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29/D03 - S. pombe Postgenome Database Clone

Available from Ordering information ordering information
Clone ID at the S. pombe Postgenome Database 29/D03
NCBI Gene ID 2539461
Gene Symbol ppk36
Protein Name serine/threonine protein kinase Ppk36
Synonym SPCC63.08c|atg1
RefSeq Protein NP_587982.1
PomBase(1) link 01
KEGG(2) link 01
Depositor-supplied data(3) link 01
Note  

Gene Engineering Division has digested by restriction enzymes and sequenced a portion of the resource for verification. The result is shown below if available.

Entry vector

Catalog number SPW011675
Clone name SpENT29D03
Clone status confirmation required

FFH Vector(5)

Catalog number SPW051675
Clone name SpFFH29D03
Clone status confirmation required

YFH Vector(6)

Catalog number SPW091675
Clone name SpYFH29D03
Clone status Known as cytoplasmic puncta marker (Matsuyama, A., Nat Biotechnol 24(7):841?847, 2006; Li, G. Proc. Natl. Acad. Sci. U.S.A. 114 (3): E376-E385, 2017).
user report - reference Li, G., Characterization of cytopathic factors through genome-wide analysis of the Zika viral proteins in fission yeast. Proc. Natl. Acad. Sci. U.S.A. 114 (3): E376-E385 (2017). PMID 28049830.
user report - reference Sun, L.L., Global analysis of fission yeast mating genes reveals new autophagy factors. PLoS Genet. 9 (8): e1003715 (2013). PMID 23950735.

Please note that they are GENOMIC clones.
Please visit plasmidData Sheet for the information of vectors and sequence primers.

(1) The PomBase, funded by the Wellcome Trust and is run by a consortium comprising the University of Cambridge, the European Bioinformatics Institute and University College London.

(2) The Kyoto Encyclopedia of Genes and Genomes of the Kanehisa Laboratories in the Bioinformatics Center of Kyoto University and the Human Genome Center of the University of Tokyo.

(3) Primer sequences for constructing an entry clone and known sequence variation are available at this site, the S. pombe Postgenome Database of the Chemical Geneitcs Laboratory, RIKEN.

(5) FLAG2-His6-tag clone.

(6) YFP-FLAG-His6-tag clone.

(7) Reference of clones: Matsuyama, A., Nature Biotech., 24, 841-847 (2006).


2017.05.15

pombe_clone_primer_ver2_160708_xlsx.csv - GNP_filter3_pombe_html_160723.pl