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DQA10501 AMALA-4

DQA1*0501 cDNA, HLA- DQA1, expression, cDNA

Catalog number RDB01239
Resource name DQA10501 AMALA-4
Alternative name DQA1*0501 cDNA
Clone info. Expression vector of human HLA-DQA1 cDNA from EBV-B cells AMALA library, HLA-DQA1
Vector backbone pMAMneo (Plasmid)
Size of vector backbone 8,413 bp
Selectable markers Amp^r (E. coli), Neo^r (cell)
Gene/insert name Human HLA-DQA1 cDNA
Depositor Kimura, Akinori | Senju, Satoru |
 
Other clones in our bank human HLA-DQA1 (NCBI Gene 3117) |

Distribution information

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RDB01239 DQA10501 AMALA-4 DNA solution Please contact us.

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Map RDB01239.gif from Depositor

Featured content

Featured content HLA Clones (en) (English text)
Featured content HLA Clones (ja) (Japanese text)

References

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15307_A7Gsp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pMAMneo-Forward RDB15307_A7Gsa.seq
>1239_15307_A7Gs_pMAMneo-Forward_D02_11_ABI24.ab1
    1 TTAAGGAGCG GACGGACTCA CCATAGGGAC CAAGCTAGAA GACTCCAGGG TCTGACTTCT
   61 GAAGAGAAGA AGAAAGAGGA AGAGTGGAAG AGAGGACACA GAGAGTCTGG CCTGCGGGTC
  121 TCTCCTGGTG TTTTGAGAGT TTCTGGATCA GAACTCGGAG ACGACAGCAC AGGGTTCAGG
  181 CAAAGTCTTA GTCGCCAGGC AGTGAGGTCA GGGGTGGGGA AGCCCAGGGC TGGGGATTCC
  241 CCATCTCCAC AGTTTCACTT CTGCACCTAA CCTGGGTCAG GTCCTTCTGT CCGGACACTG
  301 TTGACGCGCA GTCAGCTCTT ACCCCCATTG GGTGGCGCGA TCACCAAGAA CCAATCAGTG
  361 TCGCCGCGGA CGCTGGATAT AAAGTCCACG CAGCCCGCAG AACTCAGAAG TCGGCTAGCA
  421 CAGCTCAGAA CAGCAACTGC TAAGGCTGCC TTGGGAAGAG GATGATCCTA AACAAAGCTC
  481 TGATGCTGGG GGCCCTTGCC CTGACCACCG TGATGAGCCC CTGTGGAGGT GAAGACATTG
  541 TGGCTGACCA CGTCGCCTCT TATGGTGTAA ACTTGTACCA GTCTTACGGT CCCTCTGGCC
  601 AGTACACCCA TGAATTTGAT GGAGATGAGC AGTTCTACGT GGACCTGGGG AGGAAGGAGA
  661 CTGTCTGGTG TTTGCCTGTT CTCAGACAAT TTAGATTTGA CCCGCAATTT GCACTGACAA
  721 ACATCGCTGT CCTAAAACAT AACTTGAACA GTCTGATTAA ACGCTCCAAC TCTACCGCTG
  781 CTACCAATGA GGTTCCTGAG GTCACAGTGT TTTCCAAGTC TCCCGTGACA CTGGGTCAGC
  841 CCAACATCCT CATCTGTCTT GTGGACAACA TCTTTCCTCC TGTGGTCAAC ATCACATGGC
  901 TGAGCAATGG GCACTCAGTC ACAGAAGGTG TTTCTGAGAC CAGCTTCCTC TCCAAGAGTG
  961 ATCATTCCTT CTTCAAGATC AGTTACCTCA CCCTCCTTCC CTTTCTTTCT GAGGAGAGTT
 1021 ATGACTGCAA AGTGGAGCAC TGGGGGCCCT GCACAAGCCT CCTTCCTGAA ACACTGGGGA
1081 GCCCTGAGAT CAAGCCCCTA TGGTCAGAGC TCACAGAAGA ACTTGGTTGG GATTCTTG
//
Primer: RDB01239#1 RDB15307_A7Gsb.seq
>01239_15307_A7Gs_RDB01239#1_G04_19_ABI24.ab1
    1 ACAACCTCAA AGTGATCATT CCTTCTTCAG ATCAGTTACC TCACCCTCCT CCCTTCTTCT
   61 GAGGAGAGTT ATGACTGCAA GGTGGAGCAC TGGGGCCTGG ACAAGCCTCT TCTGAAACAC
  121 TGGGAGCCTG AGATTCCAGC CCCTATGTCA GAGCTCACAG AGACTGTGGT CTGCGCCCTG
  181 GGATTGTCTG TGGGCCTCGT GGGCATTGTG GTGGGCACTG TCTTCATCAT CCGAGGCCTG
  241 CGTTCAGTTG GTGCTTCCAG ACACCAAGGG CCCTTGTGAT ACTCGAGGGA TCTTTGTGAA
  301 GGAACCTTAC TTCTGTGGTG TGACATAATT GGACAAACTA CCTACAGAGA TTTAAAGCTC
  361 TAAGGTAAAT ATAAAATTTT TAAGTGTATA ATGTGTTAAA CTACTGATTC TAATTGTTTG
  421 TGTATTTTAG ATTCCAACCT ATGGAACTGA TGAATGGGAG CAGTGGTGGA ATGCCTTTAA
  481 TGAGGAAAAC CTGTTTTGCT CAGAAGAAAT GCCATCTAGT GATGATGAGG CTACTGCTGA
  541 CTCTCAACAT TCTACTCCTC CAAAAAAGAA GAGAAAGGTA GAAGACCCCA AGGACTTTCC
  601 TTCAGAATTG CTAAGTTTTT TGAGTCATGC TGTGTTTAGT AATAGAACTC TTGCTTGCTT
  661 TGCTATTTAC ACCACAAAGG AAAAAGCTGC ACTGCTATAC AAGAAAATTA TGGAAAAATA
  721 TTCTGTAACC TTTATAAGTA GGCATAACAG TTATAATCAT AACATACTGT TTTTTCTTAC
  781 TCCACACAGG CATAGAGTGT CTGCTATTAA TAACTATGCT CAAAAATTGT GTACCTTTAG
  841 CTTTTTAATT TGTAAAGGGG GTTAATAAGG AATATTTGAT GTATAGTGCC TTGACTAGAG
  901 ATCATAATCA GCCATACCAC ATTTGTAGAG GTTTTACTTG CTTTAAAAAA CCTTCCCACA
  961 CCCTCCCCCC TGAACCTGAA ACATAAAATG AATGCAATTG TTGTTGTTAA CTTGTTTATT
 1021 TGCAGCTTAT ATGGTTACAA TAAAGCAATT AGCATCACAA ATTTCACAAA TTAAGCATTT
1081 TTTTCACTGC CAATTCTAGT GATGGTTTGT TTCCGAC
//
Primer: MMTV-LTR#1 RDB15307_A7Gsc.seq
>01239_15307_A7Gs_MMTV-LTR#1_E02_14_ABI24.ab1
    1 GGAACCCAGG GGCAAAGCAG GAGCTATGCC AAGTTTGCAG CAGAGAATGA ATATGTCTTT
   61 GTCTGATGGG CTCATCCGTT TGTGCGCAGA CGGGTCGTCC TTGGTGGGAA ACAACCCCTT
  121 GGCTGCTTCT CCCCTAGGTG TAGGACACTC TCGGGAGTTC AACCATTTCT GCCCAAGCTT
  181 TCCCTATAGT GAGTCGTATT AGAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG
  241 AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC
  301 CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT
  361 CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG
  421 CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT
  481 TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC
  541 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA
  601 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA
  661 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC
  721 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC
  781 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG
  841 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCC GTTCAGCCCG
  901 ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT
  961 CGCCACTGGC AGCAGCCACT GGGTAACAGG ATTAGCAGAG CGAGGGTATG TAGGCGGTGC
 1021 TACAGAGTTT CTTTGAAGTG GTGGCCTAAC TACCGGCTAC ACTAGCAAGA ACAAGGTATT
1081 TGGTATCCTG CGCCTCTGCC TGAAGGCCAA GGTTAACC
//

Please visit Sequencing and PCR primers for primer information.


References

References


2018.12.10

GNP_filter3_RDBDEP_html_181012.pl