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50/H05 - S. pombe Postgenome Database Clone

 Plasmid request
Clone ID at the S. pombe Postgenome Database 50/H05
NCBI Gene ID 2542180
Gene Symbol SPAC589.07c
Protein Name WD repeat protein Atg18
Synonym
RefSeq Protein NP_594055.1
PomBase(1) SPAC589.07c
KEGG(2) spo:SPAC589.07c
Depositor-supplied data(3) 50H05
Note 87A:G(4)

(1) The PomBase, funded by the Wellcome Trust and is run by a consortium comprising the University of Cambridge, the European Bioinformatics Institute and University College London.

(2) The Kyoto Encyclopedia of Genes and Genomes of the Kanehisa Laboratories in the Bioinformatics Center of Kyoto University and the Human Genome Center of the University of Tokyo.

(3) Primer sequences for constructing an entry clone and known sequence variation are available at this site, the S. pombe Postgenome Database of the Chemical Geneitcs Laboratory, RIKEN.

(4) Remarks by the Depositor.

Please note that the information was bioinformatically annotated and that this information may not reflect the most recent annotation of the reference sequence. You should carefully check the insert sequence provided by us to make sure that it matches the sequence you expect.
Gene Engineering Division has digested by restriction enzymes and sequenced a portion of the resource for verification. The result is shown below if available.

Please note that they are GENOMIC clones.

Entry vector

Catalog number SPW020173
Clone name SpENT50H05
Clone status This vector is NOT "pDONR201". | The entry vector is not pDONR201.
Seq File
(by primer A)
Seq File
(by primer B)
Seq File
(by primer C)
PDF File
SpENT50H05a.seq SpENT50H05b.seq   SpENT50H05.pdf

FFH Vector (FLAG2-His6-tag clone)

Catalog number SPW060173
Clone name SpFFH50H05
Clone status confirmation required

YFH Vector (YFP-FLAG-His6-tag clone)

Catalog number SPW100173
Clone name SpYFH50H05
Clone status confirmation required
user report - reference Sun, L.L., Global analysis of fission yeast mating genes reveals new autophagy factors. PLoS Genet. 9 (8): e1003715 (2013). PMID 23950735.

Reference of clones: Matsuyama, A., Nature Biotech., 24, 841-847 (2006).

Please visit Data Sheet for the information of vectors and sequence primers.


2018.10.13

pombe_clone_primer_ver2_160708_xlsx.csv - GNP_filter3_pombe_html_171016.pl