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pDUAL-HFF1

S. pombe 5 ter end His6-FLAG2-tagged vector with nmt1 promoter

Catalog number RDB06179
Resource name pDUAL-HFF1
Clone info. S. pombe 5 ter end His6-FLAG2-tagged vector with nmt1 promoter.
Multicopy marker, ura4+; Integration marker, leu1+. Gateway incompatible.
Vector backbone pDUAL (Plasmid)
Selectable markers Amp^r
Reference of nucleotide sequence AB364046.1 (DDBJ accession number)
Depositor Yoshida, Minoru | Matsuyama, Akihisa |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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RDB06179 pDUAL-HFF1 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB06179z.seq from Depositor

Featured content

Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

References

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12776_A4Dpp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: ADH_rev RDB12776_A4Dpa.seq
>06179_12776_A4Dp_ADHrev_A01_01.ab1
    1 TGGGGCTCTC AGATAAGATT TTCGTTTTAA ACCTAAGAGT CACTTTAAAA TTTGTATACA
   61 CTTATTTTTT TTATAACTTA TTTAATAATA AAAATCATAA ATCATAAGAA ATTCGCTTAT
  121 TTAGAAGTGG CGCGCCGGAT CCTCTAGAGT CGAGATCTCA TGATATCTTT GTCATCGTCG
  181 TCCTTGTAGT CCATGGGTTT ATCGTCATCA TCCTTATAAT CCATATGATG ATGGTGGTGA
  241 TGCATAGGAT CCAGGCCTGT CGACGCTAGC TTTAACAAAG CGACTATAAG TCAGAAAGTG
  301 AGAATGAGAT TGAAATAATT AATTCAACTT ATTCAATTGA TGATATGCCA GGATTCCTCT
  361 TCCTTTATAT ATCTTCAATG TTTCGATTAA CAGGTCCATT ATCCGGTTTT TAGTTTCGCT
  421 GCACATTGCC GAATGACAAT CGGATTCCTT TAAGCTTTGC TTTTGCCCTG TTTTCTCTCC
  481 CCAGTAATCG TTGGGTGACT GAACCATCCA GTAGCCGAGA GTTACGTTTT TCCTGATTTA
  541 ATAAATATCC ATATCTATTT GTCTCGTGGT GTTTTACAGC AACTAACCGA AATTACTATT
  601 TTGCATCATC CAACCATAAA GAGACAACAA TAATTCTAGA AAACATATAA CAAGTGTTTT
  661 TTTCTATTTA CGTCTCATAC GCAAAGTCCA CTTTACCATG TTTTGGAAAC TTGTACCCAA
  721 CCATTCATTT TTCGATTTTG TAATGAGGCT CTTTTCTTCT AACAATCATT GAGGTCATTA
  781 TCATGTGTCC TCACCTATTG TTTAAATTGA GGCTTGCGCT ATTCCCAAAA TTGTGGGTAT
  841 TATTTTTCAT ATATCAGTAT CTGCAATTTC GGTTCAACTT TCTTATCAAG AAAGAGGATT
  901 AATTTCTTAA CAAAAACCTT CTTTGTTTTA TAACGCTGAT TTTTTGTATT ATTTGGCGAC
  961 CAAAGGTTTA TAAGTGATCC CAAATCCCAA ATGTCATAAA CTTCAGTTTG ATCCCTGCGG
 1021 AAAATATAAA TCAGAATTAA TGGGTTATTA TAAAAAAAGA GAATTATTTG AAGTTCAATT
1081 GCCAAACTTT TCGAG
//
Primer: ADH-Forward RDB12776_A4Dpc.seq
>06179_12776_A4Dp_ADH-Forward_B01_04.ab1
    1 AATGTCCTTA ATTGAACACA CCTCTACCGG CAGATCCGCT AGGGATAACA GGGTAATATA
   61 CTAGTAAGAT GATTCTTTTA TCGTCGATAA CGATGAGGAT GTAGACGATT ATGATGAGAG
  121 TGATTAGCTT CTAAAATTTG GTGTTTTCAA ACGCTATTTA CAGTACAATC TGAAAAAATT
  181 GGATGAACAT TGTAAATGGT AGGATACGAG TTCCAATTAA TGTATTTTTT AAGTATTACA
  241 CTTAGCTTAT TAATTTTATA TATACATTCT ACCTCTGTGG GTTTAAAGAC AACTCGTACA
  301 CATACTCAAA TTATTTATGA ACATTATAAT GCATATTCGT ACTGTCTATT AAACATTTTT
  361 TTGACATTAA AAATTTCGTT TACTAACGTA GAAAGCGCTG ATAATTGAGG CTACTATTTT
  421 AATATCTAGA AACAAAGATT CATTAATTAA TTAAGTAAGC TCCAGGATAC TTGTATATTT
  481 CGTTAAAAAA GCGCAATTTC AACAATTCCT ATGAACATCC ATGATTTCAT CACAAAATCA
  541 AACATATCAG GATTTAACGT GAATTATTTG CTTTCTTGTG ATTTTCATCT GATTTCTGGT
  601 CATTTACGTT ACTGTAAACA AGCTTTTTAA AAAAAGGATT TTTTACGCAA TATTATCGAT
  661 ATCCCAATCT GTAGTATAAA AAGTGCAACA GTGTTGAGTT TCCCGAAACC AGGCATTTTT
  721 GCGGTAGGGT CTGAAACTTT CACGAAACCA TTTCACAACA AAACTTCTTC AACAACATGT
  781 GTGCAAAGAA AATCGTCGTC TTACCAGGAG ACCATATTGG CCCTGAAATT GTTGCTTCTG
  841 CCTTGGAGGT TTTGAAAGTC GTTGAGAAGA AGCGACCTGA GTTAAAACTC GAGTTTGAAG
  901 AACACAAGAT TGGAGGTGCC TCTATTGATG CCTATGGAAC CCCTTTGACT GACGAGACTG
  961 TGAAGGCTTG TTTGGAAGCT GACGGTGTTC TTTTGGGTGC CGTTGGTGGT CCTGAATGGA
 1021 CCAACCCCAA CGTTCGTCCT GAGCAGGTTA ATGCAGCCTC CGTAGAGTAT GCTGTTGGCC
1081 AACGATCGAA CCTTGCAACC TTTTGGCCAG AC
//
Primer: ars_R1 RDB12776_A4Dpd.seq
>06179_12776_A4Dp_ars_R1_C01_07.ab1
    1 TGAATCGATT TATCTAATTA CTTAAGTACC TTTAACTAAA CAAAATGCCT ATATATATAT
   61 TAATTTACAA TGAGTGTCAG ATAAGTCACT ATGTCCGAGT GGTTAAGGAG TTAGACTCGA
  121 ATTCGCAAGC TTAGCTACAA ATCCCACTGG CTATATGTAT GCATTTGTGT TAAAAAAGTT
  181 TGTATAGATT ATTTAATCTA CTCAGCATTC TTTCTCTAAA TAGGAATTTG TTACTTAATG
  241 GAGAAAAAAA TGTTTCGATT TACCTAGTGT ATTTGTTTGT ATACTCACGT TTAATTTCAA
  301 ACATCCATTC TATCTTGTGT AATTTTTGGC ATGGTGAAAA AGATAATCAG CCTTATAATC
  361 TTTACAAAAG TAAGAAATTC TGTAAATAAG CCTTAATGCC CTTGCTTTAA ATTAAAATGG
  421 TTCTTTTTCA TGATAATGTT TGCACTTTGT GAATATATTT TAGATAGTTC TGTGAGGTAT
  481 AATTAAGATG TTTTAGAGAC TTATACAATT TTGTCTTTAT AAATTCTTAA TTGATTTTAC
  541 CATCCCAGTT TAACTATGCT TCGTCGGCAT CTCTGCACAT GTCGTGTTTT CTTACCGTAT
  601 TGTCCTACCA AGAACCTCTT TTTTGCTTGG ATCGAAATTA AAGGTTTAAA AGCAAAGTTA
  661 TGGATGCTAG AGTATTTCAA AGCTATTCAG CTAGAGCTGA GGGGATGAAA AATCCCATTG
  721 CCAAGGAATT GTTGGCTTTG ATGGAAGAAA GCAAAGCAAC TTGTCAGTCG CGGTCGATTT
  781 GACGAAGAAA TCCGAAATCT TAGAATTGGT AGATAAAATT GGACCCTATG TCTGTGTTAT
  841 CAAGACACAT ATTGACGTTG TCGAGGATTT CGACCAGGAT ATGGTAGAAA AACTGGTGGC
  901 CTTAGGTAAA AAGCATCGTT TTCTTATCTT TGAGGATCGC AAATTCGCAG ACATTGGAAA
  961 TACCGTCAAG CTACAATATG CATCTGGTGT GTACAAAAAT TGCTTCTTGG GGCTCATATC
 1021 ACAAATTGGC CATACAGTGC CCAGGCGAGG TATTATACAG GTCCTCAAAG AAGTGGATTA
1081 ACGTTTGGGA CGTGGTCTTC TTGCCTTTTT GGG
//
Primer: f1ori_R3 RDB12776_A4Dpe.seq
>06179_12776_A4Dp_f1ori_R3_D01_10.ab1
    1 GCCATCGCAT GCGTTGCGGT GTGAATACCG CACAGATGCG TAAGGAGAAA ATACCGCATC
   61 AGGCGCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG GGCGATCGGT GCGGGCCTCT
  121 TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA GGCGATTAAG TTGGGTAACG
  181 CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA GTGAATTCGA GCTCGGGAGC
  241 GCTACCGTGA ATGGGCTCAA CCAAACAATG AACCTTTTCT TCTGATTTTC CTACCACACC
  301 GGAAAGGGAG GCAGAAGGCA AAAGGCCCAA GCTACCAGGA ATGACAGAAG CCTCATCTGA
  361 AATAATGTCA CCAAACAAGT TGTCAGTCAA AACAACACCG TTAAGTGTAC GAGGGCTCTT
  421 GACCAAAAGC ATGGCTGCGG AGTCAATGAG CTGGTTTTTT AAGGTAAGGT GAGGATATTC
  481 CTCCTTAAAA ATCTTAGCTA CAGTCTTGCG CCAAAGACGA GAAGTTGCCA AAACATTAGC
  541 TTTGTCGAGT AATGTGACGG GAGCAGGAGG GTTGGAAGTT TCAGCTAAGC GGCCGCGGGC
  601 CCAACCTTCC AATTCATTAA ATCGAGTCCT CCATCAGATG ATTTACAAGG TTTTGCATAG
  661 AATCCCTAAG TAAGAGAAGT TGTTAGTGAT TTCTTCAAGT TTACTAAAAT TTACATACAA
  721 ATGGATGATC TCTTCTCCAT TGTTTCCTTT CTTCTTGCAA ACGCGTTTTA CAAAGAGATG
  781 ACATGCTATT TTCTGATTAT TTTTTTCTAT AGTTTTCTAT TTTGTACGTC CTTGGGAGTC
  841 CGAAATGTAA AATCGGGGTA TGGTTAGTAG CGGCAGCGTT CATTATGGTA TATAAAAGAT
  901 AAAAAAGTAC GGGCAAGCGA GACAGAATGG GATACAAGGG CATCGTCTAT AATTATAGCT
  961 AAAAAATTGT ATTTTTAAAT TTGTATTTTT TTGTAAATTT TTATTTCTCA TTGTTTTTAC
 1021 TTTAAAATGA AATGCGAAAT TAGAGAAAAC CTTTCCAACC GAAATGTCAA AATTAAGGCT
1081 CAGCCAAAAT TATACGAATT TAACGGGAAG CAATCAGCAA GCGTTAACTT CCG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.
user report Kanda, K., Application of a phosphite dehydrogenase gene as a novel dominant selection marker for yeasts. J. Biotechnol. 182-183: 68-73 (2014). PMID 24786825.

2018.12.10

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