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pDUAL-HFF1

S. pombe 5 ter end His6-FLAG2-tagged vector with nmt1 promoter

Catalog number RDB06179
Resource name pDUAL-HFF1
Clone info. S. pombe 5 ter end His6-FLAG2-tagged vector with nmt1 promoter.
Comment Multicopy marker, ura4+; Integration marker, leu1+. Gateway incompatible.
Vector backbone pDUAL (Plasmid)
Selectable markers Amp^r
Reference of insert sequence AB364046.1 (DDBJ accession number)
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、 寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
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            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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            RDB06179 pDUAL-HFF1 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB06179z.seq

            Original reference

            original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB12776_A4Dpp1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: ADH_rev
            Sequence file: RDB12776_A4Dpa.seq check
            >06179_12776_A4Dp_ADHrev_A01_01.ab1
                1 TGGGGCTCTC AGATAAGATT TTCGTTTTAA ACCTAAGAGT CACTTTAAAA TTTGTATACA
               61 CTTATTTTTT TTATAACTTA TTTAATAATA AAAATCATAA ATCATAAGAA ATTCGCTTAT
              121 TTAGAAGTGG CGCGCCGGAT CCTCTAGAGT CGAGATCTCA TGATATCTTT GTCATCGTCG
              181 TCCTTGTAGT CCATGGGTTT ATCGTCATCA TCCTTATAAT CCATATGATG ATGGTGGTGA
              241 TGCATAGGAT CCAGGCCTGT CGACGCTAGC TTTAACAAAG CGACTATAAG TCAGAAAGTG
              301 AGAATGAGAT TGAAATAATT AATTCAACTT ATTCAATTGA TGATATGCCA GGATTCCTCT
              361 TCCTTTATAT ATCTTCAATG TTTCGATTAA CAGGTCCATT ATCCGGTTTT TAGTTTCGCT
              421 GCACATTGCC GAATGACAAT CGGATTCCTT TAAGCTTTGC TTTTGCCCTG TTTTCTCTCC
              481 CCAGTAATCG TTGGGTGACT GAACCATCCA GTAGCCGAGA GTTACGTTTT TCCTGATTTA
              541 ATAAATATCC ATATCTATTT GTCTCGTGGT GTTTTACAGC AACTAACCGA AATTACTATT
              601 TTGCATCATC CAACCATAAA GAGACAACAA TAATTCTAGA AAACATATAA CAAGTGTTTT
              661 TTTCTATTTA CGTCTCATAC GCAAAGTCCA CTTTACCATG TTTTGGAAAC TTGTACCCAA
              721 CCATTCATTT TTCGATTTTG TAATGAGGCT CTTTTCTTCT AACAATCATT GAGGTCATTA
              781 TCATGTGTCC TCACCTATTG TTTAAATTGA GGCTTGCGCT ATTCCCAAAA TTGTGGGTAT
              841 TATTTTTCAT ATATCAGTAT CTGCAATTTC GGTTCAACTT TCTTATCAAG AAAGAGGATT
              901 AATTTCTTAA CAAAAACCTT CTTTGTTTTA TAACGCTGAT TTTTTGTATT ATTTGGCGAC
              961 CAAAGGTTTA TAAGTGATCC CAAATCCCAA ATGTCATAAA CTTCAGTTTG ATCCCTGCGG
             1021 AAAATATAAA TCAGAATTAA TGGGTTATTA TAAAAAAAGA GAATTATTTG AAGTTCAATT
            1081 GCCAAACTTT TCGAG
            //
            Primer: ADH-Forward
            Sequence file: RDB12776_A4Dpc.seq check
            >06179_12776_A4Dp_ADH-Forward_B01_04.ab1
                1 AATGTCCTTA ATTGAACACA CCTCTACCGG CAGATCCGCT AGGGATAACA GGGTAATATA
               61 CTAGTAAGAT GATTCTTTTA TCGTCGATAA CGATGAGGAT GTAGACGATT ATGATGAGAG
              121 TGATTAGCTT CTAAAATTTG GTGTTTTCAA ACGCTATTTA CAGTACAATC TGAAAAAATT
              181 GGATGAACAT TGTAAATGGT AGGATACGAG TTCCAATTAA TGTATTTTTT AAGTATTACA
              241 CTTAGCTTAT TAATTTTATA TATACATTCT ACCTCTGTGG GTTTAAAGAC AACTCGTACA
              301 CATACTCAAA TTATTTATGA ACATTATAAT GCATATTCGT ACTGTCTATT AAACATTTTT
              361 TTGACATTAA AAATTTCGTT TACTAACGTA GAAAGCGCTG ATAATTGAGG CTACTATTTT
              421 AATATCTAGA AACAAAGATT CATTAATTAA TTAAGTAAGC TCCAGGATAC TTGTATATTT
              481 CGTTAAAAAA GCGCAATTTC AACAATTCCT ATGAACATCC ATGATTTCAT CACAAAATCA
              541 AACATATCAG GATTTAACGT GAATTATTTG CTTTCTTGTG ATTTTCATCT GATTTCTGGT
              601 CATTTACGTT ACTGTAAACA AGCTTTTTAA AAAAAGGATT TTTTACGCAA TATTATCGAT
              661 ATCCCAATCT GTAGTATAAA AAGTGCAACA GTGTTGAGTT TCCCGAAACC AGGCATTTTT
              721 GCGGTAGGGT CTGAAACTTT CACGAAACCA TTTCACAACA AAACTTCTTC AACAACATGT
              781 GTGCAAAGAA AATCGTCGTC TTACCAGGAG ACCATATTGG CCCTGAAATT GTTGCTTCTG
              841 CCTTGGAGGT TTTGAAAGTC GTTGAGAAGA AGCGACCTGA GTTAAAACTC GAGTTTGAAG
              901 AACACAAGAT TGGAGGTGCC TCTATTGATG CCTATGGAAC CCCTTTGACT GACGAGACTG
              961 TGAAGGCTTG TTTGGAAGCT GACGGTGTTC TTTTGGGTGC CGTTGGTGGT CCTGAATGGA
             1021 CCAACCCCAA CGTTCGTCCT GAGCAGGTTA ATGCAGCCTC CGTAGAGTAT GCTGTTGGCC
            1081 AACGATCGAA CCTTGCAACC TTTTGGCCAG AC
            //
            Primer: ars_R1
            Sequence file: RDB12776_A4Dpd.seq check
            >06179_12776_A4Dp_ars_R1_C01_07.ab1
                1 TGAATCGATT TATCTAATTA CTTAAGTACC TTTAACTAAA CAAAATGCCT ATATATATAT
               61 TAATTTACAA TGAGTGTCAG ATAAGTCACT ATGTCCGAGT GGTTAAGGAG TTAGACTCGA
              121 ATTCGCAAGC TTAGCTACAA ATCCCACTGG CTATATGTAT GCATTTGTGT TAAAAAAGTT
              181 TGTATAGATT ATTTAATCTA CTCAGCATTC TTTCTCTAAA TAGGAATTTG TTACTTAATG
              241 GAGAAAAAAA TGTTTCGATT TACCTAGTGT ATTTGTTTGT ATACTCACGT TTAATTTCAA
              301 ACATCCATTC TATCTTGTGT AATTTTTGGC ATGGTGAAAA AGATAATCAG CCTTATAATC
              361 TTTACAAAAG TAAGAAATTC TGTAAATAAG CCTTAATGCC CTTGCTTTAA ATTAAAATGG
              421 TTCTTTTTCA TGATAATGTT TGCACTTTGT GAATATATTT TAGATAGTTC TGTGAGGTAT
              481 AATTAAGATG TTTTAGAGAC TTATACAATT TTGTCTTTAT AAATTCTTAA TTGATTTTAC
              541 CATCCCAGTT TAACTATGCT TCGTCGGCAT CTCTGCACAT GTCGTGTTTT CTTACCGTAT
              601 TGTCCTACCA AGAACCTCTT TTTTGCTTGG ATCGAAATTA AAGGTTTAAA AGCAAAGTTA
              661 TGGATGCTAG AGTATTTCAA AGCTATTCAG CTAGAGCTGA GGGGATGAAA AATCCCATTG
              721 CCAAGGAATT GTTGGCTTTG ATGGAAGAAA GCAAAGCAAC TTGTCAGTCG CGGTCGATTT
              781 GACGAAGAAA TCCGAAATCT TAGAATTGGT AGATAAAATT GGACCCTATG TCTGTGTTAT
              841 CAAGACACAT ATTGACGTTG TCGAGGATTT CGACCAGGAT ATGGTAGAAA AACTGGTGGC
              901 CTTAGGTAAA AAGCATCGTT TTCTTATCTT TGAGGATCGC AAATTCGCAG ACATTGGAAA
              961 TACCGTCAAG CTACAATATG CATCTGGTGT GTACAAAAAT TGCTTCTTGG GGCTCATATC
             1021 ACAAATTGGC CATACAGTGC CCAGGCGAGG TATTATACAG GTCCTCAAAG AAGTGGATTA
            1081 ACGTTTGGGA CGTGGTCTTC TTGCCTTTTT GGG
            //
            Primer: f1ori_R3
            Sequence file: RDB12776_A4Dpe.seq check
            >06179_12776_A4Dp_f1ori_R3_D01_10.ab1
                1 GCCATCGCAT GCGTTGCGGT GTGAATACCG CACAGATGCG TAAGGAGAAA ATACCGCATC
               61 AGGCGCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG GGCGATCGGT GCGGGCCTCT
              121 TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA GGCGATTAAG TTGGGTAACG
              181 CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA GTGAATTCGA GCTCGGGAGC
              241 GCTACCGTGA ATGGGCTCAA CCAAACAATG AACCTTTTCT TCTGATTTTC CTACCACACC
              301 GGAAAGGGAG GCAGAAGGCA AAAGGCCCAA GCTACCAGGA ATGACAGAAG CCTCATCTGA
              361 AATAATGTCA CCAAACAAGT TGTCAGTCAA AACAACACCG TTAAGTGTAC GAGGGCTCTT
              421 GACCAAAAGC ATGGCTGCGG AGTCAATGAG CTGGTTTTTT AAGGTAAGGT GAGGATATTC
              481 CTCCTTAAAA ATCTTAGCTA CAGTCTTGCG CCAAAGACGA GAAGTTGCCA AAACATTAGC
              541 TTTGTCGAGT AATGTGACGG GAGCAGGAGG GTTGGAAGTT TCAGCTAAGC GGCCGCGGGC
              601 CCAACCTTCC AATTCATTAA ATCGAGTCCT CCATCAGATG ATTTACAAGG TTTTGCATAG
              661 AATCCCTAAG TAAGAGAAGT TGTTAGTGAT TTCTTCAAGT TTACTAAAAT TTACATACAA
              721 ATGGATGATC TCTTCTCCAT TGTTTCCTTT CTTCTTGCAA ACGCGTTTTA CAAAGAGATG
              781 ACATGCTATT TTCTGATTAT TTTTTTCTAT AGTTTTCTAT TTTGTACGTC CTTGGGAGTC
              841 CGAAATGTAA AATCGGGGTA TGGTTAGTAG CGGCAGCGTT CATTATGGTA TATAAAAGAT
              901 AAAAAAGTAC GGGCAAGCGA GACAGAATGG GATACAAGGG CATCGTCTAT AATTATAGCT
              961 AAAAAATTGT ATTTTTAAAT TTGTATTTTT TTGTAAATTT TTATTTCTCA TTGTTTTTAC
             1021 TTTAAAATGA AATGCGAAAT TAGAGAAAAC CTTTCCAACC GAAATGTCAA AATTAAGGCT
            1081 CAGCCAAAAT TATACGAATT TAACGGGAAG CAATCAGCAA GCGTTAACTT CCG
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.
            user_report Zhang, N., Interfering with retrotransposition by two types of CRISPR effectors: Cas12a and Cas13a. Cell Discov. 6: 30 (2020). PMID 32435507.
            user_report Jing, X., Implementation of the CRISPR-Cas13a system in fission yeast and its repurposing for precise RNA editing. Nucleic Acids Res. 46 (15): e90 (2018). PMID 29860393.
            user_report Kanda, K., Application of a phosphite dehydrogenase gene as a novel dominant selection marker for yeasts. J. Biotechnol. 182-183: 68-73 (2014). PMID 24786825.

            2022.05.18

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