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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pDUAL2

S. pombe pDUAL2 series backbone vector

Catalog number RDB06192
Resource name pDUAL2
Clone info. S. pombe pDUAL2 series backbone vector
Comment Multicopy marker, ura4+; Integration marker, ura4+, leu1-. Gateway incompatible.
Vector backbone pUC119 (Plasmid)
Selectable markers Amp^r
Reference of insert sequence AB364058.1 (DDBJ accession number)
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、 寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
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            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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            RDB06192 pDUAL2 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB06192z.seq

            Original reference

            original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB13002_A4Js.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: ADH-Forward
            Sequence file: RDB13002_A4Jsa.seq check
            >06192_13002_A4Js_ADH-Foward_D03_12.ab1
                1 TTATTCCTCT TATTGACACA CCTCTACCGG CAGATCCGCT AGGGATAACA GGGTAATATA
               61 CTAGTGCCTC TATTGATGCC TATGGAACCC CTTTGACTGA CGAGACTGTG AAGGCTTGTT
              121 TGGAAGCTGA CGGTGTTCTT TTGGGTGCCG TTGGTGGTCC TGAATGGACC AACCCCAATT
              181 GTCGTCCTGA GCAAGGTTTA TTGAAGCTTC GTAAGAGTAT GGGTGTTTGG GCCAACCTTC
              241 GACCTTGCAA CTTTGCCAGC AAGTCTTTAG TCAAGTACAG CCCTTTGAAG CCTGAAATCG
              301 TTGAAGGTGT CGATTTTTGT GTTGTACGAG AACTTACTGG AGGTTGTTAC TTTGGTGAGC
              361 GCACTGAGGA CAACGGATCG GGTTATGCTA TGGACACTTG GCCTTACAGT TTGGAAGAAG
              421 TTTCTCGTAT TGCTCGTTTG GCGGCCGCGG GCCCAACCTT CCAATTCATT AAATCGAGTC
              481 CTCCATCAGA TGATTTACAA GGTTTTGCAT AGAATCCCTA AGTAAGAGAA GTTGTTAGTG
              541 ATTTCTTCAA GTTTACTAAA ATTTACATAC AAATGGATGA TCTCTTCTCC ATTGTTTCCT
              601 TTCTTCTTGC AAACGCGTTT TACAAAGAGA TGACATGCTA TTTTCTGATT ATTTTTTTCT
              661 ATAGTTTTCT ATTTTGTACG TCCTTGGGAG TCCGAAATGT AAAATCGGGG TATGGTTAGT
              721 AGCGGCAGCG TTCATTATGG TATATAAAAG ATAAAAAGTA GGGGCAAGCG AGACAGAATG
              781 GGATACAAGG GCATCGTCTA TAATTATAGC TAAAAATTGT ATTTTAATTT GTATTTTTTG
              841 TAATTTTATT TCTCATTGTT TTACTTAAAA TGAATGCGAA TTAGAGAAAA CTTCAACGAA
              901 ATGTCAAAAT AAGCTCAGCA AAATATACAA TTTTAGGGAA AGCGATCAGC AACTTATTCT
              961 CCGTTGTAGA TTCTTTTTCT TCATAAATTC CAACAATGTA ACTGCCAATT TGATTTTTCT
             1021 CTAGATGCTA ATAAATTGAA ACTAATGAAA TATAATACAC AAGGTTTTGA ATAAATTGTA
             1081 AACTCTTACT TCAAAAAAAG ATTGTAAAAC TAATTAACTT TATAAGCTCA ATGGCCGTAA
            1141 AGAGAATAGT AAAAAGCTCA TACCAAACTT T
            //
            Primer: M13_-40
            Sequence file: RDB13002_A4Jsb.seq check
            >06192_13002_A4Js_M13(-40)_E03_15.ab1
                1 AGGCCGGTTA TTCGAGCTCG GGAGCGCTAC CGTGAATGGG CTCAACCAAA CAATGAACCT
               61 TTTCTTCTGA TTTTCCTACC ACACCGGAAA GGGAGGCAGA AGGCAAAAGG CCCAAGCTAC
              121 CAGGAATGAC AGAAGCCTCA TCTGAAATAA TGTCACCAAA CAAGTTGTCA GTCAAAACAA
              181 CACCGTTAAG TGTACGAGGG CTCTTGACCA AAAGCATGGC TGCGGAGTCA ATGAGCTGGT
              241 TTTTTAAGGT AAGGTGAGGA TATTCCTCCT TAAAAATCTT AGCTACAGTC TTGCGCCAAA
              301 GACGAGAAGT TGCCAAAACA TTAGCTTTGT CGAGTAATGT GACGGGAGCA GGAGGGTTGG
              361 AAGTTTCAGC TAAGCGGCCG CGGGCCCGAG TCTAACTCCT TAACCACTCG GACATAGTGA
              421 CTTATCTGAC ACTCATTGTA AATTAATATA TATATAGGCA TTTTGTTTAG TTAAAGGTAC
              481 TTAAGTAATT AGTATAAACG AACCAATTTT ATAATCAGGA AGTTAAGTGA ATGGTAGCAC
              541 ATGTCGTAAA AATTGTGAAT TTTTATTGAA TAATATTTTA AATACAAGCC TTTCTAGACT
              601 AGGTATACTC ATAAACATAT GAGCAAAAGG ATAGAGGAGA TTACATTGCA TCTTCTACAA
              661 ATTTATTTAT TGCCCTTTAC TGAAAAATTA AATAATGAGT ACTAAATGAT AAAAAGCGCT
              721 CAGTACAAGA AAGATTGCAA AAATATTGCA TTCTTCATGA ATTAAAGTTG CATATAAGGC
              781 ATATTGAAAG TAATAGTACT AAAACAGCAG TTAGCGAAAA TTAATAGAAT TATATTCGCA
              841 AGACAATTGT GACATTAAAT TAAAAAATTG TAGAATTTTT ACTATCCTCT TTAACGCCAT
              901 GAGCCTTTAT AAAAAGGTTA AATTAGTTTT AACATTCTTT TTTTGAGTAA GAGTTTACAA
              961 TTTATCAAAA CCTGTGTTAT TATATTCATT AGTTTCAATT TATTAGCATC TAGAGAAAAA
             1021 TCAAATTGGC AGTTACATTG TTGGAAATTT ATGAAGAAAA GAATCTACAC GGGAGAATAA
             1081 GTTGCTGATC GCTTTTCCTA AAATGTATTA TTTTTGCTGA GCTTATTTGA CATTCCGATG
            1141 GAGTTTTCTT CTATCGCATT CATTTTAGGT TAAAAAACCA ATGGGAGCAA AAATCA
            //
            Primer: ADHrev
            Sequence file: RDB13002_A4Jsc.seq check
            >06192_13002_A4Js_ADHrev_F03_18.ab1
                1 GGGAAGGAAT TTTTCAAGAA ATAAGATTTT CGTTTTAAAA CCTAAGAGTT CACTTTAAAA
               61 TTTGTATACA CTTATTTTTT TTATAACTTA TTTAATAATA AAAATCATAA ATCATAAGAA
              121 ATTCGCTTAT TTAGAAGTGG CGCGCCGGAT CCTCTAGAGT CGACCTGCAG GCATGCAAGC
              181 TTAGCTACAA ATCCCACTGG CTATATGTAT GCATTTGTGT TAAAAAAGTT TGTATAGATT
              241 ATTTAATCTA CTCAGCATTC TTTCTCTAAA TAGGAATTTG TTACTTAATG GAGAAAAAAA
              301 TGTTTCGATT TACCTAGTGT ATTTGTTTGT ATACTCACGT TTAATTTCAA ACATCCATTC
              361 TATCTTGTGT AATTTTTGGC ATGGTGAAAA AGATAATCAG CCTTATAATC TTTACAAAAG
              421 TAAGAAATTC TGTAAATAAG CCTTAATGCC CTTGCTTTAA ATTAAAATGG TTCTTTTTCA
              481 TGATAATGTT TGCACTTTGT GAATATATTT TAGATAGTTC TGTGAGGTAT AATTAAGATG
              541 TTTTAGAGAC TTATACAATT TTGTCTTTAT AAATTCTTAA TTGATTTTAC CATCCCAGTT
              601 TAACTATGCT TCGTCGGCAT CTCTGCACAT GTCGTGTTTT CTTACCGTAT TGTCCTACCA
              661 AGAACCTCTT TTTTGCTTGG ATCGAAATTA AAGGTTTAAA AGCAAAGTTA TGGATGCTAG
              721 AGTATTTCAA AGCTATTCAG CTAGAGCTGA GGGGATGAAA AATCCCATTG CCAAGGAATT
              781 GTTGGCTTTG ATGGAAGAAA AGCAAAGCAA CTTGTCAGTC GCGGTCGATT TGACGAAGAA
              841 ATCCGAAATC TTAGAATTGG TAGATAAAAT TGGACCCTAT GTCTGTGTTA TCAAGACACA
              901 TATTGACGTT TGTCGAGGAT TTCGACCAGG ATATGGTAGA AAAACTGGTG GGCCTTAGGT
              961 AAAAAGCATC GTTTTCTTAT CTTTGAGGAT CGCAAATTTC GCAGACATTG GGAAATACCG
             1021 TCAAGCTACA ATATGCATCT GGTGTGTACA AAATTGCTTC TTGGGCTCAT ATCACAATTG
             1081 CCATACAGGT GCCAGGCGAG TATAATACAA GGGCCTCAAG AGTGTTAACT TGGGACGTGT
            1141 CTTCTTGCCT TTTGGGCCTG AAGTGTCCTT TTC
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

            2022.05.18

            GNP_filter3_RDBDEP_html_220516.pl