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pg DQ1BS

DQw6beta short, Short cytoplasmic from DQB, DQB1*0601, HLA-DQB1 (genomic DNA+cDNA)

Catalog number RDB01230
Resource name pg DQ1BS
Alternative name DQw6 beta short
Clone info. Human HLA-DQB1 genomic DNA and cDNA from EBV-B cells TOK library, Short cytoplasmic from DQB, DQB1*0601, HLA-DQB1
Vector backbone pTZ19R (Plasmid)
Size of vector backbone 2.9 kb
Selectable markers Amp^r
Gene/insert name Human HLA-DQB1 Genomic DNA + cDNA
Depositor|Developer Kimura, Akinori | Senju, Satoru |
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External Database
Human HLA-DQB1

          Reference sequence
            

          Distribution information

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          Terms and conditions set forth by the DEPOSITOR For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
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          RDB01230 pg DQ1BS DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Senju, S., Allele-specific expression of the cytoplasmic exon of HLA-DQB1 gene. Immunogenetics, 36, 319-325 (1992). PMID 1644449.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content HLA Clones (en) (English text)
          Featured content HLA Clones (ja) (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB01230_B1Bfp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pUC-CAPbs_up_F2
          Region: T7 pro,insert 5'(b region,c region 5')
          Sequence file: RDB01230_B1Bfa.seq check
          >01230_01230_B1Bf_1_pUC-CAPbs_up_F2_H02_23_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNGC TTNNNNCTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT
             61 GTGAGCGGAT AACAATTTCA CACAGGAAAC AGCTATGACC ATGATTACGC CAAGCTCTAA
            121 TACGACTCAC TATAGGGAAA GCTTCAGGCT TGGCCTGGTG GAGTTAGGGC TGCCCCACAA
            181 CTGCGCTCAG GGCATCCAGC AATTACAGTT GCGAAATAAG ATATTTTGAC TTTTTGGCTT
            241 CAAATCATTA TTCATCGTAA TTCTGTTTTC TTAAATGGCT ATCACTCATG GCGGAGATCT
            301 TTGAGGCGAG AGACTGTTTA ATTATTGCAT GCTTGGTACC ATTTCTTCTG AAGCTATTCC
            361 AAGTAATTGA AAAGGAGGAC TCCTCTCTAA CTCATTTTAT GAGGCCAGCG TCATCCTGAT
            421 ACCAAAGCCT GACAGAGATA CAACAAAAAA GGAAAACTTC AGGCCAATAT CACTGATGGA
            481 CACTGATTAA AAAATCCTCA ATAAAATACT GGCAAGCCGA ATCCACCAGC ACATCAAAAA
            541 GTTCATCCAC CACGATCAAG TTGGCATCAT CCCCAGGANG CCNGGCTGTT TCNACATATG
            601 CNAATCNATA NACATAATTC ATTACATAAA CNGAATTNAA GANAAAAACN ACATGATTAN
            661 CTNNANAGAC NCNGAAAANN NNNNCNNTAA AANTNANNNT CCNTTNNNGN NNAANNNCNC
          721 NNNANNNNNN NNNN
          //
          Primer: pGL3basic_4406F
          Region: f1 ori,M13 fwd,insert 3'(e region 3')
          Sequence file: RDB01230_B1Bfb.seq check
          >01230_01230_B1Bf_1_pGL3basic_4406F_A01_01_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNGNNNNNN NNNAGCTGAT TTAACAAAAA TTTAACGCGA
             61 ATTTTAACAA AATNNNNNNC GCTTACAATT TCCATTCGCC ATTCAGGCTG CGCAACTGTT
            121 GGGAAGGGCG ATCGGTGCGG GCCTCTTCGC TATTACGCCA GCTGGCGAAA GGGGGATGTG
            181 CTGCAAGGCG ATTAAGTTGG GTAACGCCAG GGTTTTCCCA GTCACGACGT TGTAAAACGA
            241 CGGCCAGTGA ATTCGAGCTC TGCCATTTGT CCTGTGGGCT CCACAAGAAA CAAACTGCCC
            301 CTTACACCCC TCCACTGTGA GGAGGCTGTG GAGGCTGAGG TGCTCCACAT GGCTGGTGTA
            361 GACATCTGCA CACTGGAAGT CATTTCCAGG ACCACAAGGA TCTGGAAAAT CCAGTCCTCC
            421 TTCCTAATAA GAGCGATGGA CACAATGCTG GCTGTCAGCA TCCCGTGCAC AGGACAATGT
            481 GTTTGAGAGG TGTGCATGTT ACCCAGGCTT GGCCAATCAG AATCTTTCCT AAAGTTGTTA
            541 AAACTCTGGT AGACATTCTA GAAACATATA TTAACAGAGA CAGACAAACA GACACACACA
            601 CACACACACA CACACACACA CACACAGCAA GAGAGAGAGA TGAGATAAGA NATGAAGTGA
            661 TAANGNAGAN AAATGCAGAA AAATAGATGC AAAAAGAAAC ACGGNNNTAG AAAAAANNGN
          721 ANANAANCNN NNNNNNANNN GNNNNNNAN
          //
          Primer: 0601-exon2_F2
          Region: insert mid(c region-exon 2 3')
          Sequence file: RDB01230_B1Bfc.seq check
          >01230_01230_1_B1Bf_0601-exon2_F2_D03_12_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN CNTCTATAAC CGNNNNNNNG NNGTGCGCTT
             61 CGACAGCGAC GTGGGGGTGT ATCGGGCGGT GACGCCGCAG GGGCGGCCTG ACGCCGAGTA
            121 CTGGAACAGC CAGAAGGACA TCCTGGAGAG GACCCGAGCG GAGTTGGACA CGGTGTGCAG
            181 ACACAACTAC GAGGTGGCGT TCCGCGGGAT CTTGCAGAGG AGAGGTGAGC GTGGTTGACC
            241 CTCCGTGAGG CGCACCCTTG GCCGGGACCC CGAGTCTCTG TGCCGGGAGG GGCGATGGGG
            301 GCGCGGCCTC TGAAATCTGA GCCTCGTTCA TTCCACGCCA GGAGACAGGA GGCGGCGGAG
            361 GGGGTGGTGG GGGCAGGTGC ACCGGAGGAG CGGGGACCTA GGGCAGAGCA GGGGGACAAG
            421 CAGAGTTGAC TAGGCTGCCT AGTGTCCCCC CAGCCTCCCC GTCCGTCGGC TTCGTCCTCT
            481 GCTCTGCAGG TTTCTCGCCT TCTGCCTTGT GCGTTTGCCT CCTCGTGCCT TACCTTTACT
            541 AAGCAGTTCT CTCTGCCCCC AGTGCCCACC CTCTTCCCCT GCCCGCCTGC CCCGCTAGTA
            601 CTGCCCCACC CAGCAAGGCC CACGTACGCA GCTCGCGCCT CAGGAAGCTT CAGGCTTGGC
            661 CTGNNGGAGT TAGGGCTGCC CCACAACTGC GCTCAGGGCA TCCNGCAATT ACAGTTGCGA
            721 AATAAGANAT TTTGACTTTT TGGCTTCNAA TCNTTATTCA TCGNAATTCT GNTTTCTTAA
            781 ATGNCTATCA CTCATGGCGG ANATCTTTGA NGNGANANAC NGNTTNATTA TTGCNNGCTN
            841 GGNNCCTGAC ACNTTGACNG GTATGNGNNA TGANCTCNNN GATCTNNNNN TAANTTCNTG
            901 AANNANTGNN CNCNGCTGNN NNNNCNNTNN NNGNTCNANA AAAAANNNNA NGCTNNCAAN
            961 NNGNANNNNA NNNNNNACNT TNCNANCNTT TNGNNTNNNN NNTNCNAANN NANNTNNTNN
          1021 NNNNNNNN
          //
          Primer: 0601-exon2_R2
          Region: insert mid(c region-exon 2 5')
          Sequence file: RDB01230_B1Bfd.seq check
          >01230_01230_B1Bf_1_0601-exon2_R2_L96_D01_04_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNCCCNCNNC GCTGTCGNAN CGCACGTCCT CCTCTCGGTT
             61 ATAGATGTAT CTGGTCACAT AACGCACGCG CTCCGTCCCA TTGGTGAAGT AGCACATGGC
            121 CTTAAACTGG AGCACGAAAT CCTCTGCGGG GAATCACCGG CCGGTCAGTC AGGCCCCAGC
            181 CTGGCCCTAG CCCGGCCGCC CCCGCAGCCG CCGCCCTGAC CCGGCCCGGA GCTGTGGAAC
            241 CGCCCGCGCG ACCTCCAGTT CCCGCCTGCC CGTGTCTGGC GCTCCAGAAC TGGGATCCTC
            301 CCGGCGGCTC TGCTCAGCCC TGCCCGCCCT CTCTGGGGGC TTCGGGAATC TGCCTTCCTA
            361 CAGCCAGGAA AGGGAGGAAA GCCCTGTCCC TGCCTGAGCC AGTGAACTCA GCGAAGAGGC
            421 AGTCGGGCTG ATTTTACATG GACCTCTACA CTTAGAGGGA TCAGGGCGTT CTCGCATGAA
            481 ATCCCATTTT TCATGAAGCT CCTGGATACC TCAGAGATAT AGTTAATCCA CAAAAACTTG
            541 AGAGTTCAAG GGAATGACGA GACAGGTCCA GGAANTAAGC CTGTCCTCGT TCTGATACGC
            601 GTATTCTCTT GGTCCCTGGG TAAANNACCT TCCTTCCNNT GNCTGGANTT ACCNTANCNN
            661 GTNCNGTTGA NGNTNNNTCC CNTCTANGTT AGAAANGANN NACNNNTCTN NNNNCTANNN
          721 NNNAANNNNT TNNNTNNNNN GNN
          //
          Primer: 0601b-B_F
          Region: insert mid(b region 5',c region 3')
          Sequence file: RDB01230_B1Bfe.seq check
          >01230_01230_B1Bf_1_0601b-B_F_C01_03_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNGNN NNNNNTTGAG CTCNTACCNC ATACCAGTCA ATGTGTCAGG
             61 TACCAAGCAT GCAATAATTA AACAGTCTCT CGCCTCAAAG ATCTCCGCCA TGAGTGATAG
            121 CCATTTAAGA AAACAGAATT ACGATGAATA ATGATTTGAA GCCAAAAAGT CAAAATATCT
            181 TATTTCGCAA CTGTAATTGC TGGATGCCCT GAGCGCAGTT GTGGGGCAGC CCTAACTCCA
            241 CCAGGCCAAG CCTGAAGCTT CCTGAGGCGC GAGCTGCGTA CGTGGGCCTT GCTGGGTGGG
            301 GCAGTACTAG CGGGGCAGGC GGGCAGGGGA AGAGGGTGGG CACTGGGGGC AGAGAGAACT
            361 GCTTAGTAAA GGTAAGGCAC GAGGAGGCAA ACGCACAAGG CAGAAGGCGA GAAACCTGCA
            421 GAGCAGAGGA CGAAGCCGAC GGACGGGGAG GCTGGGGGGA CACTAGGCAG CCTAGTCAAC
            481 TCTGCTTGTC CCCCTGCTCT GCCCTAGGTC CCCGCTCCTC CGGTGCACCT GCCCCCACCA
            541 CCCCCTCCGC CGCCTCCTGT CTCCTGGCGT GGAATGAACG AGGCTCAGAT TTCAGAGGCC
            601 GCGCCCCCAT CGCCCCTCCC GGCACAGAGA CTCGGGGTCC CGGCCNAGGG TGCGCCTCAC
            661 GGANGGTCAA CCACGCTCAC CTCTCCTCTG NAAGANCCCG CGNAANGCCN NCTCNNANTT
          721 GNGTCTGNNC ACCNNNNNCA NNTNNNNTCN GNNNNNNN
          //
          Primer: B10401-T_F
          Region: insert mid(b region 3',e region 5')
          Sequence file: RDB01230_B1Bff.seq check
          >01230_01230_B1Bf_1_B10401-T_F_F01_06_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN CTGANTGANA GAGCTTATTG CAGGANACCC TTACCATTTA
             61 TCATTGTCTC ACTGGCTCCT TTCCAAAAGC TTCCTCCATT AAGAGGGTCA GAGCCTTGGC
            121 CTCCTTGCCT TCTAGTGACA ATTTTCTTTG TTTTAGGGGA TTTTAAATTA GGGTACTTAA
            181 GGCCTTGAAG AACATGAGTG GTAAGAGAAT ATAACTCTAA TTAAGTCACA TGTGTCATTT
            241 TCCTTTGGGG TGAAAGAGTG GCTGTTTGTG TAATGAGACC TTTCTCTGCA TAACTTCCTT
            301 TTGTAAGACC TCAAGGGCCT CCACCAGCAG GTGATATTTC AGCCATGAGC CAGTGTGGGG
            361 GGGCACAGGT GTAAGAGGGA AGAGCATGAG CTGAATGCAC CTGACCACAA TGGTCTCTGT
            421 TCATGGTATA TTTGCTGCTA TGAGGATCAA GACTTAGGGT CGAAGTTTGC CAGTTTCTAG
            481 GAATCTCCAG AGGTTGTTCC CCAGAACCAA GCCTTAACTT TGGTGGTATC TTCTTGTGAA
            541 ATGTGAAGCC AGAACCACAG CTTAAATGTT AGACAAGAGG ANGANGCCCA CTTTGTGCCA
            601 CATGTTGGTG GCTACTGCCT GTANGCATTT TCCAGTGACT GAAAGANGCT GCTAGTGGTA
            661 NGGANGANGN ATCATCCAAT TTTCNNAAAA NANTGANNCC TTNNNANTCC CNNNNANNAN
          721 TANNNNCTNN NNNNNCNNNN NNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Senju, S., Allele-specific expression of the cytoplasmic exon of HLA-DQB1 gene. Immunogenetics, 36, 319-325 (1992). PMID 1644449.

          2022.02.09

          GNP_filter3_RDBDEP_html_220112.pl