Sequencing and PCR primers for primer information.
S. pombe 5 ter end His6-FLAG2-tagged vector with nmt1 promoter
| Catalog number | RDB06130 |
|---|---|
| Resource name | pDUAL-HFF1c |
| Clone info. | S. pombe 5 ter end His6-FLAG2-tagged vector with nmt1 promoter. Multicopy marker, ura4+; Integration marker, leu1+. Gateway compatible. |
| Vector backbone | pDUAL (Plasmid) |
| Selectable markers | E. coli: Amp^r, Cm^r, ccdB. |
| Growth remarks | Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine. |
| Reference of nucleotide sequence | AB364071.1 (DDBJ accession number) |
| Depositor | Yoshida, Minoru | Matsuyama, Akihisa | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation. |
|---|---|
| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
| Remarks | Use Survival2 (Invitrogen) or equivalent to amplify this clone. |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB06130 | pDUAL-HFF1c | DNA solution |
Electronic file
| Electronic file | Sequence RDB06130z.seq from Depositor |
|---|---|
| Electronic file | Remarks, protocol and/or map (pdf) RDB06130.pdf from Depositor |
Original reference
| original | Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. |
|---|
Further references such as user reports and related articles (go to bottom)
Featured content
| Featured content | Expression Vector Backbone of Schizosaccharomyces pombe (English text) |
|---|
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB12155_A3H8.pdf |
|---|
Nucleotide sequence of a portion of this resource (if available).
| Primer: nmt1Fw | Sequence: RDB12155_A3H8a.seq |
|---|
|
>06130_12155_A3H8_nmt1Fw_A01_01.ab1 1 AGAAGTTTTA ATTTAAATCA GGAAAACGTA ACTCTCGGCT ACTGGATGGT TCAGTCACCC 61 AACGATTACT GGGGAGAGAA AACAGGGCAA AAGCAAAGCT TAAAGGAATC CGATTGTCAT 121 TCGGCAATGT GCAGCGAAAC TAAAAACCGG ATAATGGACC TGTTAATCGA AACATTGAAG 181 ATATATAAAG GAAGAGGAAT CCTGGCATAT CATCAATTGA ATAAGTTGAA TTAATTATTT 241 CAATCTCATT CTCACTTTCT GACTTATAGT CGCTTTGTTA AAGCTAGCGT CGACAGGCCT 301 GGATCCTATG CATCACCACC ATCATCATAT GGATTATAAG GATGATGACG ATAAACCCAT 361 GGACTACAAG GACGACGATG ACAAAGATAT CACAAGTTTG TACAAAAAAG CTGAACGAGA 421 AACGTAAAAT GATATAAATA TCAATATATT AAATTAGATT TTGCATAAAA AACAGACTAC 481 ATAATACTGT AAAACACAAC ATATCCAGTC ACTATGGCGG CCGCATTAGG CACCCCAGGC 541 TTTACACTTT ATGCTTCCGG CTCGTATAAT GTGTGGATTT TGAGTTAGGA TCCGTCGAGA 601 TTTTCAGGAG CTAAGGAAGC TAAAATGGAG AAAAAAATCA CTGGATATAC CACCGTTGAT 661 ATATCCCAAT GGCATCGTAA AGAACATTTT GAGGCATTTC AGTCAGTTGC TCAATGTACC 721 TATAACCAGA CCGTTCAGCT GGATATTACG GCCTTTTTAA AGACCGTAAA GAAAAATAAG 781 CACAAGTTTT ATCCGGCCTT TATTCACATT CTTGCCCGCC TGATGAATGC TCATCCGGAA 841 TTCCGTATGG CAATGAAAGA CGGTGAGCTG GTGATATGGG ATAGTGTTCA CCCTTGTTAC 901 ACCGTTTTCC ATGAGCAAAC TGAAACGTTT TCATCGCTCT GGAGTGAATA CCACGACGAT 961 TTCCGGCAGT TTCTACACAT ATATTCGCAA GATGTGGCGT GTTACGGTGA AAACCTGGCC 1021 CTATTTCCCT AAAGGGTTTA TTGAGATATG TTTTTCGTCT CAGCCAAATC CCTGGTGAGT 1081 TTCACAGTTT TGATTTAAAC CGTGATCGAT ATGGACCAAC TCCATCGCCC CCGATTTTCA 1141 CCATGGGGCC AAAATAATA // |
| Primer: ADH_rev | Sequence: RDB12155_A3H8b.seq |
|
>06130_12155_A3H8_ADHrev_B01_04.ab1 1 GGAAGAGTCT CAGGATAAGA TTTTCGTTTT AAACCTAAGA GTCACTTTAA AATTTGTATA 61 CACTTATTTT TTTTATAACT TATTTAATAA TAAAAATCAT AAATCATAAG AAATTCGCTT 121 ATTTAGAAGT GGCGCGCCGG ATCCTCTAGA GTCGAGATCT CATGATATCA CCACTTTGTA 181 CAAGAAAGCT GAACGAGAAA CGTAAAATGA TATAAATATC AATATATTAA ATTAGATTTT 241 GCATAAAAAA CAGACTACAT AATACTGTAA AACACAACAT ATCCAGTCAC TATGGTCGAC 301 CTGCAGACTG GCTGTGTATA AGGGAGCCTG ACATTTATAT TCCCCAGAAC ATCAGGTTAA 361 TGGCGTTTTT GATGTCATTT TCGCGGTGGC TGAGATCAGC CACTTCTTCC CCGATAACGG 421 AGACCGGCAC ACTGGCCATA TCGGTGGTCA TCATGCGCCA GCTTTCATCC CCGATATGCA 481 CCACCGGGTA AAGTTCACGG GAGACTTTAT CTGACAGCAG ACGTGCACTG GCCAGGGGGA 541 TCACCATCCG TCGCCCGGGC GTGTCAATAA TATCACTCTG TACATCCACA AACAGACGAT 601 AACGGCTCTC TCTTTTATAG GTGTAAACCT TAAACTGCAT TTCACCAGCC CCTGTTCTCG 661 TCAGCAAAAG AGCCGTTCAT TTCAATAAAC CGGGCGACCT CAGCCATCCC TTCCTGATTT 721 TCCGCTTTCC AGCGTTCGGC ACGCAGACGA CGGGCTTCAT TCTGCATGGT TGTGCTTACC 781 AGACCGGAGA TATTGACATC ATATATGCCT TGAGCAACTG ATAGCTGTCG CTGTCAACTG 841 TCACTGTAAT ACGCTGCTTC ATAGCATACC TCTTTTTTGA CATACTTCGG GTATACATAT 901 CAGTATATAT TCTTATACCG CAAAAATCAG CGCGCAAATA CGCATACTGT TATCTGGCTT 961 TTAGTAAGCC GGGATCCACG CGTTTACGCC CCGCCCTGCC ACTCATCGCA GTACTGTTGT 1021 AATTCATTAA GCATTCCTGC CGACATGGAA AGCCATCACA AACGGGCATG GATGATCTGG 1081 AATCGGCAGG CGGCATCAGC ACTGATCGCC TTTGCGTATA AATATTTTGC CCCAATGGGG 1141 TGAGA // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
| original | Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. |
|---|
2019.09.27
GNP_filter3_RDBDEP_html_190927.pl