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pCMX-N/RBP-J (R218H)

Expression vector of mouse RBP-J

Catalog number RDB03022
Resource name pCMX-N/RBP-J (R218H)
Alternative name pCMX-N/RBP-2 (R218H)
Clone info. Expression vector of dominant negative form of mouse RBP-J (R218H) under the control of CMV promoter. Wild type (pCMX-N/RBP-J, RDB03021) is available.
Vector backbone pCMX-N (Plasmid)
Size of vector backbone 4,536 bp
Selectable markers Amp^r
Gene/insert name Mouse RBP-J cDNA
Depositor|Developer Honjo, Tasuku | Kuroda, Kazuki |
Other clones in our bank

External Database
Mouse RBP-J

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Kyoto University Medical Science and Business Liaison Organization Liaison Office (contracts@kumbl.med.kyoto-u.ac.jp). Acknowledgement to Depositor in publication. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the name of resource, RECIPIENT's name and his/her affiliation, purpose of the use, and number of resources.
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          提供条件 営利機関の利用者は、事前に京都大学・「医学領域」産学連携推進機構・知財室 (contracts@kumbl.med.kyoto-u.ac.jp) に連絡し、承諾を得ること。研究結果の発表時、謝辞に寄託者名を入れ ること。理研BRCは、本件リソースのリソース名、提供先、利用目的、件数を京都大学・本庶佑教授に報告する。
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          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB03022 pCMX-N/RBP-J (R218H) DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (gif) RDB03022.gif

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Dnaconda's recommendation EXR0042e (English text)
          Featured content Dnaconda's recommendation EXR0042j (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB12302_A3Jm.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer:
          Sequence file: RDB12302_A3Jma.seq check
          >03022_12302_A3Jm_T7-20bp_E10_13.ab1
              1 CCTAAGGTCT CTAGACGATA TCGAATTCCT GCAGCCCAAT ATGCCCTCCG GTTTTCCTCA
             61 GTCTCCACGT ACGTCCCCGA GGGCGCGTCC CAAAACCCGG ATAACCGGAG CGCTCCCCAT
            121 GGACTACTCG GAGGGCTTGT CCGCGGAGGA GCGGCCTGCG CATGCTCCAT CGGCGGGGAA
            181 GTTTGGTGAG CGGCCTCCAC CCAAACGACT CACTAGGGAA GCTATGCGAA ATTATTTAAA
            241 AGAACGAGGG GATCAAACAG TGCTCATTCT TCATGCAAAA GTTGCACAGA AGTCTTACGG
            301 AAATGAAAAA CGATTTTTTT GCCCTCCTCC TTGTGTGTAT CTTATGGGCA GTGGTTGGAA
            361 GAAAAAAAAA GAGCAAATGG AACGAGATGG ATGTTCTGAA CAAGAGTCTC AACCCTGTGC
            421 GTTTATTGGA ATAGGAAACA GTGACCAAGA AATGCAGCAG CTGAACCTCG AGGGGAAGAA
            481 CTACTGCACA GCCAAAACAT TGTACATATC TGATTCAGAC AAGAGAAAAC ATTTCATGTT
            541 GTCTGTAAAG ATGTTCTATG GCAACAGCGA TGACATTGGT GTGTTCCTCA GCAAGCGGAT
            601 AAAGGTCATC TCCAAACCCT CCAAAAAGAA GCAGTCACTG AAGAATGCTG ACTTGTGCAT
            661 TGCTTCAGGA ACGAAGGTTG CACTGTTCAA TCACCTTAGA TCTCAGACAG TTAGTACCAG
            721 GTACCTGCAT GTAGAAGGAG GGAATTTCCA CGCCAGTTCA CAACAGTGGG GAGCATTTTA
            781 CATCCATCTC TTGGACGACG ACGAGTCGGA AGGAGAGGAG TTCACAGTTA GAGATGGCTA
            841 CATCCATTAC GGGCAGACTG TCAAGCTTGT GTGCTCAGTG ACTGGCATGG CACTCCCAAG
            901 ATTGATAATT AGGAAAGTTG ATAAGCAGAC GGCATTACTG GATGCAGACG ACCCTGTATC
            961 ACAACTCCAC AAATGTGCAT TTTACCTTAA GGATACAGAA AGAATGTACT TGTGCCTTTC
           1021 TCAGAAAGAA TAATCCAATT TCAGGCCACT CCATGTCCAA AGAACAAAAA TAAGGAAAAT
           1081 GAATAACGAA TGGAGCTTCC TGGACATCAT AGCACAGACC AGCCAGATAC ACGTTTCTTA
          1141 TTGGAGGGAA TTGGGGCCCC GGATTCT
          //
          Primer:
          Sequence file: RDB12302_A3Jmb.seq check
          >03022_12302_A3Jm_pAxEFR2_C01_07.ab1
              1 CAGGGGAAAA TGCTTTATTT GTGAATTTGT GATGCTATTG CTTTATTTGT AACCATTATA
             61 AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT TTATGTTTCA GGTTCAGGGG
            121 GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAT GGCTGATTAT
            181 GATCTCTAGT CAAGGCACTA TACATCAAAT ATTTTTCCAT AATTTTCTTG TATAGCAGTG
            241 CAGCTTTTTC CTTTGTGGTG TAAATAGCAA AGCAAGCAAG AGTTCTATTA CTAAACACAG
            301 CATGACTCAA AAAACTTAGC AATTCTGAAG GAAAGTCCTT GGGGTCTTCT ACCTTTCTCT
            361 TCTTTTTTGG AGGAGTAGAA TGTTGAGAGT CAGCAGTAGC CTCATCATCA CTAGATGGCA
            421 TTTCTTCTGA GCAAAACAGG TTTTCCTCAT TAAAGGCATT CCACCACTGC TCCCATTCAT
            481 CAGTTCCATA GGTTGGAATC TAAAATACAC AAACAATTAG AATCAGTAGT TTAACACATT
            541 ATACACTTAA AAATTTTATA TTTACCTTAG AGCTTTAAAT CTCTGTAGGT AGTTTGTCCA
            601 ATTATGTCAC ACCACAGAAG TAAGGTTCCT TCACAAAGAT CCTCTAGCTA CCTAGCTAGC
            661 TGGCCAGGAT CCCGTCGACG GATCCCCAAC TTTTTGCTGC ATTCAAGTCA GTTTCAGTCC
            721 CAGCAAAAAG ACGGTAGTTA GGACACCACG GTTGCTGTGG ACGATGTGAC ACTGGTAGAA
            781 TTTGTGCTGG CATTTGTGTA ATTCCCCTCG CTGTTTGTGT TTGACTCATT GGAGGGCACT
            841 TGGCTGGAGT TGGCTCTGAG AATCGCTCCT GCAGCGCTGC AGTGTGGCCT CGGCCCTGGC
            901 TCTGGTGTGT AGGTGAAGGT AAGGCTGGTG GAGTAAATGA CCCCGTCATT TCGGACCAAA
            961 GTCACTGGAA CCTGGACTGG CTGGCGGACC CATCTCCAAC CTTCCCGGAA TGCAGAAATG
           1021 TCTGGGACCA CACAGAGCAT GCTCTCTCCA CATCTGTACA TTGTTTCGGC TTCTACATCC
           1081 CCAACCACAC TCGTAATTTG GGAGTAAAGT TGGTCTGGTA AGGTCAGCAA TGCTACGTCC
          1141 CCGCCCGCCA TTCAACCTGG AAGGAACCTT TTCTTC
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          user report Shi, M., Forced notch signaling inhibits commissural axon outgrowth in the developing chick central nerve system. PLoS One, 6 (1): e14570 (2011). PMID 21283742.
          user report Kacer, D., Regulation of nonclassical fgf1 release and fgf-dependent cell transformation by CBF1-mediated notch signaling. J. Cell. Physiol., 226 (11): 3064-3075 (2011). PMID 21302306.
          user report Lee, Y.J., Involvement of Notch1 inhibition in serum-stimulated glia and oligodendrocyte differentiation from human mesenchymal stem cells. Stem Cells and Cloning: Advances and Applications, 3: 165-173 (2010). PMID 24198522.
          user report Limbourg A., Notch Ligand Delta-Like 1 Is Essential for Postnatal Arteriogenesis. Circ. Res., 100, 363-371 (2007). PMID 17234965.
          user report Ossipova, O., PAR1 specifies ciliated cells in vertebrate ectoderm downstream of aPKC. Development, 134 (23), 4297-4306 (2007). PMID 17993468.
          user report Blanpain C., Canonical notch signaling functions as a commitment switch in the epidermal lineage. Genes Dev., 20, 3022-3035 (2006). PMID 17079689.

          2022.02.09

          GNP_filter3_RDBDEP_html_220112.pl