Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

Human HLA-C*14:02:01 cDNA

Human HLA-C*14:02:01 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB03378
Resource name Human HLA-C*14:02:01 cDNA
Alternative name HLA-Cw*14021
Clone info. Human HLA-C*14:02:01 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human HLA-Cw*14021 cDNA
Depositor|Developer Akatsuka, Yoshiki |
 
Other clones in our bank human HLA-C (NCBI Gene 3107) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)
Ordering Please visit Information of Request for Distribution.[link] 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB03378 Human HLA-C*14:02:01 cDNA DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Sequence RDB03378z.seq
Electronic file Map RDB03378.gif

Original reference

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content HLA Clones (en) (English text)
Featured content HLA Clones (ja) (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13697_A5Jrp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward
Region: CMV pro,insert 5'
Sequence file: RDB13697_A5Jra.seq check
>03378_13697_A5Jr_CMV-Forward_D11_11.ab1
    1 NNNNNNNNNN NNNTTNNNGC NNTGGGCGGT ANGCGTGTAC GGTGGGAGGT CTATATAAGC
   61 AGAGCTCTCT GGCTAACTAG AGAACCCACT GCTTACTGGC TTATCGAAAT TAATACGACT
  121 CACTATAGGG AGACCCAAGC TGGCTAGCGT TTAAACTTAA GCTTTTCTCC CCAGACGCCG
  181 AGATGCGGGT CATGGCGCCC CGAACCCTCA TCCTGCTGCT CTCGGGAGCC CTGGCCCTGA
  241 CCGAGACCTG GGCCTGCTCC CACTCCATGA GGTATTTCTC CACATCCGTG TCCCGGCCCG
  301 GCCGCGGGGA GCCCCGCTTC ATCGCAGTGG GCTACGTGGA CGACACGCAG TTCGTGCGGT
  361 TCGACAGCGA CGCCGCGAGT CCGAGAGGGG AGCCGCGGGC GCCGTGGGTG GAGCAGGAGG
  421 GGCCGGAGTA TTGGGACCGG GAGACACAGA AGTACAAGCG CCAGGCACAG ACTGACCGAG
  481 TGAGCCTGCG GAACCTGCGC GGCTACTACA ACCAGAGCGA GGCCGGGTCT CACACCCTCC
  541 AGTGGATGTT TGGCTGCGAC CTGGGGCCCG ACGGGCGCCT CCTCCGCGGG TATGACCAGT
  601 CCGCCTACGA CGGCAAGGAT TACATCGCCC TGAACGAGGA TCTGCGCTCC TGGACCGCCG
  661 CGGACACGGC GGCTCAGATC ACCCAGCGCA AGTGGGAGGC GGCCCGTGNN GCGGANCAGC
  721 GGANAGCCTA CCTGGAGGGC ACGTGCGTGG AGTGGCTCCG CAGATACCTG GAGAACGGGA
  781 AGGAGACGCT GCAGCGCGCG GAACACCCAA AGACACACGT GANCCACCAT CCCGTCTCTG
  841 ACCATGNNGN CACCCNNNNG TGCTGNGCCC NNGGCTTCTA CCCTGCGGNN NTCACACNGN
  901 NNNNGCANNG GNNTGGGGGA NGNNCNAACT CANGANACCN NNCTNNNNNA NNNCNNNNNA
961 GCNNNANNNN NNANNNNNNC NNNNNNNGGN NNNNNNNNN
//
Primer: BGH_rev2
Region: bGHpA,insert 3'
Sequence file: RDB13697_A5Jrb.seq check
>03378_13697_B0FtS_BGH_rev2_H03_08_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NGGNNGGGCN NACAACAGAT GGCTGGCAAC TAGAAGGCAC
   61 AGTCGAGGCT GATCAGCGGG TTTAAACGGG CCCTCTAGAC TCGAGCGGCC GCGTCTCAGG
  121 CTTTACAAGC GATGAGAGAC TCATCAGAGC CCTGGGCACT GTTGCTGGAC GCAGCCTGAG
  181 AGCAGCTCCC TCCTTTTCCA CCTGAGCTCT TCCTCCTACA CATCACAACA GCCACCACAG
  241 CTCCTAGGAC AGCTAGGACA GCCAGGACAG CCAGGCCAGC AACGATGCCC ACGATGGGGA
  301 TGGTGGGCTG GGAAGACGGC TCCCATCTCA GGGTGAGGGG CTCCGGCAGC CCCTCGTGCT
  361 GCACATGGCA CGTGTATCTC TGCTCTTCTC CAGAAGGCAC CACCACAGCT GCCCACTTCT
  421 GGAAGGTTCC ATCTCCTGCT GGCCTGGTCT CCACAAGCTC GGTGTCCTGA GTTTGGTCCT
  481 CCCCATCCCA CTGCCAGGTC AGTGTGATCT CCGCAGGGTA GAAGCCCAGG GCCCAGCACC
  541 TCAGGGTGGC CTCATGGTCA GAGACGGGAT GGTGGGTCAC GTGTGTCTTT GGGTGTTCCG
  601 CGCGCTGCAG CGTCTCCTTC CCGTTCTCCA GGTATCTGCG GAGCCACTCC ACGCACGTGC
  661 CCTCCAGGTA GGCTCTCCGC TGCTCCGCCT CACGGGCCGC CTCCCACTTG CGCTGGGTGA
  721 TCTGAGCCGC CGTGTCCGCG GCGGTCCAGG ANCGCAGATN CTCGNTCNNG NCGANGTAAT
  781 CCTTGCCGNC GTNNNGGANT NNNCATACCC GNGGNNGANG CGCCCGTCGG GCCCCAGGNC
  841 NNANNNAAAC NTCCNNNNGN NGGNGNNANA NCNNGNCNCG NTNNGNNNNN ANNANCCNNN
901 CANNNNNNNN NN
//
Primer: SV40pro_F
Region: NeoR
Sequence file: RDB13697_A5Jrc.seq check
>03378_13697_A5Jr_SV40pro_F_V2_F11_17.ab1
    1 NNNNNNNNNN NNNNNCNNNG CTTTTGCAAA AGCTCCCGGG NNCTTGTATA TCCNTTTTCG
   61 GATCTGATCA ANNNNNAGGN TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG
  121 CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA
  181 TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG
  241 TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT
  301 GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA
  361 GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC
  421 CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG
  481 CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG
  541 AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG
  601 AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGNTCTCGTC GTGACCCATG
  661 GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT
  721 GTGGCCGGCT GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG
  781 CTGAAGANCT TGGCGGCGAA TGGGCTGANC GCTTCCTCNT GCTTTACGGT ATCGCCGCTC
  841 CNGATTCGCA GCGCATCGCC TTCTATCGCC TTCTNGACNA NTTCTNCTGA NCGGNNNTCN
  901 NGGGTTCNAA ATGNNCGANN NAGCGNNNNN NNACNNNNNN TCNCNANANT TCNNNTCCNN
961 NNNNNNCNTC NNNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

2020.10.02

GNP_filter3_RDBDEP_html_200316.pl