Resource data sheet
Prev.

Human HLA-C*14:02:01 cDNA

Human HLA-C*14:02:01 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB03378
Resource name Human HLA-C*14:02:01 cDNA
Alternative name HLA-Cw*14021
Clone info. Human HLA-C*14:02:01 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human HLA-Cw*14021 cDNA
Depositor Akatsuka, Yoshiki |
 
Other clones in our bank human HLA-C (NCBI Gene 3107) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)
Ordering Please visit Information of Request for Distribution. 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB03378 Human HLA-C*14:02:01 cDNA DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB03378z.seq from Depositor
Electronic file Map RDB03378.gif from Depositor

Featured content

Featured content HLA Clones (en) (English text)
Featured content HLA Clones (ja) (Japanese text)

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13697_A5Jrp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB13697_A5Jra.seq
>03378_13697_A5Jr_CMV-Forward_D11_11.ab1
    1 CTTAACTCGC CCATTGACGC AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
   61 AGAGCTCTCT GGCTAACTAG AGAACCCACT GCTTACTGGC TTATCGAAAT TAATACGACT
  121 CACTATAGGG AGACCCAAGC TGGCTAGCGT TTAAACTTAA GCTTTTCTCC CCAGACGCCG
  181 AGATGCGGGT CATGGCGCCC CGAACCCTCA TCCTGCTGCT CTCGGGAGCC CTGGCCCTGA
  241 CCGAGACCTG GGCCTGCTCC CACTCCATGA GGTATTTCTC CACATCCGTG TCCCGGCCCG
  301 GCCGCGGGGA GCCCCGCTTC ATCGCAGTGG GCTACGTGGA CGACACGCAG TTCGTGCGGT
  361 TCGACAGCGA CGCCGCGAGT CCGAGAGGGG AGCCGCGGGC GCCGTGGGTG GAGCAGGAGG
  421 GGCCGGAGTA TTGGGACCGG GAGACACAGA AGTACAAGCG CCAGGCACAG ACTGACCGAG
  481 TGAGCCTGCG GAACCTGCGC GGCTACTACA ACCAGAGCGA GGCCGGGTCT CACACCCTCC
  541 AGTGGATGTT TGGCTGCGAC CTGGGGCCCG ACGGGCGCCT CCTCCGCGGG TATGACCAGT
  601 CCGCCTACGA CGGCAAGGAT TACATCGCCC TGAACGAGGA TCTGCGCTCC TGGACCGCCG
  661 CGGACACGGC GGCTCAGATC ACCCAGCGCA AGTGGGAGGC GGCCCGTGAG GCGGAGCAGC
  721 GGAGAGCCTA CCTGGAGGGC ACGTGCGTGG AGTGGCTCCG CAGATACCTG GAGAACGGGA
  781 AGGAGACGCT GCAGCGCGCG GAACACCCAA AGACACACGT GACCCACCAT CCCGTCTCTG
  841 ACCATGAGGC CACCCTGAGG TGCTGGGCCC TGGGCTTCTA CCCTGCGGAG ATCACACTGA
  901 CCTGGCAGTG GGATGGGGGA GGACCAAACT CAGGACACCG AGCTTGTGGA GACCAGGCCA
  961 GCAGGAGATG GAAACCTTCC AGAAGTGGGC AGCTGTGGTG GGTGCCCTTC TGGAGAAGAG
 1021 CAGAGATACA CGTGCCCATG TGCAGCACGA GGGGCTGCCC GGAGCCCCTT CAACCTTGAG
1081 ATGGGTAGGT CGATCCTTCC CAGCCCACCC AATTCCCCCC AAT
//
Primer: BGH_rev RDB13697_A5Jrb.seq
>03378_13697_A5Jr_BGHrev_E11_14.ab1
    1 GGTCTATGGG GGCTCTAGAC TCGAGCGGCC GCGTCTCAGG CTTTACAAGC GATGAGAGAC
   61 TCATCAGAGC CCTGGGCACT GTTGCTGGAC GCAGCCTGAG AGCAGCTCCC TCCTTTTCCA
  121 CCTGAGCTCT TCCTCCTACA CATCACAACA GCCACCACAG CTCCTAGGAC AGCTAGGACA
  181 GCCAGGACAG CCAGGCCAGC AACGATGCCC ACGATGGGGA TGGTGGGCTG GGAAGACGGC
  241 TCCCATCTCA GGGTGAGGGG CTCCGGCAGC CCCTCGTGCT GCACATGGCA CGTGTATCTC
  301 TGCTCTTCTC CAGAAGGCAC CACCACAGCT GCCCACTTCT GGAAGGTTCC ATCTCCTGCT
  361 GGCCTGGTCT CCACAAGCTC GGTGTCCTGA GTTTGGTCCT CCCCATCCCA CTGCCAGGTC
  421 AGTGTGATCT CCGCAGGGTA GAAGCCCAGG GCCCAGCACC TCAGGGTGGC CTCATGGTCA
  481 GAGACGGGAT GGTGGGTCAC GTGTGTCTTT GGGTGTTCCG CGCGCTGCAG CGTCTCCTTC
  541 CCGTTCTCCA GGTATCTGCG GAGCCACTCC ACGCACGTGC CCTCCAGGTA GGCTCTCCGC
  601 TGCTCCGCCT CACGGGCCGC CTCCCACTTG CGCTGGGTGA TCTGAGCCGC CGTGTCCGCG
  661 GCGGTCCAGG AGCGCAGATC CTCGTTCAGG GCGATGTAAT CCTTGCCGTC GTAGGCGGAC
  721 TGGTCATACC CGCGGAGGAG GCGCCCGTCG GGCCCCAGGT CGCAGCCAAA CATCCACTGG
  781 AGGGTGTGAG ACCCGGCCTC GCTCTGGTTG TAGTAGCCGC GCAGGTTCCG CAGGCTCACT
  841 CGGTCAGTCT GTGCCTGGCG CTTGTACTTC TGTGTCTCCC GGTCCCAATA CTCCGGCCCC
  901 TCCTGCTCCA CCCACGGCGC CCGCGGCTCC CCTCTCGGAC TCGCGGCGTC GCTGTCGAAC
  961 CGCACGAACT GCGTGTCGTC CACGTAGCCC ACTGCGATGA AGCGGGGGCT CCCCCGCGGG
 1021 CCCGGGCCCG GGACACGGGA TGTGGGAGAA AATACCCTCA TGGCAGTGGG AGCAGGCCCA
1081 AGTCTCGGTC AAGGACCAGG GCTCCCCGGA AGAAGCCAGC CAGGGAATGT GGAAG
//
Primer: SV40pro_F_V2 RDB13697_A5Jrc.seq
>03378_13697_A5Jr_SV40pro_F_V2_F11_17.ab1
    1 GGGGCTTTTT TGGAGCTAGG CTTTTGCAAA AGCTCCCGGG AGCTTGTATA TCCATTTTCG
   61 GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG
  121 CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA
  181 TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG
  241 TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT
  301 GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA
  361 GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC
  421 CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG
  481 CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG
  541 AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG
  601 AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG
  661 GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT
  721 GTGGCCGGCT GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG
  781 CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC
  841 CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA GCGGGACTCT
  901 GGGGTTCGAA ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT TCGATTCCAC
  961 CGCCGCCTTC TATGAAGGTT GGGCTTCGGA ATCGTTTTTC CGGGACGCCC GGCTGGAATG
 1021 ATCCTCCAGC GGCGGGGGAT CTCATGCCTG GAGGTTCTTC GGCCCAACCC CAACTGTTAA
1081 TGGCCAGCCT TTATATGGCT TAACGAACTA AAGTCAATAA GC
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

2018.12.11

GNP_filter3_RDBDEP_html_181012.pl