCCGCCGCCG GAGCCCCGGC CGGCCAGGCC CTGCCGCTCA
  301 TGGTGCCAGC CCAGAGAGGG GCCAGCCCGG AGGCAGCGAG CGGGGGGCTG CCCCAGGCGC
  361 GCAAGCGACA GCGCCTCACG CACCTGAGCC CCGAGGAGAA GGCGCTGAGG AGGAAACTGA
  421 AAAACAGAGT AGCAGCTCAG ACTGCCAGAG ATCGAAAGAA GGCTCGAATG AGTGAGCTGG
  481 AACAGCAAGT GGTAGATTTA GAAGAAGAGA ACCAAAAACT TTTGCTAGAA AATCAGCTTT
  541 TACGAGAGAA AACTCATGGC CTTGTAGTTG AGAACCAGGA GTTAAGACAG CGCTTGGGGA
  601 TGGATGCCCT GGTTGCTGAA GAGGAGGCGG AAGCCAAGGG GAATGAAGTG AGGCCAGTGG
661 CCGGGTCTGC
//
Primer:
Sequence file: RDB06272_A3Irb.seq check
>06272_06272_A3Ir_Sp6_D11_11.ab1
    1 CCATATAATC CATCCTCGAG CGGCTTAGTT CATTAATGGC TTCCAGCTTG GCTGATGACG
   61 TCCCCACTGA CAGAGAAAGG GAGGCTGGTA AGGAACTGGG TCCTTCTGGG TAGACCTCTG
  121 GGAGCTCCTC CAGGCTGGCA GGCTCTGGGG AAGGGCATTT GAAGAACATG ACTGGGTCCA
  181 AGTTGTCCAG AATGCCCAAC AGGATATCAG ACTCTGAATC TGAAGAGTCA ATACCGCCAG
  241 AATCCATGGG GAGATGTTCT GGAGGGGTGA CAACTGGGCC TGCACCTGCT GCAGAGGTGC
  301 ACGTAGTCTG AGTGCTGCGG ACTCAGCAGA CCCGGCCACT GGCCTCACTT CATTCCCCTT
  361 GGCTTCCGCC TCCTCTTCAG CAACCAGGGC ATCCATCCCC AAGCGCTGTC TTAACTCCTG
  421 GTTCTCAACT ACAAGGCCAT GAGTTTTCTC TCGTAAAAGC TGATTTTCTA GCAAAAGTTT
  481 TTGGTTCTCT TCTTCTAAAT CTACCACTTG CTGTTCCAGC TCACTCATTC GAGCCTTCTT
  541 TCGATCTCTG GCAGTCTGAG CTGCTACTCT GTTTTTCAGT TTCCTCCTCA GCGCCTTCTC
  601 CTCGGGGCTC AGGTGCGTGA GGCGCTGTCG CTTGCGCGCC TGGGGCAGCC CCCCGCTCGC
661 TGCCTCCGGG CTGGC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles