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pDUAL2-GFH41

S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with nmt1 (medium strength) promoter

Catalog number RDB06203
Resource name pDUAL2-GFH41
Clone info. S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with nmt1 (medium strength) promoter.
Multicopy marker, ura4+; Integration marker, ura4+, leu1-. Gateway incompatible.
Vector backbone pDUAL2 (Plasmid)
Selectable markers Amp^r
Reference of nucleotide sequence AB364069.1 (DDBJ accession number)
Depositor Yoshida, Minoru | Matsuyama, Akihisa |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Remarks ((Additional form)) GFP-A
Please use Schedule A (GFP-A) additionally.
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RDB06203 pDUAL2-GFH41 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB06203z.seq from Depositor

Featured content

Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

References

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14465_A6L7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw RDB14465_A6L7a.seq
>06203_14465_A6L7_nmt1Fw_F05_17_ABI24.ab1
    1 AGAAAATTTT ATTAATCAGG AAAACGTAAC TCTCGGCTAC TGGATGGTTC AGTCACCCAA
   61 CGATTACTGG GGAGAGAAAA CAGGGCAAAA GCAAAGCTTA AAGGAATCCG ATTGTCATTC
  121 GGCAATGTGC AGCGAAACTA AAAACCGGAT AATGGACCTG TTAATCGAAA CATTGAAGAT
  181 AAAGGAAGAG GAATCCTGGC ATATCATCAA TTGAATAAGT TGAATTAATT ATTTCAATCT
  241 CATTCTCACT TTCTGACTTA TAGTCGCTTT GTTAAAGCTA GCGTCGACAG GCCTGGATCC
  301 GGATATCATG AGTAAAGGAG AAGAACTTTT CACTGGAGTT GTCCCAATTC TTGTTGAATT
  361 AGATGGTGAT GTTAATGGGC ACAAATTTTC TGTCAGTGGA GAGGGTGAAG GTGATGCAAC
  421 ATACGGAAAA CTTACCCTTA AATTTATTTG CACTACTGGA AAACTACCTG TTCCGTGGCC
  481 AACACTTGTC ACTACTTTCT GTTATGGTGT TCAATGCTTT TCAAGATACC CAGATCACAT
  541 GAAACAGCAT GACTTTTTCA AGAGTGCCAT GCCCGAAGGC TATGTACAGG AAAGAACTAT
  601 ATTTTTCAAA GATGACGGGA ACTACAAGAC ACGTGCTGAA GTCAAGTTTG AAGGTGATAC
  661 CCTTGTTAAT AGAATCGAGT TAAAAGGTAT TGATTTTAAA GAAGATGGAA ACATTCTTGG
  721 ACACAAATTG GAATACAACT ATAACTCACA CAATGTATAC ATCATGGCAG ACAAACAAAA
  781 GAATGGAATC AAAGTTTAAC TTCAAAATTA GACACAACAT TGAAGATGGG AAGCGTTCAA
  841 CTAGCAGACC ATTATCAACA AATACTCCAA TTGGCGATGG GCCCTGTCCT TTACCAGACA
  901 CCATTACCTG TCCACACAAT CTGCCCTTTC GAAAGATCCA CGAAAAAGAG AAGACCACAT
  961 GGTTCCTTCT TGGAGTTTGT ACAGCTGGCT GGGGATACAC ACATGGCATG ATGACTATTA
 1021 CAAACTCAAT GGGAACTACA GGGACGACGA ATGGACCAAC CATCACCTAA CTATTACCAC
1081 TGGAATCTCG GACTCTTAGG AGAGATTCCG GGCGTCAAT
//
Primer: ADH_rev RDB14465_A6L7b.seq
>06203_14465_A6L7_ADH_rev_G05_20_ABI24.ab1
    1 GGGGGAGTCT CAAGATAAGA ATTTTCGTTT TAAACCTAAG AGTCACTTTA AAATTTGTAT
   61 ACACTTATTT TTTTTATAAC TTATTTAATA ATAAAAATCA TAAATCATAA GAAATTCGCT
  121 TATTTAGAAG TGGCGCGCCG GATCCTCTAG AGTCGAGATC TCAGTGGTGA TGGTGGTGAT
  181 GTTTGTCATC GTCGTCCTTG TAGTCCATGG GTTTGTATAG TTCATCCATG CCATGTGTAA
  241 TCCCAGCAGC TGTTACAAAC TCAAGAAGGA CCATGTGGTC TCTCTTTTCG TTGGGATCTT
  301 TCGAAAGGGC AGATTGTGTG GACAGGTAAT GGTTGTCTGG TAAAAGGACA GGGCCATCGC
  361 CAATTGGAGT ATTTTGTTGA TAATGGTCTG CTAGTTGAAC GCTTCCATCT TCAATGTTGT
  421 GTCTAATTTT GAAGTTAACT TTGATTCCAT TCTTTTGTTT GTCTGCCATG ATGTATACAT
  481 TGTGTGAGTT ATAGTTGTAT TCCAATTTGT GTCCAAGAAT GTTTCCATCT TCTTTAAAAT
  541 CAATACCTTT TAACTCGATT CTATTAACAA GGGTATCACC TTCAAACTTG ACTTCAGCAC
  601 GTGTCTTGTA GTTCCCGTCA TCTTTGAAAA ATATAGTTCT TTCCTGTACA TAGCCTTCGG
  661 GCATGGCACT CTTGAAAAAG TCATGCTGTT TCATGTGATC TGGGTATCTT GAAAAGCATT
  721 GAACACCATA ACAGAAAGTA GTGACAAGTG TTGGCCACGG AACAGGTAGT TTTCCAGTAG
  781 TGCAAATAAA TTTAAGGGTA AGTTTTCCGT ATGTTGCATC ACCTTCACCC TCTCCACTGA
  841 CAGAAAATTT GTGCCCATTA ACATCACCAT CTAATTCAAC AAGAATTGGG ACAACTCCCA
  901 GTGAAAAGTT CTTCTCCTTT ACTCATGATA TCCCGGGATC CAAGGGCCTG TTCGAACGCT
  961 AGCCTTTAAA CAAAGCGACT ATAAGTCAGA AAAGGTGAAG AATGGAGATT GAAATAAATT
 1021 TAATTCCAAC TTAATTCAAA TTGGATGATA TATGCCCAAG GGATCGTCTC CCTTTAATCT
1081 TCAAATGTTC CGAATTACAG GTTCAATTAT CCGGATTTTA AGATTTCCGC CGTGC
//
Primer: ADH-Forward RDB14465_A6L7c.seq
>06203_14465_A6L7_ADH-Forward_H05_23_ABI24.ab1
    1 TTTTCACTTA TTGACACACC TCTACCGGCA GATCCGCTAG GGATAACAGG GTAATATACT
   61 AGTGCCTCTA TTGATGCCTA TGGAACCCCT TTGACTGACG AGACTGTGAA GGCTTGTTTG
  121 GAAGCTGACG GTGTTCTTTT GGGTGCCGTT GGTGGTCCTG AATGGACCAA CCCCAATTGT
  181 CGTCCTGAGC AAGGTTTATT GAAGCTTCGT AAGAGTATGG GTGTTTGGGC CAACCTTCGA
  241 CCTTGCAACT TTGCCAGCAA GTCTTTAGTC AAGTACAGCC CTTTGAAGCC TGAAATCGTT
  301 GAAGGTGTCG ATTTTTGTGT TGTACGAGAA CTTACTGGAG GTTGTTACTT TGGTGAGCGC
  361 ACTGAGGACA ACGGATCGGG TTATGCTATG GACACTTGGC CTTACAGTTT GGAAGAAGTT
  421 TCTCGTATTG CTCGTTTGGC GGCCGCGGGC CCAACCTTCC AATTCATTAA ATCGAGTCCT
  481 CCATCAGATG ATTTACAAGG TTTTGCATAG AATCCCTAAG TAAGAGAAGT TGTTAGTGAT
  541 TTCTTCAAGT TTACTAAAAT TTACATACAA ATGGATGATC TCTTCTCCAT TGTTTCCTTT
  601 CTTCTTGCAA ACGCGTTTTA CAAAGAGATG ACATGCTATT TTCTGATTAT TTTTTTCTAT
  661 AGTTTTCTAT TTTGTACGTC CTTGGGAGTC CGAAATGTAA AATCGGGGTA TGGTTAGTAG
  721 CGGCAGCGTT CATTATGGTA TATAAAAGAT AAAAAGTAGG GGCAAGCGAG ACAGAATGGG
  781 ATACAAAGGG CATCGTCTAT AATTATAGCT AAAAATTGTA TTTTAATTTG TATTTTTTGT
  841 AATTTTATTT CTCATTGTTT TACTTAAAAT GAATGCGAAT TAGAGAAAAC TTCAACGAAT
  901 GTCAAAAATA AGCTCAGCAA AATATACAAT TTTAGGGAAG CGATCAGCAC TTATCTCGGT
  961 GTAGATTCCT TTTTTCTTCA TAAATCACCA TGTACCTGCG ATGGATTTTC TCTAGATGCC
1021 TAATAATTGA ACTATGGAGT ATATTACCAG TTGATAGTGA ACCTTAACTC GAAACGGA
//
Primer: f1ori_R3 RDB14465_A6L7d.seq
>06203_14465_A6L7_f1ori_R3_A06_03_ABI24.ab1
    1 GGATGAGCTT GAGTATGCGG TGTGAATACC GCACAGATGC GTAAGGAGAA AATACCGCAT
   61 CAGGCGCCAT TCGCCATTCA GGCTGCGCAA CTGTTGGGAA GGGCGATCGG TGCGGGCCTC
  121 TTCGCTATTA CGCCAGCTGG CGAAAGGGGG ATGTGCTGCA AGGCGATTAA GTTGGGTAAC
  181 GCCAGGGTTT TCCCAGTCAC GACGTTGTAA AACGACGGCC AGTGAATTCG AGCTCGGGAG
  241 CGCTACCGTG AATGGGCTCA ACCAAACAAT GAACCTTTTC TTCTGATTTT CCTACCACAC
  301 CGGAAAGGGA GGCAGAAGGC AAAAGGCCCA AGCTACCAGG AATGACAGAA GCCTCATCTG
  361 AAATAATGTC ACCAAACAAG TTGTCAGTCA AAACAACACC GTTAAGTGTA CGAGGGCTCT
  421 TGACCAAAAG CATGGCTGCG GAGTCAATGA GCTGGTTTTT TAAGGTAAGG TGAGGATATT
  481 CCTCCTTAAA AATCTTAGCT ACAGTCTTGC GCCAAAGACG AGAAGTTGCC AAAACATTAG
  541 CTTTGTCGAG TAATGTGACG GGAGCAGGAG GGTTGGAAGT TTCAGCTAAG CGGCCGCGGG
  601 CCCGAGTCTA ACTCCTTAAC CACTCGGACA TAGTGACTTA TCTGACACTC ATTGTAAATT
  661 AATATATATA TAGGCATTTT GTTTAGTTAA AGGTACTTAA GTAATTAGTA TAAACGAACC
  721 AATTTTATAA TCAGGAAGTT AAGTGAATGG TAGCACATGT CGTAAAAATT GTGAATTTTT
  781 ATTGAATAAT ATTTTAAATA CAAGCCTTTC TAGACTAGGT ATACTCATAA ACATATGAGC
  841 AAAAGGATAG AGGAGATTAC ATTGCATCTT CTACAAATTT ATTTATTGCC CTTTACTGAA
  901 AAATTAAATA ATGAGTACTA AATGATAAAA AGCGCTCAGT ACAAGAAAAG ATTGCAAAAA
  961 TATTGCATTC CTTCATGAAT TTAAAGTTGC ATATAACGCA TATTGAAGTA TAGTACCTAA
 1021 AACCAGGCAG TAGCGAAAAT TAATAGATAT ATTCGCAAGA ACAACTGGAC TGATTCAGAC
1081 ATGTAGATTT TTACCTATCC CTTC
//
Primer: Reverse2 RDB14465_A6L7e.seq
>06203_14465_A6L7_Reverse2_B06_06_ABI24.ab1
    1 CTTGAATGAT TACGCCAGCT TGTGATATTG ACGAAACTTT TTGACATCTA ATTTATTCTG
   61 TTCCAACACC AATGTTTATA ACCAAGTTTT ATCTTGTTTG TCTACATGGT ATTTTACATT
  121 CATCTACATA CATCTTTCAT TGGCTTTGTA CATAGTTATC ATTACAAGTC TAAAAAAATT
  181 CACTCTTTTC TTATTCAATG TCAATCCAAG AGAAAAGATT GTGGTAATGT TGTAGGAGCA
  241 TGTTTAATAA ATTACTATAG CAAATTACTT TTTATTCCCA AGGTGTTTAT CTATAATAGT
  301 TAATATTTTA GTCGCTACAT AAAATTTTAC CAAAGAGTAC TTGTATACTA ATTCTAAATG
  361 CCTTCTGACA TAAAACGCCT AGGAAAACAA ACGCAAACAA GGCATCGACT TTTTCAATAA
  421 CCAACCAAAA AAATTTTACA TTAGTCTTTT TTTAATGCTG AGAAAGTCTT TGCTGATATG
  481 CCTTCCAACC AGCTTCTCTA TATCTCTTGG CTTCGACAAC AGGATTACGA CCAGCTCCAT
  541 AGACTCCACG ACCAACAATG ATGATGTCGC TACCGCAGTT TACAATCACT TCTTCAGGAG
  601 TACGATATTG CTGTCCCAGC CCGTCTCCTT TAACATCCAA GCCGATACCA GGGGACATAG
  661 TTATGTAGTC GCTTTGAAGG TTAGGAAATC GACGACCAGC TATAAAGCCA AAGCAAAAAT
  721 CGGTATGCTT CTCAAACCAT TCTAAAGTTT TCTCTGTGTA GGAACCAGTA GCCAAAGAGC
  781 CTTTGGGAAG ACATTTCAGC CCAAAAGCAA GAGACCACGT CCCAAAAGTA AACCAACTTC
  841 TTTGAGCCTT GTATAATACC CTCGCCTGCA CTGTATGGCA ATTTGTGATA TGAGCCAGAG
  901 CATTTTTGTA CACACCAGAT GCATATGTAG CTGACGTATT TCATGTCTGC GATTTGCCGA
  961 TTCTTCAAAG AATAAGAAAC GATGCCATAA CTTAGCACAG TTCTCTACAT TCCTGTCGAA
1021 ATCTGACACG TCAATTTGCT CTGTACCGAC ATGTCCATTA CCTAGCGAT
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

2018.12.10

GNP_filter3_RDBDEP_html_181012.pl