Resource data sheet
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pCSII-CLDN5 (#RDB21018)

Expression vector of human claudin-5 (CLDN5). Compatible with lentiviral transduction (3rd generation).


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Rahman, A., Int. J. Mol. Sci. 22 (7): 3774 (2021). PMID 33917356. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Expression vector of human claudin-5 (CLDN5). Compatible with lentiviral transduction (3rd generation).
Comment BleoR is not applicable for lentivirus transduction, as it is located outside the packaging sequence.
Vector backbone CSII-EF-MCS-IRES2-Venus (RDB04384) (plasmid)
Selectable markers Ampicillin (E. coli). Please note that BleoR marker is not included in the lentivirus produced from this vector.
Growth conditions LB, 37℃
Gene/insert name human CLDN5 cDNA.
Depositor|Developer Sugimoto, Kotaro |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Rahman, A. et al., Int. J.Mol. Sci., 22(7):3774, 2021) For use of the BIOLOGICAL RESOURCE by a for-profit organization or for use of the BIOLOGICAL RESOURCE for for-profit research by not-for-profit organization, the RECIPIENT must obtain a prior written consent from the FUKUSHIMA
MEDICAL UNIVERSITY (liaison@fmu.ac.jp).
Additional terms and conditions:
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Rahman, A. et al., Int. J.Mol. Sci., 22(7):3774, 2021)。営利機関の利用もしくは非営利学術機関による営利目的研究利用の場合は、福島県立医科大学 (liaison@fmu.ac.jp) の許可を得ること。
付加的使用条件：
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee
RDB21018 pCSII-CLDN5 DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

How to cite this biological resource

Materials & Methods section:

The pCSII-CLDN5 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB21018).

Reference section:

Rahman, A., Kobayashi, M., Sugimoto, K., Endo, Y., Kojima, M., Furukawa, S., Watanabe, T., Soeda, S., Hashimoto, Y., Fujimori, K., Chiba, H., Reduced Claudin-12 Expression Predicts Poor Prognosis in Cervical Cancer. Int. J. Mol. Sci. 22 (7): 3774 (2021). PMID 33917356. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Rahman, A., Reduced Claudin-12 Expression Predicts Poor Prognosis in Cervical Cancer. Int. J. Mol. Sci. 22 (7): 3774 (2021). PMID 33917356. [PubMed] [Article] [RRC of NBRP]