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pCMX-N/VP16-RBP-J (#RDB03023)

Expression vector of mouse RBP-J

Alternative name pCMX-N/VP16-RBP-2
Clone info. Expression vector of constitutive active mouse RBP-J (VP16-RBP-J). Activation domain of VP16 was followed by N-terminal of RBP-J. Wild type (pCMX-N/RBP-J, RDB03021) is available.
Vector backbone pCMX-N/VP16 (Plasmid)
Size of vector backbone 4,785 bp
Selectable markers Amp^r
Gene/insert name Mouse RBP-J cDNA
Depositor|Developer Honjo, Tasuku | Kuroda, Kazuki |
 
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Terms and conditions for distribution For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Kyoto University Medical Science and Business Liaison Organization Liaison Office (contracts@kumbl.med.kyoto-u.ac.jp). Acknowledgement to Depositor in publication. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the name of resource, RECIPIENT's name and his/her affiliation, purpose of the use, and number of resources.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB03023 pCMX-N/VP16-RBP-J DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCMX-N/VP16-RBP-J was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB03023).

Reference section:

Kuroda, K., Tani, S., Tamura, K., Minoguchi, S., Kurooka, H., Honjo, T., Delta-induced Notch signaling mediated by RBP-J inhibits MyoD expression and myogenesis. J. Biol. Chem., 274, 7238-7244 (1999). PMID 10066785. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12303_A3Jm.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer:
Sequence file: RDB12303_A3Jma.seq check
>03023_12303_A3Jm_CMV-Forward_H06_24.ab1
    1 CGTCACTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC
  121 TCACTATAGG GAGACCCAAG CTTGCCACCA TGGCCCCCCC GACCGATGTC AGCCTGGGGG
  181 ACGAGCTCCA CTTAGACGGC GAGGACGTGG CGATGGCGCA TGCCGACGCG CTAGACGATT
  241 TCGATCTGGA CATGTTGGGG GACGGGGATT CCCCGGGTCC GGGATTTACC CCCCACGACT
  301 CCGCCCCCTA CGGCGCTCTG GATATGGCCG ACTTCGAGTT TGAGCAGATG TTTACCGATG
  361 CCCTTGGAAT TGACGAGTAC GGTGGGAAGC TTCTAGAATT CATGCCCTCC GGTTTTCCTC
  421 AGTCTCCACG TACGTCCCCG AGGGCGCGTC CCAAAACCCG GATAACCGGA GCGCTCCCCA
  481 TGGACTACTC GGAGGGCTTG TCCGCGGAGG AGCGGCCTGC GCATGCTCCA TCGGCGGGGA
  541 AGTTTGGTGA GCGGCCTCCA CCCAAACGAC TCACTAGGGA AGCTATGCGA AATTATTTAA
  601 AAGAACGAGG GGATCAAACA GTGCTCATTC TTCATGCAAA AGTTGCACAG AAGTCTTACG
  661 GAAATGAAAA ACGATTTTTT TGCCCTCCTC CTTGTGTGTA TCTTATGGGC AGTGGTTGGA
  721 AGAAAAAAAA AGAGCAAATG GAACGAGATG GATGTTCTGA ACAAGAGTCT CAACCCTGTG
  781 CGTTTATTGG AATAGGAAAC AGTGACCAAG AAATGCAGCA GCTGAACTTG GAAGGGAAGA
  841 ACTACTGCAC AGCCAAAACA TTGTACATAT CTGATTCAGA CAAGAGAAAA CATTTCATGT
  901 TGTCTGTAAA GATGTTCTAT GGCAACAGCG ATGACATTGG TGTGTTCCTC AGCAAGCGGA
  961 TAAAGGTCAT CTCCAAACCC TCCAAAAAGA AGCAGTCACT GAAGAATGCT GACTTGTGCA
 1021 TTGCTTCAGG AACGAAGTGG CACTGTTCAT CGCTTTCGGT CCCAGACAGT TAGTACCAGG
 1081 TACTGCATGT AGAAGGAGGG AATTCACGCA AGTTCACACC AGCTGGGGAG CCATTTTAAC
1141 ATCCAATCTC TCTTTGGACC GAACGAG
//
Primer:
Sequence file: RDB12303_A3Jmb.seq check
>03023_12303_A3Jm_pAxEFR2_E01_13.ab1
    1 CGTGTCAAAT GCTTTATTTG TGAATTTGTG ATGCTATTGC TTTATTTGTA ACCATTATAA
   61 GCTGCAATAA ACAAGTTAAC AACAACAATT GCATTCATTT TATGTTTCAG GTTCAGGGGG
  121 AGGTGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGGTATG GCTGATTATG
  181 ATCTCTAGTC AAGGCACTAT ACATCAAATA TTTTTCCATA ATTTTCTTGT ATAGCAGTGC
  241 AGCTTTTTCC TTTGTGGTGT AAATAGCAAA GCAAGCAAGA GTTCTATTAC TAAACACAGC
  301 ATGACTCAAA AAACTTAGCA ATTCTGAAGG AAAGTCCTTG GGGTCTTCTA CCTTTCTCTT
  361 CTTTTTTGGA GGAGTAGAAT GTTGAGAGTC AGCAGTAGCC TCATCATCAC TAGATGGCAT
  421 TTCTTCTGAG CAAAACAGGT TTTCCTCATT AAAGGCATTC CACCACTGCT CCCATTCATC
  481 AGTTCCATAG GTTGGAATCT AAAATACACA AACAATTAGA ATCAGTAGTT TAACACATTA
  541 TACACTTAAA AATTTTATAT TTACCTTAGA GCTTTAAATC TCTGTAGGTA GTTTGTCCAA
  601 TTATGTCACA CCACAGAAGT AAGGTTCCTT CACAAAGATC CTCTAGCTAC CTAGCTAGCT
  661 GGCCAGGATC CCGTCGACGA ATTCGATATC CTTCTAGATT AGGACACCAC GGTTGCTGTG
  721 GACGATGTGA CACTGGTAGA ATTTGTGCTG GCATTTGTGT AATTCCCCTC GCTGTTTGTG
  781 TTTGACTCAT TGGAGGGCAC TTGGCTGGAG TTGGCTCTGA GAATCGCTCC TGCAGCGCTG
  841 CAGTGTGGCC TCGGCCCTGG CTCTGGTGTG TAGGTGAAGG TAAGGCTGGT GGAGTAAATG
  901 ACCCCGTCAT TTCGGACCAA AGTCACTGGA ACCTGGACTG GCTGGCGGAC CCATCTCCAA
  961 CCTTCCCGGA ATGCAGAAAT GTCTGGGACC ACACAGAGCA TGCTCTCTCC ACATCTGTAC
 1021 ATTGTTTCGG CTTTCTACAT CCCCAACCAC ACTCGTAAAT TTGGGAGTAA AGTTTTGTTC
 1081 CTGTAAGTCA GCATTGCTAC GTCCCCGACG CAATCACTGA GGACTTTCTA CGACAGGCAC
1141 AAGGGGGTGA CTGGGGGGCC AAAGGGACAC C
//
Primer:
Sequence file: RDB12303_A3Jmc.seq check
>03023_12303_A3Jm_CMV-Forward_H06_24.ab1
    1 CGTCACTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC
  121 TCACTATAGG GAGACCCAAG CTTGCCACCA TGGCCCCCCC GACCGATGTC AGCCTGGGGG
  181 ACGAGCTCCA CTTAGACGGC GAGGACGTGG CGATGGCGCA TGCCGACGCG CTAGACGATT
  241 TCGATCTGGA CATGTTGGGG GACGGGGATT CCCCGGGTCC GGGATTTACC CCCCACGACT
  301 CCGCCCCCTA CGGCGCTCTG GATATGGCCG ACTTCGAGTT TGAGCAGATG TTTACCGATG
  361 CCCTTGGAAT TGACGAGTAC GGTGGGAAGC TTCTAGAATT CATGCCCTCC GGTTTTCCTC
  421 AGTCTCCACG TACGTCCCCG AGGGCGCGTC CCAAAACCCG GATAACCGGA GCGCTCCCCA
  481 TGGACTACTC GGAGGGCTTG TCCGCGGAGG AGCGGCCTGC GCATGCTCCA TCGGCGGGGA
  541 AGTTTGGTGA GCGGCCTCCA CCCAAACGAC TCACTAGGGA AGCTATGCGA AATTATTTAA
  601 AAGAACGAGG GGATCAAACA GTGCTCATTC TTCATGCAAA AGTTGCACAG AAGTCTTACG
661 GAAATGAAAA A
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kuroda, K., Delta-induced Notch signaling mediated by RBP-J inhibits MyoD expression and myogenesis. J. Biol. Chem., 274, 7238-7244 (1999). PMID 10066785. [link to RRC of NBRP]
user_report Shi, M., Forced notch signaling inhibits commissural axon outgrowth in the developing chick central nerve system. PLoS One, 6 (1): e14570 (2011). PMID 21283742. [link to RRC of NBRP]
user_report Blanpain C., Canonical notch signaling functions as a commitment switch in the epidermal lineage. Gene. Dev., 20, 3022-3035 (2006). PMID 17079689. [link to RRC of NBRP]