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S. pombe 5 ter end His6-FLAG2-tagged vector with nmt1 promoter
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External Database Reference sequence |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation. |
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| Ordering | Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. Order form [Credit Card Payment ] [Bank Transfer Payment]Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word ] |
| Remarks | Use Survival2 (Invitrogen) or equivalent to amplify this clone. |
[open/close]| 提供条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、 寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。 |
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| 提供依頼 | 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word ]提供同意書 (MTA、非営利機関による非営利学術研究用)[Word ] |
| 備考 | このクローンの増殖にはSurvival2 (Invitrogen) あるいは同等の宿主を使用してください。 |
| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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| RDB06130 | pDUAL-HFF1c | DNA solution |
icon below as well as clone information before placing your order.Electronic file
| Sequence | RDB06130z.seq |
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| Remarks, protocol and/or map (pdf) | RDB06130.pdf |
Original reference
| original | Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. |
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Further references such as user reports and related articles (go to bottom)
Featured content
| Featured content | Expression Vector Backbone of Schizosaccharomyces pombe (English text) |
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RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
| Test sheet | RDB12155_A3H8.pdf |
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Nucleotide sequence of a portion of this resource (if available).
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Primer: nmt1Fw Sequence file: RDB12155_A3H8a.seq
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>06130_12155_A3H8_nmt1Fw_A01_01.ab1 1 AGAAGTTTTA ATTTAAATCA GGAAAACGTA ACTCTCGGCT ACTGGATGGT TCAGTCACCC 61 AACGATTACT GGGGAGAGAA AACAGGGCAA AAGCAAAGCT TAAAGGAATC CGATTGTCAT 121 TCGGCAATGT GCAGCGAAAC TAAAAACCGG ATAATGGACC TGTTAATCGA AACATTGAAG 181 ATATATAAAG GAAGAGGAAT CCTGGCATAT CATCAATTGA ATAAGTTGAA TTAATTATTT 241 CAATCTCATT CTCACTTTCT GACTTATAGT CGCTTTGTTA AAGCTAGCGT CGACAGGCCT 301 GGATCCTATG CATCACCACC ATCATCATAT GGATTATAAG GATGATGACG ATAAACCCAT 361 GGACTACAAG GACGACGATG ACAAAGATAT CACAAGTTTG TACAAAAAAG CTGAACGAGA 421 AACGTAAAAT GATATAAATA TCAATATATT AAATTAGATT TTGCATAAAA AACAGACTAC 481 ATAATACTGT AAAACACAAC ATATCCAGTC ACTATGGCGG CCGCATTAGG CACCCCAGGC 541 TTTACACTTT ATGCTTCCGG CTCGTATAAT GTGTGGATTT TGAGTTAGGA TCCGTCGAGA 601 TTTTCAGGAG CTAAGGAAGC TAAAATGGAG AAAAAAATCA CTGGATATAC CACCGTTGAT 661 ATATCCCAAT GGCATCGTAA AGAACATTTT GAGGCATTTC AGTCAGTTGC TCAATGTACC 721 TATAACCAGA CCGTTCAGCT GGATATTACG GCCTTTTTAA AGACCGTAAA GAAAAATAAG 781 CACAAGTTTT ATCCGGCCTT TATTCACATT CTTGCCCGCC TGATGAATGC TCATCCGGAA 841 TTCCGTATGG CAATGAAAGA CGGTGAGCTG GTGATATGGG ATAGTGTTCA CCCTTGTTAC 901 ACCGTTTTCC ATGAGCAAAC TGAAACGTTT TCATCGCTCT GGAGTGAATA CCACGACGAT 961 TTCCGGCAGT TTCTACACAT ATATTCGCAA GATGTGGCGT GTTACGGTGA AAACCTGGCC 1021 CTATTTCCCT AAAGGGTTTA TTGAGATATG TTTTTCGTCT CAGCCAAATC CCTGGTGAGT 1081 TTCACAGTTT TGATTTAAAC CGTGATCGAT ATGGACCAAC TCCATCGCCC CCGATTTTCA 1141 CCATGGGGCC AAAATAATA // |
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Primer: ADH_rev Sequence file: RDB12155_A3H8b.seq
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>06130_12155_A3H8_ADHrev_B01_04.ab1 1 GGAAGAGTCT CAGGATAAGA TTTTCGTTTT AAACCTAAGA GTCACTTTAA AATTTGTATA 61 CACTTATTTT TTTTATAACT TATTTAATAA TAAAAATCAT AAATCATAAG AAATTCGCTT 121 ATTTAGAAGT GGCGCGCCGG ATCCTCTAGA GTCGAGATCT CATGATATCA CCACTTTGTA 181 CAAGAAAGCT GAACGAGAAA CGTAAAATGA TATAAATATC AATATATTAA ATTAGATTTT 241 GCATAAAAAA CAGACTACAT AATACTGTAA AACACAACAT ATCCAGTCAC TATGGTCGAC 301 CTGCAGACTG GCTGTGTATA AGGGAGCCTG ACATTTATAT TCCCCAGAAC ATCAGGTTAA 361 TGGCGTTTTT GATGTCATTT TCGCGGTGGC TGAGATCAGC CACTTCTTCC CCGATAACGG 421 AGACCGGCAC ACTGGCCATA TCGGTGGTCA TCATGCGCCA GCTTTCATCC CCGATATGCA 481 CCACCGGGTA AAGTTCACGG GAGACTTTAT CTGACAGCAG ACGTGCACTG GCCAGGGGGA 541 TCACCATCCG TCGCCCGGGC GTGTCAATAA TATCACTCTG TACATCCACA AACAGACGAT 601 AACGGCTCTC TCTTTTATAG GTGTAAACCT TAAACTGCAT TTCACCAGCC CCTGTTCTCG 661 TCAGCAAAAG AGCCGTTCAT TTCAATAAAC CGGGCGACCT CAGCCATCCC TTCCTGATTT 721 TCCGCTTTCC AGCGTTCGGC ACGCAGACGA CGGGCTTCAT TCTGCATGGT TGTGCTTACC 781 AGACCGGAGA TATTGACATC ATATATGCCT TGAGCAACTG ATAGCTGTCG CTGTCAACTG 841 TCACTGTAAT ACGCTGCTTC ATAGCATACC TCTTTTTTGA CATACTTCGG GTATACATAT 901 CAGTATATAT TCTTATACCG CAAAAATCAG CGCGCAAATA CGCATACTGT TATCTGGCTT 961 TTAGTAAGCC GGGATCCACG CGTTTACGCC CCGCCCTGCC ACTCATCGCA GTACTGTTGT 1021 AATTCATTAA GCATTCCTGC CGACATGGAA AGCCATCACA AACGGGCATG GATGATCTGG 1081 AATCGGCAGG CGGCATCAGC ACTGATCGCC TTTGCGTATA AATATTTTGC CCCAATGGGG 1141 TGAGA // |
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Sequencing and PCR primers for primer information.
Original, user report and related articles
| original | Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. |
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2022.05.18
GNP_filter3_RDBDEP_html_220516.pl