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pKM2L-phP21 (#RDB05893)

Promoter Bank clone, Human p21 cyclin dependent kinase inhibitor/WAF1/CIP1 promoter

Alternative name p1978-6F
Clone info. PCR amplified human p21 cyclin dependent kinase inhibitor/WAF1/CIP1 promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Cloned fragment: 36676033 to 36678769(NC_000006.12); relatively -2646 to +91, where +1 corresponds to 1 nt of NM_000389.4.
Comment PCR cloning, forward primer, 1978 F2: 5' aaaagccagggctgcctctgctcaa 3'; reverse primer, 1978 R2: 5' atccgcgcccagctccggctccaca 3' Entire sequence of promoter region has not been confirmed. (reference: J. Immunol., 172, 5006-5015, 2004, PMID:15067082)
Vector backbone pKM2L (RDB04026) (Plasmid)
Size of vector backbone 4.2 kb
Selectable markers Kan^r
Gene/insert name Human p21 cyclin dependent kinase inhibitor/WAF1/CIP1 genomic DNA
Depositor|Developer DNA Bank, |
 
Sequence RDB05893z.seq

Distribution information

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RDB05893 pKM2L-phP21 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pKM2L-phP21 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05893).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB05893_06Irp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 (Pr0410)
Region: insert5'
Sequence file: RDB05893_06Ira.seq check
>05893_05893_06Ir_M13_-40_C01_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNG NNNGCCTGCN GGTCNTTTAA ATAGGGNNAA CAGGGTAATG
   61 ATTAAGATCT CGAGCTCTAA GCTTCACATA TGCATGCACT AGTGGCAAAA GCCAGGGCTG
  121 CCTCTGCTCA ATAATGTTCT ATCTTTGTTC CGCCTCTTCT CTGGGGTCTC ACTTCTTGGG
  181 AGCCTGTGTG AAGGTGAATT CCTCTGAAAG CTGACTGCCC CTATTTGGGA CTCCCCAGTC
  241 TCTTTCTGAG AAATGGTGAC ATTGTTCCCA GCACTTCCTC TCCCTTCCTA GGCAGCTTCT
  301 GCAGCCACCA CTGAGCCTTC CTCACATCCT CCTTCTTCAG GCTTGGGCTT TCCACCTTTC
  361 ACCATTCCCC TACCCCATGC TGCTCCACCG CACTCTGGGG AGGGGGCTGG ACTGGGCACT
  421 CTTGTCCCCC AGGCTGAGCC TCCCTCCATC CCTATGCTGC CTGCTTCCCA GGAACATGCT
  481 TGGGCAGCAG GCTGTGGCTC TGATTGGCTT TCTGGCCGTC AGGAACATGT CCCAACATGT
  541 TGAGCTCTGG CATAGAAGAG GCTGGTGGCT ATTTTGTCCT TGGGCTGCCT GTTTTCAGGT
  601 GAGGAAGGGG ATGGTAGGAG ACAGGAGACC TCTAAAGACC CCAGGTAAAC CTTAGCCTGT
  661 TACTCTGAAC AGGGTATGTG ATCTGCCAGC AGATCCTTGC GACAGGGCTG GGATCTGATG
  721 CATGTGTGCT TGTGTGAGTG TGTGCTGGGA GTCAGATTCT GTGTGTGACT TTTAACAGCC
  781 TGCTCCCTTG CCTTTTTCAG GGCAGAAGTC CTCCCTTAGA GTGTGTCTGG NTACACATTC
  841 NAGTGCATGG TTGCNAACTT TTTTTTTTAA NGCACTGNAA TAGNACTANA CACTTAGTAN
  901 GNACTTAANA AATATTGAAT GTCGTGGTGG TGGTGAGCTA NAAGTTATAA AAAAAANTTC
  961 TTTTNCNNAA AACNANCNNC AAAAAGAANT NATTNNNTNN NTGAANNNNA NNANNNNAAA
1021 AAATTTAAAN NNANNNNNNN NNN
//
Primer: hRluc_N_rev (Pr0567)
Region: hRluc 5',insert 3'
Sequence file: RDB05893_06Irb.seq check
>05893_05893_06Ir__hRluc_N_rev_H01_08_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNTT TGCTTGNNGC GAGCCCNCNN CTGNNNGCCC AGTGATCNNN
   61 GCGTTTGCGT TGCTCGGGGT CGTACACCTT GGAAGCCATG GTGGCTAGCC TATAGTGAGT
  121 CGTATTAAGT ACTCTAGCCT TAAGAGCTGT AATTGAACTG GGAGTGGACA CCTGTGGAGA
  181 GAAAGGCAAA GTGGATGTCA GTAAGACCAA TAGGTGCCTA TCAGAAACGC AAGAGTCTTC
  241 TCTGTCTCGA CAAGCCCAGT TTCTATTGGT CTCCTTAAAC CTGTCTTGTA ACCTTGATAC
  301 TTACCTGCCC AGTGCCTCAC GACCAACTTC TGCAGTACCC GGGTGAATTC TACGCGTCGA
  361 CAGGCATCCG CGCCCAGCTC CGGCTCCACA AGGAACTGAC TTCGGCAGCT GCTCACACCT
  421 CAGCTGGCGC AGCTCAGCGC GGCCCTGATA TACAACCGCC CCGCCCGGGC CCGCCTCAAG
  481 GAGGCGGGAC CCGCGCTCGG CCCACCGCGC CGCCCGGGAC CGCCCTCCCC GGGGCCCAGG
  541 CTCCTGGCTG CCCAGCGCCG AGCCAGCTGA GCCTGGCCGA GTTCCAGCAG GCCAGCCGGT
  601 CCCGGAACCT CGCGTGCTGC AGGAGGGCAC TTCCCTCCTC CCCCAGTCCC TCGCCTGCGT
  661 TGGTGCGCTG GACACATTTC CCCACGAAGT GAGCCACAAA TCTGGCTTTT TTTACTTGGA
  721 GAATGAGTTG GCACTCTCCA GGAGGACACA GCACTGTTAG AATGAGCCCC CTTTCTGGCT
  781 CACCGCTGNN CCACTCTGGC AGGCAAGGAT TTACCCAATG CAGCTGAAAA AGATCAGGAN
  841 GANGACATTA ATACATAAAA ATTCATAAAT TATAAAAACG ATGCACCTCT CTGCANNTTC
  901 NANAAAAANC CCCNCNATAT CNCCTCTATT CCCACTGATN CCTCACTAGN TCACCTCTCC
  961 NANAANCNNC TGNANCANCT ANACNCCCCA ACAAANCNNC NTNGANNNCN NAANGATTTT
1021 TCAGNCCNNN TNNNTTCNCA NNNNNNNAAN NNGNNNNCCN NNNNNNNTNN NNNNNNNN
//
Primer: pAxCALNLF1 (Pr0166)
Region: hRluc mid
Sequence file: RDB05893_06Irc.seq check
>05893_05893_06Ir_pAxCALNLF1_D01_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNG TCTGNTCGNN NCGGCCGCTC TAGAATTACT GCTCGTTCTT
   61 CNGCACGCGC TCCACGAAGC TCTTGATGTA CTTACCCATT TCATCTGGAG CGTCCTCCTG
  121 GCTGAAGTGG AGGCCCTTCA CCTTCACGAA CTCGGTGTTA GGGAACTTCT TAGCTCCCTC
  181 GACAATAGCG TTGGAAAAGA ACCCAGGGTC GGACTCGATG AACATCTTAG GCAGATCGTC
  241 GCTGGCCCGA AGGTAGGCGT TGTAGTTGCG GACAATCTGG ACGACGTCGG GCTTGCCTCC
  301 CTTAACGAGA GGGATCTCGC GAGGCCAGGA GAGGGTAGGC CGTCTAACCT CGCCCTTCTC
  361 CTTGAATGGC TCCAGGTAGG CAGCGAACTC CTCAGGCTCC AGTTTCCGCA TGATCTTGCT
  421 TGGGAGCATG GTCTCGACGA AGAAGTTATT CTCAAGCACC ATTTTCTCGC CCTCTTCGCT
  481 CTTGATCAGG GCGATATCCT CCTCGATGTC AGGCCACTCG TCCCAGGACT CGATCACGTC
  541 CACGACACTC TCAGCATGGA CGATGGCCTT GATCTTGTCT TGGTGCTCGT AGGAGTAGTG
  601 AAAGGCCAGA CAAGCCCCCC AGTCGTGGCC CACAAAGATG ATTTTCTTTG GAAGGTTCAG
  661 CAGCTCGAAC CAAGCGGTGA GGTACTTGTA GTGATCCAGG AGGCGATATG AGCCATTCCC
  721 GCTCTTGCCG GACTTACCCA TTCCGATCAG ATCAGGGATG ATGCATCTAG CCACGGGCTC
  781 GATGTGAGGC ACGACGTGCC TCCNCAGGTA GCTGGAGGCA GCGTNACCAT GCAGAAAAAT
  841 CACGGCGTTC TCGGCGTGCT TNNCGNAATC ATAGNNNNTG ANGAANGANT CCAGCACGTT
  901 CATTTGCTTG CAGCGAGCCC NCCACTGANG NCCAGTGATC ATGCGTTTGN GTNGCTCGGG
  961 GNCNTANACN TNGNAAGNNN NGGTGNNNNN NNNATANNGA GTCGTATTNN NNNNNNTANN
1021 CNNTNNNNNN CTGNNATTGN NNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

user_report Ishigro, N., ASPL-TFE3 Oncoprotein Regulates Cell Cycle Progression and Induces Cellular Senescence by Up-Regulating p21. Neoplasia 18 (10): 626-635 (2016). PMID 27673450. [link to RRC of NBRP]
user_report Suzuki, H., TATA-binding protein (TBP)-like protein is required for p53-dependent transcriptional activation of upstream promoter of p21Waf1/Cip1 gene. J. Biol. Chem., 287 (24): 19792-19803 (2012). PMID 22511763. [link to RRC of NBRP]
user_report Matsumura, K., Involvement of the estrogen receptor beta in genistein-induced expression of p21(waf1/cip1) in PC-3 prostate cancer cells. Anticancer Res., 28 (2A): 709-714 (2008). PMID 18507011. [link to RRC of NBRP]