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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pDUAL2-HFF1c

S. pombe 5 ter end His6-FLAG2-tagged vector with nmt1 promoter

Catalog number RDB06154
Resource name pDUAL2-HFF1c
Clone info. S. pombe 5 ter end His6-FLAG2-tagged vector with nmt1 promoter. Multicopy marker, ura4+; Integration marker, ura4+, leu1-.
Comment Gateway compatible.
Vector backbone pDUAL2 (Plasmid)
Selectable markers E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Reference of insert sequence AB364083.1 (DDBJ accession number)
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
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            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、　寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            備考 このクローンの増殖にはSurvival2 (Invitrogen) あるいは同等の宿主を使用してください。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB06154 pDUAL2-HFF1c DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB06154z.seq

            Original reference

            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB13298_A5Df.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: nmt1Fw
            Sequence file: RDB13298_A5Dfa.seq check
            >06154_13298_A5Df_nmt1Fw_B07_04.ab1
                1 GAATTTTAAT TAAATCAGGG AAAACGTAAC TCTCGGCTAC TGGATGGTTC AGTCACCCAA
               61 CGATTACTGG GGAGAGAAAA CAGGGCAAAA GCAAAGCTTA AAGGAATCCG ATTGTCATTC
              121 GGCAATGTGC AGCGAAACTA AAAACCGGAT AATGGACCTG TTAATCGAAA CATTGAAGAT
              181 ATATAAAGGA AGAGGAATCC TGGCATATCA TCAATTGAAT AAGTTGAATT AATTATTTCA
              241 ATCTCATTCT CACTTTCTGA CTTATAGTCG CTTTGTTAAA GCTAGCGTCG ACAGGCCTGG
              301 ATCCTATGCA TCACCACCAT CATCATATGG ATTATAAGGA TGATGACGAT AAACCCATGG
              361 ACTACAAGGA CGACGATGAC AAAGATATCA CAAGTTTGTA CAAAAAAGCT GAACGAGAAA
              421 CGTAAAATGA TATAAATATC AATATATTAA ATTAGATTTT GCATAAAAAA CAGACTACAT
              481 AATACTGTAA AACACAACAT ATCCAGTCAC TATGGCGGCC GCATTAGGCA CCCCAGGCTT
              541 TACACTTTAT GCTTCCGGCT CGTATAATGT GTGGATTTTG AGTTAGGATC CGTCGAGATT
              601 TTCAGGAGCT AAGGAAGCTA AAATGGAGAA AAAAATCACT GGATATACCA CCGTTGATAT
              661 ATCCCAATGG CATCGTAAAG AACATTTTGA GGCATTTCAG TCAGTTGCTC AATGTACCTA
              721 TAACCAGACC GTTCAGCTGG ATATTACGGC CTTTTTAAAG ACCGTAAAGA AAAATAAGCA
              781 CAAGTTTTAT CCGGCCTTTA TTCACATTCT TGCCCGCCTG ATGAATGCTC ATCCGGAATT
              841 CCGTATGGCA ATGAAAGACG GTGAGCTGGT GATATGGGAT AGTGTTCACC CTTGTTACAC
              901 CGTTTTCCAT GAGCAAACTG AAACGTTTTC ATCGCTCTGG AGTGAATACC ACGACGATTT
              961 CCGGCAGTTT CTACACATAT ATTCGCAAGA TGTGGCGTGT TACGGTGAAA ACCCTGGTCT
             1021 ATTTCCTAAA GGGTTTATTG AGAATATGTT TTTCGTCTCA GCCAAATCCC TGCGTGAGTT
             1081 TCACAGTTTT GAATTTAACG TGCAATATGG ACACTCGTCG GCCCCCGTAT TTCACATGAA
            1141 CTACTATTAA TACGGCA
            //
            Primer: ADH_rev
            Sequence file: RDB13298_A5Dfb.seq check
            >06154_13298_A5Df_ADH_rev_C07_07.ab1
                1 TAGGATCTCA GATAGATTTT CGTTTTAAAC CTAAGAGTCA CTTTAAAATT TGTATACACT
               61 TATTTTTTTT ATAACTTATT TAATAATAAA AATCATAAAT CATAAGAAAT TCGCTTATTT
              121 AGAAGTGGCG CGCCGGATCC TCTAGAGTCG AGATCTCATG ATATCACCAC TTTGTACAAG
              181 AAAGCTGAAC GAGAAACGTA AAATGATATA AATATCAATA TATTAAATTA GATTTTGCAT
              241 AAAAAACAGA CTACATAATA CTGTAAAACA CAACATATCC AGTCACTATG GTCGACCTGC
              301 AGACTGGCTG TGTATAAGGG AGCCTGACAT TTATATTCCC CAGAACATCA GGTTAATGGC
              361 GTTTTTGATG TCATTTTCGC GGTGGCTGAG ATCAGCCACT TCTTCCCCGA TAACGGAGAC
              421 CGGCACACTG GCCATATCGG TGGTCATCAT GCGCCAGCTT TCATCCCCGA TATGCACCAC
              481 CGGGTAAAGT TCACGGGAGA CTTTATCTGA CAGCAGACGT GCACTGGCCA GGGGGATCAC
              541 CATCCGTCGC CCGGGCGTGT CAATAATATC ACTCTGTACA TCCACAAACA GACGATAACG
              601 GCTCTCTCTT TTATAGGTGT AAACCTTAAA CTGCATTTCA CCAGCCCCTG TTCTCGTCAG
              661 CAAAAGAGCC GTTCATTTCA ATAAACCGGG CGACCTCAGC CATCCCTTCC TGATTTTCCG
              721 CTTTCCAGCG TTCGGCACGC AGACGACGGG CTTCATTCTG CATGGTTGTG CTTACCAGAC
              781 CGGAGATATT GACATCATAT ATGCCTTGAG CAACTGATAG CTGTCGCTGT CAACTGTCAC
              841 TGTAATACGC TGCTTCATAG CATACCTCTT TTTGACATAC TTCGGGTATA CATATCAGTA
              901 TATATTCTTA TACCGCAAAA ATCAGCGCGC AAATACGCAT ACTGTTATCT GGCTTTTAGT
              961 AAGCCGGGAT CCACGCGTTT ACGCCCCGCC CTGCCACTCA TCGCAGTACT GTGTAATTCA
             1021 TTTAAGCAAT TCTGGCGACA TGCAGCATCA CAAACGCAAT GATGGACTGG ATCGCAGCGG
            1081 CATCAGCACT GGTCGCTTGC GTATATATTT TGCCCATGGG GTGAAC
            //

            Please visit Sequencing and PCR primers for primer information.

            References

            Original, user report and related articles

            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.
            user_report Toteva, T., Establishment of expression-state boundaries by Rif1 and Taz1 in fission yeast. Proc. Natl. Acad. Sci. U.S.A. 114 (5): 1093-1098 (2017). PMID 28096402.

            2022.05.18

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