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pET-24b(+)_IFNG variant(I45K/F55K/F83K) (#RDB21009)

Expression vector of human monomeric interferon gamma variant (I45K/F55K/F83K). His-tag at C-terminus. IPTG-inducible. pET-24b(+) vector.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Goto, Y., J. Biol. Chem. 300 (7): 107464 (2024). PMID 38879015. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Alternative name monoIFNG-5 plasmid
Clone info. Expression vector of human monomeric interferon gamma variant (I45K/F55K/F83K). His-tag at C-terminus. IPTG-inducible. pET-24b(+) vector. See the experimental procedures(Fig3E).
Vector backbone pET-24b(+) (plasmid)
Selectable markers Kanamycin
Growth conditions LB+Kanamycin 37℃
Gene/insert name human IFNG cDNA.
Depositor|Developer Honda, Shinya |

Distribution information

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Terms and conditions for distribution For use of the BIOLOGICAL RESOURCE by a for-profit organization or for useof the BIOLOGICAL RESOURCE for for-profit research by not-for-profitorganization, the RECIPIENT must obtain a prior written consent from the NationalInstitute of Advanced Industrial Science and Technology.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Goto ,Y. et al., J. Biol. Chem., 300(7):107464, 2024.)。営利機関の利用もしくは非営利学術機関による営利目的研究利用の場合は、国立研究開発法人産業技術総合研究所の許可を得ること。

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RDB21009 pET-24b(+)_IFNG variant(I45K/F55K/F83K) DNA solution

How to cite this biological resource

Materials & Methods section:

The pET-24b(+)_IFNG variant(I45K/F55K/F83K) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB21009).

Reference section:

Goto, Y., Miyafusa, T., Honda, S., Designing monomeric IFNγ: The significance of domain-swapped dimer structure in IFNγ immune responses. J. Biol. Chem. 300 (7): 107464 (2024). PMID 38879015. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Goto, Y., Designing monomeric IFNγ: The significance of domain-swapped dimer structure in IFNγ immune responses. J. Biol. Chem. 300 (7): 107464 (2024). PMID 38879015. [PubMed] [Article] [RRC of NBRP]