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DQA10102 KAS116 3-6

DQA1*0102 cDNA, HLA- DQA1, expression, cDNA

Catalog number RDB01241
Resource name DQA10102 KAS116 3-6
Alternative name DQA1*0102 cDNA
Clone info. Expression vector of human HLA-DQA1 cDNA from EBV-B cells KAS116 library, HLA-DQA1.
Comment This clone contains the cDNA fragments 768 bp of IMGT/HLA Acc No. HLA00603. Known difference : substitution T to C at 121nt (Phe to Leu).
Vector backbone pMAMneo (Plasmid)
Size of vector backbone 8,413 bp
Selectable markers Amp^r (E. coli)
Gene/insert name Human HLA-DQA1 cDNA
Depositor|Developer Kimura, Akinori | Senju, Satoru |
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External Database
Human HLA-DQA1

          Reference sequence
            

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          RDB01241 DQA10102 KAS116 3-6 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content HLA Clones (en) (English text)
          Featured content HLA Clones (ja) (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB01241_B1Bfp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: H2Kpro-M_F
          Region: mH-2K pro,insert 5'(DQA1*01:02)
          Sequence file: RDB01241_B1Bfa.seq check
          >01241_01241_B1Bf_3_H2Kpro-M_F_G03_21_ABI24.ab1
              1 NNNNNNNNNN NNNGCTCTNA NCCCNNTTGG GTGGCGCGAT CACCAAGAAC CAATCAGTGT
             61 CGCCGCGGAC GCTGGATATA AAGTCCACGC AGCCCGCAGA ACTCAGAAGT CGGCTAGCAC
            121 AGCTCAGAAC AGCAACTGCT GAGGCTGCCT TGGGAAGAGG ATGATCCTAA ACAAAGCTCT
            181 GCTGCTGGGG GCCCTCGCTC TGACCACCGT GATGAGCCCC TGTGGAGGTG AAGACATTGT
            241 GGCTGACCAC GTTGCCTCTT GTGGTGTAAA CTTGTACCAG CTTTACGGTC CCTCTGGCCA
            301 GTACACCCAT GAATTTGATG GAGATGAGCA GTTCTACGTG GACCTGGAGA GGAAGGAGAC
            361 TGCCTGGCGG TGGCCTGAGT TCAGCAAATT TGGAGGTTTT GACCCGCAGG GTGCACTGAG
            421 AAACATGGCT GTGGCAAAAC ACAACTTGAA CATCATGATT AAACGCTACA ACTCTACCGC
            481 TGCTACCAAT GAGGTTCCTG AGGTCACAGT GTTTTCCAAG TCTCCCGTGA CACTGGGTCA
            541 GCCCAACACC CTCATCTGTC TTGTGGACNA CATCTTTCCT CCTGTGGTCA ACATCACATG
            601 GCTGANCAAT GGGCAGTCNG TCACAGAANG NGTTTCTNAG ANCNGCTNNN CTCCNANANT
            661 GNTCATTNCN TCNTCNNNNN NANNNNNNCN CNNCNNNNNN GNNNNNNANA NNNNNANNNN
          721 NNNNNN
          //
          Primer: LTAlarge_upR
          Region: insert 3'(DQA1*01:02)
          Sequence file: RDB01241_B1Bfb.seq check
          >01241_01241_B1Bf_3_LTAlarge_upR_E02_14_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNAGAATC NGTAGTTTAA CACATTATAC
             61 NCTTAAAAAT TTTATATTTA CCTTAGAGCT TTAAATCTCT GTAGGTAGTT TGTCCAATTA
            121 TGTCACACCA CAGAAGTAAG GTTCCTTCAC AAAGATCCCT CGAGTATCAC AATGGCCCTT
            181 GGTGTCTGGA AGCACCAACT GAACGCAGGC CTTGGATGAT GAAGACAGTG CCCACCACAA
            241 TGCCCATGAG GCCCACAGAC AACCCCAGGG CACAGACCAC AGTCTCTGTG AGCTCTGACA
            301 TAGGGGCTGG AATCTCAGGC TCCCAGTGTT TCAGAAGAGG CTGGTCCAGG CCCCAGTGCT
            361 CCACCTTGCA GTCATAAATC TCATCAGCAG AAGGGAGGAA GGTGAGGTAA CTGATCTTGA
            421 AGAAGGAATG ATCACTCTTG GAGAGGAAGC TGGTCTCAGA AACACCTTCT GTGACTGACT
            481 GCCCATTGCT CAGCCATGTG ATGTTGACCA CAGGAGGAAA GATGTTGTCC ACAAGACAGA
            541 TGAGGGTGTT GGGCTGANCC AGTGTCNCGG GAGACTTGGA AAACACTGTG ACCTCNNGAA
            601 CCTCATTGGT AGCNGCGGTA GANTTGTANC GNTTAATCNT GANGNTNNAG TTGTGTTTTG
            661 NCNCAGCNNN GTTTCTCANN GNNCCCTGCG NNNNAANNNT NNNANNTNNN NNANNNNNNN
          721 NNN
          //
          Primer: SV40pro_ori_F
          Region: SV40 pro_ori,f1 ori
          Sequence file: RDB01241_B1Bfc.seq check
          >01241_01241_B1Bf_3_SV40pro_ori_F_F02_17_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNTCNGCC TCTGAGCTAT TCNNGAAGTA GTGAGGAGGC
             61 TTTTTTGGAG GCCTANNNNN TTTGCAAAAA GCTTGCATGC CTGCAGGTCG ACTCTAGAGG
            121 ATCCCCGGGT ACCGAGCTCG AATTCACTGG CCGTCGTTTT ACAACGTCGT GACTGGGAAA
            181 ACCCTGGCGT TACCCAACTT AATCGCCTTG CAGCACATCC CCCTTTCGCC AGCTGGCGTA
            241 ATAGCGAAGA GGCCCGCACC GATCGCCCTT CCCAACAGTT GCGCAGCCTG AATGGCGAAT
            301 GGAAATTGTA AGCGTTAATA TTTTGTTAAA ATTCGCGTTA AATTTTTGTT AAATCAGCTC
            361 ATTTTTTAAC CAATAGGCCG AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA
            421 GATAGGGTTG AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC
            481 CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC CCACTACGTG AACCATCACC
            541 CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA AATCGNAACC CTNAAGGGAG
            601 CCCCCGATTT ANAGCTTGAC GGGGAAAGCC GGCGAACGTG GCGAGAAAGG AANGGAANAN
            661 NNNGAAAGGA NCGGGCGCTN GGGNGCTGGN NAGNGNANCG GNCANNCTNC NCGNNNNNNC
          721 NNNNCNNNNN NNNNN
          //
          Primer: Amp_R
          Region: Amp R pro,f1 ori
          Sequence file: RDB01241_B1Bfd.seq check
          >01241_01241_B1Bf_3_Amp_R_G02_20_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNGGTTAT TGTCNNCNNN GAGCGGATAC
             61 NTATTTGAAT GTATTTANAA NNATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA
            121 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC
            181 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC
            241 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG
            301 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA
            361 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC
            421 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT
            481 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA
            541 CAAAAATTTA ACGCGAATTT TAACAAAATA TTAACGCTTA CAATTTCCNT TCGCCATTCA
            601 NGNTGCGCAA CTGTNGGGAA GGGNNNNCGG NGCGGGCNNC TTCNNNNNTA CNCCANCTGG
            661 NNAANGGGGN NGTGCTGCNA NGCNANNNAN TNGGGNACGC NNNNNTTTNC CNNNNNNNAC
          721 NTNNNAAACN ANNNNNNNNN N
          //
          Primer: lacIpro-B_F
          Region: T7 pro,MMTV LTR
          Sequence file: RDB01241_B1Bfe.seq check
          >01241_01241_B1Bf_3_laclpro-B_F_H02_23_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNGNNN NNNTGAGCGA GGAAGNNNAN NAGCGCCCAA
             61 TACGCAAACC GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT
            121 TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TGTGAGTTAG CTCACTCATT
            181 AGGCACCCCA GGCTTTACAC TTTATGCTTC CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG
            241 GATAACAATT TCACACAGGA AACAGCTATG ACCATGATTA CGCCAAGCTC TAATACGACT
            301 CACTATAGGG AAAGCTTGGG CAGAAATGGT TGAACTCCCG AGAGTGTCCT ACACCTAGGG
            361 GAGAAGCAGC CAAGGGGTTG TTTCCCACCA AGGACGACCC GTCTGCGCAC AAACGGATGA
            421 GCCCATCAGA CAAAGACATA TTCATTCTCT GCTGCAAACT TGGCATAGCT CTGCTTTGCC
            481 TGGGGCTATT GGGGGAAGTT GCGGTTCGTG CTCGCAGGGC TCTCACCCTT GACTCTTTTA
            541 ATAGCTCTTC TGTGCAAGAT TACNATCTAA ACAATTCGGA GAACTCGACC TTCCTCCTGA
            601 NGNAAGGANC ACAGCNNACT TCCTCTTACA AGCCGCATCG ANTTTGTCCT TCANAAATAN
            661 AAATAANANN GNTTGNTAAA AANTATATTT TTANCNNTNN NNCNATNNNN NNNANANTAN
          721 NANTNNNNAN NNNNNANNNA NNNTNNNNTN NNNNNNNNTT TNNNNNNNNN N
          //
          Primer: DQA1-H_R
          Region: mH-2K pro,MMTV LTR
          Sequence file: RDB01241_B1Bff.seq check
          >01241_01241_B1Bf_3_DQA1-H_R_H02_23_ABI24.ab1
              1 NNNNNNNNNN NNNNNNTNNN NANTCNGANC CTGGAGTCTT CTAGCTTGGT CCCTATGGTG
             61 AGTCCGTTCC GCTCTTGTGA TGATAGCCAG ACAAGAAAGA GACAATACAA GACAAACACC
            121 AAATAGTAGA AATAGAGACA AGGGTCACTT ATCCGAGGGT CCCTGTTCGG GCGCCAGCTG
            181 CCGCAGTCGG CCGACCTGAG GGTCGCCGGG GTCTGCGGGG GGACCCTCTG GAAAGTGAAG
            241 GATAAGTGAC GAGCGGAGAC GGGATGGCGA ACAGACACAA ACACACAAGA GGTGAATGTT
            301 AGGACTGTTG CAAGTTTACT CAAAAAATCA GCACTCTTTT ATATCTTGGT TTACATAAGC
            361 ATTTACATAA GATTTGGATA AATTCCAAAA GAACATAGGA AAATAGAACA CTCAGAGCTC
            421 AGATCAGAAC CTTTGATACC AAACCAAGTC AGGAAACCAC TTGTCTCACA TCCTCGTTTT
            481 AAGAACAGTT TGTAACCAAA AACTTACTTA AGCCCTGGGA ACCGCAAGGT TGGGCCAATA
            541 AAGGCTATTC ATAATAACTC ATGCCATGAG TTTTTGCAGA ATAATGTTCT ATTAGTCCAG
            601 CCACTGTCCC CTCCTTGGTA TGGAAAATCT TTCCCCAAAA GTGCATTCCT GTTCCTAGAT
            661 AAATATAATC ATGTACCTGT TGTTTCATGT CGTCTTTTTC TTCTTGAGAC AACATACACC
            721 AAGGANGTCT AGCTCTGGCG AGTCTTTCAC GAAAAGGGAG GGATCTATAT AACACTTTAT
            781 AGCCATTGAC TGTAACCCAC CTATCCCAAT TTAAGTCATA TCTTCCTGTA TATGGTAAGG
            841 GGGCATCTGT TGGTCTGTAN ATGTAANGNC CCCTATNANT CCCTGGTTGN CACCACCTGT
            901 CTCCNATTTT GANAAAAACN NTCNTTTTTT CCCTTTTTTT ACNTCTANGN NTGNNGNCNA
            961 TANTCCTTGC ANCNGNTCTT CNNNTGNNNN TGANCGTNNN TTTNTNNTTT CNNNNCCNNN
          1021 TNNNNNNNTN NNNNNNTNNN NNNAAANNNN NNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles


          2022.05.18

          GNP_filter3_RDBDEP_html_220516.pl