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pDUAL2

S. pombe pDUAL2 series backbone vector

Catalog number RDB06192
Resource name pDUAL2
Clone info. S. pombe pDUAL2 series backbone vector
Multicopy marker, ura4+; Integration marker, ura4+, leu1-. Gateway incompatible.
Vector backbone pUC119 (Plasmid)
Selectable markers Amp^r
Reference of nucleotide sequence AB364058.1 (DDBJ accession number)
Depositor Yoshida, Minoru | Matsuyama, Akihisa |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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RDB06192 pDUAL2 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB06192z.seq from Depositor

Featured content

Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

References

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13002_A4Js.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: ADH-Forward RDB13002_A4Jsa.seq
>06192_13002_A4Js_ADH-Foward_D03_12.ab1
    1 TTATTCCTCT TATTGACACA CCTCTACCGG CAGATCCGCT AGGGATAACA GGGTAATATA
   61 CTAGTGCCTC TATTGATGCC TATGGAACCC CTTTGACTGA CGAGACTGTG AAGGCTTGTT
  121 TGGAAGCTGA CGGTGTTCTT TTGGGTGCCG TTGGTGGTCC TGAATGGACC AACCCCAATT
  181 GTCGTCCTGA GCAAGGTTTA TTGAAGCTTC GTAAGAGTAT GGGTGTTTGG GCCAACCTTC
  241 GACCTTGCAA CTTTGCCAGC AAGTCTTTAG TCAAGTACAG CCCTTTGAAG CCTGAAATCG
  301 TTGAAGGTGT CGATTTTTGT GTTGTACGAG AACTTACTGG AGGTTGTTAC TTTGGTGAGC
  361 GCACTGAGGA CAACGGATCG GGTTATGCTA TGGACACTTG GCCTTACAGT TTGGAAGAAG
  421 TTTCTCGTAT TGCTCGTTTG GCGGCCGCGG GCCCAACCTT CCAATTCATT AAATCGAGTC
  481 CTCCATCAGA TGATTTACAA GGTTTTGCAT AGAATCCCTA AGTAAGAGAA GTTGTTAGTG
  541 ATTTCTTCAA GTTTACTAAA ATTTACATAC AAATGGATGA TCTCTTCTCC ATTGTTTCCT
  601 TTCTTCTTGC AAACGCGTTT TACAAAGAGA TGACATGCTA TTTTCTGATT ATTTTTTTCT
  661 ATAGTTTTCT ATTTTGTACG TCCTTGGGAG TCCGAAATGT AAAATCGGGG TATGGTTAGT
  721 AGCGGCAGCG TTCATTATGG TATATAAAAG ATAAAAAGTA GGGGCAAGCG AGACAGAATG
  781 GGATACAAGG GCATCGTCTA TAATTATAGC TAAAAATTGT ATTTTAATTT GTATTTTTTG
  841 TAATTTTATT TCTCATTGTT TTACTTAAAA TGAATGCGAA TTAGAGAAAA CTTCAACGAA
  901 ATGTCAAAAT AAGCTCAGCA AAATATACAA TTTTAGGGAA AGCGATCAGC AACTTATTCT
  961 CCGTTGTAGA TTCTTTTTCT TCATAAATTC CAACAATGTA ACTGCCAATT TGATTTTTCT
 1021 CTAGATGCTA ATAAATTGAA ACTAATGAAA TATAATACAC AAGGTTTTGA ATAAATTGTA
 1081 AACTCTTACT TCAAAAAAAG ATTGTAAAAC TAATTAACTT TATAAGCTCA ATGGCCGTAA
1141 AGAGAATAGT AAAAAGCTCA TACCAAACTT T
//
Primer: M13_-40 RDB13002_A4Jsb.seq
>06192_13002_A4Js_M13(-40)_E03_15.ab1
    1 AGGCCGGTTA TTCGAGCTCG GGAGCGCTAC CGTGAATGGG CTCAACCAAA CAATGAACCT
   61 TTTCTTCTGA TTTTCCTACC ACACCGGAAA GGGAGGCAGA AGGCAAAAGG CCCAAGCTAC
  121 CAGGAATGAC AGAAGCCTCA TCTGAAATAA TGTCACCAAA CAAGTTGTCA GTCAAAACAA
  181 CACCGTTAAG TGTACGAGGG CTCTTGACCA AAAGCATGGC TGCGGAGTCA ATGAGCTGGT
  241 TTTTTAAGGT AAGGTGAGGA TATTCCTCCT TAAAAATCTT AGCTACAGTC TTGCGCCAAA
  301 GACGAGAAGT TGCCAAAACA TTAGCTTTGT CGAGTAATGT GACGGGAGCA GGAGGGTTGG
  361 AAGTTTCAGC TAAGCGGCCG CGGGCCCGAG TCTAACTCCT TAACCACTCG GACATAGTGA
  421 CTTATCTGAC ACTCATTGTA AATTAATATA TATATAGGCA TTTTGTTTAG TTAAAGGTAC
  481 TTAAGTAATT AGTATAAACG AACCAATTTT ATAATCAGGA AGTTAAGTGA ATGGTAGCAC
  541 ATGTCGTAAA AATTGTGAAT TTTTATTGAA TAATATTTTA AATACAAGCC TTTCTAGACT
  601 AGGTATACTC ATAAACATAT GAGCAAAAGG ATAGAGGAGA TTACATTGCA TCTTCTACAA
  661 ATTTATTTAT TGCCCTTTAC TGAAAAATTA AATAATGAGT ACTAAATGAT AAAAAGCGCT
  721 CAGTACAAGA AAGATTGCAA AAATATTGCA TTCTTCATGA ATTAAAGTTG CATATAAGGC
  781 ATATTGAAAG TAATAGTACT AAAACAGCAG TTAGCGAAAA TTAATAGAAT TATATTCGCA
  841 AGACAATTGT GACATTAAAT TAAAAAATTG TAGAATTTTT ACTATCCTCT TTAACGCCAT
  901 GAGCCTTTAT AAAAAGGTTA AATTAGTTTT AACATTCTTT TTTTGAGTAA GAGTTTACAA
  961 TTTATCAAAA CCTGTGTTAT TATATTCATT AGTTTCAATT TATTAGCATC TAGAGAAAAA
 1021 TCAAATTGGC AGTTACATTG TTGGAAATTT ATGAAGAAAA GAATCTACAC GGGAGAATAA
 1081 GTTGCTGATC GCTTTTCCTA AAATGTATTA TTTTTGCTGA GCTTATTTGA CATTCCGATG
1141 GAGTTTTCTT CTATCGCATT CATTTTAGGT TAAAAAACCA ATGGGAGCAA AAATCA
//
Primer: ADHrev RDB13002_A4Jsc.seq
>06192_13002_A4Js_ADHrev_F03_18.ab1
    1 GGGAAGGAAT TTTTCAAGAA ATAAGATTTT CGTTTTAAAA CCTAAGAGTT CACTTTAAAA
   61 TTTGTATACA CTTATTTTTT TTATAACTTA TTTAATAATA AAAATCATAA ATCATAAGAA
  121 ATTCGCTTAT TTAGAAGTGG CGCGCCGGAT CCTCTAGAGT CGACCTGCAG GCATGCAAGC
  181 TTAGCTACAA ATCCCACTGG CTATATGTAT GCATTTGTGT TAAAAAAGTT TGTATAGATT
  241 ATTTAATCTA CTCAGCATTC TTTCTCTAAA TAGGAATTTG TTACTTAATG GAGAAAAAAA
  301 TGTTTCGATT TACCTAGTGT ATTTGTTTGT ATACTCACGT TTAATTTCAA ACATCCATTC
  361 TATCTTGTGT AATTTTTGGC ATGGTGAAAA AGATAATCAG CCTTATAATC TTTACAAAAG
  421 TAAGAAATTC TGTAAATAAG CCTTAATGCC CTTGCTTTAA ATTAAAATGG TTCTTTTTCA
  481 TGATAATGTT TGCACTTTGT GAATATATTT TAGATAGTTC TGTGAGGTAT AATTAAGATG
  541 TTTTAGAGAC TTATACAATT TTGTCTTTAT AAATTCTTAA TTGATTTTAC CATCCCAGTT
  601 TAACTATGCT TCGTCGGCAT CTCTGCACAT GTCGTGTTTT CTTACCGTAT TGTCCTACCA
  661 AGAACCTCTT TTTTGCTTGG ATCGAAATTA AAGGTTTAAA AGCAAAGTTA TGGATGCTAG
  721 AGTATTTCAA AGCTATTCAG CTAGAGCTGA GGGGATGAAA AATCCCATTG CCAAGGAATT
  781 GTTGGCTTTG ATGGAAGAAA AGCAAAGCAA CTTGTCAGTC GCGGTCGATT TGACGAAGAA
  841 ATCCGAAATC TTAGAATTGG TAGATAAAAT TGGACCCTAT GTCTGTGTTA TCAAGACACA
  901 TATTGACGTT TGTCGAGGAT TTCGACCAGG ATATGGTAGA AAAACTGGTG GGCCTTAGGT
  961 AAAAAGCATC GTTTTCTTAT CTTTGAGGAT CGCAAATTTC GCAGACATTG GGAAATACCG
 1021 TCAAGCTACA ATATGCATCT GGTGTGTACA AAATTGCTTC TTGGGCTCAT ATCACAATTG
 1081 CCATACAGGT GCCAGGCGAG TATAATACAA GGGCCTCAAG AGTGTTAACT TGGGACGTGT
1141 CTTCTTGCCT TTTGGGCCTG AAGTGTCCTT TTC
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

2018.12.10

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