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pAdex GFAP-SwaI-SV40pA (#RDB06015)

Shuttle vector to generate rAd with GFAP promoter

Clone info. Shuttle vector to generate rAd. Inserted sequence is regulated under the control of GFAP promoter.
Comment GFAP-SwaI-SV40pA fragment (1.2k) was cloned on SwaI site of pAdex1cw. Produce adenovirus by COS-TPC method.
Vector backbone pAxcw (Cosmid, use packaging extracts for transforming E. coli host)
Selectable markers Amp^r
Gene/insert name Mouse GFAP
Depositor|Developer Hashimoto, Mitsuhiro |
 

Distribution information

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Remarks ((Additional form)) Approval Form(FormD)
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06015 pAdex GFAP-SwaI-SV40pA DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pAdex GFAP-SwaI-SV40pA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06015).

Reference section:

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13141_A5Br.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAdex_F
Sequence file: RDB13141_A5Bra.seq check
>06015_13141_A5Br_pAdex_F_E10_13.ab1
    1 CAGTTACGCA CACTCGCAGG GTCTGCACCT GGTGCGGGTC TCATCGTACC TCAGCACCTT
   61 CCAGATCCAT CGTCGCGAGT CGACAATCGA TTTGGTGGCG GCCGCTCTAG AACTAGTGGA
  121 TCCCCCGGGC TGCAGGAATT CGATATCAAG CTTGTAATTC GGTTTCCTCG GCCCTCTCTT
  181 GACCCATTTA CCAGAAAGGG GGTTCCTTGA TCAATGGCGA AGCCAGGCTG GTGTTCCCAA
  241 GAAAGCCTTG ACTCTGGGTA CAGTGACCTC AGTGGGGTGA GAGGAGTTCT CCCCTTAGCT
  301 GGGCTGGGGC CCAGCTCCAC CCCCTCAGGC TATTCAATGG GGGTGCTTCC AGGAAGTCAG
  361 GGGCAGATTT AGTCCAACCC GTTCCTCCAT AAAGGCCCTG ACATCCCAGG AGCCAGCAGA
  421 GGCAGGGCAA GCTTATCGGA TCCCCCATTT AAATCGGGCT GCAGGAATTC GATATCAAGC
  481 TTATCGGATC TTTGTGAAGG AACCTTACTT CTGTGGTGTG ACATAATTGG ACAAACTACC
  541 TACAGAGATT TAAAGCTCTA AGGTAAATAT AAAATTTTTA AGTGTATAAT GTGTTAAACT
  601 ACTGATTCTA ATTGTTTGTG TATTTTAGAT TCCAACCTAT GGAACTGATG AATGGGAGCA
  661 GTGGTGGAAT GCCTTTAATG AGGAAAACCT GTTTTGCTCA GAAGAAATGC CATCTAGTGA
  721 TGATGAGGCT ACTGCTGACT CTCAACATTC TACTCCTCCA AAAAAGAAGA GAAAGGTAGA
  781 AGACCCCAAG GACTTTCCTT CAGAATTGCT AAGTTTTTTG AGTCATGCTG TGTTTAGTAA
  841 TAGAACTCTT GCTTGCTTTG CTATTTACAC CACAAAGGAA AAAGCTGCAC TGCTATACAA
  901 GAAAATTATG GGAAAAATAT TCTGTAACCT TTATAAGTAG CATAACAGTT ATATCATAAC
  961 ATACTGTTTT TTCTACTCAC ACAGCATAGA GTGTCTGCTA TATACTATGC TCAAAATTGT
 1021 GTACTTTAGC TTTTACTTGT AAAGGGGTTA TAGGAATAAT TGATGGAATA TGCCCTTGGA
1081 CTGAGCAGTC TAATCAAGCC AGTACCACAC TTGATATAAG GGAGTATGAT
//
Primer: pJB8-Forward
Sequence file: RDB13141_A5Brb.seq check
>06015_13141_A5Br_pJB8-Forward_F10_16.ab1
    1 GATGCATATG ATATGAGGGG GTGGAGTTTG TGACGTGGCG CGGGGCGTGG GAACGGGGCG
   61 GGTGACGTAG TAGTGTGGCG GAAGTGTGAT GTTGCAAGTG TGGCGGAACA CATGTAAGCG
  121 ACGGATGTGG CAAAAGTGAC GTTTTTGGTG TGCGCCGGTG TACACAGGAA GTGACAATTT
  181 TCGCGCGGTT TTAGGCGGAT GTTGTAGTAA ATTTGGGCGT AACCGAGTAA GATTTGGCCA
  241 TTTTCGCGGG AAAACTGAAT AAGAGGAAGT GAAATCTGAA TAATTTTGTG TTACTCATAG
  301 CGCGTAATAT TTGTCTAGGG CCGCGGGGAC TTTGACCGTT TACGTGGAGA CTCGCCCAGG
  361 TGTTTTTCTC AGGTGTTTTC CGCGTTCCGG GTCAAAGTTG GCGTTTTATT ATTATAGTCA
  421 GCATCGATTT TCGAGGTCGA CGGTATCGGA TCCAGACATG ATAAGATACA TTGATGAGTT
  481 TGGACAAACC ACAACTAGAA TGCAGTGAAA AAAATGCTTT ATTTGTGAAA TTTGTGATGC
  541 TATTGCTTTA TTTGTAACCA TTATAAGCTG CAATAAACAA GTTAACAACA ACAATTGCAT
  601 TCATTTTATG TTTCAGGTTC AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA AGTAAAACCT
  661 CTACAAATGT GGTATGGCTG ATTATGATCT CTAGTCAAGG CACTATACAT CAAATATTCC
  721 TTATTAACCC CTTTACAAAT TAAAAAGCTA AAGGTACACA ATTTTTGAGC ATAGTTATTA
  781 ATAGCAGACA CTCTATGCCT GTGTGGAGTA AGAAAAAACA GTATGTTATG ATTATAACTG
  841 TTATGCCTAC TTATAAAGGT TACAGAATAT TTTTCCATAA TTTTCTTGTA TAGCAGTGCA
  901 GCTTTTTCCT TTGTGGTGTA AATAGCAAGC AAGCAGAGTT CTATTACTAA ACACAGCATG
  961 ACTCAAAAAC TAGCAATTCT GATGAAAGTC CTTGGGGTCT CCTACTTTCT CTTCTTTTTT
 1021 GGAGGAGGTA GATGGTGAGA GTCAGCAGTA GCCTCCATCA CTCACTCAGG ATGGGCAGTT
 1081 CTTCTGGAGC AGAACCGGAT TGTCCTCGTA GGCGAGTTCA TCACATGCTC TCTCCAGTCA
1141 ATTCAGATTG TCGCTTACGA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles