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pcDNA3 FLAG hATF2 WT (#RDB06038)

Expression vector of FLAG-tagged human ATF2 WT

Alternative name U2500-10
Clone info. Expression vector of FLAG-tagged human ATF2 WT.
Comment Nucleotide substitution T1072G (Phe358Val) to NM_001880.
Vector backbone pcDNA3 (Plasmid)
Selectable markers Amp^r
Gene/insert name Human CREB2; ATF2 cDNA
Depositor|Developer Yokoyama, Kazunari | Inabe, Kumiko |
 

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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06038 pcDNA3 FLAG hATF2 WT DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3 FLAG hATF2 WT was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06038).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB06038_A6Cup1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB06038_A6Cua.seq check
>06038_06038_A6Cu_1_CMV-Forward_A04_01.ab1
    1 AGTCACTCGC CCATTGACGC AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
   61 AGAGCTCTCT GGCTAACTAG AGAACCCACT GCTTACTGGC TTATCGAAAT TAATACGACT
  121 CACTATAGGG AGACCCAAGC TTGGTACCGA GCTCGGATCC ACTAGTAACG GCCGCCAGTG
  181 TGCTGGAATT CTGCAGATCC ACCATGGACT ACAAGGACGA CGATGACAAG CTCGAGATGA
  241 AATTCAAGTT ACATGTGAAT TCTGCCAGGC AATACAAGGA CCTGTGGAAT ATGAGTGATG
  301 ACAAACCCTT TCTATGTACT GCGCCTGGAT GTGGCCAGCG TTTTACCAAC GAGGATCATT
  361 TGGCTGTCCA TAAACATAAA CATGAGATGA CACTGAAATT TGGTCCAGCA CGTAATGACA
  421 GTGTCATTGT GGCTGATCAG ACCCCAACAC CAACAAGATT CTTGAAAAAC TGTGAAGAAG
  481 TGGGTTTGTT TAATGAGTTG GCGAGTCCAT TTGAGAATGA ATTCAAGAAA GCTTCAGAAG
  541 ATGACATTAA AAAAATGCCT CTAGATTTAT CCCCTCTTGC AACACCTATC ATAAGAAGCA
  601 AAATTGAGGA GCCTTCTGTT GTAGAAACAA CTCACCAGGA TAGTCCTTTA CCTCACCCAG
  661 AGTCTACTAC CAGTGATGAG AAGGAAGTAC CATTGGCACA AACTGCACAG CCCACATCAG
  721 CTATTGTTCG TCCAGCATCA TTACAGGTTC CCAATGTGCT GCTTACAAGT TCTGACTCAA
  781 GTGTAATTAT TCAGCAGGCA GTACCTTCAC CAACCTCAAG TACTGTAATC ACCCAGGCAC
  841 CATCCTCTAA CAGGCCAATT GTCCCTGTAC CAGGCCCATT TCCTCTTCTG TTACATCTTC
  901 CTAATGGACA AACCATGCCT GTTGCTATTC CTGCATCAAT TACAAGTTCT AATGTGCATG
  961 TTCCAGCTGC AGTCCCACTC GTTCGACCAG TCACCATGGG TGCCCTAGTG TTTCCAGGAA
 1021 ATCCCAGGGT CCCTTTCCTC TCCCCACCAG TACAGTCAGA AAGCAAAAAT GAGATTTAAA
 1081 AAGCTGCTTT GACCAGCAAC ATCGTCCAGG TTACCGAAAT TGGTTGATAC TGTTCAAAGG
1141 TTCAATTGAT
//
Primer: BGH_rev2
Sequence file: RDB06038_A6Cub.seq check
>06038_06038_A6Cu_1_BGH_rev2_B04_04.ab1
    1 CATCGGTAAG TAGCACGGGG GAGGGGCAAC AACAGATGGC TGGCAACTAG AAGGCACAGT
   61 CGAGGCTGAT CAGCGAGCTC TAGCATTTAG GTGACACTAT AGAATAGGGC CCTCTAGATG
  121 CATGCTCGAG CGGCCGCCAG TGTGATGGAT CGGGATCCTC AACTTCCTGA GGGCTGTGAC
  181 TGGGAGGAAG GAGCCATAAC GATCTGTGAA AGAGCAGGCT CTGTACTCTG GTCCGCCATC
  241 TGGGTGAGGA CTGAAGTGGC TACAGCTTCT GCCTTGGAGG TTGAACTGAC TCCATTGGAT
  301 GTGCTGACCG AACTATGCTG TATAGCTTCT GTATGTGGAC TACTCGGCAC TGAAATGTCT
  361 TCTGAACTAT CATCTTTATC AGCAGTATGA TAGCCAGATT TCTTCTGCAT GGCGGTTACA
  421 GGGCAATCTT TATGAGCCAG AAGAAGCTGT TTCAGCTGTG CCACTTCATT TCTCAGCAGG
  481 GTGACTTCAC TCTGCAGCTG ACCATTTAAT GAACTCAAGT CTTCAGCTTT CTTCTCTAAA
  541 GACTGAACCC AGACTTTCCT TTTTTGTCGG CATCTTGAAG CTGCTGCTCT ATTTCGCTCT
  601 AAAACCTTTC TCCTTTTTTC ATCAGGATCT TCGTTAGCTG CTCTTCTCCG ACGACCACTT
  661 GTACTTTGGG TCTGTGGAGT TGTGTGAGCT GGAGAAGCCG GAGTTTCTGT AGTGGATGTG
  721 GCTGGCTGTT GTAATGACTG CGGTCGAGAT TCCTCTGACT GAGTCCTAAC CAATCCGCTA
  781 CCATGACCTT TGACAGTATC ACCATTGGTA ACTGGAGGAT GTTGCTGGGT CAAAGCAGCT
  841 TTTAATCTCA TTTTTGCTTC TGACTGTACT GGTTGGGGAG AGGAAGGACC TGGGATTCCT
  901 GGAACACTAG GCACCATGGT GACTGGTCGA ACGAGTGGGA CTGCAGCTGG AACATGCACA
  961 TTAGAACTTG TAATTGATGC AGGAATAGCA ACAGGCATGG TTTGTCCATT AGGAAGATGT
 1021 AACAGAAGAG GAAATGGGCC TGGTACAGGG ACAATTTGGG CCTGTTAGAA GAATGATGGC
 1081 TGCGTGGATA CAGTACTGAA GGTGGTTGAT GGTACTGCCT GCTGACTAAT ACACTGAGTC
1141 AGGAACTGCT AAGGCCAGGT TA
//
Primer:
Sequence file: RDB06038_A6Cuc.seq check
>06038_06038_A6Cu_1_SV40pro_F_C04_07.ab1
    1 GCTTGTCCCT TGGCATTCAG AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA
   61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC
  121 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
  181 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
  241 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
  301 TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
  361 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
  421 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
  481 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
  541 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
  601 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT
  661 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
  721 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA
  781 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA
  841 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG
  901 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGGTTC GAAATGACCG ACCAAGCGAC
  961 GCCCAACCTG CCATCACGAG ATTTCGATTC CAACCGCCGC CTTCTATGAA AGGTTTGGGC
 1021 TTCGGAATCG TTTTCCGGGA CGCCCGGCTG GATGATCCCT CCAGCGCGGG GATCTCATGC
 1081 TGGAGTTCTT CGTCCCACCC CAACTGTTAT TGCCAGCCTT ATAATGGGTT AACAAAATTA
1141 AAGGCCTA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

user_report Yamasaki T., Phosphorylation of Activation Transcription Factor-2 at Serine 121 by Protein Kinase C Controls c-Jun-mediated Activation of Transcription. J. Biol. Chem., 284, 8567-8581 (2009) PMID 19176525. [link to RRC of NBRP]