Resource data sheet
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S-T7-pRc/CMV

Expressing T7-tagged fusion protein, CMV promoter.

Catalog number RDB02137
Resource name S-T7-pRc/CMV
Alternative name S-T7, single T7-tagged expression vector
Clone info. Expression vector. Expressing T7-tagged fusion protein under the control of CMV promoter.
Vector backbone pRc/CMV (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Depositor Takemoto, Yoshihiro | Hashimoto, Yasuhiro | Furuta, Masaaki | Sato, Mitsuru |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (DNA Cell Biol., 16, 893-896 (1997)).
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB02137 S-T7-pRc/CMV DNA solution Please contact us.

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB02137z.seq from Depositor
Electronic file Map RDB02137.gif from Depositor
Electronic file PDF RDB02137.pdf from Depositor

Featured content

Featured content pCMV_tag and pRSV_tag vector (English text)
Featured content Empty Backbone (English text)
Featured content Empty Backbone (Japanese text)
Featured content pCMV_tag and pRSV_tag vector (Japanese text)

References

original Takemoto, Y., Expression plasmid vectors with convenient subcloning sites in lambda gt11 that efficiently produce detectable tagged proteins. DNA Cell Biol., 16, 893-896 (1997). PMID 9260932.

User reports and related articles (go to bottom)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13052_A5A5.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB13052_A5A5a.seq
>02137_13052_A5A5_1_CMV-Forward_H01_22.ab1
    1 CTTAACTCGC CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC
  121 TCACTATAGG GAGACCCAAG CTTCCATGGC CAGCATGACC GGCGGCCAGC AGATGGGCGC
  181 GGCCGCTCGA GCATGCATCT AGAGGGCCCT ATTCTATAGT GTCACCTAAA TGCTAGAGCT
  241 CGCTGATCAG CCTCGACTGT GCCTTCTAGT TGCCAGCCAT CTGTTGTTTG CCCCTCCCCC
  301 GTGCCTTCCT TGACCCTGGA AGGTGCCACT CCCACTGTCC TTTCCTAATA AAATGAGGAA
  361 ATTGCATCGC ATTGTCTGAG TAGGTGTCAT TCTATTCTGG GGGGTGGGGT GGGGCAGGAC
  421 AGCAAGGGGG AGGATTGGGA AGACAATAGC AGGCATGCTG GGGATGCGGT GGGCTCTATG
  481 GCTTCTGAGG CGGAAAGAAC CAGCTGGGGC TCGAGGGGGG ATCCCCACGC GCCCTGTAGC
  541 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC
  601 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT
  661 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC
  721 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG
  781 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA
  841 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG
  901 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTTAAC
  961 AAAATATTAA CGCTTACAAT TTAAATATTT GCTTATACAA TCTTCCTGTT TTTGGGGCTT
 1021 TTCTGATTAT CAACCCGGGG GTGGTACCGA GCTCGAATTC TGTCAATGTG TGTTCAGTAG
 1081 GGTGTGAAAG TCCCCAGGCT CCCAGCAAGG CAGAGTATGC CAAAGCATGC ATCCTCAAAT
1141 TAGTTCAAGC CAACCCCAAA
//

Please visit Sequencing and PCR primers for primer information.

References

References

original Takemoto, Y., Expression plasmid vectors with convenient subcloning sites in lambda gt11 that efficiently produce detectable tagged proteins. DNA Cell Biol., 16, 893-896 (1997). PMID 9260932.

2018.12.11

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