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pEGFP-C1-Rab27B-T23N (#RDB21179)

Expression vector of mouse Rab27B constitutive negative mutant (T23N)


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
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Publication Fukuda, M., J. Biol. Chem., 277, 12432-12436 (2002). PMID 11856727. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Expression vector of mouse Rab27B constitutive negative mutant (T23N)
Comment Expression was confirmed by the depositor with immunoblotting.
Vector backbone pEGFP-C1 (plasmid)
Selectable markers Kanamycin (E. coli), Neomycin (mammalian cell)
Growth conditions LB, Kanamycin, 37℃
Gene/insert name mouse Rab27B cDNA.
Depositor|Developer Fukuda, Mitsunori |

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Fukuda, M. et al. J. Biol. Chem. 277 (14): 12432-12436 2002.). For use of the BIOLOGICAL RESOURCE by a for-profit organization or use of the BIOLOGICAL RESOURCE for for-profit research by not-for-profit organization, the RECIPIENT must obtain a prior written consent from the Tohoku University(lif-soumu@grp.tohoku.ac.jp).
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Fukuda, M. et al. J. Biol. Chem. 277 (14): 12432-12436 2002.)。営利機関の利用もしくは非営利学術機関による営利目的研究利用の場合は、 東北大学(lif-soumu@grp.tohoku.ac.jp)の許可を得ること。

Catalog # Resource name Shipping form Fee
RDB21179 pEGFP-C1-Rab27B-T23N DNA solution

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-Rab27B-T23N was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB21179).

Reference section:

Fukuda, M., Kuroda, T.S., Mikoshiba, K., Slac2-a/melanophilin, the missing link between Rab27 and myosin Va: implications of a tripartite protein complex for melanosome transport. J. Biol. Chem., 277, 12432-12436 (2002). PMID 11856727. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Fukuda, M., Slac2-a/melanophilin, the missing link between Rab27 and myosin Va: implications of a tripartite protein complex for melanosome transport. J. Biol. Chem., 277, 12432-12436 (2002). PMID 11856727. [PubMed] [Article] [RRC of NBRP]
reference Ishida, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci., 125 (Pt 21): 5177-5187 (2012). PMID 22854043. [PubMed] [Article] [RRC of NBRP]