DQA1*0501 cDNA, HLA- DQA1, expression, cDNA
| Catalog number | RDB01239 |
|---|---|
| Resource name | DQA10501 AMALA-4 |
| Alternative name | DQA1*0501 cDNA |
| Clone info. | Expression vector of human HLA-DQA1 cDNA from EBV-B cells AMALA library, HLA-DQA1 |
| Vector backbone | pMAMneo (Plasmid) |
| Size of vector backbone | 8,413 bp |
| Selectable markers | Amp^r (E. coli), Neo^r (cell) |
| Gene/insert name | Human HLA-DQA1 cDNA |
| Depositor | Kimura, Akinori | Senju, Satoru | |
| Other clones in our bank | human HLA-DQA1 (NCBI Gene 3117) | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | This material is provided with written consent of Depositor. |
|---|---|
| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Approval form by depositor [Word] |
| Remarks | ((Additional form)) Approval Form(FormD) Please obtain written approval of the depositor. |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB01239 | DQA10501 AMALA-4 | DNA solution | Please contact us. |
Electronic file
| Electronic file | Map RDB01239.gif from Depositor
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Featured content
| Featured content | HLA Clones (en) (English text) |
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| Featured content | HLA Clones (ja) (Japanese text) |
References
User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB15307_A7Gsp1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: pMAMneo-Forward | RDB15307_A7Gsa.seq |
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>1239_15307_A7Gs_pMAMneo-Forward_D02_11_ABI24.ab1 1 TTAAGGAGCG GACGGACTCA CCATAGGGAC CAAGCTAGAA GACTCCAGGG TCTGACTTCT 61 GAAGAGAAGA AGAAAGAGGA AGAGTGGAAG AGAGGACACA GAGAGTCTGG CCTGCGGGTC 121 TCTCCTGGTG TTTTGAGAGT TTCTGGATCA GAACTCGGAG ACGACAGCAC AGGGTTCAGG 181 CAAAGTCTTA GTCGCCAGGC AGTGAGGTCA GGGGTGGGGA AGCCCAGGGC TGGGGATTCC 241 CCATCTCCAC AGTTTCACTT CTGCACCTAA CCTGGGTCAG GTCCTTCTGT CCGGACACTG 301 TTGACGCGCA GTCAGCTCTT ACCCCCATTG GGTGGCGCGA TCACCAAGAA CCAATCAGTG 361 TCGCCGCGGA CGCTGGATAT AAAGTCCACG CAGCCCGCAG AACTCAGAAG TCGGCTAGCA 421 CAGCTCAGAA CAGCAACTGC TAAGGCTGCC TTGGGAAGAG GATGATCCTA AACAAAGCTC 481 TGATGCTGGG GGCCCTTGCC CTGACCACCG TGATGAGCCC CTGTGGAGGT GAAGACATTG 541 TGGCTGACCA CGTCGCCTCT TATGGTGTAA ACTTGTACCA GTCTTACGGT CCCTCTGGCC 601 AGTACACCCA TGAATTTGAT GGAGATGAGC AGTTCTACGT GGACCTGGGG AGGAAGGAGA 661 CTGTCTGGTG TTTGCCTGTT CTCAGACAAT TTAGATTTGA CCCGCAATTT GCACTGACAA 721 ACATCGCTGT CCTAAAACAT AACTTGAACA GTCTGATTAA ACGCTCCAAC TCTACCGCTG 781 CTACCAATGA GGTTCCTGAG GTCACAGTGT TTTCCAAGTC TCCCGTGACA CTGGGTCAGC 841 CCAACATCCT CATCTGTCTT GTGGACAACA TCTTTCCTCC TGTGGTCAAC ATCACATGGC 901 TGAGCAATGG GCACTCAGTC ACAGAAGGTG TTTCTGAGAC CAGCTTCCTC TCCAAGAGTG 961 ATCATTCCTT CTTCAAGATC AGTTACCTCA CCCTCCTTCC CTTTCTTTCT GAGGAGAGTT 1021 ATGACTGCAA AGTGGAGCAC TGGGGGCCCT GCACAAGCCT CCTTCCTGAA ACACTGGGGA 1081 GCCCTGAGAT CAAGCCCCTA TGGTCAGAGC TCACAGAAGA ACTTGGTTGG GATTCTTG // |
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| Primer: RDB01239#1 | RDB15307_A7Gsb.seq |
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>01239_15307_A7Gs_RDB01239#1_G04_19_ABI24.ab1 1 ACAACCTCAA AGTGATCATT CCTTCTTCAG ATCAGTTACC TCACCCTCCT CCCTTCTTCT 61 GAGGAGAGTT ATGACTGCAA GGTGGAGCAC TGGGGCCTGG ACAAGCCTCT TCTGAAACAC 121 TGGGAGCCTG AGATTCCAGC CCCTATGTCA GAGCTCACAG AGACTGTGGT CTGCGCCCTG 181 GGATTGTCTG TGGGCCTCGT GGGCATTGTG GTGGGCACTG TCTTCATCAT CCGAGGCCTG 241 CGTTCAGTTG GTGCTTCCAG ACACCAAGGG CCCTTGTGAT ACTCGAGGGA TCTTTGTGAA 301 GGAACCTTAC TTCTGTGGTG TGACATAATT GGACAAACTA CCTACAGAGA TTTAAAGCTC 361 TAAGGTAAAT ATAAAATTTT TAAGTGTATA ATGTGTTAAA CTACTGATTC TAATTGTTTG 421 TGTATTTTAG ATTCCAACCT ATGGAACTGA TGAATGGGAG CAGTGGTGGA ATGCCTTTAA 481 TGAGGAAAAC CTGTTTTGCT CAGAAGAAAT GCCATCTAGT GATGATGAGG CTACTGCTGA 541 CTCTCAACAT TCTACTCCTC CAAAAAAGAA GAGAAAGGTA GAAGACCCCA AGGACTTTCC 601 TTCAGAATTG CTAAGTTTTT TGAGTCATGC TGTGTTTAGT AATAGAACTC TTGCTTGCTT 661 TGCTATTTAC ACCACAAAGG AAAAAGCTGC ACTGCTATAC AAGAAAATTA TGGAAAAATA 721 TTCTGTAACC TTTATAAGTA GGCATAACAG TTATAATCAT AACATACTGT TTTTTCTTAC 781 TCCACACAGG CATAGAGTGT CTGCTATTAA TAACTATGCT CAAAAATTGT GTACCTTTAG 841 CTTTTTAATT TGTAAAGGGG GTTAATAAGG AATATTTGAT GTATAGTGCC TTGACTAGAG 901 ATCATAATCA GCCATACCAC ATTTGTAGAG GTTTTACTTG CTTTAAAAAA CCTTCCCACA 961 CCCTCCCCCC TGAACCTGAA ACATAAAATG AATGCAATTG TTGTTGTTAA CTTGTTTATT 1021 TGCAGCTTAT ATGGTTACAA TAAAGCAATT AGCATCACAA ATTTCACAAA TTAAGCATTT 1081 TTTTCACTGC CAATTCTAGT GATGGTTTGT TTCCGAC // |
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| Primer: MMTV-LTR#1 | RDB15307_A7Gsc.seq |
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>01239_15307_A7Gs_MMTV-LTR#1_E02_14_ABI24.ab1 1 GGAACCCAGG GGCAAAGCAG GAGCTATGCC AAGTTTGCAG CAGAGAATGA ATATGTCTTT 61 GTCTGATGGG CTCATCCGTT TGTGCGCAGA CGGGTCGTCC TTGGTGGGAA ACAACCCCTT 121 GGCTGCTTCT CCCCTAGGTG TAGGACACTC TCGGGAGTTC AACCATTTCT GCCCAAGCTT 181 TCCCTATAGT GAGTCGTATT AGAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG 241 AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC 301 CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT 361 CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG 421 CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT 481 TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 541 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 601 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 661 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 721 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 781 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 841 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCC GTTCAGCCCG 901 ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT 961 CGCCACTGGC AGCAGCCACT GGGTAACAGG ATTAGCAGAG CGAGGGTATG TAGGCGGTGC 1021 TACAGAGTTT CTTTGAAGTG GTGGCCTAAC TACCGGCTAC ACTAGCAAGA ACAAGGTATT 1081 TGGTATCCTG CGCCTCTGCC TGAAGGCCAA GGTTAACC // |
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Sequencing and PCR primers for primer information.
References
2018.12.10
GNP_filter3_RDBDEP_html_181012.pl