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pKM2L-phCLG4 (#RDB05479)

Promoter Bank clone, Human collagenase type IV (CLG4/MMP-2) promoter

Alternative name p1887-8F
Clone info. PCR amplified human collagenase type IV (CLG4/MMP-2) promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Cloned fragment: 55477535 to 55479249(NC_000016.10); relatively -1634 to +81, where +1 corresponds to 1 nt of NM_004530.5.
Comment PCR cloning, forward primer, G2PB0015F: 5' cacacccaccagacaagcctgaact 3'; reverse primer, G2Pb0015R: 5' aagccccagatgcgcagcctccag 3' Entire sequence of promoter region has not been confirmed.
Vector backbone pKM2L (RDB04026) (Plasmid)
Size of vector backbone 4.2 kb
Selectable markers Kan^r
Gene/insert name Human collagenase type IV (CLG4/MMP-2) genomic DNA
Depositor|Developer DNA Bank, |
 
Sequence RDB05479z.seq
Remarks, protocol and/or map (pdf) RDB05479.pdf

Distribution information

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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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RDB05479 pKM2L-phCLG4 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pKM2L-phCLG4 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05479).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB05479_A3Dfp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40
Sequence file: RDB05479_A3Dfa.seq check
>05479_05479_A3Df_M13(-40)_A08_02.ab1
    1 CGGCCGGGCA GGCTTGCATG CCTGCAGGTC ATTTAATAGG GATAACAGGG TAATGATTAA
   61 GATCTCGAGC TCTAAGCTTC ACATATGCAT GCACTAGTGG CCACACCCAC CAGACAAGCC
  121 TGAACTTGTC TGAAGCCCAC TGAGACCCAA GCCGCAGAGA CTTTTCTAGC TGTGATGATC
  181 AAGACATAAT CGTGACCTCC AATGCCCCCC ACAAGTATAT TGCTCCTGAT TCTTTCAGCC
  241 CCTGACCTTA CTTCTCAAAC TGTTCCCTGC TGACCCCCAG TCCTATCTGC CCGCTTCCTA
  301 GGCTGGTCCT TACTGACCCC TCCAGCTCCA TCCCCTCACC CTGTGCCCCA CCTTTTTCAG
  361 ATAGAAAAAA CTTTCTTCTC CAGTGCCTCT TGCTGTTTTT CATCTCTGGG CCATTGTCAA
  421 TGTTCCCTAA AACATTCCCC ATATTCCCCA CCCAGCACTC CACCTCTTTA GCTCTTCAGG
  481 TCTCAGCTCA GAAGTCACTT CTTCCAGGAA GCCTTCCTTG ATTGTCTTTA CTAGTTTAGG
  541 GGCTGAAGTC AGGCGTTCCC AACAGCCTGC TGGAGTTCCC CATCACAGCT TATCTCTCAA
  601 CTGTCTTTCC TGAGAGAGGG AGAAGACATT CCTCAGAGAC GGTTGTCACA GGGAGAACTT
  661 CAAAATTGGG ATTCGACCTG AGAGGCCACA TGGATTCTTG GCTTGGCGCA GGAAAGGATT
  721 CAAGAGTGAG TGGGGGAATT CGTGGAACTG AGGGCTCCTC CCCTTTTTAG ACCATATAGG
  781 GTAAACCTCC CCACATTGCC ATGGCATTTA TAAACTGCCA TGGCACTGGT GGGTGCTTCC
  841 TTTAACATGC TAATGCATTA TAATTAGCGT AAATGAGCAG TGAGGATGAC CAGAGGTCGC
  901 TTTCTTTGCC ATCTTGGTTT TGGGCTGGCT TCTTCACTGC ATACTGTTTT TATCAGTGGG
  961 GTCTTTTGTG ACTCTATCTT ATTAAACCCA GTCCTGCCAT TTCTATCTCA TTCCTGTGAC
 1021 GAGATGCGAC CCTCCTTGGG ATTGCAGCCA GCCAGGTCTC AGCCTCATTA GCTAGCCCTG
1081 ATCAGATGGA GTCGCTC
//
Primer: phRLR2
Sequence file: RDB05479_A3Dfb.seq check
>05479_05479_A3Df_phRLR2_B08_05.ab1
    1 ACTTTTCTGA CTCGACAGGC CCAGTTTCTA TTGGTCTCCT TAAACCTGTC TTGTAACCTT
   61 GATACTTACC TGCCCAGTGC CTCACGACCA ACTTCTGCAG TACCCGGGTG AATTCTACGC
  121 GTCGACAGGC AAGCCCCAGA TGCGCAGCCT CCAGCCACCG CCTGAGGAAG TCTGGATGCA
  181 GCGGAAACAA GGGAGGGCAG CCGCCAGATG TAGCCGGCTG GGCTGGGAGG GAGCTGGCAG
  241 AGCGGGGCTG GGGGCGGGGG GCGCACTCGG GCCCGCCCCT CTCTGCCCCA CCCCCCTACT
  301 CGCCCTCCTC CACTTTTCTC CTCTTTTTTC CTTTCTCCAC CTCTTTTCCC CGGCCGCCTG
  361 CTACTCCTGG CCTCTACGTC CACCCTCAGT GCACGACCTC GTCACCCCAC TTGCCTCTCT
  421 CGCGATCTGG GCGCACAGCC TCAGAACCCC CTGCTGGGAA ACATCTGTCG CTCTAGGCCC
  481 TTGACGTCCT GAGGTTAGAG AGCAGCGGTA CTACTAGGAG GGACGCCGGC TTGGCTAGGA
  541 CACCCTGCAC TCCCTGGGGT CAGGAACGGG AAGGAATGGT CAGAAACAGA TGATCAGCCA
  601 CCATGATAGA TGGTCAGAGA CAGAAATTTT CGATATTTAG GGATAGGGAC AGAGAGATTC
  661 TGGGATGAAC AGAGATGGAA AGAGAGAGAT GGAAACAGAG ACAGTGGAAG GTCCCAGGTT
  721 GCTTCCTTAC CTCTTTCCTT CTGCAAATGT GTAAGCCTTA ACTTGGCCTC GTATAGTGCG
  781 AGATGCTAGA TACACCTTGG GAAATAGAGC AGTCAGGGGC CCCGCGTTAG AGACGTTGGA
  841 ACCAGAGCGA CTCCATCTTG AACAGGGGGG CTGGGTAAAA TGAGGCTGAG ACCTGCTGGG
  901 CTGCACTCCC AGGAGGGTCC CGCATTCTCG GTCACAGGAT GAGATAGAAA TTGGGGCAAG
  961 ACTGGGTTTA ATAAAGATAG AGGTCACAAA GACCCCCACT GATAAAACAG TATGCAGTGA
 1021 AGAAGGCCAG GCCCAAAACC CAAGATGGCA AGAAAAGCCG ACTTCTGTCA TTCCTTCCAC
1081 TGCTCATTTT ACGCTTATTT ATAATGGCAT TTAGCAATGG TTAAAAGGAA AGAG
//
Primer: pAxCALNL_F1
Sequence file: RDB05479_A3Dfc.seq check
>05479_05479_A3Df_pAxCALNL_F1_C08_08.ab1
    1 TCATTATCTT ATCATGTCTG CTCGAGCGGC CGCTCTAGAA TTACTGCTCG TTCTTCAGCA
   61 CGCGCTCCAC GAAGCTCTTG ATGTACTTAC CCATTTCATC TGGAGCGTCC TCCTGGCTGA
  121 AGTGGAGGCC CTTCACCTTC ACGAACTCGG TGTTAGGGAA CTTCTTAGCT CCCTCGACAA
  181 TAGCGTTGGA AAAGAACCCA GGGTCGGACT CGATGAACAT CTTAGGCAGA TCGTCGCTGG
  241 CCCGAAGGTA GGCGTTGTAG TTGCGGACAA TCTGGACGAC GTCGGGCTTG CCTCCCTTAA
  301 CGAGAGGGAT CTCGCGAGGC CAGGAGAGGG TAGGCCGTCT AACCTCGCCC TTCTCCTTGA
  361 ATGGCTCCAG GTAGGCAGCG AACTCCTCAG GCTCCAGTTT CCGCATGATC TTGCTTGGGA
  421 GCATGGTCTC GACGAAGAAG TTATTCTCAA GCACCATTTT CTCGCCCTCT TCGCTCTTGA
  481 TCAGGGCGAT ATCCTCCTCG ATGTCAGGCC ACTCGTCCCA GGACTCGATC ACGTCCACGA
  541 CACTCTCAGC ATGGACGATG GCCTTGATCT TGTCTTGGTG CTCGTAGGAG TAGTGAAAGG
  601 CCAGACAAGC CCCCCAGTCG TGGCCCACAA AGATGATTTT CTTTGGAAGG TTCAGCAGCT
  661 CGAACCAAGC GGTGAGGTAC TTGTAGTGAT CCAGGAGGCG ATATGAGCCA TTCCCGCTCT
  721 TGCCGGACTT ACCCATTCCG ATCAGATCAG GGATGATGCA TCTAGCCACG GGCTCGATGT
  781 GAGGCACGAC GTGCCTCCAC AGGTAGCTGG GAGGCAGCGT TACCATGCAG AAAAATCACG
  841 GCGTTCTCGG CGTGCTTCTC GGAATCATAG TAGTTGATGA AAGGAGTCCA GCACGTTCAT
  901 TTGCTTGCAG CGAGCCCACC ACTGAGGCCC AGTGATCATG CGTTTGCGTT GCTCGGGGTC
  961 GTACACCTTT GGGAAAGCCA TGGGTGGGCT AGCCTATAGT GAGTCGTATT AAGTAACTCT
 1021 AGCGTAGAGC TGTAGTGACT GGGGAGTGGA CACTGTAAGA GAAAGGCAAG TTGTATGTCA
1081 GTTAGATCAA ATTAAGT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles