Sequencing and PCR primers for primer information.
S. pombe 5 ter end His6-FLAG2-tagged vector with nmt1 promoter
| Catalog number | RDB06154 |
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| Resource name | pDUAL2-HFF1c |
| Clone info. | S. pombe 5 ter end His6-FLAG2-tagged vector with nmt1 promoter. Multicopy marker, ura4+; Integration marker, ura4+, leu1-. Gateway compatible. |
| Vector backbone | pDUAL2 (Plasmid) |
| Selectable markers | E. coli: Amp^r, Cm^r, ccdB. |
| Growth remarks | Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine. |
| Reference of nucleotide sequence | AB364083.1 (DDBJ accession number) |
| Depositor | Yoshida, Minoru | Matsuyama, Akihisa | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation. |
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| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
| Remarks | Use Survival2 (Invitrogen) or equivalent to amplify this clone. |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB06154 | pDUAL2-HFF1c | DNA solution |
Electronic file
| Electronic file | Sequence RDB06154z.seq from Depositor |
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Original reference
| original | Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. |
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Further references such as user reports and related articles (go to bottom)
Featured content
| Featured content | Expression Vector Backbone of Schizosaccharomyces pombe (English text) |
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RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB13298_A5Df.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: nmt1Fw | Sequence: RDB13298_A5Dfa.seq |
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>06154_13298_A5Df_nmt1Fw_B07_04.ab1 1 GAATTTTAAT TAAATCAGGG AAAACGTAAC TCTCGGCTAC TGGATGGTTC AGTCACCCAA 61 CGATTACTGG GGAGAGAAAA CAGGGCAAAA GCAAAGCTTA AAGGAATCCG ATTGTCATTC 121 GGCAATGTGC AGCGAAACTA AAAACCGGAT AATGGACCTG TTAATCGAAA CATTGAAGAT 181 ATATAAAGGA AGAGGAATCC TGGCATATCA TCAATTGAAT AAGTTGAATT AATTATTTCA 241 ATCTCATTCT CACTTTCTGA CTTATAGTCG CTTTGTTAAA GCTAGCGTCG ACAGGCCTGG 301 ATCCTATGCA TCACCACCAT CATCATATGG ATTATAAGGA TGATGACGAT AAACCCATGG 361 ACTACAAGGA CGACGATGAC AAAGATATCA CAAGTTTGTA CAAAAAAGCT GAACGAGAAA 421 CGTAAAATGA TATAAATATC AATATATTAA ATTAGATTTT GCATAAAAAA CAGACTACAT 481 AATACTGTAA AACACAACAT ATCCAGTCAC TATGGCGGCC GCATTAGGCA CCCCAGGCTT 541 TACACTTTAT GCTTCCGGCT CGTATAATGT GTGGATTTTG AGTTAGGATC CGTCGAGATT 601 TTCAGGAGCT AAGGAAGCTA AAATGGAGAA AAAAATCACT GGATATACCA CCGTTGATAT 661 ATCCCAATGG CATCGTAAAG AACATTTTGA GGCATTTCAG TCAGTTGCTC AATGTACCTA 721 TAACCAGACC GTTCAGCTGG ATATTACGGC CTTTTTAAAG ACCGTAAAGA AAAATAAGCA 781 CAAGTTTTAT CCGGCCTTTA TTCACATTCT TGCCCGCCTG ATGAATGCTC ATCCGGAATT 841 CCGTATGGCA ATGAAAGACG GTGAGCTGGT GATATGGGAT AGTGTTCACC CTTGTTACAC 901 CGTTTTCCAT GAGCAAACTG AAACGTTTTC ATCGCTCTGG AGTGAATACC ACGACGATTT 961 CCGGCAGTTT CTACACATAT ATTCGCAAGA TGTGGCGTGT TACGGTGAAA ACCCTGGTCT 1021 ATTTCCTAAA GGGTTTATTG AGAATATGTT TTTCGTCTCA GCCAAATCCC TGCGTGAGTT 1081 TCACAGTTTT GAATTTAACG TGCAATATGG ACACTCGTCG GCCCCCGTAT TTCACATGAA 1141 CTACTATTAA TACGGCA // |
| Primer: ADH_rev | Sequence: RDB13298_A5Dfb.seq |
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>06154_13298_A5Df_ADH_rev_C07_07.ab1 1 TAGGATCTCA GATAGATTTT CGTTTTAAAC CTAAGAGTCA CTTTAAAATT TGTATACACT 61 TATTTTTTTT ATAACTTATT TAATAATAAA AATCATAAAT CATAAGAAAT TCGCTTATTT 121 AGAAGTGGCG CGCCGGATCC TCTAGAGTCG AGATCTCATG ATATCACCAC TTTGTACAAG 181 AAAGCTGAAC GAGAAACGTA AAATGATATA AATATCAATA TATTAAATTA GATTTTGCAT 241 AAAAAACAGA CTACATAATA CTGTAAAACA CAACATATCC AGTCACTATG GTCGACCTGC 301 AGACTGGCTG TGTATAAGGG AGCCTGACAT TTATATTCCC CAGAACATCA GGTTAATGGC 361 GTTTTTGATG TCATTTTCGC GGTGGCTGAG ATCAGCCACT TCTTCCCCGA TAACGGAGAC 421 CGGCACACTG GCCATATCGG TGGTCATCAT GCGCCAGCTT TCATCCCCGA TATGCACCAC 481 CGGGTAAAGT TCACGGGAGA CTTTATCTGA CAGCAGACGT GCACTGGCCA GGGGGATCAC 541 CATCCGTCGC CCGGGCGTGT CAATAATATC ACTCTGTACA TCCACAAACA GACGATAACG 601 GCTCTCTCTT TTATAGGTGT AAACCTTAAA CTGCATTTCA CCAGCCCCTG TTCTCGTCAG 661 CAAAAGAGCC GTTCATTTCA ATAAACCGGG CGACCTCAGC CATCCCTTCC TGATTTTCCG 721 CTTTCCAGCG TTCGGCACGC AGACGACGGG CTTCATTCTG CATGGTTGTG CTTACCAGAC 781 CGGAGATATT GACATCATAT ATGCCTTGAG CAACTGATAG CTGTCGCTGT CAACTGTCAC 841 TGTAATACGC TGCTTCATAG CATACCTCTT TTTGACATAC TTCGGGTATA CATATCAGTA 901 TATATTCTTA TACCGCAAAA ATCAGCGCGC AAATACGCAT ACTGTTATCT GGCTTTTAGT 961 AAGCCGGGAT CCACGCGTTT ACGCCCCGCC CTGCCACTCA TCGCAGTACT GTGTAATTCA 1021 TTTAAGCAAT TCTGGCGACA TGCAGCATCA CAAACGCAAT GATGGACTGG ATCGCAGCGG 1081 CATCAGCACT GGTCGCTTGC GTATATATTT TGCCCATGGG GTGAAC // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
2019.09.27
GNP_filter3_RDBDEP_html_190927.pl