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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pDUAL2-GFH41

S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with nmt1 (medium strength) promoter

Catalog number RDB06203
Resource name pDUAL2-GFH41
Clone info. S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with nmt1 (medium strength) promoter.
Comment Multicopy marker, ura4+; Integration marker, ura4+, leu1-. Gateway incompatible.
Vector backbone pDUAL2 (Plasmid)
Selectable markers Amp^r
Reference of insert sequence AB364069.1 (DDBJ accession number)
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、 寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB06203 pDUAL2-GFH41 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB06203z.seq

            Original reference

            original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB14465_A6L7p1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: nmt1Fw
            Sequence file: RDB14465_A6L7a.seq check
            >06203_14465_A6L7_nmt1Fw_F05_17_ABI24.ab1
                1 AGAAAATTTT ATTAATCAGG AAAACGTAAC TCTCGGCTAC TGGATGGTTC AGTCACCCAA
               61 CGATTACTGG GGAGAGAAAA CAGGGCAAAA GCAAAGCTTA AAGGAATCCG ATTGTCATTC
              121 GGCAATGTGC AGCGAAACTA AAAACCGGAT AATGGACCTG TTAATCGAAA CATTGAAGAT
              181 AAAGGAAGAG GAATCCTGGC ATATCATCAA TTGAATAAGT TGAATTAATT ATTTCAATCT
              241 CATTCTCACT TTCTGACTTA TAGTCGCTTT GTTAAAGCTA GCGTCGACAG GCCTGGATCC
              301 GGATATCATG AGTAAAGGAG AAGAACTTTT CACTGGAGTT GTCCCAATTC TTGTTGAATT
              361 AGATGGTGAT GTTAATGGGC ACAAATTTTC TGTCAGTGGA GAGGGTGAAG GTGATGCAAC
              421 ATACGGAAAA CTTACCCTTA AATTTATTTG CACTACTGGA AAACTACCTG TTCCGTGGCC
              481 AACACTTGTC ACTACTTTCT GTTATGGTGT TCAATGCTTT TCAAGATACC CAGATCACAT
              541 GAAACAGCAT GACTTTTTCA AGAGTGCCAT GCCCGAAGGC TATGTACAGG AAAGAACTAT
              601 ATTTTTCAAA GATGACGGGA ACTACAAGAC ACGTGCTGAA GTCAAGTTTG AAGGTGATAC
              661 CCTTGTTAAT AGAATCGAGT TAAAAGGTAT TGATTTTAAA GAAGATGGAA ACATTCTTGG
              721 ACACAAATTG GAATACAACT ATAACTCACA CAATGTATAC ATCATGGCAG ACAAACAAAA
              781 GAATGGAATC AAAGTTTAAC TTCAAAATTA GACACAACAT TGAAGATGGG AAGCGTTCAA
              841 CTAGCAGACC ATTATCAACA AATACTCCAA TTGGCGATGG GCCCTGTCCT TTACCAGACA
              901 CCATTACCTG TCCACACAAT CTGCCCTTTC GAAAGATCCA CGAAAAAGAG AAGACCACAT
              961 GGTTCCTTCT TGGAGTTTGT ACAGCTGGCT GGGGATACAC ACATGGCATG ATGACTATTA
             1021 CAAACTCAAT GGGAACTACA GGGACGACGA ATGGACCAAC CATCACCTAA CTATTACCAC
            1081 TGGAATCTCG GACTCTTAGG AGAGATTCCG GGCGTCAAT
            //
            Primer: ADH_rev
            Sequence file: RDB14465_A6L7b.seq check
            >06203_14465_A6L7_ADH_rev_G05_20_ABI24.ab1
                1 GGGGGAGTCT CAAGATAAGA ATTTTCGTTT TAAACCTAAG AGTCACTTTA AAATTTGTAT
               61 ACACTTATTT TTTTTATAAC TTATTTAATA ATAAAAATCA TAAATCATAA GAAATTCGCT
              121 TATTTAGAAG TGGCGCGCCG GATCCTCTAG AGTCGAGATC TCAGTGGTGA TGGTGGTGAT
              181 GTTTGTCATC GTCGTCCTTG TAGTCCATGG GTTTGTATAG TTCATCCATG CCATGTGTAA
              241 TCCCAGCAGC TGTTACAAAC TCAAGAAGGA CCATGTGGTC TCTCTTTTCG TTGGGATCTT
              301 TCGAAAGGGC AGATTGTGTG GACAGGTAAT GGTTGTCTGG TAAAAGGACA GGGCCATCGC
              361 CAATTGGAGT ATTTTGTTGA TAATGGTCTG CTAGTTGAAC GCTTCCATCT TCAATGTTGT
              421 GTCTAATTTT GAAGTTAACT TTGATTCCAT TCTTTTGTTT GTCTGCCATG ATGTATACAT
              481 TGTGTGAGTT ATAGTTGTAT TCCAATTTGT GTCCAAGAAT GTTTCCATCT TCTTTAAAAT
              541 CAATACCTTT TAACTCGATT CTATTAACAA GGGTATCACC TTCAAACTTG ACTTCAGCAC
              601 GTGTCTTGTA GTTCCCGTCA TCTTTGAAAA ATATAGTTCT TTCCTGTACA TAGCCTTCGG
              661 GCATGGCACT CTTGAAAAAG TCATGCTGTT TCATGTGATC TGGGTATCTT GAAAAGCATT
              721 GAACACCATA ACAGAAAGTA GTGACAAGTG TTGGCCACGG AACAGGTAGT TTTCCAGTAG
              781 TGCAAATAAA TTTAAGGGTA AGTTTTCCGT ATGTTGCATC ACCTTCACCC TCTCCACTGA
              841 CAGAAAATTT GTGCCCATTA ACATCACCAT CTAATTCAAC AAGAATTGGG ACAACTCCCA
              901 GTGAAAAGTT CTTCTCCTTT ACTCATGATA TCCCGGGATC CAAGGGCCTG TTCGAACGCT
              961 AGCCTTTAAA CAAAGCGACT ATAAGTCAGA AAAGGTGAAG AATGGAGATT GAAATAAATT
             1021 TAATTCCAAC TTAATTCAAA TTGGATGATA TATGCCCAAG GGATCGTCTC CCTTTAATCT
            1081 TCAAATGTTC CGAATTACAG GTTCAATTAT CCGGATTTTA AGATTTCCGC CGTGC
            //
            Primer: ADH-Forward
            Sequence file: RDB14465_A6L7c.seq check
            >06203_14465_A6L7_ADH-Forward_H05_23_ABI24.ab1
                1 TTTTCACTTA TTGACACACC TCTACCGGCA GATCCGCTAG GGATAACAGG GTAATATACT
               61 AGTGCCTCTA TTGATGCCTA TGGAACCCCT TTGACTGACG AGACTGTGAA GGCTTGTTTG
              121 GAAGCTGACG GTGTTCTTTT GGGTGCCGTT GGTGGTCCTG AATGGACCAA CCCCAATTGT
              181 CGTCCTGAGC AAGGTTTATT GAAGCTTCGT AAGAGTATGG GTGTTTGGGC CAACCTTCGA
              241 CCTTGCAACT TTGCCAGCAA GTCTTTAGTC AAGTACAGCC CTTTGAAGCC TGAAATCGTT
              301 GAAGGTGTCG ATTTTTGTGT TGTACGAGAA CTTACTGGAG GTTGTTACTT TGGTGAGCGC
              361 ACTGAGGACA ACGGATCGGG TTATGCTATG GACACTTGGC CTTACAGTTT GGAAGAAGTT
              421 TCTCGTATTG CTCGTTTGGC GGCCGCGGGC CCAACCTTCC AATTCATTAA ATCGAGTCCT
              481 CCATCAGATG ATTTACAAGG TTTTGCATAG AATCCCTAAG TAAGAGAAGT TGTTAGTGAT
              541 TTCTTCAAGT TTACTAAAAT TTACATACAA ATGGATGATC TCTTCTCCAT TGTTTCCTTT
              601 CTTCTTGCAA ACGCGTTTTA CAAAGAGATG ACATGCTATT TTCTGATTAT TTTTTTCTAT
              661 AGTTTTCTAT TTTGTACGTC CTTGGGAGTC CGAAATGTAA AATCGGGGTA TGGTTAGTAG
              721 CGGCAGCGTT CATTATGGTA TATAAAAGAT AAAAAGTAGG GGCAAGCGAG ACAGAATGGG
              781 ATACAAAGGG CATCGTCTAT AATTATAGCT AAAAATTGTA TTTTAATTTG TATTTTTTGT
              841 AATTTTATTT CTCATTGTTT TACTTAAAAT GAATGCGAAT TAGAGAAAAC TTCAACGAAT
              901 GTCAAAAATA AGCTCAGCAA AATATACAAT TTTAGGGAAG CGATCAGCAC TTATCTCGGT
              961 GTAGATTCCT TTTTTCTTCA TAAATCACCA TGTACCTGCG ATGGATTTTC TCTAGATGCC
            1021 TAATAATTGA ACTATGGAGT ATATTACCAG TTGATAGTGA ACCTTAACTC GAAACGGA
            //
            Primer: f1ori_R3
            Sequence file: RDB14465_A6L7d.seq check
            >06203_14465_A6L7_f1ori_R3_A06_03_ABI24.ab1
                1 GGATGAGCTT GAGTATGCGG TGTGAATACC GCACAGATGC GTAAGGAGAA AATACCGCAT
               61 CAGGCGCCAT TCGCCATTCA GGCTGCGCAA CTGTTGGGAA GGGCGATCGG TGCGGGCCTC
              121 TTCGCTATTA CGCCAGCTGG CGAAAGGGGG ATGTGCTGCA AGGCGATTAA GTTGGGTAAC
              181 GCCAGGGTTT TCCCAGTCAC GACGTTGTAA AACGACGGCC AGTGAATTCG AGCTCGGGAG
              241 CGCTACCGTG AATGGGCTCA ACCAAACAAT GAACCTTTTC TTCTGATTTT CCTACCACAC
              301 CGGAAAGGGA GGCAGAAGGC AAAAGGCCCA AGCTACCAGG AATGACAGAA GCCTCATCTG
              361 AAATAATGTC ACCAAACAAG TTGTCAGTCA AAACAACACC GTTAAGTGTA CGAGGGCTCT
              421 TGACCAAAAG CATGGCTGCG GAGTCAATGA GCTGGTTTTT TAAGGTAAGG TGAGGATATT
              481 CCTCCTTAAA AATCTTAGCT ACAGTCTTGC GCCAAAGACG AGAAGTTGCC AAAACATTAG
              541 CTTTGTCGAG TAATGTGACG GGAGCAGGAG GGTTGGAAGT TTCAGCTAAG CGGCCGCGGG
              601 CCCGAGTCTA ACTCCTTAAC CACTCGGACA TAGTGACTTA TCTGACACTC ATTGTAAATT
              661 AATATATATA TAGGCATTTT GTTTAGTTAA AGGTACTTAA GTAATTAGTA TAAACGAACC
              721 AATTTTATAA TCAGGAAGTT AAGTGAATGG TAGCACATGT CGTAAAAATT GTGAATTTTT
              781 ATTGAATAAT ATTTTAAATA CAAGCCTTTC TAGACTAGGT ATACTCATAA ACATATGAGC
              841 AAAAGGATAG AGGAGATTAC ATTGCATCTT CTACAAATTT ATTTATTGCC CTTTACTGAA
              901 AAATTAAATA ATGAGTACTA AATGATAAAA AGCGCTCAGT ACAAGAAAAG ATTGCAAAAA
              961 TATTGCATTC CTTCATGAAT TTAAAGTTGC ATATAACGCA TATTGAAGTA TAGTACCTAA
             1021 AACCAGGCAG TAGCGAAAAT TAATAGATAT ATTCGCAAGA ACAACTGGAC TGATTCAGAC
            1081 ATGTAGATTT TTACCTATCC CTTC
            //
            Primer: Reverse2
            Sequence file: RDB14465_A6L7e.seq check
            >06203_14465_A6L7_Reverse2_B06_06_ABI24.ab1
                1 CTTGAATGAT TACGCCAGCT TGTGATATTG ACGAAACTTT TTGACATCTA ATTTATTCTG
               61 TTCCAACACC AATGTTTATA ACCAAGTTTT ATCTTGTTTG TCTACATGGT ATTTTACATT
              121 CATCTACATA CATCTTTCAT TGGCTTTGTA CATAGTTATC ATTACAAGTC TAAAAAAATT
              181 CACTCTTTTC TTATTCAATG TCAATCCAAG AGAAAAGATT GTGGTAATGT TGTAGGAGCA
              241 TGTTTAATAA ATTACTATAG CAAATTACTT TTTATTCCCA AGGTGTTTAT CTATAATAGT
              301 TAATATTTTA GTCGCTACAT AAAATTTTAC CAAAGAGTAC TTGTATACTA ATTCTAAATG
              361 CCTTCTGACA TAAAACGCCT AGGAAAACAA ACGCAAACAA GGCATCGACT TTTTCAATAA
              421 CCAACCAAAA AAATTTTACA TTAGTCTTTT TTTAATGCTG AGAAAGTCTT TGCTGATATG
              481 CCTTCCAACC AGCTTCTCTA TATCTCTTGG CTTCGACAAC AGGATTACGA CCAGCTCCAT
              541 AGACTCCACG ACCAACAATG ATGATGTCGC TACCGCAGTT TACAATCACT TCTTCAGGAG
              601 TACGATATTG CTGTCCCAGC CCGTCTCCTT TAACATCCAA GCCGATACCA GGGGACATAG
              661 TTATGTAGTC GCTTTGAAGG TTAGGAAATC GACGACCAGC TATAAAGCCA AAGCAAAAAT
              721 CGGTATGCTT CTCAAACCAT TCTAAAGTTT TCTCTGTGTA GGAACCAGTA GCCAAAGAGC
              781 CTTTGGGAAG ACATTTCAGC CCAAAAGCAA GAGACCACGT CCCAAAAGTA AACCAACTTC
              841 TTTGAGCCTT GTATAATACC CTCGCCTGCA CTGTATGGCA ATTTGTGATA TGAGCCAGAG
              901 CATTTTTGTA CACACCAGAT GCATATGTAG CTGACGTATT TCATGTCTGC GATTTGCCGA
              961 TTCTTCAAAG AATAAGAAAC GATGCCATAA CTTAGCACAG TTCTCTACAT TCCTGTCGAA
            1021 ATCTGACACG TCAATTTGCT CTGTACCGAC ATGTCCATTA CCTAGCGAT
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

            2022.07.13

            GNP_filter3_RDBDEP_html_220713.pl