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Tatsuo Fukagawa's web page in English / in Japanese
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Catalog # | Name of clone | Running title | Publication |
RDB19231 | pAID-EF1a- linearizing in pX330 | Expression vector of Cas9 protein and sgRNA to linearize to pAID-EF1 alpha plasmids in vivo. | Nishimura, K., A super-sensitive auxin-inducible degron system with an engineered auxin-TIR1 pair. Nucleic Acids Res. 48 (18): e108 (2020). PMID 32941625. (original). [link to RRC of NBRP] |
RDB19230 | pAID1.2-CMV-linearizing in pX330 | Expression vector of Cas9 protein and sgRNA to linearize pAID-CMV plasmids in vivo. | Nishimura, K., A super-sensitive auxin-inducible degron system with an engineered auxin-TIR1 pair. Nucleic Acids Res. 48 (18): e108 (2020). PMID 32941625. (original). [link to RRC of NBRP] |
RDB19229 | pAIDFA-EF1a-NmScarlet-mAID | Expresson vector to construct mScarlet-mAID tagged target gene and OsTIR1 mutant (F74A) simultaneously by EF1 alpha promoter for the super-sensitive AID system. | Nishimura, K., A super-sensitive auxin-inducible degron system with an engineered auxin-TIR1 pair. Nucleic Acids Res. 48 (18): e108 (2020). PMID 32941625. (original). [link to RRC of NBRP] |
RDB19228 | pAIDFA-CMV-NmScarlet-mAID | Expresson vector to construct mScarlet-mAID tagged target gene and OsTIR1 mutant (F74A) simultaneously by CMV promoter for the super-sensitive AID system. | Nishimura, K., A super-sensitive auxin-inducible degron system with an engineered auxin-TIR1 pair. Nucleic Acids Res. 48 (18): e108 (2020). PMID 32941625. (original). [link to RRC of NBRP] |
RDB19227 | pAID1.2-EF1a-NmScarlet-mAID | Expresson vector to construct mScarlet-mAID tagged target gene and OsTIR1 (WT) simultaneously by EF1 alpha promoter for the super-sensitive AID system. | Nishimura, K., A super-sensitive auxin-inducible degron system with an engineered auxin-TIR1 pair. Nucleic Acids Res. 48 (18): e108 (2020). PMID 32941625. (original). [link to RRC of NBRP] |
RDB19226 | pAID1.2-CMV-NmScarlet-mAID | Expresson vector to construct mScarlet-mAID tagged target gene and OsTIR1 (WT) simultaneously by CMV promoter for the super-sensitive AID system. | Nishimura, K., A super-sensitive auxin-inducible degron system with an engineered auxin-TIR1 pair. Nucleic Acids Res. 48 (18): e108 (2020). PMID 32941625. (original). [link to RRC of NBRP] |
♦ No Approval Forms Required for Fluorescent Protein Genes Developed by Dr. Atsushi Miyawaki
♦ RIKEN BRC will be closed from December 28, 2024 through January 5, 2025 due to New Year's holidays.
♦ A bicistronic cell cycle reporter, Fucci2a (Sep 17, 2024)
♦ Monomeric Fluorescent Protein Resource, mStayGold (Dec 18, 2023)
♦ Visualization of Organelles update (Dec 18, 2023)
♦ Development of two mouse strains conditionally expressing bright luciferases (Sep 08, 2023)
♦ Autophagy and Mitophagy Updates (Aug 16, 2023)
♦ High intensity forms of luciferase and luminescent proteins from various organisms (BRC RESOURCE NEWS) (Apr 28, 2023)
♦ Plasmid of Cas9 expression/mRNA production, evaluation of the genome edit efficiency, and Knock-in donors and tags
♦ Fucci cell cycle indicator, Calcium sensor and Fluorescent and Luminescent protein resources
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2024.10.24
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