Please review the QC test results indicated by icon below as well as clone information before placing your order.

pDUAL-HFF1

S. pombe 5 ter end His6-FLAG2-tagged vector with nmt1 promoter

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.

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Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、　寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

References and tips

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB12776_A4Dpp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: ADH_rev Sequence file: RDB12776_A4Dpa.seq
>06179_12776_A4Dp_ADHrev_A01_01.ab1     1 TGGGGCTCTC AGATAAGATT TTCGTTTTAA ACCTAAGAGT CACTTTAAAA TTTGTATACA    61 CTTATTTTTT TTATAACTTA TTTAATAATA AAAATCATAA ATCATAAGAA ATTCGCTTAT   121 TTAGAAGTGG CGCGCCGGAT CCTCTAGAGT CGAGATCTCA TGATATCTTT GTCATCGTCG   181 TCCTTGTAGT CCATGGGTTT ATCGTCATCA TCCTTATAAT CCATATGATG ATGGTGGTGA   241 TGCATAGGAT CCAGGCCTGT CGACGCTAGC TTTAACAAAG CGACTATAAG TCAGAAAGTG   301 AGAATGAGAT TGAAATAATT AATTCAACTT ATTCAATTGA TGATATGCCA GGATTCCTCT   361 TCCTTTATAT ATCTTCAATG TTTCGATTAA CAGGTCCATT ATCCGGTTTT TAGTTTCGCT   421 GCACATTGCC GAATGACAAT CGGATTCCTT TAAGCTTTGC TTTTGCCCTG TTTTCTCTCC   481 CCAGTAATCG TTGGGTGACT GAACCATCCA GTAGCCGAGA GTTACGTTTT TCCTGATTTA   541 ATAAATATCC ATATCTATTT GTCTCGTGGT GTTTTACAGC AACTAACCGA AATTACTATT   601 TTGCATCATC CAACCATAAA GAGACAACAA TAATTCTAGA AAACATATAA CAAGTGTTTT   661 TTTCTATTTA CGTCTCATAC GCAAAGTCCA CTTTACCATG TTTTGGAAAC TTGTACCCAA   721 CCATTCATTT TTCGATTTTG TAATGAGGCT CTTTTCTTCT AACAATCATT GAGGTCATTA   781 TCATGTGTCC TCACCTATTG TTTAAATTGA GGCTTGCGCT ATTCCCAAAA TTGTGGGTAT   841 TATTTTTCAT ATATCAGTAT CTGCAATTTC GGTTCAACTT TCTTATCAAG AAAGAGGATT   901 AATTTCTTAA CAAAAACCTT CTTTGTTTTA TAACGCTGAT TTTTTGTATT ATTTGGCGAC   961 CAAAGGTTTA TAAGTGATCC CAAATCCCAA ATGTCATAAA CTTCAGTTTG ATCCCTGCGG  1021 AAAATATAAA TCAGAATTAA TGGGTTATTA TAAAAAAAGA GAATTATTTG AAGTTCAATT 1081 GCCAAACTTT TCGAG //
Primer: ADH-Forward Sequence file: RDB12776_A4Dpc.seq
>06179_12776_A4Dp_ADH-Forward_B01_04.ab1     1 AATGTCCTTA ATTGAACACA CCTCTACCGG CAGATCCGCT AGGGATAACA GGGTAATATA    61 CTAGTAAGAT GATTCTTTTA TCGTCGATAA CGATGAGGAT GTAGACGATT ATGATGAGAG   121 TGATTAGCTT CTAAAATTTG GTGTTTTCAA ACGCTATTTA CAGTACAATC TGAAAAAATT   181 GGATGAACAT TGTAAATGGT AGGATACGAG TTCCAATTAA TGTATTTTTT AAGTATTACA   241 CTTAGCTTAT TAATTTTATA TATACATTCT ACCTCTGTGG GTTTAAAGAC AACTCGTACA   301 CATACTCAAA TTATTTATGA ACATTATAAT GCATATTCGT ACTGTCTATT AAACATTTTT   361 TTGACATTAA AAATTTCGTT TACTAACGTA GAAAGCGCTG ATAATTGAGG CTACTATTTT   421 AATATCTAGA AACAAAGATT CATTAATTAA TTAAGTAAGC TCCAGGATAC TTGTATATTT   481 CGTTAAAAAA GCGCAATTTC AACAATTCCT ATGAACATCC ATGATTTCAT CACAAAATCA   541 AACATATCAG GATTTAACGT GAATTATTTG CTTTCTTGTG ATTTTCATCT GATTTCTGGT   601 CATTTACGTT ACTGTAAACA AGCTTTTTAA AAAAAGGATT TTTTACGCAA TATTATCGAT   661 ATCCCAATCT GTAGTATAAA AAGTGCAACA GTGTTGAGTT TCCCGAAACC AGGCATTTTT   721 GCGGTAGGGT CTGAAACTTT CACGAAACCA TTTCACAACA AAACTTCTTC AACAACATGT   781 GTGCAAAGAA AATCGTCGTC TTACCAGGAG ACCATATTGG CCCTGAAATT GTTGCTTCTG   841 CCTTGGAGGT TTTGAAAGTC GTTGAGAAGA AGCGACCTGA GTTAAAACTC GAGTTTGAAG   901 AACACAAGAT TGGAGGTGCC TCTATTGATG CCTATGGAAC CCCTTTGACT GACGAGACTG   961 TGAAGGCTTG TTTGGAAGCT GACGGTGTTC TTTTGGGTGC CGTTGGTGGT CCTGAATGGA  1021 CCAACCCCAA CGTTCGTCCT GAGCAGGTTA ATGCAGCCTC CGTAGAGTAT GCTGTTGGCC 1081 AACGATCGAA CCTTGCAACC TTTTGGCCAG AC //
Primer: ars_R1 Sequence file: RDB12776_A4Dpd.seq
>06179_12776_A4Dp_ars_R1_C01_07.ab1     1 TGAATCGATT TATCTAATTA CTTAAGTACC TTTAACTAAA CAAAATGCCT ATATATATAT    61 TAATTTACAA TGAGTGTCAG ATAAGTCACT ATGTCCGAGT GGTTAAGGAG TTAGACTCGA   121 ATTCGCAAGC TTAGCTACAA ATCCCACTGG CTATATGTAT GCATTTGTGT TAAAAAAGTT   181 TGTATAGATT ATTTAATCTA CTCAGCATTC TTTCTCTAAA TAGGAATTTG TTACTTAATG   241 GAGAAAAAAA TGTTTCGATT TACCTAGTGT ATTTGTTTGT ATACTCACGT TTAATTTCAA   301 ACATCCATTC TATCTTGTGT AATTTTTGGC ATGGTGAAAA AGATAATCAG CCTTATAATC   361 TTTACAAAAG TAAGAAATTC TGTAAATAAG CCTTAATGCC CTTGCTTTAA ATTAAAATGG   421 TTCTTTTTCA TGATAATGTT TGCACTTTGT GAATATATTT TAGATAGTTC TGTGAGGTAT   481 AATTAAGATG TTTTAGAGAC TTATACAATT TTGTCTTTAT AAATTCTTAA TTGATTTTAC   541 CATCCCAGTT TAACTATGCT TCGTCGGCAT CTCTGCACAT GTCGTGTTTT CTTACCGTAT   601 TGTCCTACCA AGAACCTCTT TTTTGCTTGG ATCGAAATTA AAGGTTTAAA AGCAAAGTTA   661 TGGATGCTAG AGTATTTCAA AGCTATTCAG CTAGAGCTGA GGGGATGAAA AATCCCATTG   721 CCAAGGAATT GTTGGCTTTG ATGGAAGAAA GCAAAGCAAC TTGTCAGTCG CGGTCGATTT   781 GACGAAGAAA TCCGAAATCT TAGAATTGGT AGATAAAATT GGACCCTATG TCTGTGTTAT   841 CAAGACACAT ATTGACGTTG TCGAGGATTT CGACCAGGAT ATGGTAGAAA AACTGGTGGC   901 CTTAGGTAAA AAGCATCGTT TTCTTATCTT TGAGGATCGC AAATTCGCAG ACATTGGAAA   961 TACCGTCAAG CTACAATATG CATCTGGTGT GTACAAAAAT TGCTTCTTGG GGCTCATATC  1021 ACAAATTGGC CATACAGTGC CCAGGCGAGG TATTATACAG GTCCTCAAAG AAGTGGATTA 1081 ACGTTTGGGA CGTGGTCTTC TTGCCTTTTT GGG //
Primer: f1ori_R3 Sequence file: RDB12776_A4Dpe.seq
>06179_12776_A4Dp_f1ori_R3_D01_10.ab1     1 GCCATCGCAT GCGTTGCGGT GTGAATACCG CACAGATGCG TAAGGAGAAA ATACCGCATC    61 AGGCGCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG GGCGATCGGT GCGGGCCTCT   121 TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA GGCGATTAAG TTGGGTAACG   181 CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA GTGAATTCGA GCTCGGGAGC   241 GCTACCGTGA ATGGGCTCAA CCAAACAATG AACCTTTTCT TCTGATTTTC CTACCACACC   301 GGAAAGGGAG GCAGAAGGCA AAAGGCCCAA GCTACCAGGA ATGACAGAAG CCTCATCTGA   361 AATAATGTCA CCAAACAAGT TGTCAGTCAA AACAACACCG TTAAGTGTAC GAGGGCTCTT   421 GACCAAAAGC ATGGCTGCGG AGTCAATGAG CTGGTTTTTT AAGGTAAGGT GAGGATATTC   481 CTCCTTAAAA ATCTTAGCTA CAGTCTTGCG CCAAAGACGA GAAGTTGCCA AAACATTAGC   541 TTTGTCGAGT AATGTGACGG GAGCAGGAGG GTTGGAAGTT TCAGCTAAGC GGCCGCGGGC   601 CCAACCTTCC AATTCATTAA ATCGAGTCCT CCATCAGATG ATTTACAAGG TTTTGCATAG   661 AATCCCTAAG TAAGAGAAGT TGTTAGTGAT TTCTTCAAGT TTACTAAAAT TTACATACAA   721 ATGGATGATC TCTTCTCCAT TGTTTCCTTT CTTCTTGCAA ACGCGTTTTA CAAAGAGATG   781 ACATGCTATT TTCTGATTAT TTTTTTCTAT AGTTTTCTAT TTTGTACGTC CTTGGGAGTC   841 CGAAATGTAA AATCGGGGTA TGGTTAGTAG CGGCAGCGTT CATTATGGTA TATAAAAGAT   901 AAAAAAGTAC GGGCAAGCGA GACAGAATGG GATACAAGGG CATCGTCTAT AATTATAGCT   961 AAAAAATTGT ATTTTTAAAT TTGTATTTTT TTGTAAATTT TTATTTCTCA TTGTTTTTAC  1021 TTTAAAATGA AATGCGAAAT TAGAGAAAAC CTTTCCAACC GAAATGTCAA AATTAAGGCT 1081 CAGCCAAAAT TATACGAATT TAACGGGAAG CAATCAGCAA GCGTTAACTT CCG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.02

GNP_filter3_RDBDEP_html_230501.pl