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RBP-J

Mouse RBP-Jk, RBP-2N, CBF1, KBF2 cDNA

Catalog number RDB01801
Resource name RBP-J
Alternative name RBP-Jk, RBP-2N, CBF1, KBF2
Clone info. Mammalian homologue of Drosophila neurogenic gene Su (H)
Vector backbone pBluescript KS (-) (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name Mouse RBP-J cDNA
Reference of insert sequence X17459 (DDBJ accession number)
Depositor|Developer Honjo, Tasuku |
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External Database
Mouse RBP-J

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Kyoto University Medical Science and Business Liaison Organization Liaison Office (contracts@kumbl.med.kyoto-u.ac.jp). Acknowledgement to Depositor in publication.When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the name of resource, RECIPIENT's name and his/her affiliation, purpose of the use, and number of resources.
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 営利機関の利用者は、事前に京都大学・「医学領域」産学連携推進機構・知財室 (contracts@kumbl.med.kyoto-u.ac.jp) に連絡し、承諾を得ること。研究結果の発表時、謝辞に寄託者名を入れ ること。理研BRCは、本件リソースのリソース名、提供先、利用目的、件数を京都大学・本庶佑教授に報告する。
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          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB01801 RBP-J DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (gif) RDB01801.gif

          Original reference

          original Matsunami, N., A protein binding to the J kappa recombination sequence of immunoglobulin genes contains a sequence related to the integrase motif. Nature, 342, 934-937 (1989). PMID 2556644.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB12401_A4Bh.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer:
          Sequence file: RDB12401_A4Bha.seq check
          >01801_12401_A4Bh_M13(-40)_A01_01.ab1
              1 CGGGGCCGGG GGATTTGTTA ATACGACTCA CTATAGGGCG AATTGGAGCT CCACCGCGGT
             61 GGCGGCCGCT CTAGAACTAG TGGATCCCCC GGGCTGCAGG AATTCCTGCA GGGGGGGGGG
            121 GGGGGGGGGG CCGTAGTAAG GCCCTCGGGT TTTCCCCAGC CCCCACGTAC GTCCCCAAGG
            181 GCGCGTCCCA AACCCCGGAT ACCCGGAGCC CTCCCCATGG ACTACTCGGA GGGCTTGTCC
            241 CCGAAGAAGC GGCCGGCGCA TGCTCCTTCG GCGGGAAATT TTGGGGAGCG GCCTCCCCCC
            301 AAACGACTCA TTAGGGAAGC TATGCGAAAT TATTTAAAAG AACGAGGGGA TCAAACAGGG
            361 CTCTTTCTTC TTGCAAAAGT TGCCCAAAAG TCTTACGAAA ATGAAAAACA ATTTTTTTGC
            421 CCTCCTCCTT GGGTGTATCT TAGGGGCAGG GGTGGGAAAA AAAAAAAAAA GAAAAGGGAA
            481 CGAAATGGAT GTTCTAAACA AAAGTCTCAA CCCTGGGCGT TTATTGAAAT AGGAAACAGG
            541 GACCAAAAAA TGCACCACCT GACCTTGAAA GGAAAAAACT ACTGCCCAGC CAAAACATTG
            601 TACATATCTG ATTCAAACAA AAAAAAACTT TTCTGGTTGT CTGTAAAGAT GTTCTATGGA
            661 AACAGCAATG ACATTGGTGT GTTCCTCAGC AAGCGGATAA AGGTCATCTC CAAACCCTCC
            721 AAAAAAAACC AGTCACTGAA AAATGCTGAC TTGTGCATTG CTTCAGGAAC AAAGGTGGCA
            781 CTGTTCAATC GCCTTCGGTC CCAAACAGTA AGTACCAGGT ACTGGCATGT AAAAGGAGGA
            841 AATTTCCACG CCAGTTCACA ACAGTGGGGG AGCATTTTAC ATCCTTCTCT TGAACGACAA
            901 CAAGTCGGAG GGAGAGGAGT TCACAGTTAG AGATGGCTAC TTCCATTACG GGCAACTGTC
            961 AAGCTTGGTG TGCTCATTGA CTGCCATGCA CTCCCAAGAT GATAATTAGG AAAGTTGATA
           1021 AGCAGACGGC ATTACTGGAT GCAGACCGAC CCTGTATCAC AACTCCACAA ATGTGCATTT
           1081 TACCTTAGGA TCAGAAGATG TACTTGGTGC CTTTCCTCAA GAAGGAATAT CCAATCAGCC
          1141 ACTCCATGTC AAAGGAACCA AAATTAACGG CATAGTAGAT AAACGCGTA
          //
          Primer:
          Sequence file: RDB12401_A4Bhb.seq check
          >01801_12401_A4Bh_Reverse2_B01_04.ab1
              1 GTGGGTCCCA TGGATTACGG CCCAAGCCTC CGAAATTAAC CCTCACTAAA GGGAACAAAA
             61 GCTGGTACCC GGGCCCCCCC TCGCAGGTCA ACTTTTTGCT GCATTCAAGT CAGTTTCAGT
            121 CCCAGCAAAA AGACGGTAGT TAGGACACCA CGGTTGCTGT GGACGATGTG ACACTGGTAG
            181 AATTTGTGCT GGCATTTGTG TAATTCCCCT CGCTGTTTGT GTTTGACTCA TTGGAGGGCA
            241 CTTGGCTGGA GTTGGCTCTG AGAATCGCTC CTGCAGCGCT GCAGTGTGGC CTCGGCCCTG
            301 GCTCTGGTGT GTAGGTGAAG GTAAGGCTGG TGGAGTAAAT GACCCCGTCA TTTCGGACCA
            361 AAGTCACTGG AACCTGGACT GGCTGGCGGA CCCATCTCCA ACCTTCCCGG AATGCAGAAA
            421 TGTCTGGGAC CACACAGAGC ATGCTCTCTC CACATCTGTA CATTGTTTCG GCTTCTACAT
            481 CCCCAAACCA CACTCGTAAA TTTGGAGTAA AGTTTTGTCC TGTAAGTTCA AGCATTGCTA
            541 CGTCCCCGCC GCCATTCAAC TGAAGACTTT CTACGACAGG CACAGGGGTG ACTGGGGCAA
            601 GGACGGGGCC CATTCCCTCA TAGAACGTGT ATTCTGCCTT GTCTGTGCTA ATGATTGTCC
            661 AGGAAGCTCC ATCGTTTATC ATTTCCTTAT TTTGTTCTTT TGGACATGGA GTGGCCTGAA
            721 ATTGGATTAT TCTTTCTTGA GAAAGGCACA AGTACATTCT TTCTGTATCC TTAAGGTAAA
            781 ATGCACATTT GTGGAGTTGT GATACAGGGT CGTCTGCATC CAGTAATGCC GTCTGCTTAT
            841 CAAACTTTCC TAATTATCAA TCTTGGGAGT GCCATGCCCA GTCACTGAGC ACACAAAGCT
            901 TGACAGTCTG CCCGTAATGG ATGTAACCCA TCTCTAACTG TGAACTCCCT CTCCTTTCCG
            961 ACTCGTCGTC GTCCAAGAGA TGGGATGTAA AAATGCTCCC CACTGTTGTG AACTGACCGT
           1021 GGAAATTCCC CTCCTTCTAC ATGCAGGTAC CTGGTACTAA ACTGTCTGGA CCGAAAGCCG
           1081 ATGAACAGTG CCAACCTTTC GTTTCTTGAA GCAATGCAAC AGTTCAAGCC ATTCTTTCCA
           1141 GTTGACTTGC CCTTCTTTTA GAGGTGGAGA TGAACCTTTA TTCGCCTTGC TGGAGAGAAA
          1201 ACACAACCCA AATGTGTTCA CTATCTCGCC TCG
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Matsunami, N., A protein binding to the J kappa recombination sequence of immunoglobulin genes contains a sequence related to the integrase motif. Nature, 342, 934-937 (1989). PMID 2556644.
          user report Wang S., Osteogenic differentiation of mouse mesenchymal progenitor cell, Kusa-A1 is promoted by mammalian transcriptional repressor Rbpj. Biochem. Biophys. Res. Commun., 400, 39-45 (2010). PMID 20691157.
          user report Blanpain C., Canonical notch signaling functions as a commitment switch in the epidermal lineage. Gene. Dev., 20, 3022-3035 (2006). PMID 17079689.
          user report Kitagawa M., A human protein with sequence similarity to Drosophila mastermind coordinates the nuclear form of notch and a CSL protein to build a transcriptional activator complex on target promoters. Mol. Cell Biol., 21, 4337-4346 (2001). PMID 11390662.

          2022.02.09

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