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DQA10102 KAS116 3-6

DQA1*0102 cDNA, HLA- DQA1, expression, cDNA

Catalog number RDB01241
Resource name DQA10102 KAS116 3-6
Alternative name DQA1*0102 cDNA
Clone info. Expression vector of human HLA-DQA1 cDNA from EBV-B cells KAS116 library, HLA-DQA1.
This clone contains the cDNA fragments 768 bp of IMGT/HLA Acc No. HLA00603. Known difference : substitution T to C at 121nt (Phe to Leu).
Vector backbone pMAMneo (Plasmid)
Size of vector backbone 8,413 bp
Selectable markers Amp^r (E. coli)
Gene/insert name Human HLA-DQA1 cDNA
Depositor|Developer Kimura, Akinori | Senju, Satoru |
 
Other clones in our bank human HLA-DQA1 (NCBI Gene 3117) |

Distribution information

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RDB01241 DQA10102 KAS116 3-6 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content HLA Clones (en) (English text)
Featured content HLA Clones (ja) (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB01241_B1Bfp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: H2Kpro-M_F
Region: mH-2K pro,insert 5'(DQA1*01:02)
Sequence file: RDB01241_B1Bfa.seq check
>01241_01241_B1Bf_3_H2Kpro-M_F_G03_21_ABI24.ab1
    1 NNNNNNNNNN NNNGCTCTNA NCCCNNTTGG GTGGCGCGAT CACCAAGAAC CAATCAGTGT
   61 CGCCGCGGAC GCTGGATATA AAGTCCACGC AGCCCGCAGA ACTCAGAAGT CGGCTAGCAC
  121 AGCTCAGAAC AGCAACTGCT GAGGCTGCCT TGGGAAGAGG ATGATCCTAA ACAAAGCTCT
  181 GCTGCTGGGG GCCCTCGCTC TGACCACCGT GATGAGCCCC TGTGGAGGTG AAGACATTGT
  241 GGCTGACCAC GTTGCCTCTT GTGGTGTAAA CTTGTACCAG CTTTACGGTC CCTCTGGCCA
  301 GTACACCCAT GAATTTGATG GAGATGAGCA GTTCTACGTG GACCTGGAGA GGAAGGAGAC
  361 TGCCTGGCGG TGGCCTGAGT TCAGCAAATT TGGAGGTTTT GACCCGCAGG GTGCACTGAG
  421 AAACATGGCT GTGGCAAAAC ACAACTTGAA CATCATGATT AAACGCTACA ACTCTACCGC
  481 TGCTACCAAT GAGGTTCCTG AGGTCACAGT GTTTTCCAAG TCTCCCGTGA CACTGGGTCA
  541 GCCCAACACC CTCATCTGTC TTGTGGACNA CATCTTTCCT CCTGTGGTCA ACATCACATG
  601 GCTGANCAAT GGGCAGTCNG TCACAGAANG NGTTTCTNAG ANCNGCTNNN CTCCNANANT
  661 GNTCATTNCN TCNTCNNNNN NANNNNNNCN CNNCNNNNNN GNNNNNNANA NNNNNANNNN
721 NNNNNN
//
Primer: LTAlarge_upR
Region: insert 3'(DQA1*01:02)
Sequence file: RDB01241_B1Bfb.seq check
>01241_01241_B1Bf_3_LTAlarge_upR_E02_14_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNAGAATC NGTAGTTTAA CACATTATAC
   61 NCTTAAAAAT TTTATATTTA CCTTAGAGCT TTAAATCTCT GTAGGTAGTT TGTCCAATTA
  121 TGTCACACCA CAGAAGTAAG GTTCCTTCAC AAAGATCCCT CGAGTATCAC AATGGCCCTT
  181 GGTGTCTGGA AGCACCAACT GAACGCAGGC CTTGGATGAT GAAGACAGTG CCCACCACAA
  241 TGCCCATGAG GCCCACAGAC AACCCCAGGG CACAGACCAC AGTCTCTGTG AGCTCTGACA
  301 TAGGGGCTGG AATCTCAGGC TCCCAGTGTT TCAGAAGAGG CTGGTCCAGG CCCCAGTGCT
  361 CCACCTTGCA GTCATAAATC TCATCAGCAG AAGGGAGGAA GGTGAGGTAA CTGATCTTGA
  421 AGAAGGAATG ATCACTCTTG GAGAGGAAGC TGGTCTCAGA AACACCTTCT GTGACTGACT
  481 GCCCATTGCT CAGCCATGTG ATGTTGACCA CAGGAGGAAA GATGTTGTCC ACAAGACAGA
  541 TGAGGGTGTT GGGCTGANCC AGTGTCNCGG GAGACTTGGA AAACACTGTG ACCTCNNGAA
  601 CCTCATTGGT AGCNGCGGTA GANTTGTANC GNTTAATCNT GANGNTNNAG TTGTGTTTTG
  661 NCNCAGCNNN GTTTCTCANN GNNCCCTGCG NNNNAANNNT NNNANNTNNN NNANNNNNNN
721 NNN
//
Primer: SV40pro_ori_F
Region: SV40 pro_ori,f1 ori
Sequence file: RDB01241_B1Bfc.seq check
>01241_01241_B1Bf_3_SV40pro_ori_F_F02_17_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNTCNGCC TCTGAGCTAT TCNNGAAGTA GTGAGGAGGC
   61 TTTTTTGGAG GCCTANNNNN TTTGCAAAAA GCTTGCATGC CTGCAGGTCG ACTCTAGAGG
  121 ATCCCCGGGT ACCGAGCTCG AATTCACTGG CCGTCGTTTT ACAACGTCGT GACTGGGAAA
  181 ACCCTGGCGT TACCCAACTT AATCGCCTTG CAGCACATCC CCCTTTCGCC AGCTGGCGTA
  241 ATAGCGAAGA GGCCCGCACC GATCGCCCTT CCCAACAGTT GCGCAGCCTG AATGGCGAAT
  301 GGAAATTGTA AGCGTTAATA TTTTGTTAAA ATTCGCGTTA AATTTTTGTT AAATCAGCTC
  361 ATTTTTTAAC CAATAGGCCG AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA
  421 GATAGGGTTG AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC
  481 CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC CCACTACGTG AACCATCACC
  541 CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA AATCGNAACC CTNAAGGGAG
  601 CCCCCGATTT ANAGCTTGAC GGGGAAAGCC GGCGAACGTG GCGAGAAAGG AANGGAANAN
  661 NNNGAAAGGA NCGGGCGCTN GGGNGCTGGN NAGNGNANCG GNCANNCTNC NCGNNNNNNC
721 NNNNCNNNNN NNNNN
//
Primer: Amp_R
Region: Amp R pro,f1 ori
Sequence file: RDB01241_B1Bfd.seq check
>01241_01241_B1Bf_3_Amp_R_G02_20_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNGGTTAT TGTCNNCNNN GAGCGGATAC
   61 NTATTTGAAT GTATTTANAA NNATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA
  121 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC
  181 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC
  241 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG
  301 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA
  361 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC
  421 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT
  481 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA
  541 CAAAAATTTA ACGCGAATTT TAACAAAATA TTAACGCTTA CAATTTCCNT TCGCCATTCA
  601 NGNTGCGCAA CTGTNGGGAA GGGNNNNCGG NGCGGGCNNC TTCNNNNNTA CNCCANCTGG
  661 NNAANGGGGN NGTGCTGCNA NGCNANNNAN TNGGGNACGC NNNNNTTTNC CNNNNNNNAC
721 NTNNNAAACN ANNNNNNNNN N
//
Primer: lacIpro-B_F
Region: T7 pro,MMTV LTR
Sequence file: RDB01241_B1Bfe.seq check
>01241_01241_B1Bf_3_laclpro-B_F_H02_23_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNGNNN NNNTGAGCGA GGAAGNNNAN NAGCGCCCAA
   61 TACGCAAACC GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT
  121 TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TGTGAGTTAG CTCACTCATT
  181 AGGCACCCCA GGCTTTACAC TTTATGCTTC CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG
  241 GATAACAATT TCACACAGGA AACAGCTATG ACCATGATTA CGCCAAGCTC TAATACGACT
  301 CACTATAGGG AAAGCTTGGG CAGAAATGGT TGAACTCCCG AGAGTGTCCT ACACCTAGGG
  361 GAGAAGCAGC CAAGGGGTTG TTTCCCACCA AGGACGACCC GTCTGCGCAC AAACGGATGA
  421 GCCCATCAGA CAAAGACATA TTCATTCTCT GCTGCAAACT TGGCATAGCT CTGCTTTGCC
  481 TGGGGCTATT GGGGGAAGTT GCGGTTCGTG CTCGCAGGGC TCTCACCCTT GACTCTTTTA
  541 ATAGCTCTTC TGTGCAAGAT TACNATCTAA ACAATTCGGA GAACTCGACC TTCCTCCTGA
  601 NGNAAGGANC ACAGCNNACT TCCTCTTACA AGCCGCATCG ANTTTGTCCT TCANAAATAN
  661 AAATAANANN GNTTGNTAAA AANTATATTT TTANCNNTNN NNCNATNNNN NNNANANTAN
721 NANTNNNNAN NNNNNANNNA NNNTNNNNTN NNNNNNNNTT TNNNNNNNNN N
//
Primer: DQA1-H_R
Region: mH-2K pro,MMTV LTR
Sequence file: RDB01241_B1Bff.seq check
>01241_01241_B1Bf_3_DQA1-H_R_H02_23_ABI24.ab1
    1 NNNNNNNNNN NNNNNNTNNN NANTCNGANC CTGGAGTCTT CTAGCTTGGT CCCTATGGTG
   61 AGTCCGTTCC GCTCTTGTGA TGATAGCCAG ACAAGAAAGA GACAATACAA GACAAACACC
  121 AAATAGTAGA AATAGAGACA AGGGTCACTT ATCCGAGGGT CCCTGTTCGG GCGCCAGCTG
  181 CCGCAGTCGG CCGACCTGAG GGTCGCCGGG GTCTGCGGGG GGACCCTCTG GAAAGTGAAG
  241 GATAAGTGAC GAGCGGAGAC GGGATGGCGA ACAGACACAA ACACACAAGA GGTGAATGTT
  301 AGGACTGTTG CAAGTTTACT CAAAAAATCA GCACTCTTTT ATATCTTGGT TTACATAAGC
  361 ATTTACATAA GATTTGGATA AATTCCAAAA GAACATAGGA AAATAGAACA CTCAGAGCTC
  421 AGATCAGAAC CTTTGATACC AAACCAAGTC AGGAAACCAC TTGTCTCACA TCCTCGTTTT
  481 AAGAACAGTT TGTAACCAAA AACTTACTTA AGCCCTGGGA ACCGCAAGGT TGGGCCAATA
  541 AAGGCTATTC ATAATAACTC ATGCCATGAG TTTTTGCAGA ATAATGTTCT ATTAGTCCAG
  601 CCACTGTCCC CTCCTTGGTA TGGAAAATCT TTCCCCAAAA GTGCATTCCT GTTCCTAGAT
  661 AAATATAATC ATGTACCTGT TGTTTCATGT CGTCTTTTTC TTCTTGAGAC AACATACACC
  721 AAGGANGTCT AGCTCTGGCG AGTCTTTCAC GAAAAGGGAG GGATCTATAT AACACTTTAT
  781 AGCCATTGAC TGTAACCCAC CTATCCCAAT TTAAGTCATA TCTTCCTGTA TATGGTAAGG
  841 GGGCATCTGT TGGTCTGTAN ATGTAANGNC CCCTATNANT CCCTGGTTGN CACCACCTGT
  901 CTCCNATTTT GANAAAAACN NTCNTTTTTT CCCTTTTTTT ACNTCTANGN NTGNNGNCNA
  961 TANTCCTTGC ANCNGNTCTT CNNNTGNNNN TGANCGTNNN TTTNTNNTTT CNNNNCCNNN
1021 TNNNNNNNTN NNNNNNTNNN NNNAAANNNN NNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles


2021.04.21

GNP_filter3_RDBDEP_html_210401.pl