S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with nmt1 (medium strength) promoter
Clone info. | S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with nmt1 (medium strength) promoter. |
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Comment | Multicopy marker, ura4+; Integration marker, ura4+, leu1-. Gateway incompatible. |
Vector backbone | pDUAL2 (RDB06192) (Plasmid) |
Selectable markers | Amp^r |
Reference of insert sequence | AB364069.1 (DDBJ accession number) |
Depositor|Developer | Yoshida, Minoru | Matsuyama, Akihisa | |
Sequence | RDB06203z.seq |
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Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation. |
必要書類 | 提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、 寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB06203 | pDUAL2-GFH41 | DNA solution |
JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge |
Materials & Methods section:
The pDUAL2-GFH41 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06203). |
Reference section:
Matsuyama, A., Yoshida, M., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913. [link to RRC of NBRP] |
Matsuyama, A., Shirai, A., Yashiroda, Y., Kamata, A., Horinouchi, S., Yoshida, M., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB14465_A6L7p1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
Primer: nmt1Fw Sequence file: RDB14465_A6L7a.seq |
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>06203_14465_A6L7_nmt1Fw_F05_17_ABI24.ab1 1 AGAAAATTTT ATTAATCAGG AAAACGTAAC TCTCGGCTAC TGGATGGTTC AGTCACCCAA 61 CGATTACTGG GGAGAGAAAA CAGGGCAAAA GCAAAGCTTA AAGGAATCCG ATTGTCATTC 121 GGCAATGTGC AGCGAAACTA AAAACCGGAT AATGGACCTG TTAATCGAAA CATTGAAGAT 181 AAAGGAAGAG GAATCCTGGC ATATCATCAA TTGAATAAGT TGAATTAATT ATTTCAATCT 241 CATTCTCACT TTCTGACTTA TAGTCGCTTT GTTAAAGCTA GCGTCGACAG GCCTGGATCC 301 GGATATCATG AGTAAAGGAG AAGAACTTTT CACTGGAGTT GTCCCAATTC TTGTTGAATT 361 AGATGGTGAT GTTAATGGGC ACAAATTTTC TGTCAGTGGA GAGGGTGAAG GTGATGCAAC 421 ATACGGAAAA CTTACCCTTA AATTTATTTG CACTACTGGA AAACTACCTG TTCCGTGGCC 481 AACACTTGTC ACTACTTTCT GTTATGGTGT TCAATGCTTT TCAAGATACC CAGATCACAT 541 GAAACAGCAT GACTTTTTCA AGAGTGCCAT GCCCGAAGGC TATGTACAGG AAAGAACTAT 601 ATTTTTCAAA GATGACGGGA ACTACAAGAC ACGTGCTGAA GTCAAGTTTG AAGGTGATAC 661 CCTTGTTAAT AGAATCGAGT TAAAAGGTAT TGATTTTAAA GAAGATGGAA ACATTCTTGG 721 ACACAAATTG GAATACAACT ATAACTCACA CAATGTATAC ATCATGGCAG ACAAACAAAA 781 GAATGGAATC AAAGTTTAAC TTCAAAATTA GACACAACAT TGAAGATGGG AAGCGTTCAA 841 CTAGCAGACC ATTATCAACA AATACTCCAA TTGGCGATGG GCCCTGTCCT TTACCAGACA 901 CCATTACCTG TCCACACAAT CTGCCCTTTC GAAAGATCCA CGAAAAAGAG AAGACCACAT 961 GGTTCCTTCT TGGAGTTTGT ACAGCTGGCT GGGGATACAC ACATGGCATG ATGACTATTA 1021 CAAACTCAAT GGGAACTACA GGGACGACGA ATGGACCAAC CATCACCTAA CTATTACCAC 1081 TGGAATCTCG GACTCTTAGG AGAGATTCCG GGCGTCAAT // |
Primer: ADH_rev Sequence file: RDB14465_A6L7b.seq |
>06203_14465_A6L7_ADH_rev_G05_20_ABI24.ab1 1 GGGGGAGTCT CAAGATAAGA ATTTTCGTTT TAAACCTAAG AGTCACTTTA AAATTTGTAT 61 ACACTTATTT TTTTTATAAC TTATTTAATA ATAAAAATCA TAAATCATAA GAAATTCGCT 121 TATTTAGAAG TGGCGCGCCG GATCCTCTAG AGTCGAGATC TCAGTGGTGA TGGTGGTGAT 181 GTTTGTCATC GTCGTCCTTG TAGTCCATGG GTTTGTATAG TTCATCCATG CCATGTGTAA 241 TCCCAGCAGC TGTTACAAAC TCAAGAAGGA CCATGTGGTC TCTCTTTTCG TTGGGATCTT 301 TCGAAAGGGC AGATTGTGTG GACAGGTAAT GGTTGTCTGG TAAAAGGACA GGGCCATCGC 361 CAATTGGAGT ATTTTGTTGA TAATGGTCTG CTAGTTGAAC GCTTCCATCT TCAATGTTGT 421 GTCTAATTTT GAAGTTAACT TTGATTCCAT TCTTTTGTTT GTCTGCCATG ATGTATACAT 481 TGTGTGAGTT ATAGTTGTAT TCCAATTTGT GTCCAAGAAT GTTTCCATCT TCTTTAAAAT 541 CAATACCTTT TAACTCGATT CTATTAACAA GGGTATCACC TTCAAACTTG ACTTCAGCAC 601 GTGTCTTGTA GTTCCCGTCA TCTTTGAAAA ATATAGTTCT TTCCTGTACA TAGCCTTCGG 661 GCATGGCACT CTTGAAAAAG TCATGCTGTT TCATGTGATC TGGGTATCTT GAAAAGCATT 721 GAACACCATA ACAGAAAGTA GTGACAAGTG TTGGCCACGG AACAGGTAGT TTTCCAGTAG 781 TGCAAATAAA TTTAAGGGTA AGTTTTCCGT ATGTTGCATC ACCTTCACCC TCTCCACTGA 841 CAGAAAATTT GTGCCCATTA ACATCACCAT CTAATTCAAC AAGAATTGGG ACAACTCCCA 901 GTGAAAAGTT CTTCTCCTTT ACTCATGATA TCCCGGGATC CAAGGGCCTG TTCGAACGCT 961 AGCCTTTAAA CAAAGCGACT ATAAGTCAGA AAAGGTGAAG AATGGAGATT GAAATAAATT 1021 TAATTCCAAC TTAATTCAAA TTGGATGATA TATGCCCAAG GGATCGTCTC CCTTTAATCT 1081 TCAAATGTTC CGAATTACAG GTTCAATTAT CCGGATTTTA AGATTTCCGC CGTGC // |
Primer: ADH-Forward Sequence file: RDB14465_A6L7c.seq |
>06203_14465_A6L7_ADH-Forward_H05_23_ABI24.ab1 1 TTTTCACTTA TTGACACACC TCTACCGGCA GATCCGCTAG GGATAACAGG GTAATATACT 61 AGTGCCTCTA TTGATGCCTA TGGAACCCCT TTGACTGACG AGACTGTGAA GGCTTGTTTG 121 GAAGCTGACG GTGTTCTTTT GGGTGCCGTT GGTGGTCCTG AATGGACCAA CCCCAATTGT 181 CGTCCTGAGC AAGGTTTATT GAAGCTTCGT AAGAGTATGG GTGTTTGGGC CAACCTTCGA 241 CCTTGCAACT TTGCCAGCAA GTCTTTAGTC AAGTACAGCC CTTTGAAGCC TGAAATCGTT 301 GAAGGTGTCG ATTTTTGTGT TGTACGAGAA CTTACTGGAG GTTGTTACTT TGGTGAGCGC 361 ACTGAGGACA ACGGATCGGG TTATGCTATG GACACTTGGC CTTACAGTTT GGAAGAAGTT 421 TCTCGTATTG CTCGTTTGGC GGCCGCGGGC CCAACCTTCC AATTCATTAA ATCGAGTCCT 481 CCATCAGATG ATTTACAAGG TTTTGCATAG AATCCCTAAG TAAGAGAAGT TGTTAGTGAT 541 TTCTTCAAGT TTACTAAAAT TTACATACAA ATGGATGATC TCTTCTCCAT TGTTTCCTTT 601 CTTCTTGCAA ACGCGTTTTA CAAAGAGATG ACATGCTATT TTCTGATTAT TTTTTTCTAT 661 AGTTTTCTAT TTTGTACGTC CTTGGGAGTC CGAAATGTAA AATCGGGGTA TGGTTAGTAG 721 CGGCAGCGTT CATTATGGTA TATAAAAGAT AAAAAGTAGG GGCAAGCGAG ACAGAATGGG 781 ATACAAAGGG CATCGTCTAT AATTATAGCT AAAAATTGTA TTTTAATTTG TATTTTTTGT 841 AATTTTATTT CTCATTGTTT TACTTAAAAT GAATGCGAAT TAGAGAAAAC TTCAACGAAT 901 GTCAAAAATA AGCTCAGCAA AATATACAAT TTTAGGGAAG CGATCAGCAC TTATCTCGGT 961 GTAGATTCCT TTTTTCTTCA TAAATCACCA TGTACCTGCG ATGGATTTTC TCTAGATGCC 1021 TAATAATTGA ACTATGGAGT ATATTACCAG TTGATAGTGA ACCTTAACTC GAAACGGA // |
Primer: f1ori_R3 Sequence file: RDB14465_A6L7d.seq |
>06203_14465_A6L7_f1ori_R3_A06_03_ABI24.ab1 1 GGATGAGCTT GAGTATGCGG TGTGAATACC GCACAGATGC GTAAGGAGAA AATACCGCAT 61 CAGGCGCCAT TCGCCATTCA GGCTGCGCAA CTGTTGGGAA GGGCGATCGG TGCGGGCCTC 121 TTCGCTATTA CGCCAGCTGG CGAAAGGGGG ATGTGCTGCA AGGCGATTAA GTTGGGTAAC 181 GCCAGGGTTT TCCCAGTCAC GACGTTGTAA AACGACGGCC AGTGAATTCG AGCTCGGGAG 241 CGCTACCGTG AATGGGCTCA ACCAAACAAT GAACCTTTTC TTCTGATTTT CCTACCACAC 301 CGGAAAGGGA GGCAGAAGGC AAAAGGCCCA AGCTACCAGG AATGACAGAA GCCTCATCTG 361 AAATAATGTC ACCAAACAAG TTGTCAGTCA AAACAACACC GTTAAGTGTA CGAGGGCTCT 421 TGACCAAAAG CATGGCTGCG GAGTCAATGA GCTGGTTTTT TAAGGTAAGG TGAGGATATT 481 CCTCCTTAAA AATCTTAGCT ACAGTCTTGC GCCAAAGACG AGAAGTTGCC AAAACATTAG 541 CTTTGTCGAG TAATGTGACG GGAGCAGGAG GGTTGGAAGT TTCAGCTAAG CGGCCGCGGG 601 CCCGAGTCTA ACTCCTTAAC CACTCGGACA TAGTGACTTA TCTGACACTC ATTGTAAATT 661 AATATATATA TAGGCATTTT GTTTAGTTAA AGGTACTTAA GTAATTAGTA TAAACGAACC 721 AATTTTATAA TCAGGAAGTT AAGTGAATGG TAGCACATGT CGTAAAAATT GTGAATTTTT 781 ATTGAATAAT ATTTTAAATA CAAGCCTTTC TAGACTAGGT ATACTCATAA ACATATGAGC 841 AAAAGGATAG AGGAGATTAC ATTGCATCTT CTACAAATTT ATTTATTGCC CTTTACTGAA 901 AAATTAAATA ATGAGTACTA AATGATAAAA AGCGCTCAGT ACAAGAAAAG ATTGCAAAAA 961 TATTGCATTC CTTCATGAAT TTAAAGTTGC ATATAACGCA TATTGAAGTA TAGTACCTAA 1021 AACCAGGCAG TAGCGAAAAT TAATAGATAT ATTCGCAAGA ACAACTGGAC TGATTCAGAC 1081 ATGTAGATTT TTACCTATCC CTTC // |
Primer: Reverse2 Sequence file: RDB14465_A6L7e.seq |
>06203_14465_A6L7_Reverse2_B06_06_ABI24.ab1 1 CTTGAATGAT TACGCCAGCT TGTGATATTG ACGAAACTTT TTGACATCTA ATTTATTCTG 61 TTCCAACACC AATGTTTATA ACCAAGTTTT ATCTTGTTTG TCTACATGGT ATTTTACATT 121 CATCTACATA CATCTTTCAT TGGCTTTGTA CATAGTTATC ATTACAAGTC TAAAAAAATT 181 CACTCTTTTC TTATTCAATG TCAATCCAAG AGAAAAGATT GTGGTAATGT TGTAGGAGCA 241 TGTTTAATAA ATTACTATAG CAAATTACTT TTTATTCCCA AGGTGTTTAT CTATAATAGT 301 TAATATTTTA GTCGCTACAT AAAATTTTAC CAAAGAGTAC TTGTATACTA ATTCTAAATG 361 CCTTCTGACA TAAAACGCCT AGGAAAACAA ACGCAAACAA GGCATCGACT TTTTCAATAA 421 CCAACCAAAA AAATTTTACA TTAGTCTTTT TTTAATGCTG AGAAAGTCTT TGCTGATATG 481 CCTTCCAACC AGCTTCTCTA TATCTCTTGG CTTCGACAAC AGGATTACGA CCAGCTCCAT 541 AGACTCCACG ACCAACAATG ATGATGTCGC TACCGCAGTT TACAATCACT TCTTCAGGAG 601 TACGATATTG CTGTCCCAGC CCGTCTCCTT TAACATCCAA GCCGATACCA GGGGACATAG 661 TTATGTAGTC GCTTTGAAGG TTAGGAAATC GACGACCAGC TATAAAGCCA AAGCAAAAAT 721 CGGTATGCTT CTCAAACCAT TCTAAAGTTT TCTCTGTGTA GGAACCAGTA GCCAAAGAGC 781 CTTTGGGAAG ACATTTCAGC CCAAAAGCAA GAGACCACGT CCCAAAAGTA AACCAACTTC 841 TTTGAGCCTT GTATAATACC CTCGCCTGCA CTGTATGGCA ATTTGTGATA TGAGCCAGAG 901 CATTTTTGTA CACACCAGAT GCATATGTAG CTGACGTATT TCATGTCTGC GATTTGCCGA 961 TTCTTCAAAG AATAAGAAAC GATGCCATAA CTTAGCACAG TTCTCTACAT TCCTGTCGAA 1021 ATCTGACACG TCAATTTGCT CTGTACCGAC ATGTCCATTA CCTAGCGAT // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913. [link to RRC of NBRP] |
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original | Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. [link to RRC of NBRP] |
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