Please review the QC test results indicated by icon below as well as clone information before placing your order.

pDUAL2-FFH1

S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 promoter

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.

Ordering

Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、　寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14529_A6L7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw Sequence file: RDB14529_A6L7a.seq
>06199_14529_3_A6L7_nmt1Fw_G04_07_ABI08.ab1     1 TGAAATTAAT AAATCAGGAA ACGTAACTCT CGGCTACTGG ATGGTTCAGT CACCCAACGA    61 TTACTGGGGA GAGAAAACAG GGCAAAAGCA AAGCTTAAAG GAATCCGATT GTCATTCGGC   121 AATGTGCAGC GAAACTAAAA ACCGGATAAT GGACCTGTTA ATCGAAACAT TGAAGATATA   181 TAAAGGAAGA GGAATCCTGG CATATCATCA ATTGAATAAG TTGAATTAAT TATTTCAATC   241 TCATTCTCAC TTTCTGACTT ATAGTCGCTT TGTTAAAGCT AGCGTCGACA GGCCTGGATC   301 CGGATATCAT GGATTATAAG GATGATGACG ATAAACCCAT GGACTACAAG GACGACGATG   361 ACAAACATCA CCACCATCAC CACTGAGATC TCGACTCTAG AGGATCCGGC GCGCCACTTC   421 TAAATAAGCG AATTTCTTAT GATTTATGAT TTTTATTATT AAATAAGTTA TAAAAAAAAT   481 AAGTGTATAC AAATTTTAAA GTGACTCTTA GGTTTTAAAA CGAAAATTCT TATTCTTGAG   541 TAACTCTTTC CTGTAGGTCA GGTTGCTTTC TCAGGTATAG TATGAGGTCG CTCTTATTGA   601 CCACACCTCT ACCGGCAGAT CCGCTAGGGA TAACAGGGTA ATATACTAGT GCCTCTATTG   661 ATGCCTATGG AACCCCTTTG ACTGACGAGA CTGTGAAGGC TTGTTTGGAA GCTGACGGTG   721 TTCTTTTGGG TGCCGTTGGT GGTCCTGAAT GGACCAACCC CAATTGTCGT CCTGAGCAAG   781 GTTTATTGAA GCTTCGTAAG AGTATGGGTG TTTGGGCCAA CCTTCGACCT TGCAACTTTG   841 CCAGCAAGTC TTTAGTCAAG TACAGCCCTT TGAAGCCTGA AATCGTTGAA GGTGTCGATT   901 TTTTGTGTTG TACGAGAACT TTACTGGAGG TTGTTACTTT GGGTGAGCGC ACTGAGGACA   961 ACGGGATCGG GGTTATGCTA TGGACACTTT GGTCCTTTAC AGTTTGGCAA GGAAGTTTCT  1021 CGTATGCTCG TTTGCGCCGC GACCATCCTT CCAATTCATT AATCGAGTTC GTCATCAGAT 1081 GATTTTAGAC GGAT //
Primer: ADH-Forward Sequence file: RDB14529_A6L7b.seq
>06199_14529_3_A6L7_ADH-Forward_H04_08_ABI08.ab1     1 ACCTCTCTTA TTGACACACC TCTACCGGCA GATCCGCTAG GGATAACAGG GTAATATACT    61 AGTGCCTCTA TTGATGCCTA TGGAACCCCT TTGACTGACG AGACTGTGAA GGCTTGTTTG   121 GAAGCTGACG GTGTTCTTTT GGGTGCCGTT GGTGGTCCTG AATGGACCAA CCCCAATTGT   181 CGTCCTGAGC AAGGTTTATT GAAGCTTCGT AAGAGTATGG GTGTTTGGGC CAACCTTCGA   241 CCTTGCAACT TTGCCAGCAA GTCTTTAGTC AAGTACAGCC CTTTGAAGCC TGAAATCGTT   301 GAAGGTGTCG ATTTTTGTGT TGTACGAGAA CTTACTGGAG GTTGTTACTT TGGTGAGCGC   361 ACTGAGGACA ACGGATCGGG TTATGCTATG GACACTTGGC CTTACAGTTT GGAAGAAGTT   421 TCTCGTATTG CTCGTTTGGC GGCCGCGGGC CCAACCTTCC AATTCATTAA ATCGAGTCCT   481 CCATCAGATG ATTTACAAGG TTTTGCATAG AATCCCTAAG TAAGAGAAGT TGTTAGTGAT   541 TTCTTCAAGT TTACTAAAAT TTACATACAA ATGGATGATC TCTTCTCCAT TGTTTCCTTT   601 CTTCTTGCAA ACGCGTTTTA CAAAGAGATG ACATGCTATT TTCTGATTAT TTTTTTCTAT   661 AGTTTTCTAT TTTGTACGTC CTTGGGAGTC CGAAATGTAA AATCGGGGTA TGGTTAGTAG   721 CGGCAGCGTT CATTATGGTA TATAAAAGAT AAAAAGTATG GGCAAGCGAG ACAGAATGGG   781 ATACAAAGGG CATCGTCTAT AATTATAGCT AAAAATTGTA TTTTAATTTG TATTTTTTGT   841 AATTTTAATT TCTCATTGTT TTACTTAAAA TGAATGCGAA TTAGAGAAAA ACTTTCACGA   901 ATGTCAAAAT AAGCTTCAGC AAAAATATAA CAATTTTAGG AAAGGCGATC AGCCACTTAT   961 TCTCGGTGGT AGATTCCTTT TCCTCCATAA TTCGAACAAT GTACTGGCCG ATGGATTTTC 1021 TCTTAGAATG CCTAATAATT GGTACTATGA TGATAGCCAG TTGATAG //
Primer: f1ori_R3 Sequence file: RDB14529_A6L7c.seq
>06199_14529_3_A6L7_f1ori_R3_A05_01_ABI08.ab1     1 GCCGATGGGT GTCCTTTGCG TATGCGGTGT GAATACCGCA CAGATGCGTA AGGAGAAAAT    61 ACCGCATCAG GCGCCATTCG CCATTCAGGC TGCGCAACTG TTGGGAAGGG CGATCGGTGC   121 GGGCCTCTTC GCTATTACGC CAGCTGGCGA AAGGGGGATG TGCTGCAAGG CGATTAAGTT   181 GGGTAACGCC AGGGTTTTCC CAGTCACGAC GTTGTAAAAC GACGGCCAGT GAATTCGAGC   241 TCGGGAGCGC TACCGTGAAT GGGCTCAACC AAACAATGAA CCTTTTCTTC TGATTTTCCT   301 ACCACACCGG AAAGGGAGGC AGAAGGCAAA AGGCCCAAGC TACCAGGAAT GACAGAAGCC   361 TCATCTGAAA TAATGTCACC AAACAAGTTG TCAGTCAAAA CAACACCGTT AAGTGTACGA   421 GGGCTCTTGA CCAAAAGCAT GGCTGCGGAG TCAATGAGCT GGTTTTTTAA GGTAAGGTGA   481 GGATATTCCT CCTTAAAAAT CTTAGCTACA GTCTTGCGCC AAAGACGAGA AGTTGCCAAA   541 ACATTAGCTT TGTCGAGTAA TGTGACGGGA GCAGGAGGGT TGGAAGTTTC AGCTAAGCGG   601 CCGCGGGCCC GAGTCTAACT CCTTAACCAC TCGGACATAG TGACTTATCT GACACTCATT   661 GTAAATTAAT ATATATATAG GCATTTTGTT TAGTTAAAGG TACTTAAGTA ATTAGTATAA   721 ACGAACCAAT TTTATAATCA GGAAGTTAAG TGAATGGTAG CACATGTCGT AAAAATTGTG   781 AATTTTTATT GAATAATATT TTAAATACAA GCCTTTCTAG ACTAGGTATA CTCATAAACA   841 TATGAGCAAA AGGATAGAGG AGATTACATT GCATCTTCTA CAAATTTATT TATTGCCCTT   901 TACTGAAAAA TTAAATAATG AGTACTAAAT GATAAAAAAG CGCTCAGTAC AAGAAAGATT   961 GCAAAAATAT TGCATTCTTT CATGAATTAA GTTGGCATAT AAGCATAATT GAAGGTTAAT  1021 AGTTACTAAA ACAGCAGTTA GCCGAAATAT AGGATTAATA TTCGCAGAAA TTGTGCACAT 1081 AAGTTGAAGG AAATGTGTAC GA //
Primer: Reverse2 Sequence file: RDB14529_A6L7d.seq
>06199_14529_3_A6L7_Reverse2_B05_02_ABI08.ab1     1 AGGGCCATGC ATTCGCCAAG CTTGTGATAT TGACGAAACT TTTTGACATC TAATTTATTC    61 TGTTCCAACA CCAATGTTTA TAACCAAGTT TTATCTTGTT TGTCTACATG GTATTTTACA   121 TTCATCTACA TACATCTTTC ATTGGCTTTG TACATAGTTA TCATTACAAG TCTAAAAAAA   181 TTCACTCTTT TCTTATTCAA TGTCAATCCA AGAGAAAAGA TTGTGGTAAT GTTGTAGGAG   241 CATGTTTAAT AAATTACTAT AGCAAATTAC TTTTTATTCC CAAGGTGTTT ATCTATAATA   301 GTTAATATTT TAGTCGCTAC ATAAAATTTT ACCAAAGAGT ACTTGTATAC TAATTCTAAA   361 TGCCTTCTGA CATAAAACGC CTAGGAAAAC AAACGCAAAC AAGGCATCGA CTTTTTCAAT   421 AACCAACCAA AAAAATTTTA CATTAGTCTT TTTTTAATGC TGAGAAAGTC TTTGCTGATA   481 TGCCTTCCAA CCAGCTTCTC TATATCTCTT GGCTTCGACA ACAGGATTAC GACCAGCTCC   541 ATAGACTCCA CGACCAACAA TGATGATGTC GCTACCGCAG TTTACAATCA CTTCTTCAGG   601 AGTACGATAT TGCTGTCCCA GCCCGTCTCC TTTAACATCC AAGCCGATAC CAGGGGACAT   661 AGTTATGTAG TCGCTTTGAA GGTTAGGAAA TCGACGACCA GCTATAAAGC CAAAGCAAAA   721 ATCGGTATGC TTCTCAAACC ATTCTAAGGT TTTCTCTGTG TAGGAACCAG TAGCCAAAGA   781 GCCTTTGGAA GACATTTCAG CCAAAAGCAA GAGACCACGT CCCAAAGGTA AACCAACTTC   841 TTTGAGGCCT TGTATAATAC CCTCGCCTGG CACTGTATGG CAATTTGTGA TATGAGCCCA   901 AGAAGCAATT TTGTACACAC CAGATGCATA TTGTAGCTTT GACGTATTTT CCCAATGTCT   961 GCGAATTTGG CGATCCTCAA GATAAGAAAC GATGCTTTTT TACCTAAGGC AACAAGTTTT  1021 TCTACCATAT TCCTTGTTCG GAATCCTCGA CCAACCGTCG ATATGGTCGT GATTAGCCAG 1081 GAGTATGGTT CCGT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.18

GNP_filter3_RDBDEP_html_220516.pl