RDB05893 - pKM2L-phP21

Resource data sheet
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pKM2L-phP21

Promoter Bank clone, Human p21 cyclin dependent kinase inhibitor/WAF1/CIP1 promoter


Catalog number	RDB05893
Resource name	pKM2L-phP21
Alternative name	p1978-6F
Clone info.	The Human p21 cyclin dependent kinase inhibitor/WAF1/CIP1 promoter sequence (PCR-amplified 2,737 bp) was inserted into a Renilla luciferase reporter vector pKM2L (RDB4026). Forward primer, 1978 F2: 5' aaaagccagggctgcctctgctcaa 3'; Reverse primer, 1978 R2: 5' atccgcgcccagctccggctccaca 3' Entire sequence of promoter region has not been confirmed. (reference: J. Immunol., 172, 5006-5015, 2004, PMID:15067082)
Vector backbone	pKM2L (RDB4026) (Plasmid)
Size of vector backbone	4.2 kb
Selectable markers	Kan^r
Gene/insert name	Human p21 cyclin dependent kinase inhibitor/WAF1/CIP1 genomic DNA
Reference of nucleotide sequence	Z85996 (DDBJ accession number)
Depositor	DNA Bank, \|
Other clones in our bank	human CDKN1A (NCBI Gene 1026) \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.

Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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Terms and conditions set forth by the DEPOSITOR	1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional terms and conditions for distribution	The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
Ordering	Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word]

提供条件	1. 本件リソースは学術研究機関にのみ提供し、学術研究にのみ利用することができる。　2. 謝辞の表明を必要とする。
付加的提供条件	本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。
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Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB05893	pKM2L-phP21	DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file	Sequence RDB05893z.seq from Depositor

Featured content	Promoter Collection (Renilla Luciferase) (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet	RDB05893_A3Gop1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40	RDB05893_A3Goa.seq
>05893_05893_A3Go_M13(-40)_C10_07.ab1 1 CCGGCCGGTC CAGCTTGCAT GCCTGCAGGT CATTTAATAG GGATAACAGG GTAATGATTA 61 AGATCTCGAG CTCTAAGCTT CACATATGCA TGCACTAGTG GCAAAAGCCA GGGCTGCCTC 121 TGCTCAATAA TGTTCTATCT TTGTTCCGCC TCTTCTCTGG GGTCTCACTT CTTGGGAGCC 181 TGTGTGAAGG TGAATTCCTC TGAAAGCTGA CTGCCCCTAT TTGGGACTCC CCAGTCTCTT 241 TCTGAGAAAT GGTGACATTG TTCCCAGCAC TTCCTCTCCC TTCCTAGGCA GCTTCTGCAG 301 CCACCACTGA GCCTTCCTCA CATCCTCCTT CTTCAGGCTT GGGCTTTCCA CCTTTCACCA 361 TTCCCCTACC CCATGCTGCT CCACCGCACT CTGGGGAGGG GGCTGGACTG GGCACTCTTG 421 TCCCCCAGGC TGAGCCTCCC TCCATCCCTA TGCTGCCTGC TTCCCAGGAA CATGCTTGGG 481 CAGCAGGCTG TGGCTCTGAT TGGCTTTCTG GCCGTCAGGA ACATGTCCCA ACATGTTGAG 541 CTCTGGCATA GAAGAGGCTG GTGGCTATTT TGTCCTTGGG CTGCCTGTTT TCAGGTGAGG 601 AAGGGGATGG TAGGAGACAG GAGACCTCTA AAGACCCCAG GTAAACCTTA GCCTGTTACT 661 CTGAACAGGG TATGTGATCT GCCAGCAGAT CCTTGCGACA GGGCTGGGAT CTGATGCATG 721 TGTGCTTGTG TGAGTGTGTG CTGGGAGTCA GATTCTGTGT GTGACTTTTA ACAGCCTGCT 781 CCCTTGCCTT TTTCAGGGCA GAAGTCCTCC CTTAGAGTGT GTCTGGGTAC ACATTCAAGT 841 GCATGGTTGC AAACTTTTTT TTTTAAAGCA CTGAATAGTA CTAGACACTT AGTAGGTACT 901 TAAGAAATAT TGAATGTCGT GGTGGTGGTG AGCTAGAAGT TATAAAAAAA ATTCTTTCCC 961 AAAAACAACA ACAAAAAGAA TTATTTCATT GTGAAGCTCA GTACCACAAA AAATTTAAAT 1021 AATTCATTTA CAAGCCCTTT ATTAAAAAAA TTTTTCTCCC CAAGTAAAAC AGACAGACAT 1081 GTCTAGTCTA TTTTGAATTG CTTGAAGGCC AGACGGCCTT CAGCAGTGGG GAGAAGGGTG 1141 CCTGATTCT //
Primer: phRLR2	RDB05893_A3Gob.seq
>05893_05893_A3Go_phRLR2_D10_10.ab1 1 AACTTCCTGT CTGAAAGCCC AGTTTCTATT GGTCTCCTTA AACCTGTCTT GTAACCTTGA 61 TACTTACCTG CCCAGTGCCT CACGACCAAC TTCTGCAGTA CCCGGGTGAA TTCTACGCGT 121 CGACAGGCAT CCGCGCCCAG CTCCGGCTCC ACAAGGAACT GACTTCGGCA GCTGCTCACA 181 CCTCAGCTGG CGCAGCTCAG CGCGGCCCTG ATATACAACC GCCCCGCCCG GGCCCGCCTC 241 AAGGAGGCGG GACCCGCGCT CGGCCCACCG CGCCGCCCGG GACCGCCCTC CCCGGGGCCC 301 AGGCTCCTGG CTGCCCAGCG CCGAGCCAGC TGAGCCTGGC CGAGTTCCAG CAGGCCAGCC 361 GGTCCCGGAA CCTCGCGTGC TGCAGGAGGG CACTTCCCTC CTCCCCCAGT CCCTCGCCTG 421 CGTTGGTGCG CTGGACACAT TTCCCCACGA AGTGAGCCAC AAATCTGGCT TTTTTTACTT 481 GGAGAATGAG TTGGCACTCT CCAGGAGGAC ACAGCACTGT TAGAATGAGC CCCCTTTCTG 541 GCTCACCGCT GACCCACTCT GGCAGGCAAG GATTTACCCA ATGCAGCTGA AAAGATCAGG 601 AGGATGACAT TAATACATAA AAATTCATAA ATTATAAAAA CGATGCACCT CTCTGCAATT 661 TCCAGAAAAG CCCCACAATA TCACCTCTAT TCCCACTGAT CCCTCACTAG GTCACCTCTC 721 CCAGAAGCAC CTGGAGCACC TAGACACCCC AACAAAGCAT CTTGAGGCCA GAATGATTTT 781 TCAGTCCCGT TTATTTCACA GATGAGGAAA TTGAGGTCCA CTGAACTTAA GTATACAAAG 841 TTGTTGATTG TCACATGCTT CCGGGAAGGA GGGAATTGGA GAGACTACCA AAAAAGGGCA 901 ACCTGATCTC CAGGGAAACA GAAGAATTGG ACATTGAACC AATCTCCCTA CACCCTACAC 961 TCACCTGAAC AGAAGAACCC CTGTGGGTTG CAGCAGCTTT GTTGGCCAGG AAGGGAGGAT 1021 TTGACGAGTG AGTTGTCTGG TCTCTGATAC TCCCCACATA GCCGTATACA CTGCTTGGGA 1081 AACGGGGGGC TCAGAGTGTC TGATGACGTA TCTCAGATCA ATGCAGCCAA AGAAAATGAA 1141 CCTATATAGG //
Primer: pAxCALNL_F1	RDB05893_A3Goc.seq
>05893_05893_A3Go_pAxCALNL_F1_F12_06_ABI08.ab1 1 ACTAGGGAAT CTTTATCATG TCTGCTCGAA GCGGCCGCTC TAGAATTACT GCTCGTTCTT 61 CAGCACGCGC TCCACGAAGC TCTTGATGTA CTTACCCATT TCATCTGGAG CGTCCTCCTG 121 GCTGAAGTGG AGGCCCTTCA CCTTCACGAA CTCGGTGTTA GGGAACTTCT TAGCTCCCTC 181 GACAATAGCG TTGGAAAAGA ACCCAGGGTC GGACTCGATG AACATCTTAG GCAGATCGTC 241 GCTGGCCCGA AGGTAGGCGT TGTAGTTGCG GACAATCTGG ACGACGTCGG GCTTGCCTCC 301 CTTAACGAGA GGGATCTCGC GAGGCCAGGA GAGGGTAGGC CGTCTAACCT CGCCCTTCTC 361 CTTGAATGGC TCCAGGTAGG CAGCGAACTC CTCAGGCTCC AGTTTCCGCA TGATCTTGCT 421 TGGGAGCATG GTCTCGACGA AGAAGTTATT CTCAAGCACC ATTTTCTCGC CCTCTTCGCT 481 CTTGATCAGG GCGATATCCT CCTCGATGTC AGGCCACTCG TCCCAGGACT CGATCACGTC 541 CACGACACTC TCAGCATGGA CGATGGCCTT GATCTTGTCT TGGTGCTCGT AGGAGTAGTG 601 AAAGGCCAGA CAAGCCCCCC AGTCGTGGCC CACAAAGATG ATTTTCTTTG GAAGGTTCAG 661 CAGCTCGAAC CAAGCGGTGA GGTACTTGTA GTGATCCAGG AGGCGATATG AGCCATTCCC 721 GCTCTTGCCG GACTTACCCA TTCCGATCAG ATCAGGGATG ATGCATCTAG CCACGGGCTC 781 GATGTGAGGC ACGACGTGCC TCCACAGGTA GCTGGAGGCA GCGTTACCAT GCAGAAAAAT 841 CACGGCGTTT CTCGGCGTGC TTCTCGGGAA TCATAGTAGT TTGATGAAGG AGTCCAGCAC 901 GTTTCATTTG CTTGCAGCGA AGCCCCACCA CTGAGCCCAG TGATCATGCG TTTGCGTGCT 961 CGGGGTCGTA CACCTTGGAA GCAATGGTGT CTAGCCTATA GTTGAGTCGT ATAAGGTACT 1021 CTAGCTAGGA GCCTGTAATG GACCTGGTAT GGACCTGTGA AAGCAGCCAA GGTG //

Please visit Sequencing and PCR primers for primer information.

References

user report	Ishigro, N., ASPL-TFE3 Oncoprotein Regulates Cell Cycle Progression and Induces Cellular Senescence by Up-Regulating p21. Neoplasia 18 (10): 626-635 (2016). PMID 27673450.
user report	Suzuki, H., TATA-binding protein (TBP)-like protein is required for p53-dependent transcriptional activation of upstream promoter of p21Waf1/Cip1 gene. J. Biol. Chem., 287 (24): 19792-19803 (2012). PMID 22511763.
user report	Matsumura, K., Involvement of the estrogen receptor beta in genistein-induced expression of p21(waf1/cip1) in PC-3 prostate cancer cells. Anticancer Res., 28 (2A): 709-714 (2008). PMID 18507011.

2018.12.10

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