RDB06199 - pDUAL2-FFH1

Resource data sheet
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pDUAL2-FFH1

S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 promoter

Catalog number	RDB06199
Resource name	pDUAL2-FFH1
Clone info.	S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 promoter. Multicopy marker, ura4+; Integration marker, ura4+, leu1-. Gateway incompatible.
Vector backbone	pDUAL2 (Plasmid)
Selectable markers	Amp^r
Reference of nucleotide sequence	AB364065.1 (DDBJ accession number)
Depositor	Yoshida, Minoru \| Matsuyama, Akihisa \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.

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Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
Ordering	Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、　寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
提供依頼	手続きの詳細は、「提供申込みについて」をご覧ください。提供依頼書 [Word] 提供同意書 [Word]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB06199	pDUAL2-FFH1	DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file	Sequence RDB06199z.seq from Depositor

Featured content	Expression Vector Backbone of Schizosaccharomyces pombe (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet	RDB14529_A6L7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw	RDB14529_A6L7a.seq
>06199_14529_3_A6L7_nmt1Fw_G04_07_ABI08.ab1 1 TGAAATTAAT AAATCAGGAA ACGTAACTCT CGGCTACTGG ATGGTTCAGT CACCCAACGA 61 TTACTGGGGA GAGAAAACAG GGCAAAAGCA AAGCTTAAAG GAATCCGATT GTCATTCGGC 121 AATGTGCAGC GAAACTAAAA ACCGGATAAT GGACCTGTTA ATCGAAACAT TGAAGATATA 181 TAAAGGAAGA GGAATCCTGG CATATCATCA ATTGAATAAG TTGAATTAAT TATTTCAATC 241 TCATTCTCAC TTTCTGACTT ATAGTCGCTT TGTTAAAGCT AGCGTCGACA GGCCTGGATC 301 CGGATATCAT GGATTATAAG GATGATGACG ATAAACCCAT GGACTACAAG GACGACGATG 361 ACAAACATCA CCACCATCAC CACTGAGATC TCGACTCTAG AGGATCCGGC GCGCCACTTC 421 TAAATAAGCG AATTTCTTAT GATTTATGAT TTTTATTATT AAATAAGTTA TAAAAAAAAT 481 AAGTGTATAC AAATTTTAAA GTGACTCTTA GGTTTTAAAA CGAAAATTCT TATTCTTGAG 541 TAACTCTTTC CTGTAGGTCA GGTTGCTTTC TCAGGTATAG TATGAGGTCG CTCTTATTGA 601 CCACACCTCT ACCGGCAGAT CCGCTAGGGA TAACAGGGTA ATATACTAGT GCCTCTATTG 661 ATGCCTATGG AACCCCTTTG ACTGACGAGA CTGTGAAGGC TTGTTTGGAA GCTGACGGTG 721 TTCTTTTGGG TGCCGTTGGT GGTCCTGAAT GGACCAACCC CAATTGTCGT CCTGAGCAAG 781 GTTTATTGAA GCTTCGTAAG AGTATGGGTG TTTGGGCCAA CCTTCGACCT TGCAACTTTG 841 CCAGCAAGTC TTTAGTCAAG TACAGCCCTT TGAAGCCTGA AATCGTTGAA GGTGTCGATT 901 TTTTGTGTTG TACGAGAACT TTACTGGAGG TTGTTACTTT GGGTGAGCGC ACTGAGGACA 961 ACGGGATCGG GGTTATGCTA TGGACACTTT GGTCCTTTAC AGTTTGGCAA GGAAGTTTCT 1021 CGTATGCTCG TTTGCGCCGC GACCATCCTT CCAATTCATT AATCGAGTTC GTCATCAGAT 1081 GATTTTAGAC GGAT //
Primer: ADH-Forward	RDB14529_A6L7b.seq
>06199_14529_3_A6L7_ADH-Forward_H04_08_ABI08.ab1 1 ACCTCTCTTA TTGACACACC TCTACCGGCA GATCCGCTAG GGATAACAGG GTAATATACT 61 AGTGCCTCTA TTGATGCCTA TGGAACCCCT TTGACTGACG AGACTGTGAA GGCTTGTTTG 121 GAAGCTGACG GTGTTCTTTT GGGTGCCGTT GGTGGTCCTG AATGGACCAA CCCCAATTGT 181 CGTCCTGAGC AAGGTTTATT GAAGCTTCGT AAGAGTATGG GTGTTTGGGC CAACCTTCGA 241 CCTTGCAACT TTGCCAGCAA GTCTTTAGTC AAGTACAGCC CTTTGAAGCC TGAAATCGTT 301 GAAGGTGTCG ATTTTTGTGT TGTACGAGAA CTTACTGGAG GTTGTTACTT TGGTGAGCGC 361 ACTGAGGACA ACGGATCGGG TTATGCTATG GACACTTGGC CTTACAGTTT GGAAGAAGTT 421 TCTCGTATTG CTCGTTTGGC GGCCGCGGGC CCAACCTTCC AATTCATTAA ATCGAGTCCT 481 CCATCAGATG ATTTACAAGG TTTTGCATAG AATCCCTAAG TAAGAGAAGT TGTTAGTGAT 541 TTCTTCAAGT TTACTAAAAT TTACATACAA ATGGATGATC TCTTCTCCAT TGTTTCCTTT 601 CTTCTTGCAA ACGCGTTTTA CAAAGAGATG ACATGCTATT TTCTGATTAT TTTTTTCTAT 661 AGTTTTCTAT TTTGTACGTC CTTGGGAGTC CGAAATGTAA AATCGGGGTA TGGTTAGTAG 721 CGGCAGCGTT CATTATGGTA TATAAAAGAT AAAAAGTATG GGCAAGCGAG ACAGAATGGG 781 ATACAAAGGG CATCGTCTAT AATTATAGCT AAAAATTGTA TTTTAATTTG TATTTTTTGT 841 AATTTTAATT TCTCATTGTT TTACTTAAAA TGAATGCGAA TTAGAGAAAA ACTTTCACGA 901 ATGTCAAAAT AAGCTTCAGC AAAAATATAA CAATTTTAGG AAAGGCGATC AGCCACTTAT 961 TCTCGGTGGT AGATTCCTTT TCCTCCATAA TTCGAACAAT GTACTGGCCG ATGGATTTTC 1021 TCTTAGAATG CCTAATAATT GGTACTATGA TGATAGCCAG TTGATAG //
Primer: f1ori_R3	RDB14529_A6L7c.seq
>06199_14529_3_A6L7_f1ori_R3_A05_01_ABI08.ab1 1 GCCGATGGGT GTCCTTTGCG TATGCGGTGT GAATACCGCA CAGATGCGTA AGGAGAAAAT 61 ACCGCATCAG GCGCCATTCG CCATTCAGGC TGCGCAACTG TTGGGAAGGG CGATCGGTGC 121 GGGCCTCTTC GCTATTACGC CAGCTGGCGA AAGGGGGATG TGCTGCAAGG CGATTAAGTT 181 GGGTAACGCC AGGGTTTTCC CAGTCACGAC GTTGTAAAAC GACGGCCAGT GAATTCGAGC 241 TCGGGAGCGC TACCGTGAAT GGGCTCAACC AAACAATGAA CCTTTTCTTC TGATTTTCCT 301 ACCACACCGG AAAGGGAGGC AGAAGGCAAA AGGCCCAAGC TACCAGGAAT GACAGAAGCC 361 TCATCTGAAA TAATGTCACC AAACAAGTTG TCAGTCAAAA CAACACCGTT AAGTGTACGA 421 GGGCTCTTGA CCAAAAGCAT GGCTGCGGAG TCAATGAGCT GGTTTTTTAA GGTAAGGTGA 481 GGATATTCCT CCTTAAAAAT CTTAGCTACA GTCTTGCGCC AAAGACGAGA AGTTGCCAAA 541 ACATTAGCTT TGTCGAGTAA TGTGACGGGA GCAGGAGGGT TGGAAGTTTC AGCTAAGCGG 601 CCGCGGGCCC GAGTCTAACT CCTTAACCAC TCGGACATAG TGACTTATCT GACACTCATT 661 GTAAATTAAT ATATATATAG GCATTTTGTT TAGTTAAAGG TACTTAAGTA ATTAGTATAA 721 ACGAACCAAT TTTATAATCA GGAAGTTAAG TGAATGGTAG CACATGTCGT AAAAATTGTG 781 AATTTTTATT GAATAATATT TTAAATACAA GCCTTTCTAG ACTAGGTATA CTCATAAACA 841 TATGAGCAAA AGGATAGAGG AGATTACATT GCATCTTCTA CAAATTTATT TATTGCCCTT 901 TACTGAAAAA TTAAATAATG AGTACTAAAT GATAAAAAAG CGCTCAGTAC AAGAAAGATT 961 GCAAAAATAT TGCATTCTTT CATGAATTAA GTTGGCATAT AAGCATAATT GAAGGTTAAT 1021 AGTTACTAAA ACAGCAGTTA GCCGAAATAT AGGATTAATA TTCGCAGAAA TTGTGCACAT 1081 AAGTTGAAGG AAATGTGTAC GA //
Primer: Reverse2	RDB14529_A6L7d.seq
>06199_14529_3_A6L7_Reverse2_B05_02_ABI08.ab1 1 AGGGCCATGC ATTCGCCAAG CTTGTGATAT TGACGAAACT TTTTGACATC TAATTTATTC 61 TGTTCCAACA CCAATGTTTA TAACCAAGTT TTATCTTGTT TGTCTACATG GTATTTTACA 121 TTCATCTACA TACATCTTTC ATTGGCTTTG TACATAGTTA TCATTACAAG TCTAAAAAAA 181 TTCACTCTTT TCTTATTCAA TGTCAATCCA AGAGAAAAGA TTGTGGTAAT GTTGTAGGAG 241 CATGTTTAAT AAATTACTAT AGCAAATTAC TTTTTATTCC CAAGGTGTTT ATCTATAATA 301 GTTAATATTT TAGTCGCTAC ATAAAATTTT ACCAAAGAGT ACTTGTATAC TAATTCTAAA 361 TGCCTTCTGA CATAAAACGC CTAGGAAAAC AAACGCAAAC AAGGCATCGA CTTTTTCAAT 421 AACCAACCAA AAAAATTTTA CATTAGTCTT TTTTTAATGC TGAGAAAGTC TTTGCTGATA 481 TGCCTTCCAA CCAGCTTCTC TATATCTCTT GGCTTCGACA ACAGGATTAC GACCAGCTCC 541 ATAGACTCCA CGACCAACAA TGATGATGTC GCTACCGCAG TTTACAATCA CTTCTTCAGG 601 AGTACGATAT TGCTGTCCCA GCCCGTCTCC TTTAACATCC AAGCCGATAC CAGGGGACAT 661 AGTTATGTAG TCGCTTTGAA GGTTAGGAAA TCGACGACCA GCTATAAAGC CAAAGCAAAA 721 ATCGGTATGC TTCTCAAACC ATTCTAAGGT TTTCTCTGTG TAGGAACCAG TAGCCAAAGA 781 GCCTTTGGAA GACATTTCAG CCAAAAGCAA GAGACCACGT CCCAAAGGTA AACCAACTTC 841 TTTGAGGCCT TGTATAATAC CCTCGCCTGG CACTGTATGG CAATTTGTGA TATGAGCCCA 901 AGAAGCAATT TTGTACACAC CAGATGCATA TTGTAGCTTT GACGTATTTT CCCAATGTCT 961 GCGAATTTGG CGATCCTCAA GATAAGAAAC GATGCTTTTT TACCTAAGGC AACAAGTTTT 1021 TCTACCATAT TCCTTGTTCG GAATCCTCGA CCAACCGTCG ATATGGTCGT GATTAGCCAG 1081 GAGTATGGTT CCGT //

Please visit Sequencing and PCR primers for primer information.

References

original	Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
original	Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

2018.12.10

GNP_filter3_RDBDEP_html_181012.pl