Resource data sheet
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pCMX-N/VP16-RBP-J

Expression vector of mouse RBP-J

Catalog number RDB03023
Resource name pCMX-N/VP16-RBP-J
Alternative name pCMX-N/VP16-RBP-2
Clone info. Expression vector of constitutive active mouse RBP-J (VP16-RBP-J). Activation domain of VP16 was followed by N-terminal of RBP-J. Wild type (pCMX-N/RBP-J, RDB03021) is available.
Vector backbone pCMX-N/VP16 (Plasmid)
Size of vector backbone 4,785 bp
Selectable markers Amp^r
Gene/insert name Mouse RBP-J cDNA
Depositor Honjo, Tasuku | Kuroda, Kazuki |
 
Other clones in our bank mouse Rbpj (NCBI Gene 19664) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Kyoto University Medical Science and Business Liaison Organization Liaison Office (contracts@kumbl.med.kyoto-u.ac.jp). Acknowledgement to Depositor in publication. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the name of resource, RECIPIENT's name and his/her affiliation, purpose of the use, and number of resources.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB03023 pCMX-N/VP16-RBP-J DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Map RDB03023.gif from Depositor

Featured content

References

original Kuroda, K., Delta-induced Notch signaling mediated by RBP-J inhibits MyoD expression and myogenesis. J. Biol. Chem., 274, 7238-7244 (1999). PMID 10066785.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12303_A3Jm.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: RDB12303_A3Jma.seq
>03023_12303_A3Jm_CMV-Forward_H06_24.ab1
    1 CGTCACTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC
  121 TCACTATAGG GAGACCCAAG CTTGCCACCA TGGCCCCCCC GACCGATGTC AGCCTGGGGG
  181 ACGAGCTCCA CTTAGACGGC GAGGACGTGG CGATGGCGCA TGCCGACGCG CTAGACGATT
  241 TCGATCTGGA CATGTTGGGG GACGGGGATT CCCCGGGTCC GGGATTTACC CCCCACGACT
  301 CCGCCCCCTA CGGCGCTCTG GATATGGCCG ACTTCGAGTT TGAGCAGATG TTTACCGATG
  361 CCCTTGGAAT TGACGAGTAC GGTGGGAAGC TTCTAGAATT CATGCCCTCC GGTTTTCCTC
  421 AGTCTCCACG TACGTCCCCG AGGGCGCGTC CCAAAACCCG GATAACCGGA GCGCTCCCCA
  481 TGGACTACTC GGAGGGCTTG TCCGCGGAGG AGCGGCCTGC GCATGCTCCA TCGGCGGGGA
  541 AGTTTGGTGA GCGGCCTCCA CCCAAACGAC TCACTAGGGA AGCTATGCGA AATTATTTAA
  601 AAGAACGAGG GGATCAAACA GTGCTCATTC TTCATGCAAA AGTTGCACAG AAGTCTTACG
  661 GAAATGAAAA ACGATTTTTT TGCCCTCCTC CTTGTGTGTA TCTTATGGGC AGTGGTTGGA
  721 AGAAAAAAAA AGAGCAAATG GAACGAGATG GATGTTCTGA ACAAGAGTCT CAACCCTGTG
  781 CGTTTATTGG AATAGGAAAC AGTGACCAAG AAATGCAGCA GCTGAACTTG GAAGGGAAGA
  841 ACTACTGCAC AGCCAAAACA TTGTACATAT CTGATTCAGA CAAGAGAAAA CATTTCATGT
  901 TGTCTGTAAA GATGTTCTAT GGCAACAGCG ATGACATTGG TGTGTTCCTC AGCAAGCGGA
  961 TAAAGGTCAT CTCCAAACCC TCCAAAAAGA AGCAGTCACT GAAGAATGCT GACTTGTGCA
 1021 TTGCTTCAGG AACGAAGTGG CACTGTTCAT CGCTTTCGGT CCCAGACAGT TAGTACCAGG
 1081 TACTGCATGT AGAAGGAGGG AATTCACGCA AGTTCACACC AGCTGGGGAG CCATTTTAAC
1141 ATCCAATCTC TCTTTGGACC GAACGAG
//
Primer: RDB12303_A3Jmb.seq
>03023_12303_A3Jm_pAxEFR2_E01_13.ab1
    1 CGTGTCAAAT GCTTTATTTG TGAATTTGTG ATGCTATTGC TTTATTTGTA ACCATTATAA
   61 GCTGCAATAA ACAAGTTAAC AACAACAATT GCATTCATTT TATGTTTCAG GTTCAGGGGG
  121 AGGTGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGGTATG GCTGATTATG
  181 ATCTCTAGTC AAGGCACTAT ACATCAAATA TTTTTCCATA ATTTTCTTGT ATAGCAGTGC
  241 AGCTTTTTCC TTTGTGGTGT AAATAGCAAA GCAAGCAAGA GTTCTATTAC TAAACACAGC
  301 ATGACTCAAA AAACTTAGCA ATTCTGAAGG AAAGTCCTTG GGGTCTTCTA CCTTTCTCTT
  361 CTTTTTTGGA GGAGTAGAAT GTTGAGAGTC AGCAGTAGCC TCATCATCAC TAGATGGCAT
  421 TTCTTCTGAG CAAAACAGGT TTTCCTCATT AAAGGCATTC CACCACTGCT CCCATTCATC
  481 AGTTCCATAG GTTGGAATCT AAAATACACA AACAATTAGA ATCAGTAGTT TAACACATTA
  541 TACACTTAAA AATTTTATAT TTACCTTAGA GCTTTAAATC TCTGTAGGTA GTTTGTCCAA
  601 TTATGTCACA CCACAGAAGT AAGGTTCCTT CACAAAGATC CTCTAGCTAC CTAGCTAGCT
  661 GGCCAGGATC CCGTCGACGA ATTCGATATC CTTCTAGATT AGGACACCAC GGTTGCTGTG
  721 GACGATGTGA CACTGGTAGA ATTTGTGCTG GCATTTGTGT AATTCCCCTC GCTGTTTGTG
  781 TTTGACTCAT TGGAGGGCAC TTGGCTGGAG TTGGCTCTGA GAATCGCTCC TGCAGCGCTG
  841 CAGTGTGGCC TCGGCCCTGG CTCTGGTGTG TAGGTGAAGG TAAGGCTGGT GGAGTAAATG
  901 ACCCCGTCAT TTCGGACCAA AGTCACTGGA ACCTGGACTG GCTGGCGGAC CCATCTCCAA
  961 CCTTCCCGGA ATGCAGAAAT GTCTGGGACC ACACAGAGCA TGCTCTCTCC ACATCTGTAC
 1021 ATTGTTTCGG CTTTCTACAT CCCCAACCAC ACTCGTAAAT TTGGGAGTAA AGTTTTGTTC
 1081 CTGTAAGTCA GCATTGCTAC GTCCCCGACG CAATCACTGA GGACTTTCTA CGACAGGCAC
1141 AAGGGGGTGA CTGGGGGGCC AAAGGGACAC C
//
Primer: RDB12303_A3Jmc.seq
>03023_12303_A3Jm_CMV-Forward_H06_24.ab1
    1 CGTCACTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC
  121 TCACTATAGG GAGACCCAAG CTTGCCACCA TGGCCCCCCC GACCGATGTC AGCCTGGGGG
  181 ACGAGCTCCA CTTAGACGGC GAGGACGTGG CGATGGCGCA TGCCGACGCG CTAGACGATT
  241 TCGATCTGGA CATGTTGGGG GACGGGGATT CCCCGGGTCC GGGATTTACC CCCCACGACT
  301 CCGCCCCCTA CGGCGCTCTG GATATGGCCG ACTTCGAGTT TGAGCAGATG TTTACCGATG
  361 CCCTTGGAAT TGACGAGTAC GGTGGGAAGC TTCTAGAATT CATGCCCTCC GGTTTTCCTC
  421 AGTCTCCACG TACGTCCCCG AGGGCGCGTC CCAAAACCCG GATAACCGGA GCGCTCCCCA
  481 TGGACTACTC GGAGGGCTTG TCCGCGGAGG AGCGGCCTGC GCATGCTCCA TCGGCGGGGA
  541 AGTTTGGTGA GCGGCCTCCA CCCAAACGAC TCACTAGGGA AGCTATGCGA AATTATTTAA
  601 AAGAACGAGG GGATCAAACA GTGCTCATTC TTCATGCAAA AGTTGCACAG AAGTCTTACG
661 GAAATGAAAA A
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Kuroda, K., Delta-induced Notch signaling mediated by RBP-J inhibits MyoD expression and myogenesis. J. Biol. Chem., 274, 7238-7244 (1999). PMID 10066785.
user report Shi, M., Forced notch signaling inhibits commissural axon outgrowth in the developing chick central nerve system. PLoS One, 6 (1): e14570 (2011). PMID 21283742.
user report Blanpain C., Canonical notch signaling functions as a commitment switch in the epidermal lineage. Genes Dev., 20, 3022-3035 (2006). PMID 17079689.

2019.02.05

GNP_filter3_RDBDEP_html_190131.pl