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pCMX-N/VP16-RBP-J

Expression vector of mouse RBP-J

Catalog number RDB03023
Resource name pCMX-N/VP16-RBP-J
Alternative name pCMX-N/VP16-RBP-2
Clone info. Expression vector of constitutive active mouse RBP-J (VP16-RBP-J). Activation domain of VP16 was followed by N-terminal of RBP-J. Wild type (pCMX-N/RBP-J, RDB03021) is available.
Vector backbone pCMX-N/VP16 (Plasmid)
Size of vector backbone 4,785 bp
Selectable markers Amp^r
Gene/insert name Mouse RBP-J cDNA
Depositor|Developer Honjo, Tasuku | Kuroda, Kazuki |
Other clones in our bank

External Database
Mouse RBP-J

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Kyoto University Medical Science and Business Liaison Organization Liaison Office (contracts@kumbl.med.kyoto-u.ac.jp). Acknowledgement to Depositor in publication. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the name of resource, RECIPIENT's name and his/her affiliation, purpose of the use, and number of resources.
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          提供条件 営利機関の利用者は、事前に京都大学・「医学領域」産学連携推進機構・知財室 (contracts@kumbl.med.kyoto-u.ac.jp) に連絡し、承諾を得ること。研究結果の発表時、謝辞に寄託者名を入れ ること。理研BRCは、本件リソースのリソース名、提供先、利用目的、件数を京都大学・本庶佑教授に報告する。
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          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB03023 pCMX-N/VP16-RBP-J DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (gif) RDB03023.gif

          Original reference

          original Kuroda, K., Delta-induced Notch signaling mediated by RBP-J inhibits MyoD expression and myogenesis. J. Biol. Chem., 274, 7238-7244 (1999). PMID 10066785.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB12303_A3Jm.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer:
          Sequence file: RDB12303_A3Jma.seq check
          >03023_12303_A3Jm_CMV-Forward_H06_24.ab1
              1 CGTCACTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
             61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC
            121 TCACTATAGG GAGACCCAAG CTTGCCACCA TGGCCCCCCC GACCGATGTC AGCCTGGGGG
            181 ACGAGCTCCA CTTAGACGGC GAGGACGTGG CGATGGCGCA TGCCGACGCG CTAGACGATT
            241 TCGATCTGGA CATGTTGGGG GACGGGGATT CCCCGGGTCC GGGATTTACC CCCCACGACT
            301 CCGCCCCCTA CGGCGCTCTG GATATGGCCG ACTTCGAGTT TGAGCAGATG TTTACCGATG
            361 CCCTTGGAAT TGACGAGTAC GGTGGGAAGC TTCTAGAATT CATGCCCTCC GGTTTTCCTC
            421 AGTCTCCACG TACGTCCCCG AGGGCGCGTC CCAAAACCCG GATAACCGGA GCGCTCCCCA
            481 TGGACTACTC GGAGGGCTTG TCCGCGGAGG AGCGGCCTGC GCATGCTCCA TCGGCGGGGA
            541 AGTTTGGTGA GCGGCCTCCA CCCAAACGAC TCACTAGGGA AGCTATGCGA AATTATTTAA
            601 AAGAACGAGG GGATCAAACA GTGCTCATTC TTCATGCAAA AGTTGCACAG AAGTCTTACG
            661 GAAATGAAAA ACGATTTTTT TGCCCTCCTC CTTGTGTGTA TCTTATGGGC AGTGGTTGGA
            721 AGAAAAAAAA AGAGCAAATG GAACGAGATG GATGTTCTGA ACAAGAGTCT CAACCCTGTG
            781 CGTTTATTGG AATAGGAAAC AGTGACCAAG AAATGCAGCA GCTGAACTTG GAAGGGAAGA
            841 ACTACTGCAC AGCCAAAACA TTGTACATAT CTGATTCAGA CAAGAGAAAA CATTTCATGT
            901 TGTCTGTAAA GATGTTCTAT GGCAACAGCG ATGACATTGG TGTGTTCCTC AGCAAGCGGA
            961 TAAAGGTCAT CTCCAAACCC TCCAAAAAGA AGCAGTCACT GAAGAATGCT GACTTGTGCA
           1021 TTGCTTCAGG AACGAAGTGG CACTGTTCAT CGCTTTCGGT CCCAGACAGT TAGTACCAGG
           1081 TACTGCATGT AGAAGGAGGG AATTCACGCA AGTTCACACC AGCTGGGGAG CCATTTTAAC
          1141 ATCCAATCTC TCTTTGGACC GAACGAG
          //
          Primer:
          Sequence file: RDB12303_A3Jmb.seq check
          >03023_12303_A3Jm_pAxEFR2_E01_13.ab1
              1 CGTGTCAAAT GCTTTATTTG TGAATTTGTG ATGCTATTGC TTTATTTGTA ACCATTATAA
             61 GCTGCAATAA ACAAGTTAAC AACAACAATT GCATTCATTT TATGTTTCAG GTTCAGGGGG
            121 AGGTGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGGTATG GCTGATTATG
            181 ATCTCTAGTC AAGGCACTAT ACATCAAATA TTTTTCCATA ATTTTCTTGT ATAGCAGTGC
            241 AGCTTTTTCC TTTGTGGTGT AAATAGCAAA GCAAGCAAGA GTTCTATTAC TAAACACAGC
            301 ATGACTCAAA AAACTTAGCA ATTCTGAAGG AAAGTCCTTG GGGTCTTCTA CCTTTCTCTT
            361 CTTTTTTGGA GGAGTAGAAT GTTGAGAGTC AGCAGTAGCC TCATCATCAC TAGATGGCAT
            421 TTCTTCTGAG CAAAACAGGT TTTCCTCATT AAAGGCATTC CACCACTGCT CCCATTCATC
            481 AGTTCCATAG GTTGGAATCT AAAATACACA AACAATTAGA ATCAGTAGTT TAACACATTA
            541 TACACTTAAA AATTTTATAT TTACCTTAGA GCTTTAAATC TCTGTAGGTA GTTTGTCCAA
            601 TTATGTCACA CCACAGAAGT AAGGTTCCTT CACAAAGATC CTCTAGCTAC CTAGCTAGCT
            661 GGCCAGGATC CCGTCGACGA ATTCGATATC CTTCTAGATT AGGACACCAC GGTTGCTGTG
            721 GACGATGTGA CACTGGTAGA ATTTGTGCTG GCATTTGTGT AATTCCCCTC GCTGTTTGTG
            781 TTTGACTCAT TGGAGGGCAC TTGGCTGGAG TTGGCTCTGA GAATCGCTCC TGCAGCGCTG
            841 CAGTGTGGCC TCGGCCCTGG CTCTGGTGTG TAGGTGAAGG TAAGGCTGGT GGAGTAAATG
            901 ACCCCGTCAT TTCGGACCAA AGTCACTGGA ACCTGGACTG GCTGGCGGAC CCATCTCCAA
            961 CCTTCCCGGA ATGCAGAAAT GTCTGGGACC ACACAGAGCA TGCTCTCTCC ACATCTGTAC
           1021 ATTGTTTCGG CTTTCTACAT CCCCAACCAC ACTCGTAAAT TTGGGAGTAA AGTTTTGTTC
           1081 CTGTAAGTCA GCATTGCTAC GTCCCCGACG CAATCACTGA GGACTTTCTA CGACAGGCAC
          1141 AAGGGGGTGA CTGGGGGGCC AAAGGGACAC C
          //
          Primer:
          Sequence file: RDB12303_A3Jmc.seq check
          >03023_12303_A3Jm_CMV-Forward_H06_24.ab1
              1 CGTCACTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
             61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC
            121 TCACTATAGG GAGACCCAAG CTTGCCACCA TGGCCCCCCC GACCGATGTC AGCCTGGGGG
            181 ACGAGCTCCA CTTAGACGGC GAGGACGTGG CGATGGCGCA TGCCGACGCG CTAGACGATT
            241 TCGATCTGGA CATGTTGGGG GACGGGGATT CCCCGGGTCC GGGATTTACC CCCCACGACT
            301 CCGCCCCCTA CGGCGCTCTG GATATGGCCG ACTTCGAGTT TGAGCAGATG TTTACCGATG
            361 CCCTTGGAAT TGACGAGTAC GGTGGGAAGC TTCTAGAATT CATGCCCTCC GGTTTTCCTC
            421 AGTCTCCACG TACGTCCCCG AGGGCGCGTC CCAAAACCCG GATAACCGGA GCGCTCCCCA
            481 TGGACTACTC GGAGGGCTTG TCCGCGGAGG AGCGGCCTGC GCATGCTCCA TCGGCGGGGA
            541 AGTTTGGTGA GCGGCCTCCA CCCAAACGAC TCACTAGGGA AGCTATGCGA AATTATTTAA
            601 AAGAACGAGG GGATCAAACA GTGCTCATTC TTCATGCAAA AGTTGCACAG AAGTCTTACG
          661 GAAATGAAAA A
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Kuroda, K., Delta-induced Notch signaling mediated by RBP-J inhibits MyoD expression and myogenesis. J. Biol. Chem., 274, 7238-7244 (1999). PMID 10066785.
          user report Shi, M., Forced notch signaling inhibits commissural axon outgrowth in the developing chick central nerve system. PLoS One, 6 (1): e14570 (2011). PMID 21283742.
          user report Blanpain C., Canonical notch signaling functions as a commitment switch in the epidermal lineage. Genes Dev., 20, 3022-3035 (2006). PMID 17079689.

          2022.02.09

          GNP_filter3_RDBDEP_html_220112.pl