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pAID1.2-CMV-linearizing in pX330 (#RDB19230)

Expression vector of Cas9 protein and sgRNA to linearize pAID-CMV plasmids in vivo.

Clone info. Expression vector of Cas9 protein and sgRNA to linearize pAID-CMV plasmids in vivo.
Vector backbone pX330-U6-Chimeric_BB-CBh-hSpCas9 (plasmid)
Size of vector backbone 4.3 kb
Selectable markers Ampicillin
Depositor|Developer Fukagawa, Tatsuo |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Nishimura, K. et al. Nucleic Acids Res. 48 (18): e108, 2020).
Remarks
The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.
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提供依頼書  [依頼書の記入例 ]
専用MTA（CRISPR/Cas9内包遺伝子材料専用 非営利学術目的）をお使いください [Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Nishimura, K. et al. Nucleic Acids Res. 48 (18): e108, 2020)。
備考
本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

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RDB19230 pAID1.2-CMV-linearizing in pX330 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pAID1.2-CMV-linearizing in pX330 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19230).

Reference section:

Nishimura, K., Yamada, R., Hagihara, S., Iwasaki, R., Uchida, N., Kamura, T., Takahashi, K., Torii, K.U., Fukagawa, T., A super-sensitive auxin-inducible degron system with an engineered auxin-TIR1 pair. Nucleic Acids Res. 48 (18): e108 (2020). PMID 32941625. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB19230_B1H2p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: U6pro_F3 (Pr0648)
Region: U6 pro,sgRNA for pAID-CMV,CMV en,chicken β-actin pro
Sequence file: RDB19230_B1H2a.seq check
>D08293A5_B1H2_2_U6pro_F3_E01_13_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNGG NNTATNNTAT GCTTACCGTA ACTTGNAAGT ATTTCGATTT
   61 CTTGGCTTTA TATATCTTGT GGAAAGGACG AAACACCGCC AAGTTCGGGT GAAGGCCCGT
  121 TTAAGAGCTA TGCTGGAAAC AGCATAGCAA GTTTAAATAA GGCTAGTCCG TTATCAACTT
  181 GAAAAAGTGG CACCGAGTCG GTGCTTTTTT GTTTTAGAGC TAGAAATAGC AAGTTAAAAT
  241 AAGGCTAGTC CGTTTTTAGC GCGTGCGCCA ATTCTGCAGA CAAATGGCTC TAGAGGTACC
  301 CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT
  361 GACGTCAATA GTAACGCCAA TAGGGACTTT CCATTGACGT CAATGGGTGG AGTATTTACG
  421 GTAAACTGCC CACTTGGCAG TACATCAAGT GTATCATATG CCAAGTACGC CCCCTATTGA
  481 CGTCAATGAC GGTAAATGGC CCGCCTGGCA TTGTGCCCAG TACATGACCT TATGGGACTT
  541 TCCTACTTGG CAGTACATCT ACGTATTAGT CATCGCTATT ACCATGGTCG AGGTGAGCCC
  601 CACGTTCTGC TTCACTCTCC CCATCTCCCC CCCCTCCCCA CCCCCAATTT TGTATTTATT
661 TATTTTTTAA TTATTTTGTG CAGCGATGGG GGCGGGGGGG GGGGGGGNNN NNNNNNN
//
Primer: Cas9-C_F2 (Pr1051)
Region: Cas9 3',nucleoplasmin NLS,bGHpA
Sequence file: RDB19230_B1H2b.seq check
>19230_19230_B2DkF_2_Cas9-C_F2_H01_08_ABI08.ab1
    1 NNNNNNNNNN NNNNNNGNNN CCNCCCTGNT CNNCCNGAGC ATCACCGGCC TGTACGAGAC
   61 ACGGATCGAC CTGTCTCAGC TGGGAGGCGA CAAAAGGCCG GCGGCCACGA AAAAGGCCGG
  121 CCAGGCAAAA AAGAAAAAGT AAGAATTCCT AGAGCTCGCT GATCAGCCTC GACTGTGCCT
  181 TCTAGTTGCC AGCCATCTGT TGTTTGCCCC TCCCCCGTGC CTTCCTTGAC CCTGGAAGGT
  241 GCCACTCCCA CTGTCCTTTC CTAATAAAAT GAGGAAATTG CATCGCATTG TCTGAGTAGG
  301 TGTCATTCTA TTCTGGGGGG TGGGGTGGGG CAGGACAGCA AGGGGGAGGA TTGGGAAGAG
  361 AATAGCAGGC ATGCTGGGGA GCGGCCGCAG GAACCCCTAG TGATGGAGTT GGCCACTCCC
421 TCTCTGCGCG CTC
//
Primer: Cas9-N_R1 (Pr0650)
Region: Cas9 5',SV40 NLS,3 x FLAGhybrid intron
Sequence file: RDB19230_B1H2c.seq check
>D08293A5_B1H2_2_Cas9-N_R1_A01_01_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNGNAN TCGTCGGTGN NCACGGCCCN GCCCACAGAG
   61 TTGGTGCCGA TGTCCAGGCC GATGCTGTAC TTCTTGTCGG CTGCTGGGAC TCCGTGGATA
  121 CCGACCTTCC GCTTCTTCTT TGGGGCCATC TTATCGTCAT CGTCTTTGTA ATCAATATCA
  181 TGATCCTTGT AGTCTCCGTC GTGGTCCTTA TAGTCCATGG TGGCACCGGT CCAACCTGAA
  241 AAAAAGTGAT TTCAGGCAGG TGCTCCAGGT AATTAAACAT TAATACCCCA CCAACCAACC
  301 ATCCCTTAAA CCCTTACCTC TTGCTCAGCT AATTACAGCC CGGAGGAGAA GGGCCGTCCC
  361 GCCCGCTCAC CTGTGGGAGT AACGCGGTCA GTCAGAGCCG GGGCGGGCGG CGCGAGGCGG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Nishimura, K., A super-sensitive auxin-inducible degron system with an engineered auxin-TIR1 pair. Nucleic Acids Res. 48 (18): e108 (2020). PMID 32941625. [link to RRC of NBRP]