Expressing T7-tagged fusion protein, CMV promoter.
| Catalog number | RDB02137 |
|---|---|
| Resource name | S-T7-pRc/CMV |
| Alternative name | S-T7, single T7-tagged expression vector |
| Clone info. | Expression vector. Expressing T7-tagged fusion protein under the control of CMV promoter. |
| Vector backbone | pRc/CMV (Plasmid) |
| Size of vector backbone | 5.5 kb |
| Selectable markers | Amp^r (E. coli), Neo^r (mammalian cell) |
| Depositor | Takemoto, Yoshihiro | Hashimoto, Yasuhiro | Furuta, Masaaki | Sato, Mitsuru | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (DNA Cell Biol., 16, 893-896 (1997)). |
|---|---|
| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB02137 | S-T7-pRc/CMV | DNA solution | Please contact us. |
Electronic file
| Electronic file | Sequence RDB02137z.seq from Depositor |
|---|---|
| Electronic file | Map RDB02137.gif from Depositor
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| Electronic file | Remarks, protocol and/or map (pdf) RDB02137.pdf from Depositor |
Original reference
Further references such as user reports and related articles (go to bottom)
Featured content
| Featured content | Empty Backbone (English text) |
|---|---|
| Featured content | Empty Backbone (Japanese text) |
| Featured content | pCMV_tag and pRSV_tag vector (English text) |
| Featured content | pCMV_tag and pRSV_tag vector (Japanese text) |
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB13052_A5A5.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: CMV-Forward | Sequence: RDB13052_A5A5a.seq |
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>02137_13052_A5A5_1_CMV-Forward_H01_22.ab1 1 CTTAACTCGC CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG 61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC 121 TCACTATAGG GAGACCCAAG CTTCCATGGC CAGCATGACC GGCGGCCAGC AGATGGGCGC 181 GGCCGCTCGA GCATGCATCT AGAGGGCCCT ATTCTATAGT GTCACCTAAA TGCTAGAGCT 241 CGCTGATCAG CCTCGACTGT GCCTTCTAGT TGCCAGCCAT CTGTTGTTTG CCCCTCCCCC 301 GTGCCTTCCT TGACCCTGGA AGGTGCCACT CCCACTGTCC TTTCCTAATA AAATGAGGAA 361 ATTGCATCGC ATTGTCTGAG TAGGTGTCAT TCTATTCTGG GGGGTGGGGT GGGGCAGGAC 421 AGCAAGGGGG AGGATTGGGA AGACAATAGC AGGCATGCTG GGGATGCGGT GGGCTCTATG 481 GCTTCTGAGG CGGAAAGAAC CAGCTGGGGC TCGAGGGGGG ATCCCCACGC GCCCTGTAGC 541 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 601 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 661 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 721 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 781 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 841 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 901 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTTAAC 961 AAAATATTAA CGCTTACAAT TTAAATATTT GCTTATACAA TCTTCCTGTT TTTGGGGCTT 1021 TTCTGATTAT CAACCCGGGG GTGGTACCGA GCTCGAATTC TGTCAATGTG TGTTCAGTAG 1081 GGTGTGAAAG TCCCCAGGCT CCCAGCAAGG CAGAGTATGC CAAAGCATGC ATCCTCAAAT 1141 TAGTTCAAGC CAACCCCAAA // |
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Sequencing and PCR primers for primer information.
Original, user report and related articles
2019.11.02
GNP_filter3_RDBDEP_html_190927.pl