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pCMX-N/RBP-J (R218H) (#RDB03022)

Expression vector of mouse RBP-J

Alternative name pCMX-N/RBP-2 (R218H)
Clone info. Expression vector of dominant negative form of mouse RBP-J (R218H) under the control of CMV promoter. Wild type (pCMX-N/RBP-J, RDB03021) is available.
Vector backbone pCMX-N (Plasmid)
Size of vector backbone 4,536 bp
Selectable markers Amp^r
Gene/insert name Mouse RBP-J cDNA
Depositor|Developer Honjo, Tasuku | Kuroda, Kazuki |
 
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Terms and conditions for distribution For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Kyoto University Medical Science and Business Liaison Organization Liaison Office (contracts@kumbl.med.kyoto-u.ac.jp). Acknowledgement to Depositor in publication. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the name of resource, RECIPIENT's name and his/her affiliation, purpose of the use, and number of resources.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB03022 pCMX-N/RBP-J (R218H) DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCMX-N/RBP-J (R218H) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB03022).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12302_A3Jm.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer:
Sequence file: RDB12302_A3Jma.seq check
>03022_12302_A3Jm_T7-20bp_E10_13.ab1
    1 CCTAAGGTCT CTAGACGATA TCGAATTCCT GCAGCCCAAT ATGCCCTCCG GTTTTCCTCA
   61 GTCTCCACGT ACGTCCCCGA GGGCGCGTCC CAAAACCCGG ATAACCGGAG CGCTCCCCAT
  121 GGACTACTCG GAGGGCTTGT CCGCGGAGGA GCGGCCTGCG CATGCTCCAT CGGCGGGGAA
  181 GTTTGGTGAG CGGCCTCCAC CCAAACGACT CACTAGGGAA GCTATGCGAA ATTATTTAAA
  241 AGAACGAGGG GATCAAACAG TGCTCATTCT TCATGCAAAA GTTGCACAGA AGTCTTACGG
  301 AAATGAAAAA CGATTTTTTT GCCCTCCTCC TTGTGTGTAT CTTATGGGCA GTGGTTGGAA
  361 GAAAAAAAAA GAGCAAATGG AACGAGATGG ATGTTCTGAA CAAGAGTCTC AACCCTGTGC
  421 GTTTATTGGA ATAGGAAACA GTGACCAAGA AATGCAGCAG CTGAACCTCG AGGGGAAGAA
  481 CTACTGCACA GCCAAAACAT TGTACATATC TGATTCAGAC AAGAGAAAAC ATTTCATGTT
  541 GTCTGTAAAG ATGTTCTATG GCAACAGCGA TGACATTGGT GTGTTCCTCA GCAAGCGGAT
  601 AAAGGTCATC TCCAAACCCT CCAAAAAGAA GCAGTCACTG AAGAATGCTG ACTTGTGCAT
  661 TGCTTCAGGA ACGAAGGTTG CACTGTTCAA TCACCTTAGA TCTCAGACAG TTAGTACCAG
  721 GTACCTGCAT GTAGAAGGAG GGAATTTCCA CGCCAGTTCA CAACAGTGGG GAGCATTTTA
  781 CATCCATCTC TTGGACGACG ACGAGTCGGA AGGAGAGGAG TTCACAGTTA GAGATGGCTA
  841 CATCCATTAC GGGCAGACTG TCAAGCTTGT GTGCTCAGTG ACTGGCATGG CACTCCCAAG
  901 ATTGATAATT AGGAAAGTTG ATAAGCAGAC GGCATTACTG GATGCAGACG ACCCTGTATC
  961 ACAACTCCAC AAATGTGCAT TTTACCTTAA GGATACAGAA AGAATGTACT TGTGCCTTTC
 1021 TCAGAAAGAA TAATCCAATT TCAGGCCACT CCATGTCCAA AGAACAAAAA TAAGGAAAAT
 1081 GAATAACGAA TGGAGCTTCC TGGACATCAT AGCACAGACC AGCCAGATAC ACGTTTCTTA
1141 TTGGAGGGAA TTGGGGCCCC GGATTCT
//
Primer:
Sequence file: RDB12302_A3Jmb.seq check
>03022_12302_A3Jm_pAxEFR2_C01_07.ab1
    1 CAGGGGAAAA TGCTTTATTT GTGAATTTGT GATGCTATTG CTTTATTTGT AACCATTATA
   61 AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT TTATGTTTCA GGTTCAGGGG
  121 GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAT GGCTGATTAT
  181 GATCTCTAGT CAAGGCACTA TACATCAAAT ATTTTTCCAT AATTTTCTTG TATAGCAGTG
  241 CAGCTTTTTC CTTTGTGGTG TAAATAGCAA AGCAAGCAAG AGTTCTATTA CTAAACACAG
  301 CATGACTCAA AAAACTTAGC AATTCTGAAG GAAAGTCCTT GGGGTCTTCT ACCTTTCTCT
  361 TCTTTTTTGG AGGAGTAGAA TGTTGAGAGT CAGCAGTAGC CTCATCATCA CTAGATGGCA
  421 TTTCTTCTGA GCAAAACAGG TTTTCCTCAT TAAAGGCATT CCACCACTGC TCCCATTCAT
  481 CAGTTCCATA GGTTGGAATC TAAAATACAC AAACAATTAG AATCAGTAGT TTAACACATT
  541 ATACACTTAA AAATTTTATA TTTACCTTAG AGCTTTAAAT CTCTGTAGGT AGTTTGTCCA
  601 ATTATGTCAC ACCACAGAAG TAAGGTTCCT TCACAAAGAT CCTCTAGCTA CCTAGCTAGC
  661 TGGCCAGGAT CCCGTCGACG GATCCCCAAC TTTTTGCTGC ATTCAAGTCA GTTTCAGTCC
  721 CAGCAAAAAG ACGGTAGTTA GGACACCACG GTTGCTGTGG ACGATGTGAC ACTGGTAGAA
  781 TTTGTGCTGG CATTTGTGTA ATTCCCCTCG CTGTTTGTGT TTGACTCATT GGAGGGCACT
  841 TGGCTGGAGT TGGCTCTGAG AATCGCTCCT GCAGCGCTGC AGTGTGGCCT CGGCCCTGGC
  901 TCTGGTGTGT AGGTGAAGGT AAGGCTGGTG GAGTAAATGA CCCCGTCATT TCGGACCAAA
  961 GTCACTGGAA CCTGGACTGG CTGGCGGACC CATCTCCAAC CTTCCCGGAA TGCAGAAATG
 1021 TCTGGGACCA CACAGAGCAT GCTCTCTCCA CATCTGTACA TTGTTTCGGC TTCTACATCC
 1081 CCAACCACAC TCGTAATTTG GGAGTAAAGT TGGTCTGGTA AGGTCAGCAA TGCTACGTCC
1141 CCGCCCGCCA TTCAACCTGG AAGGAACCTT TTCTTC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

user_report Shi, M., Forced notch signaling inhibits commissural axon outgrowth in the developing chick central nerve system. PLoS One, 6 (1): e14570 (2011). PMID 21283742. [link to RRC of NBRP]
user_report Kacer, D., Regulation of nonclassical fgf1 release and fgf-dependent cell transformation by CBF1-mediated notch signaling. J. Cell. Physiol., 226 (11): 3064-3075 (2011). PMID 21302306. [link to RRC of NBRP]
user_report Lee, Y.J., Involvement of Notch1 inhibition in serum-stimulated glia and oligodendrocyte differentiation from human mesenchymal stem cells. Stem Cells and Cloning: Advances and Applications, 3: 165-173 (2010). PMID 24198522. [link to RRC of NBRP]
user_report Limbourg A., Notch Ligand Delta-Like 1 Is Essential for Postnatal Arteriogenesis. Circ. Res., 100, 363-371 (2007). PMID 17234965. [link to RRC of NBRP]
user_report Ossipova, O., PAR1 specifies ciliated cells in vertebrate ectoderm downstream of aPKC. Development, 134 (23), 4297-4306 (2007). PMID 17993468. [link to RRC of NBRP]
user_report Blanpain C., Canonical notch signaling functions as a commitment switch in the epidermal lineage. Gene. Dev., 20, 3022-3035 (2006). PMID 17079689. [link to RRC of NBRP]