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pDUAL2 (#RDB06192)

S. pombe pDUAL2 series backbone vector

Clone info. S. pombe pDUAL2 series backbone vector
Comment Multicopy marker, ura4+; Integration marker, ura4+, leu1-. Gateway incompatible.
Vector backbone pUC119 (Plasmid)
Selectable markers Amp^r
Reference of insert sequence AB364058.1 (DDBJ accession number)
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
 
Sequence RDB06192z.seq

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、 寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。

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RDB06192 pDUAL2 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pDUAL2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06192).

Reference section:

Matsuyama, A., Yoshida, M., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913. [link to RRC of NBRP]
Matsuyama, A., Shirai, A., Yashiroda, Y., Kamata, A., Horinouchi, S., Yoshida, M., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13002_A4Js.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: ADH-Forward
Sequence file: RDB13002_A4Jsa.seq check
>06192_13002_A4Js_ADH-Foward_D03_12.ab1
    1 TTATTCCTCT TATTGACACA CCTCTACCGG CAGATCCGCT AGGGATAACA GGGTAATATA
   61 CTAGTGCCTC TATTGATGCC TATGGAACCC CTTTGACTGA CGAGACTGTG AAGGCTTGTT
  121 TGGAAGCTGA CGGTGTTCTT TTGGGTGCCG TTGGTGGTCC TGAATGGACC AACCCCAATT
  181 GTCGTCCTGA GCAAGGTTTA TTGAAGCTTC GTAAGAGTAT GGGTGTTTGG GCCAACCTTC
  241 GACCTTGCAA CTTTGCCAGC AAGTCTTTAG TCAAGTACAG CCCTTTGAAG CCTGAAATCG
  301 TTGAAGGTGT CGATTTTTGT GTTGTACGAG AACTTACTGG AGGTTGTTAC TTTGGTGAGC
  361 GCACTGAGGA CAACGGATCG GGTTATGCTA TGGACACTTG GCCTTACAGT TTGGAAGAAG
  421 TTTCTCGTAT TGCTCGTTTG GCGGCCGCGG GCCCAACCTT CCAATTCATT AAATCGAGTC
  481 CTCCATCAGA TGATTTACAA GGTTTTGCAT AGAATCCCTA AGTAAGAGAA GTTGTTAGTG
  541 ATTTCTTCAA GTTTACTAAA ATTTACATAC AAATGGATGA TCTCTTCTCC ATTGTTTCCT
  601 TTCTTCTTGC AAACGCGTTT TACAAAGAGA TGACATGCTA TTTTCTGATT ATTTTTTTCT
  661 ATAGTTTTCT ATTTTGTACG TCCTTGGGAG TCCGAAATGT AAAATCGGGG TATGGTTAGT
  721 AGCGGCAGCG TTCATTATGG TATATAAAAG ATAAAAAGTA GGGGCAAGCG AGACAGAATG
  781 GGATACAAGG GCATCGTCTA TAATTATAGC TAAAAATTGT ATTTTAATTT GTATTTTTTG
  841 TAATTTTATT TCTCATTGTT TTACTTAAAA TGAATGCGAA TTAGAGAAAA CTTCAACGAA
  901 ATGTCAAAAT AAGCTCAGCA AAATATACAA TTTTAGGGAA AGCGATCAGC AACTTATTCT
  961 CCGTTGTAGA TTCTTTTTCT TCATAAATTC CAACAATGTA ACTGCCAATT TGATTTTTCT
 1021 CTAGATGCTA ATAAATTGAA ACTAATGAAA TATAATACAC AAGGTTTTGA ATAAATTGTA
 1081 AACTCTTACT TCAAAAAAAG ATTGTAAAAC TAATTAACTT TATAAGCTCA ATGGCCGTAA
1141 AGAGAATAGT AAAAAGCTCA TACCAAACTT T
//
Primer: M13_-40
Sequence file: RDB13002_A4Jsb.seq check
>06192_13002_A4Js_M13(-40)_E03_15.ab1
    1 AGGCCGGTTA TTCGAGCTCG GGAGCGCTAC CGTGAATGGG CTCAACCAAA CAATGAACCT
   61 TTTCTTCTGA TTTTCCTACC ACACCGGAAA GGGAGGCAGA AGGCAAAAGG CCCAAGCTAC
  121 CAGGAATGAC AGAAGCCTCA TCTGAAATAA TGTCACCAAA CAAGTTGTCA GTCAAAACAA
  181 CACCGTTAAG TGTACGAGGG CTCTTGACCA AAAGCATGGC TGCGGAGTCA ATGAGCTGGT
  241 TTTTTAAGGT AAGGTGAGGA TATTCCTCCT TAAAAATCTT AGCTACAGTC TTGCGCCAAA
  301 GACGAGAAGT TGCCAAAACA TTAGCTTTGT CGAGTAATGT GACGGGAGCA GGAGGGTTGG
  361 AAGTTTCAGC TAAGCGGCCG CGGGCCCGAG TCTAACTCCT TAACCACTCG GACATAGTGA
  421 CTTATCTGAC ACTCATTGTA AATTAATATA TATATAGGCA TTTTGTTTAG TTAAAGGTAC
  481 TTAAGTAATT AGTATAAACG AACCAATTTT ATAATCAGGA AGTTAAGTGA ATGGTAGCAC
  541 ATGTCGTAAA AATTGTGAAT TTTTATTGAA TAATATTTTA AATACAAGCC TTTCTAGACT
  601 AGGTATACTC ATAAACATAT GAGCAAAAGG ATAGAGGAGA TTACATTGCA TCTTCTACAA
  661 ATTTATTTAT TGCCCTTTAC TGAAAAATTA AATAATGAGT ACTAAATGAT AAAAAGCGCT
  721 CAGTACAAGA AAGATTGCAA AAATATTGCA TTCTTCATGA ATTAAAGTTG CATATAAGGC
  781 ATATTGAAAG TAATAGTACT AAAACAGCAG TTAGCGAAAA TTAATAGAAT TATATTCGCA
  841 AGACAATTGT GACATTAAAT TAAAAAATTG TAGAATTTTT ACTATCCTCT TTAACGCCAT
  901 GAGCCTTTAT AAAAAGGTTA AATTAGTTTT AACATTCTTT TTTTGAGTAA GAGTTTACAA
  961 TTTATCAAAA CCTGTGTTAT TATATTCATT AGTTTCAATT TATTAGCATC TAGAGAAAAA
 1021 TCAAATTGGC AGTTACATTG TTGGAAATTT ATGAAGAAAA GAATCTACAC GGGAGAATAA
 1081 GTTGCTGATC GCTTTTCCTA AAATGTATTA TTTTTGCTGA GCTTATTTGA CATTCCGATG
1141 GAGTTTTCTT CTATCGCATT CATTTTAGGT TAAAAAACCA ATGGGAGCAA AAATCA
//
Primer: ADHrev
Sequence file: RDB13002_A4Jsc.seq check
>06192_13002_A4Js_ADHrev_F03_18.ab1
    1 GGGAAGGAAT TTTTCAAGAA ATAAGATTTT CGTTTTAAAA CCTAAGAGTT CACTTTAAAA
   61 TTTGTATACA CTTATTTTTT TTATAACTTA TTTAATAATA AAAATCATAA ATCATAAGAA
  121 ATTCGCTTAT TTAGAAGTGG CGCGCCGGAT CCTCTAGAGT CGACCTGCAG GCATGCAAGC
  181 TTAGCTACAA ATCCCACTGG CTATATGTAT GCATTTGTGT TAAAAAAGTT TGTATAGATT
  241 ATTTAATCTA CTCAGCATTC TTTCTCTAAA TAGGAATTTG TTACTTAATG GAGAAAAAAA
  301 TGTTTCGATT TACCTAGTGT ATTTGTTTGT ATACTCACGT TTAATTTCAA ACATCCATTC
  361 TATCTTGTGT AATTTTTGGC ATGGTGAAAA AGATAATCAG CCTTATAATC TTTACAAAAG
  421 TAAGAAATTC TGTAAATAAG CCTTAATGCC CTTGCTTTAA ATTAAAATGG TTCTTTTTCA
  481 TGATAATGTT TGCACTTTGT GAATATATTT TAGATAGTTC TGTGAGGTAT AATTAAGATG
  541 TTTTAGAGAC TTATACAATT TTGTCTTTAT AAATTCTTAA TTGATTTTAC CATCCCAGTT
  601 TAACTATGCT TCGTCGGCAT CTCTGCACAT GTCGTGTTTT CTTACCGTAT TGTCCTACCA
  661 AGAACCTCTT TTTTGCTTGG ATCGAAATTA AAGGTTTAAA AGCAAAGTTA TGGATGCTAG
  721 AGTATTTCAA AGCTATTCAG CTAGAGCTGA GGGGATGAAA AATCCCATTG CCAAGGAATT
  781 GTTGGCTTTG ATGGAAGAAA AGCAAAGCAA CTTGTCAGTC GCGGTCGATT TGACGAAGAA
  841 ATCCGAAATC TTAGAATTGG TAGATAAAAT TGGACCCTAT GTCTGTGTTA TCAAGACACA
  901 TATTGACGTT TGTCGAGGAT TTCGACCAGG ATATGGTAGA AAAACTGGTG GGCCTTAGGT
  961 AAAAAGCATC GTTTTCTTAT CTTTGAGGAT CGCAAATTTC GCAGACATTG GGAAATACCG
 1021 TCAAGCTACA ATATGCATCT GGTGTGTACA AAATTGCTTC TTGGGCTCAT ATCACAATTG
 1081 CCATACAGGT GCCAGGCGAG TATAATACAA GGGCCTCAAG AGTGTTAACT TGGGACGTGT
1141 CTTCTTGCCT TTTGGGCCTG AAGTGTCCTT TTC
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913. [link to RRC of NBRP]
original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. [link to RRC of NBRP]