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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pDUAL-HFF1c

S. pombe 5 ter end His6-FLAG2-tagged vector with nmt1 promoter

Catalog number RDB06130
Resource name pDUAL-HFF1c
Clone info. S. pombe 5 ter end His6-FLAG2-tagged vector with nmt1 promoter. Multicopy marker, ura4+; Integration marker, leu1+.
Comment Gateway compatible.
Vector backbone pDUAL (Plasmid)
Selectable markers E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Reference of insert sequence AB364071.1 (DDBJ accession number)
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、 寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            備考 このクローンの増殖にはSurvival2 (Invitrogen) あるいは同等の宿主を使用してください。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB06130 pDUAL-HFF1c DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB06130z.seq
            Remarks, protocol and/or map (pdf) RDB06130.pdf

            Original reference

            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB12155_A3H8.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: nmt1Fw
            Sequence file: RDB12155_A3H8a.seq check
            >06130_12155_A3H8_nmt1Fw_A01_01.ab1
                1 AGAAGTTTTA ATTTAAATCA GGAAAACGTA ACTCTCGGCT ACTGGATGGT TCAGTCACCC
               61 AACGATTACT GGGGAGAGAA AACAGGGCAA AAGCAAAGCT TAAAGGAATC CGATTGTCAT
              121 TCGGCAATGT GCAGCGAAAC TAAAAACCGG ATAATGGACC TGTTAATCGA AACATTGAAG
              181 ATATATAAAG GAAGAGGAAT CCTGGCATAT CATCAATTGA ATAAGTTGAA TTAATTATTT
              241 CAATCTCATT CTCACTTTCT GACTTATAGT CGCTTTGTTA AAGCTAGCGT CGACAGGCCT
              301 GGATCCTATG CATCACCACC ATCATCATAT GGATTATAAG GATGATGACG ATAAACCCAT
              361 GGACTACAAG GACGACGATG ACAAAGATAT CACAAGTTTG TACAAAAAAG CTGAACGAGA
              421 AACGTAAAAT GATATAAATA TCAATATATT AAATTAGATT TTGCATAAAA AACAGACTAC
              481 ATAATACTGT AAAACACAAC ATATCCAGTC ACTATGGCGG CCGCATTAGG CACCCCAGGC
              541 TTTACACTTT ATGCTTCCGG CTCGTATAAT GTGTGGATTT TGAGTTAGGA TCCGTCGAGA
              601 TTTTCAGGAG CTAAGGAAGC TAAAATGGAG AAAAAAATCA CTGGATATAC CACCGTTGAT
              661 ATATCCCAAT GGCATCGTAA AGAACATTTT GAGGCATTTC AGTCAGTTGC TCAATGTACC
              721 TATAACCAGA CCGTTCAGCT GGATATTACG GCCTTTTTAA AGACCGTAAA GAAAAATAAG
              781 CACAAGTTTT ATCCGGCCTT TATTCACATT CTTGCCCGCC TGATGAATGC TCATCCGGAA
              841 TTCCGTATGG CAATGAAAGA CGGTGAGCTG GTGATATGGG ATAGTGTTCA CCCTTGTTAC
              901 ACCGTTTTCC ATGAGCAAAC TGAAACGTTT TCATCGCTCT GGAGTGAATA CCACGACGAT
              961 TTCCGGCAGT TTCTACACAT ATATTCGCAA GATGTGGCGT GTTACGGTGA AAACCTGGCC
             1021 CTATTTCCCT AAAGGGTTTA TTGAGATATG TTTTTCGTCT CAGCCAAATC CCTGGTGAGT
             1081 TTCACAGTTT TGATTTAAAC CGTGATCGAT ATGGACCAAC TCCATCGCCC CCGATTTTCA
            1141 CCATGGGGCC AAAATAATA
            //
            Primer: ADH_rev
            Sequence file: RDB12155_A3H8b.seq check
            >06130_12155_A3H8_ADHrev_B01_04.ab1
                1 GGAAGAGTCT CAGGATAAGA TTTTCGTTTT AAACCTAAGA GTCACTTTAA AATTTGTATA
               61 CACTTATTTT TTTTATAACT TATTTAATAA TAAAAATCAT AAATCATAAG AAATTCGCTT
              121 ATTTAGAAGT GGCGCGCCGG ATCCTCTAGA GTCGAGATCT CATGATATCA CCACTTTGTA
              181 CAAGAAAGCT GAACGAGAAA CGTAAAATGA TATAAATATC AATATATTAA ATTAGATTTT
              241 GCATAAAAAA CAGACTACAT AATACTGTAA AACACAACAT ATCCAGTCAC TATGGTCGAC
              301 CTGCAGACTG GCTGTGTATA AGGGAGCCTG ACATTTATAT TCCCCAGAAC ATCAGGTTAA
              361 TGGCGTTTTT GATGTCATTT TCGCGGTGGC TGAGATCAGC CACTTCTTCC CCGATAACGG
              421 AGACCGGCAC ACTGGCCATA TCGGTGGTCA TCATGCGCCA GCTTTCATCC CCGATATGCA
              481 CCACCGGGTA AAGTTCACGG GAGACTTTAT CTGACAGCAG ACGTGCACTG GCCAGGGGGA
              541 TCACCATCCG TCGCCCGGGC GTGTCAATAA TATCACTCTG TACATCCACA AACAGACGAT
              601 AACGGCTCTC TCTTTTATAG GTGTAAACCT TAAACTGCAT TTCACCAGCC CCTGTTCTCG
              661 TCAGCAAAAG AGCCGTTCAT TTCAATAAAC CGGGCGACCT CAGCCATCCC TTCCTGATTT
              721 TCCGCTTTCC AGCGTTCGGC ACGCAGACGA CGGGCTTCAT TCTGCATGGT TGTGCTTACC
              781 AGACCGGAGA TATTGACATC ATATATGCCT TGAGCAACTG ATAGCTGTCG CTGTCAACTG
              841 TCACTGTAAT ACGCTGCTTC ATAGCATACC TCTTTTTTGA CATACTTCGG GTATACATAT
              901 CAGTATATAT TCTTATACCG CAAAAATCAG CGCGCAAATA CGCATACTGT TATCTGGCTT
              961 TTAGTAAGCC GGGATCCACG CGTTTACGCC CCGCCCTGCC ACTCATCGCA GTACTGTTGT
             1021 AATTCATTAA GCATTCCTGC CGACATGGAA AGCCATCACA AACGGGCATG GATGATCTGG
             1081 AATCGGCAGG CGGCATCAGC ACTGATCGCC TTTGCGTATA AATATTTTGC CCCAATGGGG
            1141 TGAGA
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

            2022.05.18

            GNP_filter3_RDBDEP_html_220516.pl