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Human HLA-C*14:02:01 cDNA

Human HLA-C*14:02:01 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB03378
Resource name Human HLA-C*14:02:01 cDNA
Alternative name HLA-Cw*14021
Clone info. Human HLA-C*14:02:01 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human HLA-Cw*14021 cDNA
Depositor|Developer Akatsuka, Yoshiki |
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External Database
Human HLA-Cw*14021

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
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          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB03378 Human HLA-C*14:02:01 cDNA DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence RDB03378z.seq
          Remarks, protocol and/or map (gif) RDB03378.gif

          Original reference

          original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content HLA Clones (en) (English text)
          Featured content HLA Clones (ja) (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13697_A5Jrp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward
          Region: CMV pro,insert 5'
          Sequence file: RDB13697_A5Jra.seq check
          >03378_13697_A5Jr_CMV-Forward_D11_11.ab1
              1 NNNNNNNNNN NNNTTNNNGC NNTGGGCGGT ANGCGTGTAC GGTGGGAGGT CTATATAAGC
             61 AGAGCTCTCT GGCTAACTAG AGAACCCACT GCTTACTGGC TTATCGAAAT TAATACGACT
            121 CACTATAGGG AGACCCAAGC TGGCTAGCGT TTAAACTTAA GCTTTTCTCC CCAGACGCCG
            181 AGATGCGGGT CATGGCGCCC CGAACCCTCA TCCTGCTGCT CTCGGGAGCC CTGGCCCTGA
            241 CCGAGACCTG GGCCTGCTCC CACTCCATGA GGTATTTCTC CACATCCGTG TCCCGGCCCG
            301 GCCGCGGGGA GCCCCGCTTC ATCGCAGTGG GCTACGTGGA CGACACGCAG TTCGTGCGGT
            361 TCGACAGCGA CGCCGCGAGT CCGAGAGGGG AGCCGCGGGC GCCGTGGGTG GAGCAGGAGG
            421 GGCCGGAGTA TTGGGACCGG GAGACACAGA AGTACAAGCG CCAGGCACAG ACTGACCGAG
            481 TGAGCCTGCG GAACCTGCGC GGCTACTACA ACCAGAGCGA GGCCGGGTCT CACACCCTCC
            541 AGTGGATGTT TGGCTGCGAC CTGGGGCCCG ACGGGCGCCT CCTCCGCGGG TATGACCAGT
            601 CCGCCTACGA CGGCAAGGAT TACATCGCCC TGAACGAGGA TCTGCGCTCC TGGACCGCCG
            661 CGGACACGGC GGCTCAGATC ACCCAGCGCA AGTGGGAGGC GGCCCGTGNN GCGGANCAGC
            721 GGANAGCCTA CCTGGAGGGC ACGTGCGTGG AGTGGCTCCG CAGATACCTG GAGAACGGGA
            781 AGGAGACGCT GCAGCGCGCG GAACACCCAA AGACACACGT GANCCACCAT CCCGTCTCTG
            841 ACCATGNNGN CACCCNNNNG TGCTGNGCCC NNGGCTTCTA CCCTGCGGNN NTCACACNGN
            901 NNNNGCANNG GNNTGGGGGA NGNNCNAACT CANGANACCN NNCTNNNNNA NNNCNNNNNA
          961 GCNNNANNNN NNANNNNNNC NNNNNNNGGN NNNNNNNNN
          //
          Primer: BGH_rev2
          Region: bGHpA,insert 3'
          Sequence file: RDB13697_A5Jrb.seq check
          >03378_13697_B0FtS_BGH_rev2_H03_08_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NGGNNGGGCN NACAACAGAT GGCTGGCAAC TAGAAGGCAC
             61 AGTCGAGGCT GATCAGCGGG TTTAAACGGG CCCTCTAGAC TCGAGCGGCC GCGTCTCAGG
            121 CTTTACAAGC GATGAGAGAC TCATCAGAGC CCTGGGCACT GTTGCTGGAC GCAGCCTGAG
            181 AGCAGCTCCC TCCTTTTCCA CCTGAGCTCT TCCTCCTACA CATCACAACA GCCACCACAG
            241 CTCCTAGGAC AGCTAGGACA GCCAGGACAG CCAGGCCAGC AACGATGCCC ACGATGGGGA
            301 TGGTGGGCTG GGAAGACGGC TCCCATCTCA GGGTGAGGGG CTCCGGCAGC CCCTCGTGCT
            361 GCACATGGCA CGTGTATCTC TGCTCTTCTC CAGAAGGCAC CACCACAGCT GCCCACTTCT
            421 GGAAGGTTCC ATCTCCTGCT GGCCTGGTCT CCACAAGCTC GGTGTCCTGA GTTTGGTCCT
            481 CCCCATCCCA CTGCCAGGTC AGTGTGATCT CCGCAGGGTA GAAGCCCAGG GCCCAGCACC
            541 TCAGGGTGGC CTCATGGTCA GAGACGGGAT GGTGGGTCAC GTGTGTCTTT GGGTGTTCCG
            601 CGCGCTGCAG CGTCTCCTTC CCGTTCTCCA GGTATCTGCG GAGCCACTCC ACGCACGTGC
            661 CCTCCAGGTA GGCTCTCCGC TGCTCCGCCT CACGGGCCGC CTCCCACTTG CGCTGGGTGA
            721 TCTGAGCCGC CGTGTCCGCG GCGGTCCAGG ANCGCAGATN CTCGNTCNNG NCGANGTAAT
            781 CCTTGCCGNC GTNNNGGANT NNNCATACCC GNGGNNGANG CGCCCGTCGG GCCCCAGGNC
            841 NNANNNAAAC NTCCNNNNGN NGGNGNNANA NCNNGNCNCG NTNNGNNNNN ANNANCCNNN
          901 CANNNNNNNN NN
          //
          Primer: SV40pro_F
          Region: NeoR
          Sequence file: RDB13697_A5Jrc.seq check
          >03378_13697_A5Jr_SV40pro_F_V2_F11_17.ab1
              1 NNNNNNNNNN NNNNNCNNNG CTTTTGCAAA AGCTCCCGGG NNCTTGTATA TCCNTTTTCG
             61 GATCTGATCA ANNNNNAGGN TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG
            121 CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA
            181 TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG
            241 TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT
            301 GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA
            361 GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC
            421 CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG
            481 CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG
            541 AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG
            601 AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGNTCTCGTC GTGACCCATG
            661 GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT
            721 GTGGCCGGCT GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG
            781 CTGAAGANCT TGGCGGCGAA TGGGCTGANC GCTTCCTCNT GCTTTACGGT ATCGCCGCTC
            841 CNGATTCGCA GCGCATCGCC TTCTATCGCC TTCTNGACNA NTTCTNCTGA NCGGNNNTCN
            901 NGGGTTCNAA ATGNNCGANN NAGCGNNNNN NNACNNNNNN TCNCNANANT TCNNNTCCNN
          961 NNNNNNCNTC NNNNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

          2022.02.09

          GNP_filter3_RDBDEP_html_220112.pl