Resource data sheet
Prev.

pDUAL-HFF1c

S. pombe 5 ter end His6-FLAG2-tagged vector with nmt1 promoter

Catalog number RDB06130
Resource name pDUAL-HFF1c
Clone info. S. pombe 5 ter end His6-FLAG2-tagged vector with nmt1 promoter. Multicopy marker, ura4+; Integration marker, leu1+.
Gateway compatible.
Vector backbone pDUAL (Plasmid)
Selectable markers E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Reference of nucleotide sequence AB364071.1 (DDBJ accession number)
Depositor Yoshida, Minoru | Matsuyama, Akihisa |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
Ordering Please visit Information of Request for Distribution. 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06130 pDUAL-HFF1c DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB06130z.seq from Depositor
Electronic file Remarks, protocol and/or map (pdf) RDB06130.pdf from Depositor

Original reference

original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12155_A3H8.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw | Sequence: RDB12155_A3H8a.seq
>06130_12155_A3H8_nmt1Fw_A01_01.ab1
    1 AGAAGTTTTA ATTTAAATCA GGAAAACGTA ACTCTCGGCT ACTGGATGGT TCAGTCACCC
   61 AACGATTACT GGGGAGAGAA AACAGGGCAA AAGCAAAGCT TAAAGGAATC CGATTGTCAT
  121 TCGGCAATGT GCAGCGAAAC TAAAAACCGG ATAATGGACC TGTTAATCGA AACATTGAAG
  181 ATATATAAAG GAAGAGGAAT CCTGGCATAT CATCAATTGA ATAAGTTGAA TTAATTATTT
  241 CAATCTCATT CTCACTTTCT GACTTATAGT CGCTTTGTTA AAGCTAGCGT CGACAGGCCT
  301 GGATCCTATG CATCACCACC ATCATCATAT GGATTATAAG GATGATGACG ATAAACCCAT
  361 GGACTACAAG GACGACGATG ACAAAGATAT CACAAGTTTG TACAAAAAAG CTGAACGAGA
  421 AACGTAAAAT GATATAAATA TCAATATATT AAATTAGATT TTGCATAAAA AACAGACTAC
  481 ATAATACTGT AAAACACAAC ATATCCAGTC ACTATGGCGG CCGCATTAGG CACCCCAGGC
  541 TTTACACTTT ATGCTTCCGG CTCGTATAAT GTGTGGATTT TGAGTTAGGA TCCGTCGAGA
  601 TTTTCAGGAG CTAAGGAAGC TAAAATGGAG AAAAAAATCA CTGGATATAC CACCGTTGAT
  661 ATATCCCAAT GGCATCGTAA AGAACATTTT GAGGCATTTC AGTCAGTTGC TCAATGTACC
  721 TATAACCAGA CCGTTCAGCT GGATATTACG GCCTTTTTAA AGACCGTAAA GAAAAATAAG
  781 CACAAGTTTT ATCCGGCCTT TATTCACATT CTTGCCCGCC TGATGAATGC TCATCCGGAA
  841 TTCCGTATGG CAATGAAAGA CGGTGAGCTG GTGATATGGG ATAGTGTTCA CCCTTGTTAC
  901 ACCGTTTTCC ATGAGCAAAC TGAAACGTTT TCATCGCTCT GGAGTGAATA CCACGACGAT
  961 TTCCGGCAGT TTCTACACAT ATATTCGCAA GATGTGGCGT GTTACGGTGA AAACCTGGCC
 1021 CTATTTCCCT AAAGGGTTTA TTGAGATATG TTTTTCGTCT CAGCCAAATC CCTGGTGAGT
 1081 TTCACAGTTT TGATTTAAAC CGTGATCGAT ATGGACCAAC TCCATCGCCC CCGATTTTCA
1141 CCATGGGGCC AAAATAATA
//
Primer: ADH_rev | Sequence: RDB12155_A3H8b.seq
>06130_12155_A3H8_ADHrev_B01_04.ab1
    1 GGAAGAGTCT CAGGATAAGA TTTTCGTTTT AAACCTAAGA GTCACTTTAA AATTTGTATA
   61 CACTTATTTT TTTTATAACT TATTTAATAA TAAAAATCAT AAATCATAAG AAATTCGCTT
  121 ATTTAGAAGT GGCGCGCCGG ATCCTCTAGA GTCGAGATCT CATGATATCA CCACTTTGTA
  181 CAAGAAAGCT GAACGAGAAA CGTAAAATGA TATAAATATC AATATATTAA ATTAGATTTT
  241 GCATAAAAAA CAGACTACAT AATACTGTAA AACACAACAT ATCCAGTCAC TATGGTCGAC
  301 CTGCAGACTG GCTGTGTATA AGGGAGCCTG ACATTTATAT TCCCCAGAAC ATCAGGTTAA
  361 TGGCGTTTTT GATGTCATTT TCGCGGTGGC TGAGATCAGC CACTTCTTCC CCGATAACGG
  421 AGACCGGCAC ACTGGCCATA TCGGTGGTCA TCATGCGCCA GCTTTCATCC CCGATATGCA
  481 CCACCGGGTA AAGTTCACGG GAGACTTTAT CTGACAGCAG ACGTGCACTG GCCAGGGGGA
  541 TCACCATCCG TCGCCCGGGC GTGTCAATAA TATCACTCTG TACATCCACA AACAGACGAT
  601 AACGGCTCTC TCTTTTATAG GTGTAAACCT TAAACTGCAT TTCACCAGCC CCTGTTCTCG
  661 TCAGCAAAAG AGCCGTTCAT TTCAATAAAC CGGGCGACCT CAGCCATCCC TTCCTGATTT
  721 TCCGCTTTCC AGCGTTCGGC ACGCAGACGA CGGGCTTCAT TCTGCATGGT TGTGCTTACC
  781 AGACCGGAGA TATTGACATC ATATATGCCT TGAGCAACTG ATAGCTGTCG CTGTCAACTG
  841 TCACTGTAAT ACGCTGCTTC ATAGCATACC TCTTTTTTGA CATACTTCGG GTATACATAT
  901 CAGTATATAT TCTTATACCG CAAAAATCAG CGCGCAAATA CGCATACTGT TATCTGGCTT
  961 TTAGTAAGCC GGGATCCACG CGTTTACGCC CCGCCCTGCC ACTCATCGCA GTACTGTTGT
 1021 AATTCATTAA GCATTCCTGC CGACATGGAA AGCCATCACA AACGGGCATG GATGATCTGG
 1081 AATCGGCAGG CGGCATCAGC ACTGATCGCC TTTGCGTATA AATATTTTGC CCCAATGGGG
1141 TGAGA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

2019.09.27

GNP_filter3_RDBDEP_html_190927.pl