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RBP-J (#RDB01801)

Mouse RBP-Jk, RBP-2N, CBF1, KBF2 cDNA

Alternative name RBP-Jk, RBP-2N, CBF1, KBF2
Clone info. Mammalian homologue of Drosophila neurogenic gene Su (H)
Vector backbone pBluescript KS (-) (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name Mouse RBP-J cDNA
Reference of insert sequence X17459 (DDBJ accession number)
Depositor|Developer Honjo, Tasuku |
 
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Terms and conditions for distribution For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Kyoto University Medical Science and Business Liaison Organization Liaison Office (contracts@kumbl.med.kyoto-u.ac.jp). Acknowledgement to Depositor in publication.When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the name of resource, RECIPIENT's name and his/her affiliation, purpose of the use, and number of resources.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01801 RBP-J DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The RBP-J was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01801).

Reference section:

Matsunami, N., Hamaguchi, Y., Yamamoto, Y., Kuze, K., Kangawa, K., Matsuo, H., Kawaichi, M., Honjo, T., A protein binding to the J kappa recombination sequence of immunoglobulin genes contains a sequence related to the integrase motif. Nature, 342, 934-937 (1989). PMID 2556644. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12401_A4Bh.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer:
Sequence file: RDB12401_A4Bha.seq check
>01801_12401_A4Bh_M13(-40)_A01_01.ab1
    1 CGGGGCCGGG GGATTTGTTA ATACGACTCA CTATAGGGCG AATTGGAGCT CCACCGCGGT
   61 GGCGGCCGCT CTAGAACTAG TGGATCCCCC GGGCTGCAGG AATTCCTGCA GGGGGGGGGG
  121 GGGGGGGGGG CCGTAGTAAG GCCCTCGGGT TTTCCCCAGC CCCCACGTAC GTCCCCAAGG
  181 GCGCGTCCCA AACCCCGGAT ACCCGGAGCC CTCCCCATGG ACTACTCGGA GGGCTTGTCC
  241 CCGAAGAAGC GGCCGGCGCA TGCTCCTTCG GCGGGAAATT TTGGGGAGCG GCCTCCCCCC
  301 AAACGACTCA TTAGGGAAGC TATGCGAAAT TATTTAAAAG AACGAGGGGA TCAAACAGGG
  361 CTCTTTCTTC TTGCAAAAGT TGCCCAAAAG TCTTACGAAA ATGAAAAACA ATTTTTTTGC
  421 CCTCCTCCTT GGGTGTATCT TAGGGGCAGG GGTGGGAAAA AAAAAAAAAA GAAAAGGGAA
  481 CGAAATGGAT GTTCTAAACA AAAGTCTCAA CCCTGGGCGT TTATTGAAAT AGGAAACAGG
  541 GACCAAAAAA TGCACCACCT GACCTTGAAA GGAAAAAACT ACTGCCCAGC CAAAACATTG
  601 TACATATCTG ATTCAAACAA AAAAAAACTT TTCTGGTTGT CTGTAAAGAT GTTCTATGGA
  661 AACAGCAATG ACATTGGTGT GTTCCTCAGC AAGCGGATAA AGGTCATCTC CAAACCCTCC
  721 AAAAAAAACC AGTCACTGAA AAATGCTGAC TTGTGCATTG CTTCAGGAAC AAAGGTGGCA
  781 CTGTTCAATC GCCTTCGGTC CCAAACAGTA AGTACCAGGT ACTGGCATGT AAAAGGAGGA
  841 AATTTCCACG CCAGTTCACA ACAGTGGGGG AGCATTTTAC ATCCTTCTCT TGAACGACAA
  901 CAAGTCGGAG GGAGAGGAGT TCACAGTTAG AGATGGCTAC TTCCATTACG GGCAACTGTC
  961 AAGCTTGGTG TGCTCATTGA CTGCCATGCA CTCCCAAGAT GATAATTAGG AAAGTTGATA
 1021 AGCAGACGGC ATTACTGGAT GCAGACCGAC CCTGTATCAC AACTCCACAA ATGTGCATTT
 1081 TACCTTAGGA TCAGAAGATG TACTTGGTGC CTTTCCTCAA GAAGGAATAT CCAATCAGCC
1141 ACTCCATGTC AAAGGAACCA AAATTAACGG CATAGTAGAT AAACGCGTA
//
Primer:
Sequence file: RDB12401_A4Bhb.seq check
>01801_12401_A4Bh_Reverse2_B01_04.ab1
    1 GTGGGTCCCA TGGATTACGG CCCAAGCCTC CGAAATTAAC CCTCACTAAA GGGAACAAAA
   61 GCTGGTACCC GGGCCCCCCC TCGCAGGTCA ACTTTTTGCT GCATTCAAGT CAGTTTCAGT
  121 CCCAGCAAAA AGACGGTAGT TAGGACACCA CGGTTGCTGT GGACGATGTG ACACTGGTAG
  181 AATTTGTGCT GGCATTTGTG TAATTCCCCT CGCTGTTTGT GTTTGACTCA TTGGAGGGCA
  241 CTTGGCTGGA GTTGGCTCTG AGAATCGCTC CTGCAGCGCT GCAGTGTGGC CTCGGCCCTG
  301 GCTCTGGTGT GTAGGTGAAG GTAAGGCTGG TGGAGTAAAT GACCCCGTCA TTTCGGACCA
  361 AAGTCACTGG AACCTGGACT GGCTGGCGGA CCCATCTCCA ACCTTCCCGG AATGCAGAAA
  421 TGTCTGGGAC CACACAGAGC ATGCTCTCTC CACATCTGTA CATTGTTTCG GCTTCTACAT
  481 CCCCAAACCA CACTCGTAAA TTTGGAGTAA AGTTTTGTCC TGTAAGTTCA AGCATTGCTA
  541 CGTCCCCGCC GCCATTCAAC TGAAGACTTT CTACGACAGG CACAGGGGTG ACTGGGGCAA
  601 GGACGGGGCC CATTCCCTCA TAGAACGTGT ATTCTGCCTT GTCTGTGCTA ATGATTGTCC
  661 AGGAAGCTCC ATCGTTTATC ATTTCCTTAT TTTGTTCTTT TGGACATGGA GTGGCCTGAA
  721 ATTGGATTAT TCTTTCTTGA GAAAGGCACA AGTACATTCT TTCTGTATCC TTAAGGTAAA
  781 ATGCACATTT GTGGAGTTGT GATACAGGGT CGTCTGCATC CAGTAATGCC GTCTGCTTAT
  841 CAAACTTTCC TAATTATCAA TCTTGGGAGT GCCATGCCCA GTCACTGAGC ACACAAAGCT
  901 TGACAGTCTG CCCGTAATGG ATGTAACCCA TCTCTAACTG TGAACTCCCT CTCCTTTCCG
  961 ACTCGTCGTC GTCCAAGAGA TGGGATGTAA AAATGCTCCC CACTGTTGTG AACTGACCGT
 1021 GGAAATTCCC CTCCTTCTAC ATGCAGGTAC CTGGTACTAA ACTGTCTGGA CCGAAAGCCG
 1081 ATGAACAGTG CCAACCTTTC GTTTCTTGAA GCAATGCAAC AGTTCAAGCC ATTCTTTCCA
 1141 GTTGACTTGC CCTTCTTTTA GAGGTGGAGA TGAACCTTTA TTCGCCTTGC TGGAGAGAAA
1201 ACACAACCCA AATGTGTTCA CTATCTCGCC TCG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Matsunami, N., A protein binding to the J kappa recombination sequence of immunoglobulin genes contains a sequence related to the integrase motif. Nature, 342, 934-937 (1989). PMID 2556644. [link to RRC of NBRP]
user_report Wang S., Osteogenic differentiation of mouse mesenchymal progenitor cell, Kusa-A1 is promoted by mammalian transcriptional repressor Rbpj. Biochem. Biophys. Res. Commun., 400, 39-45 (2010). PMID 20691157. [link to RRC of NBRP]
user_report Blanpain C., Canonical notch signaling functions as a commitment switch in the epidermal lineage. Gene. Dev., 20, 3022-3035 (2006). PMID 17079689. [link to RRC of NBRP]
user_report Kitagawa M., A human protein with sequence similarity to Drosophila mastermind coordinates the nuclear form of notch and a CSL protein to build a transcriptional activator complex on target promoters. Mol. Cell Biol., 21, 4337-4346 (2001). PMID 11390662. [link to RRC of NBRP]