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Tatsuo Fukagawa's Resource

Tatsuo Fukagawa's web page in English /  in Japanese

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Catalog #Name of cloneRunning titlePublication
RDB19231pAID-EF1a- linearizing in pX330Expression vector of Cas9 protein and sgRNA to linearize to pAID-EF1 alpha plasmids in vivo.Nishimura, K., A super-sensitive auxin-inducible degron system with an engineered auxin-TIR1 pair. Nucleic Acids Res. 48 (18): e108 (2020). PMID 32941625. (original). [link to RRC of NBRP]
RDB19230pAID1.2-CMV-linearizing in pX330Expression vector of Cas9 protein and sgRNA to linearize pAID-CMV plasmids in vivo.Nishimura, K., A super-sensitive auxin-inducible degron system with an engineered auxin-TIR1 pair. Nucleic Acids Res. 48 (18): e108 (2020). PMID 32941625. (original). [link to RRC of NBRP]
RDB19229pAIDFA-EF1a-NmScarlet-mAIDExpresson vector to construct mScarlet-mAID tagged target gene and OsTIR1 mutant (F74A) simultaneously by EF1 alpha promoter for the super-sensitive AID system.Nishimura, K., A super-sensitive auxin-inducible degron system with an engineered auxin-TIR1 pair. Nucleic Acids Res. 48 (18): e108 (2020). PMID 32941625. (original). [link to RRC of NBRP]
RDB19228pAIDFA-CMV-NmScarlet-mAIDExpresson vector to construct mScarlet-mAID tagged target gene and OsTIR1 mutant (F74A) simultaneously by CMV promoter for the super-sensitive AID system.Nishimura, K., A super-sensitive auxin-inducible degron system with an engineered auxin-TIR1 pair. Nucleic Acids Res. 48 (18): e108 (2020). PMID 32941625. (original). [link to RRC of NBRP]
RDB19227pAID1.2-EF1a-NmScarlet-mAIDExpresson vector to construct mScarlet-mAID tagged target gene and OsTIR1 (WT) simultaneously by EF1 alpha promoter for the super-sensitive AID system.Nishimura, K., A super-sensitive auxin-inducible degron system with an engineered auxin-TIR1 pair. Nucleic Acids Res. 48 (18): e108 (2020). PMID 32941625. (original). [link to RRC of NBRP]
RDB19226pAID1.2-CMV-NmScarlet-mAIDExpresson vector to construct mScarlet-mAID tagged target gene and OsTIR1 (WT) simultaneously by CMV promoter for the super-sensitive AID system.Nishimura, K., A super-sensitive auxin-inducible degron system with an engineered auxin-TIR1 pair. Nucleic Acids Res. 48 (18): e108 (2020). PMID 32941625. (original). [link to RRC of NBRP]

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No Approval Forms Required for Fluorescent Protein Genes Developed by Dr. Atsushi Miyawaki
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A bicistronic cell cycle reporter, Fucci2a (Sep 17, 2024)
Monomeric Fluorescent Protein Resource, mStayGold (Dec 18, 2023)
Visualization of Organelles update (Dec 18, 2023)
Development of two mouse strains conditionally expressing bright luciferases (Sep 08, 2023)
Autophagy and Mitophagy Updates (Aug 16, 2023)
High intensity forms of luciferase and luminescent proteins from various organisms (BRC RESOURCE NEWS) (Apr 28, 2023)
Plasmid of Cas9 expression/mRNA production, evaluation of the genome edit efficiency, and Knock-in donors and tags
Fucci cell cycle indicator, Calcium sensor and Fluorescent and Luminescent protein resources
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2024.10.24

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