Expression vector of mouse RBP-J
| Catalog number | RDB03022 |
|---|---|
| Resource name | pCMX-N/RBP-J (R218H) |
| Alternative name | pCMX-N/RBP-2 (R218H) |
| Clone info. | Expression vector of dominant negative form of mouse RBP-J (R218H) under the control of CMV promoter. Wild type (pCMX-N/RBP-J, RDB03021) is available. |
| Vector backbone | pCMX-N (Plasmid) |
| Size of vector backbone | 4,536 bp |
| Selectable markers | Amp^r |
| Gene/insert name | Mouse RBP-J cDNA |
| Depositor | Honjo, Tasuku | Kuroda, Kazuki | |
| Other clones in our bank | mouse Rbpj (NCBI Gene 19664) | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Kyoto University Medical Science and Business Liaison Organization Liaison Office (contracts@phy.med.kyoto-u.ac.jp). Acknowledgement to Depositor in publication. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the name of resource, RECIPIENT's name and his/her affiliation, purpose of the use, and number of resources. |
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| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB03022 | pCMX-N/RBP-J (R218H) | DNA solution |
Electronic file
| Electronic file | Map RDB03022.gif from Depositor
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Featured content
| Featured content | Dnaconda's recommendation EXR0042e (English text) |
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| Featured content | Dnaconda's recommendation EXR0042j (Japanese text) |
References
User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB12302_A3Jm.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: | RDB12302_A3Jma.seq |
|---|---|
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>03022_12302_A3Jm_T7-20bp_E10_13.ab1 1 CCTAAGGTCT CTAGACGATA TCGAATTCCT GCAGCCCAAT ATGCCCTCCG GTTTTCCTCA 61 GTCTCCACGT ACGTCCCCGA GGGCGCGTCC CAAAACCCGG ATAACCGGAG CGCTCCCCAT 121 GGACTACTCG GAGGGCTTGT CCGCGGAGGA GCGGCCTGCG CATGCTCCAT CGGCGGGGAA 181 GTTTGGTGAG CGGCCTCCAC CCAAACGACT CACTAGGGAA GCTATGCGAA ATTATTTAAA 241 AGAACGAGGG GATCAAACAG TGCTCATTCT TCATGCAAAA GTTGCACAGA AGTCTTACGG 301 AAATGAAAAA CGATTTTTTT GCCCTCCTCC TTGTGTGTAT CTTATGGGCA GTGGTTGGAA 361 GAAAAAAAAA GAGCAAATGG AACGAGATGG ATGTTCTGAA CAAGAGTCTC AACCCTGTGC 421 GTTTATTGGA ATAGGAAACA GTGACCAAGA AATGCAGCAG CTGAACCTCG AGGGGAAGAA 481 CTACTGCACA GCCAAAACAT TGTACATATC TGATTCAGAC AAGAGAAAAC ATTTCATGTT 541 GTCTGTAAAG ATGTTCTATG GCAACAGCGA TGACATTGGT GTGTTCCTCA GCAAGCGGAT 601 AAAGGTCATC TCCAAACCCT CCAAAAAGAA GCAGTCACTG AAGAATGCTG ACTTGTGCAT 661 TGCTTCAGGA ACGAAGGTTG CACTGTTCAA TCACCTTAGA TCTCAGACAG TTAGTACCAG 721 GTACCTGCAT GTAGAAGGAG GGAATTTCCA CGCCAGTTCA CAACAGTGGG GAGCATTTTA 781 CATCCATCTC TTGGACGACG ACGAGTCGGA AGGAGAGGAG TTCACAGTTA GAGATGGCTA 841 CATCCATTAC GGGCAGACTG TCAAGCTTGT GTGCTCAGTG ACTGGCATGG CACTCCCAAG 901 ATTGATAATT AGGAAAGTTG ATAAGCAGAC GGCATTACTG GATGCAGACG ACCCTGTATC 961 ACAACTCCAC AAATGTGCAT TTTACCTTAA GGATACAGAA AGAATGTACT TGTGCCTTTC 1021 TCAGAAAGAA TAATCCAATT TCAGGCCACT CCATGTCCAA AGAACAAAAA TAAGGAAAAT 1081 GAATAACGAA TGGAGCTTCC TGGACATCAT AGCACAGACC AGCCAGATAC ACGTTTCTTA 1141 TTGGAGGGAA TTGGGGCCCC GGATTCT // |
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| Primer: | RDB12302_A3Jmb.seq |
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>03022_12302_A3Jm_pAxEFR2_C01_07.ab1 1 CAGGGGAAAA TGCTTTATTT GTGAATTTGT GATGCTATTG CTTTATTTGT AACCATTATA 61 AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT TTATGTTTCA GGTTCAGGGG 121 GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAT GGCTGATTAT 181 GATCTCTAGT CAAGGCACTA TACATCAAAT ATTTTTCCAT AATTTTCTTG TATAGCAGTG 241 CAGCTTTTTC CTTTGTGGTG TAAATAGCAA AGCAAGCAAG AGTTCTATTA CTAAACACAG 301 CATGACTCAA AAAACTTAGC AATTCTGAAG GAAAGTCCTT GGGGTCTTCT ACCTTTCTCT 361 TCTTTTTTGG AGGAGTAGAA TGTTGAGAGT CAGCAGTAGC CTCATCATCA CTAGATGGCA 421 TTTCTTCTGA GCAAAACAGG TTTTCCTCAT TAAAGGCATT CCACCACTGC TCCCATTCAT 481 CAGTTCCATA GGTTGGAATC TAAAATACAC AAACAATTAG AATCAGTAGT TTAACACATT 541 ATACACTTAA AAATTTTATA TTTACCTTAG AGCTTTAAAT CTCTGTAGGT AGTTTGTCCA 601 ATTATGTCAC ACCACAGAAG TAAGGTTCCT TCACAAAGAT CCTCTAGCTA CCTAGCTAGC 661 TGGCCAGGAT CCCGTCGACG GATCCCCAAC TTTTTGCTGC ATTCAAGTCA GTTTCAGTCC 721 CAGCAAAAAG ACGGTAGTTA GGACACCACG GTTGCTGTGG ACGATGTGAC ACTGGTAGAA 781 TTTGTGCTGG CATTTGTGTA ATTCCCCTCG CTGTTTGTGT TTGACTCATT GGAGGGCACT 841 TGGCTGGAGT TGGCTCTGAG AATCGCTCCT GCAGCGCTGC AGTGTGGCCT CGGCCCTGGC 901 TCTGGTGTGT AGGTGAAGGT AAGGCTGGTG GAGTAAATGA CCCCGTCATT TCGGACCAAA 961 GTCACTGGAA CCTGGACTGG CTGGCGGACC CATCTCCAAC CTTCCCGGAA TGCAGAAATG 1021 TCTGGGACCA CACAGAGCAT GCTCTCTCCA CATCTGTACA TTGTTTCGGC TTCTACATCC 1081 CCAACCACAC TCGTAATTTG GGAGTAAAGT TGGTCTGGTA AGGTCAGCAA TGCTACGTCC 1141 CCGCCCGCCA TTCAACCTGG AAGGAACCTT TTCTTC // |
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Sequencing and PCR primers for primer information.
References
2018.12.11
GNP_filter3_RDBDEP_html_181012.pl