Resource data sheet
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K^b DRB1 1201

DRB1*1201 cDNA

Catalog number RDB01236
Resource name K^b DRB1 1201
Alternative name DRB1*1201 cDNA
Clone info. Human HLA-DRB1 cDNA from EBV-B cells BM16 library, HLA-DRB1, H-2K^b promoter
Vector backbone pMAMneo (Plasmid)
Size of vector backbone 8,413 bp
Selectable markers Amp^r (E. coli), Neo^r (cell)
Gene/insert name Human HLA-DRB1 cDNA
Depositor Kimura, Akinori | Senju, Satoru |
 
Other clones in our bank human HLA-DPB1 (NCBI Gene 3115) |

Distribution information

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RDB01236 K^b DRB1 1201 DNA solution Please contact us.

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Map RDB01236.gif from Depositor

Featured content

Featured content HLA Clones (en) (English text)
Featured content HLA Clones (ja) (Japanese text)

References

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15306_A7Gsp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pMAMneo-Forward RDB15306_A7Gsa.seq
>1236_15306_A7Gs_pMAMneo-Forward_A02_02_ABI24.ab1
    1 TAAAGGGAGC GGACGGACTC ACCATAGGGA CCAAGCTAGA AGACTCCAGG GTCTGACTTC
   61 TGAAGAGAAG AAGAAAGAGG AAGAGTGGAA GAGAGGACAC AGAGAGTCTG GCCTGCGGGT
  121 CTCTCCTGGT GTTTTGAGAG TTTCTGGATC AGAACTCGGA GACGACAGCA CAGGGTTCAG
  181 GCAAAGTCTT AGTCGCCAGG CAGTGAGGTC AGGGGTGGGG AAGCCCAGGG CTGGGGATTC
  241 CCCATCTCCA CAGTTTCACT TCTGCACCTA ACCTGGGTCA GGTCCTTCTG TCCGGACACT
  301 GTTGACGCGC AGTCAGCTCT TACCCCCATT GGGTGGCGCG ATCACCAAGA ACCAATCAGT
  361 GTCGCCGCGG ACGCTGGATA TAAAGTCCAC GCAGCCCGCA GAACTCAGAA GTCGGCTAGC
  421 TGGCCCCTGG TCCTGTCCTG TTCTCCAGCA TGGTGTGTCT GAGGCTCCCT GGAGGCTCCT
  481 GCATGGCAGT TCTGACAGTG ACACTGATGG TGCTGAGCTC CCCACTGGCT TTGGCTGGGG
  541 ACACCAGACC ACGTTTCTTG GAGTACTCTA CGGGTGAGTG TTATTTCTTC AATGGGACGG
  601 AGCGGGTGCG GTTACTGGGG AGACACTTCC ATAACCAGGA GGAGCTCCTG CGCTTCGACA
  661 GCGACGTGGG GGAGTTCCGG GCGGTGACGG AGCTGGGGCG GCCTGTCGCC GAGTCCTGGA
  721 ACAGCCAGAA GGACATCCTG GAAGACAGGC GCGCCGCGGT GGACACCTAT TGCAGACACA
  781 ACTACGGGGC TGTGGAGAGC TTCACAGTGC AGCGGCGAGT CCATCCTAAG GTGACTGTGT
  841 ATCCTTCAAA GACCCAGCCC CTGCAGCACC ACAACCTCCT GGTCTGTTCT GTGAGTGGTT
  901 TCTATCCAGG CAGCATTGAA GTCAGGTGGT TCCGGAATGG CCAGGAAGAG AAGACTGGGG
  961 GTGGTGTCCA CGGGCCCTGA TCCACAATGG TAGACTGGAA CCTTCCAGAC CCTGCTGATG
 1021 CTGGAAAACA AGTTTCCTCG GGAGTGGAAG AAGGTTTAAC ACCTGCCCAA AGTGAAGCAC
 1081 CCAAGCGTGA ACAAGCCTTC CTCACAGTGA ATGGAGAGCA CGGATCTGAA TTCTTGCCAC
1141 CAGGAAGCCG ATG
//
Primer: RDB01236#1 RDB15306_A7Gsb.seq
>01236_15306_A7Gs_RDB01236#1_E04_13_ABI24.ab1
    1 CAAGGCCGCA TTGAGTCAGG TGGTTCCGGA TGGCCAGGAA GAGAAGACTG GGGTGGTGTC
   61 CACGGGCCTG ATCCACAATG GAGACTGGAC CTTCCAGACC CTGGTGATGC TGGAAACAGT
  121 TCCTCGGAGT GGAGAGGTTT ACACCTGCCA AGTGGAGCAC CCAAGCGTGA CAAGCCCTCT
  181 CACAGTGGAA TGGAGAGCAC GGTCTGAATC TGCACAGAGC AAGATGCTGA GTGGAGTCGG
  241 GGGCTTTGTG CTGGGCCTGC TCTTCCTTGG GGCCGGGCTG TTCATCTACT TCAGGAATCA
  301 GAAAGGACAC TCTGGACTTC AGCCAAGAGG ATTCCTGAGC TGAAATGCGG ATGACCTCGA
  361 GGGATCTTTG TGAAGGAACC TTACTTCTGT GGTGTGACAT AATTGGACAA ACTACCTACA
  421 GAGATTTAAA GCTCTAAGGT AAATATAAAA TTTTTAAGTG TATAATGTGT TAAACTACTG
  481 ATTCTAATTG TTTGTGTATT TTAGATTCCA ACCTATGGAA CTGATGAATG GGAGCAGTGG
  541 TGGAATGCCT TTAATGAGGA AAACCTGTTT TGCTCAGAAG AAATGCCATC TAGTGATGAT
  601 GAGGCTACTG CTGACTCTCA ACATTCTACT CCTCCAAAAA AGAAGAGAAA GGTAGAAGAC
  661 CCCAAGGACT TTCCTTCAGA ATTGCTAAGT TTTTTGAGTC ATGCTGTGTT TAGTAATAGA
  721 ACTCTTGCTT GCTTTGCTAT TTACACCACA AAGGAAAAAA GCTGCACTGC TATACAAGAA
  781 AATTATGGAA AAATATTCTG TAACCTTTAT AAGTAGGCAT AACAGTTATA ATCATAACAT
  841 ACTGTTTTTT TCTTACTCCA CACAGGCATA GAGTGTCTGC TATTAATAAC TATGCTCAAA
  901 AATTGTGTAC CTTTAGCTTT TTTAATTTGT AAAGGGGTTA ATAAAGGAAT ATTTGATGTA
  961 TAGTGCCCTT GACTAGAGAT CATAATCAGC CCATACCCAC ATTTGTAGAG GTTTTACTTT
 1021 GCTTTAAAAA AACCTCCAAC ACTCCCCCCT GAATCTGAAA ACATAAAATG GAAATGGCAA
1081 GTGATGGGTG TTACTTGCTA TGGCAGCCTA ATAATTGCTT AACGAATAAA AAGGTCA
//
Primer: MMTV-LTR#1 RDB15306_A7Gsc.seq
>01236_15306_A7Gs_MMTV-LTR#1_D02_11_ABI24.ab1
    1 AAGCCCAGGG GCAAGCAGAG CTATGCCAAG TTTGCAGCAG AGAATGAATA TGTCTTTGTC
   61 TGATGGGCTC ATCCGTTTGT GCGCAGACGG GTCGTCCTTG GTGGGAAACA ACCCCTTGGC
  121 TGCTTCTCCC CTAGGTGTAG GACACTCTCG GGAGTTCAAC CATTTCTGCC CAAGCTTTCC
  181 CTATAGTGAG TCGTATTAGA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA
  241 TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG
  301 GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA
  361 GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG
  421 TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG
  481 GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG
  541 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA
  601 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG
  661 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC
  721 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC
  781 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC
  841 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCCGTT TCAGCCCGAC
  901 CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG
  961 CCACTGGCAG CAGCCCACTG ATAACAGGAT TAGCAGAGCG AGGGTATGTA GGCGGTGCTA
 1021 CAGAGTTCTT TGAAAGTGGT GGGCCCTACC TACCGCCTAC ACTAGCAGAC CAGTATTGGT
1081 ATTCTGCGCC TCTGCCTGGA AGCCAAGTAT TAACCGA
//

Please visit Sequencing and PCR primers for primer information.


References

References


2018.12.10

GNP_filter3_RDBDEP_html_181012.pl